نوع مقاله : مقالات پژوهشی

نویسندگان

1 گروه علوم باغبانی و مرکز پژوهشی گیاهان ویژه منطقه، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

3 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه بیرجند، ایران

چکیده

به‌منظور بررسی تأثیر کود مرغی و سولفات پتاسیم بر میزان صفات بیوشیمیایی و عملکرد گیاه شنبلیه، پژوهشی به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در سال زراعی 1401-1400 انجام گردید. تیمارهای آزمایش شامل کود پلیت مرغی در سه سطح (صفر، 1000 و 2000 کیلوگرم در هکتار) به‌صورت کاربرد خاکی و سولفات پتاسیم (سولوپتاس) در چهار سطح (صفر، 1/5، 3 و 5 در هزار) به‌صورت محلول پاشی بودند. نتایج نشان داد که کود مرغی در همه صفات به‌جز میزان قند محلول و فعالیت آنتی‌اکسیدانی، سبب افزایش معنی‌دار صفات بیوشیمیایی و عملکرد دانه شنبلیله شد، به‌طوری‌که تیمار 2000 کیلوگرم در هکتار کود مرغی، بالاترین میزان فنل (10/793 میلی‌گرم بر گرم وزن خشک)، میزان فلاونوئید (4/475 میلی‌گرم بر گرم وزن خشک)، کلروفیل a (2/591 میلی‌گرم بر گرم وزن تر) و کلروفیل b (055/3 میلی‌‌گرم بر گرم وزن تر) و عملکرد دانه (1185/8 کیلوگرم در هکتار) را به خود اختصاص داد. در تأثیر سولفات پتاسیم (سولوپتاس) بر صفات بیوشیمیایی شنبلیله مشاهده شد که همه صفات به‌جز میزان فنل و فعالیت آنتی‌اکسیدانی، همگی در سطح سه در هزار، دارای بالاترین میزان بودند که با سطح پنج در هزار اختلاف معنی‌داری نداشتند. نتایج اثر متقابل تیمارها نشان داد که در تمام صفات به‌جز صفت فعالیت آنتی‌اکسیدانی، بالاترین میزان مصرف هر دو کود، بیشترین میزان صفات بیوشیمیایی و عملکرد دانه را به خود اختصاص دادند، به‌طوری‌که، بیشترین سطوح هر دو تیمار (2000 کیلوگرم در هکتار کود مرغی و سولوپتاس 5 در هزار) سبب افزایش عملکرد دانه، میزان فنل و کلروفیل b نسبت به سطح شاهد شد. بیشترین میزان کلروفیل a نیز مربوط به برهم‌کنش 2000 کیلوگرم در هکتار کود مرغی و سولوپتاس 3 در هزار به‌میزان 3/11 میلی‌گرم بر گرم وزن‌تر بود. در مجموع، در بین سطوح مورد بررسی در دو تیمار، سطح 2000 کیلوگرم در هکتار کود مرغی و 3 در هزار سولوپتاس برای دستیابی به افزایش عملکرد دانه و بهبود صفات بیوشیمیایی برای گیاه شنبلیله پیشنهاد می‌گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the Effect of Chicken Manure and Potassium Sulfate on Yield and Biochemical Traits of Fenugreek Medicinal Plant

نویسندگان [English]

  • M.H. Aminifard 1
  • S. Nadafan 2
  • H. Bayat 2
  • M. Jahani 3

1 Department of Horticultural Sciences and Regional Plant Research Center, Faculty of Agriculture, Birjand University, Birjand, Iran

2 Department of Horticultural Sciences, Faculty of Agriculture, Birjand University, Birjand, Iran

3 Department of Plant Protection, Faculty of Agriculture, Birjand University, Iran

چکیده [English]

Introduction
The annual herb Trigonella foenum-graecum L. (Fabaceae) is cultivated worldwide as a semi-arid crop. It is commonly known as Fenugreek and used as both a spice and medicinal plant. Fenugreek is used to treat many ailments due to the presence of various bioactive compounds, like apigenin, luteolin, orientin, quercetin, vitexin, isovitexin, saponins, amino acids, phenols, alkaloids, etc. Combining chemical and organic fertilizers offers a sustainable approach to nutrient management. This integrated strategy enhances the effectiveness of chemical fertilizers, fosters improved soil health, and minimizes nutrient loss from the system.Chicken manure is one of the types of animal manure and a source of organic matter to strengthen all types of soil. In addition to having nutrients, it is one of the cheap fertilizers compared to common fertilizers in the production of crops, and it is richer in nitrogen than other animal fertilizers. Potassium is a critical element for plant growth and development, playing a vital physiological role in plant health and resilience against biotic and abiotic stresses.
 
