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مجید عزیزی منصور مشرقی فاطمه عروجعلیان ناصر شاه طهماسبی

چکیده

به منظور بررسی برهم‌کنش نانوذرات نقره با اسانس‌های گیاهان دارویی کرفس‌کوهی و کلپوره از نظر اثرات ضدباکتریایی، آزمایشی براساس محاسبه حداقل غلظت بازدارندگی (MIC) ترکیبی و تعیین شاخص FIC یا غلظت بازدارنده افتراقی و با روش ساده شده checkboard طراحی و به اجرا درآمد. اسانس گیاهان مورد بررسی به روش تقطیر با آب استخراج گردید. در این تحقیق باکتری‌های آلوده کننده مواد غذایی رایج مانند Staphylococcus aureus، Bacillus cereus Listeria monocytogenes، Escherichia coli O157:H7 و Salmonella enterica و Pseudomonas aureogenosa مورد استفاده قرار گرفتند. نتایج این تحقیق نشان داد که خاصیت ضدباکتریایی اسانس کلپوره قویتر(MIC بین 16/0 تا 25/1 میلی‌گرم در میلی‌لیتر) از اسانس کرفس‌کوهی (MIC بین 3/0 تا 5/2 میلی‌گرم در میلی لیتر) بود. خاصیت ضدباکتریایی نانوذرات نقره نیز (MIC بین 006/0 تا 025/0 میلی‌گرم در میلی‌لیتر) بسیار قابل توجه بود. محاسبه غلظت بازدارنده افتراقی (شاخص FIC) حاکی از وجود اثرات هم افزایی بین نانوذرات نقره و اسانس هر دو گیاه بود که بستگی به نوع پاتوژن مورد بررسی داشت. در نتیجه‌ی کاربرد توام نانوذرات نقره و اسانس این دو گیاه بویژه اسانس کلپوره با غلظت کمتر اسانس خاصیت بازدارندگی قابل توجهی بدست آمد. در هیچکدام از تیمارها برهم‌کنش آنتاگونیستی بین نانوذرات نقره و اسانس این دو گیاه مشاهده نشد.

