The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea

Document Type : Research Article

Authors

• Zeinabsadat Shahzeidi ¹
• Saeid Hesami Tackallou ¹
• Leila Amjad ²
• Hakimeh Zali ³
• Alireza Iranbakhsh ¹

¹ Biology, Faculty of Basic Sciences, Islamic Azad University, Sciences and Research Branch, Tehran, Iran

² Biology, IbnSina Faculty, Islamic Azad University Falaverjan Branch, Isfahan, Iran

³ Faculty of Medical Tissue Engineering, Shahid Beheshti University of Medical Sciences, Tehran, Iran

https://doi.org/10.22067/jhs.2021.71685.1077

Abstract

Introduction
 UV-C (254-280 nm) and 280-320 nm) UV-B, UV-A (320-390nm) wavelengths are irradiated with three ultraviolet strips and have detrimental effects on the growth of a number of plants. Ultraviolet light is an important non-living factor that can stimulate the production of secondary metabolites, including antioxidant compounds in plants. Ozone depletion and its consequences, including direct UV radiation on the planet and its effects on crops and medicinal plants, are among the topics that have received very little study. Ultraviolet light in nature occurs only at low intensities, but if the inhibitory effect of the ozone layer in the stratosphere is significantly the result of nitrogen and hydrocarbon oxides the weaker the halogen, the higher its amount.
Materials and Methods
 Portulaca oleracea seeds were prepared by Pakan Isfahan Company. The aim of this study was the effect of ultraviolet rays at different levels (UV-C: 0, 100, 200, 300, 400, 500, 600, and 700 nm) on the activity of photosynthetic pigments and biochemical traits of portulaca oleracea in factorial in a completely randomized design with three replications. After transferring the seeds of portulaca oleracea, the healthy and uniform seeds of this plant were sterilized in 15% sodium hypochlorite solution for 154 minutes and then washed thoroughly with distilled water and placed in a petri dish for germination. Moisture was supplied through filter paper soaked in distilled water. The seeds were planted in pots filled with cocopeat and perlite evenly and watered for 20 days with a half-strength Hoagland solution. Plants were grown for 20 days at a temperature of 30 ± 2 ° C and a light period of 8.16 (light / dark, respectively). Plants for one week, every other day, and for 3 minutes each time by two fluorescent lamps with a wavelength of 260 nm exposed to ultraviolet C (at a distance of 30 cm from the UV light source with an intensity of 27 (w / m2) were located. The traits studied in this study included chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, phenol, flavonoids, and antioxidant activity. In this study, the effect of ultraviolet light on the activity of photosynthetic pigments and biochemical traits of portulaca oleracea was investigated factorially in a completely randomized design with three replications.
Results and Discussion
 The results of the mean comparison showed that the UV treatment of chlorophyll a, b, total chlorophyll, carotenoid of portulaca oleracea was reduced compared to the control; However, UV treatment of portulaca oleracea significantly increased phenol, flavonoids, and antioxidants compared to the control. The effect of different doses of ultraviolet rays on phenol and portulaca oleracea antioxidants showed that the UV-C highest and lowest were 700 and 100 nm, respectively. Decreases in carotenoid content can result in either inhibition of pigment synthesis or their breakdown and degradation. The results of this report indicate significant changes in phenols and flavonoids as compounds it absorbed ultraviolet rays compared to control cells.
Conclusion
 It can be said that excessive exposure to radiation may affect chlorophyll levels by inhibiting chlorophyll biosynthesis or accelerating its degradation. Oxygen is an electron receptor in the electron transport system that produces energy from adenosine triphosphate (ATP) in the body. Under certain conditions, oxygen can be converted to a single electron, creating free radicals. When oxygen is converted to a single electron, it is called active oxygen (ROS). These free radicals cause oxidative stress in plants which oxidative stress leads to damage to macromolecules such as DNA, proteins and so on. Environmental stresses, including UV radiation, produce active oxygen species (ROS) such as hydrogen peroxide (H₂O₂), superoxide (O₂^-), and hydroxyl radicals (OH), which cause oxidative stress and cause damage to cells, such as DNA. And cause the destruction of these compounds. The plant contains compounds that act as active antioxidants and sweep away active oxygen. In the present study, the observed increase in phenols, flavonoids and antioxidants indicates an increase in the production of free radicals under ultraviolet radiation and shows that the production of these radicals is more than the plant's defense capacity and has caused damage to plant biological membranes. In summary, the application of controlled ultraviolet light stress can provide a new alternative strategy to increase the productivity of the portulaca oleracea plant. Modulating UV-C light in agricultural systems is a promising tool to increase crop production.
 