Materials and Methods
To investigate the effect of chicken manure and potassium sulfate on yield and biochemical traits of the fenugreek medicinal plant, factorial research was conducted in the form of randomized complete block design in three replications in the crop year 2021-2022. Experimental treatments included chicken manure at three levels (0, 1000, and 2000 kg.ha-1) and potassium sulfate at four levels (0, 1.5, 3, and 5 per thousand). To determine the seed yield after full ripening, the seeds were collected from one plant in each plot, weighed, and recorded. Arnon's method was used to measure the pigments in the leaves at the full flowering stage of the plant. To measure the amount of total phenolic compounds in the leaf using the Folin Cicalto method, antioxidant using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, total sugar using anthrone method, total flavonoid using aluminum chloride reagent method was used in the full flowering stage of the plant.
 
Results and Discussion
This experiment revealed that chicken manure significantly boosted crop yield and improved most biochemical traits, with the exception of sugar and antioxidant content.The amount of antioxidant activity decreased with increasing levels of chicken manure and soluble sugar was not affected by this manure. Treatment of 2000 kg.h-1 chicken manure has the highest phenol with 10.793 mg.g-1 dry weight, flavonoid with 4.475 mg.g-1 dry weight, Chlorophyll a with 2.591 mg.g-1 of fresh weight, Chlorophyll b with 3.055 mg.g-1 of fresh weight, and seed yield with 1185.8 kg.h-1. Regarding the impact of Soluptas on biochemical traits, all except phenol and antioxidant content peaked at a concentration of three parts per thousand. Notably, these peak levels were statistically indistinguishable from those observed at five parts per thousand.Treatment of 5 per thousand Soluptas also increased 28% seed yield, 13% soluble sugar, 20% phenol, 31% flavonoid, and 97% chlorophyll b. In the interaction effect in the treatment, it was observed that the highest amount of the two fertilizers had the highest amount of yield and biochemical traits, except for the antioxidant, whose activity level decreased with the increase of fertilizer levels. The results showed that the highest amount of soluble sugar in fenugreek leaves was related to the level of no chicken fertilization with Soluptas 5 per thousand at the rate of 21.53 mg.g-1 dry weight. The highest levels of both treatments (2000 kg.h-1 of chicken manure and Soluptas 5 per thousand) caused an increase in grain yield (1396 kg.h-1), phenol (12.86 mg.g-1 DW), and chlorophyll b (3.62 mg.g-1 FW) compared to no fertilization (control level). The highest amount of chlorophyll a was related to the interaction of 2000 kg.h-1of chicken manure and Soluptas 3 per thousand at the rate of 3.11 mg.g-1 of fresh weight.
 
Conclusion
Overall, chicken manure exhibited the strongest positive influence on the greatest number of traits among the single treatments. When considering the combined effects, a combination of 2000 parts per thousand chicken manure and 5 parts per thousand Soluptas appears to be optimal for enhancing the fenugreek plant's properties.
 

کلیدواژه‌ها [English]

