Document Type : Research Article

Authors

Lahijan Branch, Islamic Azad University, Lahijan, Iran

Abstract

Introduction: Zamiifolia is a perennial ornamental plant and is one of the most important medicinal plants of the Aracea family. The origin of this evergreen, low-anticipated plant is East Africa. Zamiifolia spreads through the leaves and split rhizomes, which is very time-consuming. The traditional Zamiifolia proliferation method have been done by dividing rhizomes and leaf cuttings, but the production efficiency is low due to the slow growth of the plant, tubers and roots.  In addition, due to the warm and humid environment, reproduction is limited to summer season. Due to the traditional reproductive problems in this plant, tissue culture or microbial culture is the best way to replicate rapidly and to achieve a large number of plants with the same genetic structure, as well as the elimination of diseases in the short term and reducing the costs. The aim of this study was to compare different microorganisms in terms of calogenesis and regeneration, as well as to determine the optimum culture medium for Zamiifolia tissue culture.
Materials and Methods: In this study, the explants prepared for the first experiment, including rhizome and petiole and the explants for the second experiment were the leaeaves and shoots. In the first experiment, rhizome and petiole were cultured in three replications in ½ MS medium containing BA (0, 2, 4 mg / L) and 2,4-D (0,1,2 mg / L) in combination with vitamins, 30 g/l sucrose, 5 g / L agar and adjusted to 5.8 PH. The cultivars were cultured for the callus induction under temperature of 27-25 ° C and light conditions of 16 hours light and 8 hours darkness. After 5 weeks, the percentage of callus and fresh callus weight were measured. The callus generated from rhizome and petiole in three replicates on ½ MS medium containing BA (0, 1, 2 mg / L) and 2,4-D (0, 0.5, 1 mg / l) for shoots and after the observation of branches and leaf buds were grafted on to ½ MS medium containing BA (0, 1, 2 mg / L) and NAA (0, 0.5, 1 mg / L) for rooting. Traits such as time to shoot elongation were recorded at regeneration stage, and after 5 weeks, shoot length and the number of leaves were measured. The time to rootstock was also recorded. In the second experiment leaf and shoot explants were cultured in ½ MS medium containing BA (0, 2, 4 mg / l) and NAA (0, 0.5, 1 mg / L) in combination with vitamins, 30 g/l sucrose, 5 g / L agar and PH adjusted to 5.8. The cultivars were cultured for the callus induction under temperature 27-25 °C and light conditions of 16 hours light and 8 hours darkness. The time to reach the callus was recorded and after 5 weeks, the percentage of callus and fresh callus weight were measured. The calli generated from the leaves and shoots were cultures on ½ MS medium containing BA (0, 1, 2 m g / L) and NAA (0, 0.5, 1 mg / L) for shoots and after observation of branch and leaves were transplanted to the ½ MS medium containing BA (0, 1, 2 mg / L) and NAA (0, 1, 2 mg /L) for rooting. The traits such as time to shoot elongation were recorded at the regeneration stage, After 5 weeks, the shoot length and the number of leaves were measured.The time to rootstock was also recorded.
Results and Discussion: The results of the first experiment showed that the effect of the rhizome and petiole type on the callus formation was significant at 1% level. So that the rhizome showed greater ability to callogenesis. The results of the second experiment showed that the effect of the type leaf and shoot on the callus formation was significant at 1% level. So that the leaf showed greater ability to callogenesis. The highest percentage of callosing (94.5%), the shortest time to reach the callus (14 days) and the highest callus weight (1.1 g) in culture medium with 2 mg / l BA and 1 mg / l hormones NAA was observed in leaf samples from the second experiment. The best treatment in the shoot elongation stage, which included the shortest time to shoot formation (10.5 days), the longest shoot length (4.10 cm), and the highest leaf number (8 leaves) in the leaf extract with hormonal concentrations of 2 mg / 0 mg / L NAA was observed from the second experiment. In the rooting stage, the best treatment for petiole extracts with hormonal concentrations was 1 mg / l BA and 0.5 mg / l NAA with 14 days to rooting from the first experiment.
Conclusion: In this study, explants and various concentrations of growth regulators had significant effect on the response to callus induction in Zamiifolia. In the first experiment, the rhizome and in the second experiment the leaf showed a better reaction to callus induction. According to this research, it can be suggested that the treatments applied in both experiments should be applied on all four leaves, petiole, rhizome and shoot samples, and the best culture type and the best culture medium for the cultivation of Zamiifolia plant tissue should be determined in subsequent studies.