جزئیات مقاله

مراجع
1-Burt S., 2004. Essential oils: Their antibacterial properties and potential applications in foods- a review. International Journal of Food Microbiology, 94: 223-253.
2-Chifiriuc C., Grumezescu V., Grumezescu A.M., Saviuc C., Lazăr V., and Andronescu E. 2012. Hybrid magnetite nanoparticles/Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity. Nanoscale Research Letters, 7:209-216.
3-Cho K., Park J., Osaka T., and Park S., 2005. The study of antimicrobial activity and preservative effects of nanosilver ingredient, Electrochim Acta, 51: 956–960.
4-Fayaz A. M., Balaji K., Girilal M., Yadav R., Kalaichelvan P. Th., and Venketesan R. 2010. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine: Nanotechnology, Biology and Medicine, 6: 103–109.
5-Fondevila M. 2010. Potential Use of Silver Nanoparticles as an Additive in Animal Feeding, Silver Nanoparticles, David Pozo Perez (Ed.), ISBN: 978-953-307-028-5, InTech, Available from:http://www.intechopen.com/books/silver-nanoparticles/potential-use-of-silver-nanoparticles-as-an-additive-inanimal-feeding.
6-Friedman M., Henika P. R., and Mandrell R. E. (2002). Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenese, and Salmonella enterica. Journal of Food Protection, 65, 1545–1560.
7-Gachkar L., Yadegari D., Rezaei M. R., Taghizadeh M., Alipoor Astaneh Sh., and Rasooli I., 2007. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chemistry, 102, 898-904.
8-Holly R. A., and Patel D. (2005). Improvement in shelf life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Chemistry, 22, 273–292.
9-Kalemba D., and Kunicka A. 2003. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10: 813–829.
10-Kim J. S., E. Kuk et al. 2007. Antimicrobial effects of silver nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 3(1): 95-101.
11-Kordali S., Kotan R., Mavi A., Cakir A., Ala A., and Yildirim A., 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry, 53: 9452–9458.
12-Kotzé M. and Eloff J. N., 2002. Extraction of antibacterial compound from Combretum microphyllum (Combretaceae). South African Journal of Botany, 68: 62–67.
13-Lkhagvajav N., Yasa I., Çelik E., Koizhaiganova M, and Sari Ö. 2011. Antimicrobial Activity of Colloidal Silver Nanoparticles Prepared by Sol-Gel Methods, Digest Journal of Nanomaterials and Biostructures, 6(1): 149 – 154.
14-Lok C., 2006. Proteomic analysis of the mode of antibacterial action of silver nanoparticles, Journal of Proteome Research, 5: 916–924.
15-Mahboubi M., and Ghazian Bidgoli F., (2010). In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine, 17(10):771-774.
16- Martinez-Castanon G. A. Nino-Martınez N., Martınez-Gutierrez F., Martınez-Mendoza J. R. and Ruiz F. 2008. Synthesis and antibacterial activity of silver nanoparticles with different sizes. Journal of Nanoparticle Research, 10:1343–1348.
17-Morones J.R., Elechiguerra J.L., Camacho A, Holt K., Kouri J.B., Ramirez J.T., and Yacaman M.J., 2005. The bactericidal effect of silver nanoparticles, Nanotechnology, 16: 2346–2353.
18-Mozaffarian V. A Dictionary of Iranian Plant Names. Farhange Moaser: Tehran. 2004. p671
19-Oroojalian F., Kasra-Kermanshahi R., Azizi M. and Bassami M.R. 2010. Phytochemical composition of the essential oils from three Apiaceae species and their antibacterial effects on food-borne pathogens. Food Chemistry, 120:766-770.
20-Pillai S.K., Moellering C. and Elipoulos G.M., 2005. Antimicrobial combinations. 365-373, In: V. Lorian, (ed.), Antibiotic in laboratory medicine, Lippincott Williams & Wilkins, Philadelphia, 922p.
21-Rai M., Yadav A., and Gade A. 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27: 76–83.
22-Romano C. S., Abadi K., Repetto V., Vojnov A., A. and Moreno S. 2009. Synergistic antioxidant and antibacterial activity of rosemary plus butylated derivatives. Food Chemistry, 115: 456-461.
23-Sandri I. G., Zacaria J., Fracaro F., Delamare A. P. L., and Echeverrigaray S. (2007). Antimicrobial activity of the essential oils of Brazilian species of the genus Culina against foodborne pathogens and spoiling bacteria. Food Chemistry, 103, 823–828.
24-Sarkar S., Jana A.D., Samanta S.K., and Mostafa G., 2007. Facile synthesis of silver nanoparticles with highly efficient anti-microbial property. Polyhedron, 26: 4419–4426.
25-Saviuc C., Grumezescu AM., Oprea E., Radulescu V., Dascalu L., Chifiriuc MC., Bucur M., Banu O., and Lazar V. 2011a. Antifungal activity of some vegetal extracts on Candida biofilms developed on inert substratum. Biointerface Research Applied Chemistry, 1:15-23.
26-Saviuc C., Grumezescu AM., Holban A., Bleotu C., Chifiriuc C., Balaure P., and Lazar V. 2011b. Phenotypical studies of raw and nanosystem embedded Eugenia carryophyllata buds essential oil antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus strains. Biointerface Research Applied Chemistry, 1:111-118.
27-Tepe B., Donmez E., Unlu M., Candan F., Daferera D., and Vardar-Unlu G., 2004. Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (montbret et aucher ex benth.) and Salvia multicaulis (vahl). Food Chemistry, 84: 519–525.
28-Ulte A., Bennik M. H. J., and Moezelaar R. 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food pathogen Bacillus cereus. Applied and Environmental Microbiology, 68, 1561–1568.
29-Vankar P. S. and Shukla D., 2012. Biosynthesis of silver nanoparticles using lemon leaves extract and its application for antimicrobial finish on fabric. Applied Nanoscience, 2:163–168.
30-Wei D., Sun W., Qian W., Ye Y., and Mac X. 2009. The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydrate Research, 344: 2375–2382.
31-Zargari A. 1988. Medicinal Plants (Vol. 2). Tehran, Iran: Tehran University Publications.
ارجاع به مقاله
عزیزی م., مشرقی م., عروجعلیان ف., & شاه طهماسبی ن. (2016). اثرات ضد باکتریایی ترکیبی اسانس کرفس کوهی) Kelussia odoratissima Mozaffarian) و کلپوره (L. Teucrium polium) با نانوذرات سنتزی نقره علیه باکتری‌های غذا زاد. علوم باغبانی, 30(2), 270-279. https://doi.org/10.22067/jhorts4.v30i2.40634
نوع مقاله
علمی - پژوهشی

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