  • Chlorophyll
  • Organic fertilizer
  • Phenol
  • Soluptas
  • Yield

©2024 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

  • Al-Taai, S.H.H. (2021). The Effect of fertilizer uses on environmental pollution: A review. Review of International Geographical Education Online, 11(5), 3620-3529.
  • Arisha, H., & Bradisi, A. (1999). Effect of mineral fertilizers and organic fertilizers on growth, yield and quality of potato under sandy soil conditions. Zagazig Journal Agriculture Research, 26, 391-405.
  • Ashnavar, M., Bahmanyar, M.A., & Akbarpour, V. (2014). Investigation on the effect of different sources of fertilizers on growth indices and yield of coneflower (Echinacea purpurea) as a medicinal plant. Journal of Agroecology, 6(2), 266-274. (In Persian with English abstract). https://doi.org/10.22067/JAG.V6I2.39510
  • Badr, L., & Fekry, W. (1998). Effect of intercropping and doses of fertilization on growth and productivity of taro and cucumber plants. 1-vegetative growth and chemical constituents of foliage. Zagazig Journal of Agriculture Research, 25, 1087-1101.
  • Birjely, H.M.S., & Al-Atrushy, S.M.M. (2017). Effect of some organic and non-organic fertilizers on some parameters of growth and berries quality of grape cv. Kamali. Kufa Journal for Agricultural Sciences, 9(3), 262-274.
  • Chuah, A.M., Lee, Y.C., Yamaguchi, T., Takamura, H., Yin, L.J., & Matoba, T. (2008). Effect of cooking on the antioxidant properties of coloured peppers. Food Chemistry, 111(1), 20-28. https://doi.org/10.1016/j.foodchem.2008.03.022 
  • Constán-Aguilar, C., Leyva, R., Romero, L., Soriano, T., Blasco, B., & Ruiz, J.M. (2015). The effect of potassium biofortification over yield and nutritional quality of cherry tomato fruits. American Journal of Advanced Food Science Technology, 3(2), 67-93. https://doi.org/10.7726/ajafst.2015.1006 
  • Esmaeilian, Y., & Jalali, A. (2022). Evaluation of quantitative, qualitative, and economic aspects of garlic (Allium sativum) and fenugreek (Trigonella foenum-graceum L.) intercropping under organic and chemical nutrition systems. Iranian Journal of Medicinal and Aromatic Plants Research, 38(1), 133-149. (In Persian with English Summary). https://doi.org/10.22092/ijmapr.2022.354814.3003
  • Fanasca, S., Colla, G., Maiani, G., Venneria, E., Rouphael, Y., Azzini, E., & Saccardo, F. (2006). Changes in antioxidant content of tomato fruits in response to cultivar and nutrient solution composition. Journal of Agricultural Food Chemistry, 54(12), 4319-4325. https://doi.org/10.1021/jf0602572 
  • Fereidooni, M.J., Maghsoudi, E., Mojabghasroldashti, A., & Behzadi, Y. (2018). Investigation the effect of different fertilizing levels from various sources on yield, and grain quality of sweet corn. Journal of Plant Ecophysiology, 10(33), 79-89. (In Persian with English abstract). https://doi.org/20.1001.1.20085958.1397.10.33.8.5
  • Ghahremani, A., Akbari, K., Yousefpour, M., & Ardalani, H. (2014). Effects of nano-potassium and nano-calcium chelated fertilizers on qualitative and quantitative characteristics of Ocimum basilicum. International Journal for Pharmaceutical Research Scholars, 3(12), 325-241.
  • Heakal, M., Modaihsh, A., Mashhady, A., & Metwally, A. (1990). Combined effects of leaching fraction, salinity, and potassium content of waters on growth and water-use efficiency of wheat and barley. Plant Soil, 125(2), 177-184. https://doi.org/10.1007/bf00010655 
  • Houshyarifard, M., & Qaranchik, A. (2009). Effect of source and rate of manures on incidence and severity of important diseases, yield and yield components in cotton (Gossypium hirsutum). Iranian Society of Crops and Plant Breeding Sciences, 11(3), 237-248. (In Persian with English abstract). http://doi.org/20.1001.1.15625540.1388.11.3.4.8
  • Kafi, M.G., Zamani, S., & Ghoraishi, G. (2009). Relative them together. New Phytologist, 14(1), 63-79.
  • Khaldbrin, B., & Islamzadeh, T. (2014). Mineral nutrition of higher plants. Shiraz University. https://doi.org/10.1016/b978-0-12-473542-2.x5000-7 
  • Khalesro, S., & Malekian, H. (2017). Effects of vermicompost and humic acid on morphological traits, yield, essential oil content and component in organic farming of ajwan (Trachyspermum ammi ). Iranian Journal of Medicinal Aromatic Plants Research. 32(6), 968-980. (In Persian with English abstract). https://doi.org/10.22092/ijmapr.2017.109314