Keywords

• Antioxidant
• Extract
• Flavonoid
• Phenol

20.1001.1.20084730.1401.36.1.22.9

Main Subjects

• Medicinal Plants

References

1. Adetuyi F.O., Karigidi K.O., and Akintimehin E.S. 2020. Effect of postharvest UV-C treatments on the bioactive components, antioxidant and inhibitory properties of clerodendrum volubile leaves. Journal of the Saudi Society of Agricultural Sciences 119(1): 7-13. DOI: 10.1016/j.jssas.2018.03.005.
2. Agati G., Azzarello E., Pollastri S., and Tattini M. 2012. Flavonoids as antioxidants in plants: location and functional significance. Plant Science 196: 67-76. DOI: 10.1016/j.plantsci.2012.07.014.
3. Alexieva V., Sergiev I., Mapelli S., and Karanov E. 'The effect of drought andultraviolet radiation on growth and stressmarkers in pea and wheat'. Plant, Cell & Environment 24(12): 1337-1344. DOI:10.1046/j.1365-3040.2001. 00778. x.
4. Alothman M., Bhat R., and Karim A.A. 2009. UV radiation-induced changes of antioxidant capacity of fresh-cut tropical fruits. Innovative Food Science & Emerging Technologies 10(4):512-6.

DOI: 10.1016/j.ifset.2009.03.004.