Keywords

1- Bejoy M., Sumitha V., and Anish N.P. 2008. Foliar regeneration in Anthurium andraeanum Hort.cv.Agnihothri. Biotechnology 71 (1): 134-138.
2- Chen J., Henny R.J., and McConnell D.B. 2002. Development of new foliage plant cultivars. In: J.J. Janik and A. Whipkey (Eds), Trends in New Crops and New Uses, ASHS Press, Alexandria, Pp. 466-472.
3- Chen J.J., and Henny R.J. 2003. ZZ: A unique tropical ornamental foliage plant. HortTechnology 13 (3): 458-462.
4- Feng C.T., Ho W.C., and Chao Y.C. 2006. Basal petiole rot and plant kill of Zamioculcas zamiifolia caused by Phytophthora nicotianae. Plant Disease 90: 1107-1109.
5- GU L., Yang B., Zhang S., and Yang X. 2006.Tissue culture of Zamioculcas zamiifolia (loddiges) angler. Chinese Journal of Tropical Agriculture 32: 19-25.
6- Harrison M. 2009. The Incredible ZZ plant (Zamioculcas zamiifolia). Available from www.davesgarden.com. Accessed on 14 August 2012.
7- Lin W., Tao J., Li Q., Li W., and Huang L. 2005. Study on Rapid Propagation Techniques of Zamioculcas zamiifolia. Chinese Agricultural Science Bulletin 44: 86-93.
8- Lopez R.G., Blanchard M.G. 2007. ZZ plant is an excellent choice for tough indoor use - Zamioculcas zamiifolia survive most interior environments. Greenhouse Management and Production 27:50-56.
9- Lopez R.G., Blanchard M.G., and Runkle E.S. 2009. Propagation and production of Zamioculcas zamiifolia. Acta Horticulturae 813: 559−564.
10- Ni K. 2015. Zamioculcas zamiifolia plant tissue culture method. Anhui Agricultural Science Bulletin 10(6): 56-63.
11- Papafotiou M., and Martini A. 2009. Effect of position and orientation of leaflet explants with respect to plant growth regulators on micro propagation of Zamioculcas zamiifolia Engl. (ZZ). Scientia Horticulturae 120: 115-120.
12- Pan X.F., Wang J.H., and Fu Q.M. 2007. Study on Rapid Vitro Propagation of Zamioculcas Zamiifolia. Natural Science Journal of Hainan University 23: 105-113.
13- Reid M.S., and Cevallos J.C. 2009. Postharvest biology and technology for new floricultural crops. Acta Horticulture 813: 209-216.
14- Seneviratne K.A.C.N., Daundasekera W.A.M., and Kulasooriya S.A. 2013. Development of rapid propagation methods and a miniature plant for export-oriented foliage, Zamioculcas zamiifolia. Ceylon Journal of Science (Bio Sci) 42(1): 55-62.
15- Shi H., and Liang P. 2003. Plantlet Regeneration from Leaf Explants of Zamioculcas zamiifolia. Acta Horticulturae Sinica 115: 131-139.
16- Vanize-canton S.D., and Leonhardt K.W. 2009. In vitro callus induction and plantlet regeneration protocol developed for the oryzalin treatment of Zamioculcas zamiifolia. Acta Horticulturae 813(26): 201-208.
17- Xue C.L., Guo J.M., Chen Y.J., and Quan S.Z. 2010. Study on Differentiation and Induction Callus and Embryoid from the Leaves of Zamioculcas zamiifolia Rehd. Modern Agricultural Science and Technology 45: 25-33.
18- Zhou J.H., Zhou Y., Liu X.M., Zhu Z.P., Deng B.G., and Luo N.S. 2005. In vitro Culture and Rapid Propagation of Zamioculcas zamifolia. Acta Agriculturae Jiangxi 89: 28-36
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