5. Balouchi H.R., Modarres Sanavy S.A.M., Emam Y., and BarzeGar M.O.H.S.E.N. 2008. Effect of Water Deficit, Ultraviolet Radiation and Carbon Dioxide Enrichment on Leaf Qualitative Characters of Durum Wheat (Triticum turgidum). Isfahan University of Technology-Journal of Crop Production and Processing 12(45): 167-181. DOI: 20.1001.1.24763594.1387.12.45.15.2.
6. Bhat R, Sridhar K.R., and Tomita-Yokotani K. 2007. Effect of ionizing radiation on antinutritional features of velvet bean seeds (Mucuna pruriens). Food Chemistry 103: 860–866. DOI:10.1016/j.foodchem.2006.09.037.
7. Booij-James I.S., Dube S.K., Jansen M.A., Edelman M., and Mattoo A.K. 'Ultraviolet B radiation impacts light-mediated turnoverof the photosystem II reaction centerheterodimer in Arabidopsis mutants alteredin phenolic metabolism'. Plant Physiology124(3): 1275-1284.DOI:10.1104/pp.124.3.1275.
8. Bravo L. 1998. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Reviews 56(11): 317-33. DOI: 10.1111/j.1753-4887. 1998.tb01670.x.
9. Casati P., Lara M.V., and Andreo C.S. 2002. Regulation of enzymes involved in C 4 photosynthesis and the antioxidant metabolism by UV-B radiation in Egeria densa, a submersed aquatic species. Photosynthesis Research 71(3): 251-64. DOI:10.1023/A:1015543208552.
10. Chen J., Xiao Q., Wang C., Wang W.H., Wu F.H., He B.Y., Zhu Z, Ru Q.M., Zhang L.L., and Zheng H.L. 2014. Nitric oxide alleviates oxidative stress caused by salt in leaves of a mangrove species, Aegiceras Aquatic Botany 117: 41-47. DOI: 10.1016/j.aquabot.2014.04.004.
11. Costa L., Vicente A.R., Civello P.M., Chaves A.R., and Martínez G.A. 2006. UV-C treatment delays postharvest senescence in broccoli florets. Postharvest Biology and Technology 39(2): 204-210. DOI:10.1016/j.postharvbio.2005.10.012.
12. Hollosy F. 2002. Effects of ultraviolet radiation on plant cells. Micron 33: 179-197. DOI:10.1016/S0968-4328(01)00011-7.
13. Jahanbakhsh H., Secondary teacher S., A., M. Qanati F., Tavakoli M., and Panahi A. 2014. Evaluation of grain yield and quality traits of sweet corn (Zea mays var. Sacarata) under treatments of dehydration, ultraviolet radiation and carbon dioxide increase. Iranian Crop Science 45 (3): 355-344. DOI:10.22059/ijfcs.2014.53530.
14. Khalili M., Razavizadeh R., and Forghani A. 2019. Changes in pigments and secondary metabolites ofArtichoke seedlings in response to UV rays and sampling time in vitro. Plant Biology of Iran (2) 11: 22. DOI: 10.22108/ijpb.2019.116190.1146.
15. Krizek D.T., Britz S.J., and Mirecki R.M. 1998. Inhibitory effects of ambient levels of solar UV‐A and UV‐B radiation on growth of cv. New Red Fire lettuce. Physiologia Plantarum 103: 1-7. DOI:10.1034/j.1399-3054.1998. 1030101.x.
16. Li P., Yu X., and Xu B. 2017. Effects of UV-C light exposure and refrigeration on phenolic and antioxidant profiles of subtropical fruits (Litchi, Longan, and Rambutan) in different fruit forms. Journal of Food Quality DOI: 10.1155/2017/8785121.
17. Lichthentaler H. 1987. Chlorophyll and carotenoids-pigments of photosynthetic biomembranes. In: Methods in Enzymology (eds. Colowick, S. P. and Kaplan, N. O.) Academic Press, Sandiego, New York. DOI: 10.1016/0076-6879(87)48036-1.
18. Mahdavian K., Ghorbanli M., and Kalantari K.M. 2008. The effects of ultraviolet radiation on the contents of chlorophyll, flavonoid, anthocyanin and proline in Capsicum annuum Turkish Journal of Botany 32(1): 25-33.
19. Mandal S.M., Chakraborty D., and Dey S. 2010. Phenolic acids act as signaling molecules in plant-microbe symbioses. Plant Signaling & Behavior 5(4): 359-68. DOI:10.4161%2Fpsb.5.4.10871.
20. Nanjo F., Goto K., Seto R., Suzuki M., Sakai M., and Hara Y. 1996. Scavenging effects of tea catechins and their derivatives on 1, 1-diphenyl-2-picrylhydrazyl radical. Free Radical Biology and Medicine 21(6): 895-902. DOI: 10.1016/0891-5849(96)00237-7.
21. Papoutsis K., Quan V., Pristijono V.P., Golding J.B., Bowyer M.C., Scarlett C.J., and Stathopoulos C.E. 2016. Enhancing the total phenolic content and antioxidantsof lemon pomace aqueous extracts by applying uv-c irradiation to the dried powder. Foods 5(55): 1–10. DOI: 10.3390/foods5030055.
22. Perkins-Veazie P., Collins J.K., and Howard L. 2008. Blueberry fruit response to postharvest application of ultraviolet radiation. Posth. Biology Technology 47(3): 280–285. DOI:10.1016%2Fj.postharvbio.2007.08.002.
23. Pietta P.G. 2000. Flavonoids as antioxidants. Journal Natural Production 63(7): 1035-42. DOI: 10.1021/np9904509.
24. Pinheiro J., Alegria C., Abreu M., Goncalves E.M., and Silva C.L.M. 2015. Use of UV-C postharvest treatment for extend1ing fresh whole tomato (Solanum lycopersicum, cv. Zinac) shelf-life. Journal Food Science Technology 52(8): 5066–5074. DOI: 10.1007%2Fs13197-014-1550-0.
25. Pourakbar L., and Abedzadeh M. 2014. The effect of UVB-C and UV-C rays on the activity of antioxidant enzymes in Lemongrass (Melissa officinalis) and the effect of salicylic acid in reducing the stress caused by ultraviolet rays. Iranian Journal of Plant Biology 21: 23-34. DOI:20.1001.1.20088264.1393.6.21.4.9.
26. Publishing Sh., Turkzadeh M., Manouchehri Kalantari Kh., and Ghorbanli M.L. 2005. Effect of different UV bands on pigments in soybean leaf (Glycine max). Biology of Iran 18(1): 77-84.
27. Razavizadeh R., and Komatsu S. 'Changesin essential oil and physiological parametersof callus and seedlings of Carum copticum L.under in vitro drought stress'. Journal of Food Measurement and Characterization 12(3): 1581-1592. DOI: 10.1007%2Fs11694-018-9773-9.
28. Rogozhin V.V., Kuriliuk T.T., and Filippova N.P. 2000. Change in the reaction of the antioxidant system of wheat sprouts after UV-irradiation of seeds. Biofizika 45: 730-736.
29. Salama H.M., Al Watban A.A., and Al-Fughom A.T. 2011. Effect of ultraviolet radiation on chlorophyll, carotenoid, protein and proline contents of some annual desert plants. Saudi Journal of Biological Sciences 18(1): 79-86. DOI: 10.1016/j.sjbs.2010.10.002.
30. Sarghein S.H., Carapetian J., and Khara J. 2008. Effects of UV-radiation on photosynthetic pigments and UV absorbing compounds in Capsicum longum (L.). International Journal of Botany DOI:10.3923/ijb.2008.486.490.
31. Singleton, V.L. and Rossi, J.A. 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158.
32. Stalikas C.D. 2007. Extraction, separation, and detection methods for phenolic acids and flavonoids. Journal of Separation Science 30(18):3268-95. DOI: 10.1002/jssc.200700261.
33. Teramura A.H., and Sullivan J.H. 1994. Effects of terrestrial plants. Photosynthesis Research 39: 463-473. DOI:10.1007/BF00014599.
34. Zhang W.J., and Björn L.O. 'The effect ofultraviolet radiation on the accumulation ofmedicinal compounds in plants'. Fitoterapia80(4): 207-218.DOI: 10.1016/j.fitote.2009.02.006.
35. Zhao G.Q., Ma B.L., and Ren C.Z. 2007. Growth, gas exchange, chlorophyll fluorescence, and ion content of naked oat in response to salinity. Crop Science 47(1): 123-131. DOI:10.2135/cropsci2006.06.0371.