  • Kheiry, A., Arghavani, M., & Khastoo, M. (2016). Effects of organic fertilizers application on morphophysiological characteristics of calendula (Calendula officinalis ). Iranian Journal of Medicinal Aromatic Plants Research, 31(6), 1047-1057. (In Persian with English abstract). https://doi.org/10.22092/ijmapr.2016.105893
  • Lawrence, J.R., Ketterings, Q., & Cherney , (2008). Effect of nitrogen application on yield and quality of silage corn after forage legume‐grass. Agronomy Journal, 100(1), 73-79. https://doi.org/10.2134/agronj2007.0071 
  • Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In Methods in Enzymology, 148, 350-382. https://doi.org/10.1016/0076-6879(87)48036-1 
  • Lv, X., Li, T., Wen, X., Liao, Y., & Liu, Y. (2017). Effect of potassium foliage application post-anthesis on grain filling of wheat under drought stress. Field Crops Research, 206, 95-105. https://doi.org/10.1016/j.fcr.2017.02.015 
  • Mahmood, F., Khan ,, Ashraf, U., Shahzad, T., Hussain, S., Shahid, M., Abid, M., & Ullah, S. (2017). Effects of organic and inorganic manures on maize and their residual impact on soil physico-chemical properties. Journal of Soil Science Plant Nutrition, 17(1), 22-32. https://doi.org/10.4067/s0718-95162017005000002 
  • Mocready, R., Guggolz, J., Silviera, V., & Owens, H. (1950). Determination of starch and amylose in vegetables. Application to peas. Analytical Chemistry, 22, 1156-1158. https://doi.org/10.1021/ac60045a016 
  • Naguib, A.E.M. M., El-Baz, F.K., Salama, Z.A., Hanaa, H.A.E.B., Ali, H.F., & Gaafar, A.A. (2012). Enhancement of phenolics, flavonoids and glucosinolates of broccoli (Brassica olaracea, Italica) as antioxidants in response to organic and bio-organic fertilizers. Journal of the Saudi Society of Agricultural Sciences, 11(2), 135-142. https://doi.org/10.1016/j.jssas.2012.03.001 
  • Naser, A.A. (2017). Study of response Trigonella foenum-graecum to spraying with high potassium (Miller) and high phosphor (Agroleaf). Tikrit Journal of Pure Science, 22(9), 6-10. https://doi.org/10.25130/tjps.v22i9.866 
  • Neisani, S., Fallah, S., & Raiesi, F. (2011). The effect of poultry manure and urea on agronomic characters of forage Maize under drought stress conditions. Journal of Agricultural Science and Sustainable Production, 21(4), 63-74. (In Persian with English abstract)
  • Nguyen, P.M., Kwee, E.M., & Niemeyer, E.D. (2010). Potassium rate alters the antioxidant capacity and phenolic concentration of basil (Ocimum basilicum) leaves. Food Chemistry, 123(4), 1235-1241. https://doi.org/10.1016/j.foodchem.2010.05.092 
  • Nojavan, S., Naseri, L., & Hasanpour, H. (2016). The effect of foliar spraying of potassium sulfate and zinc sulfate on some physical and chemical characteristics of red quince variety grapes. Bi-quarterly Journal of Plant Production Technology, 8(2), 195-213. (In Persian with English abstract). https://doi.org/10.22084/PPT.2016.1865
  • Oosterhuis, D.M., Loka, D.A., & Raper, T.B. (2013). Potassium and stress alleviation: Physiological functions and management of cotton. Journal of Plant Nutrition Soil Science, 176(3), 331-343. https://doi.org/10.1002/jpln.201200414 
  • Prasad, D., Singh, R., & Singh, A. (2010). Management of sheath blight of rice with integrated nutrients. Indian Phytopathology, 63(1), 11.
  • Rostaei, M., & Fallah, S. (2016). Assessment of canopy characteristics and essential oil yeild of fenugreek and black cumin in intercropping under application of organic and chemical ferilizer. Journal of Agricultural Science and Sustainable Production, 25(4), 1-23. (In Persian with English abstract)
  • Salama, Z.A., El Baz, F.K., Gaafar, A.A., & Zaki, M.F. (2015). Antioxidant activities of phenolics, flavonoids and vitamin C in two cultivars of fennel (Foeniculum vulgare) in responses to organic and bio-organic fertilizers. Journal of the Saudi Society of Agricultural Sciences, 14(1), 91-99. https://doi.org/10.1016/j.jssas.2013.10.004 
  • Salehi, A., Fallah, S., Abasi Sourki, A., & Tadayon, M.R. (2017). Evaluation of yield and yield components of fenugreek (Trigonella foenum-graecum) and buckwheat (Fagopyrum esculentum Moench) under organic and chemical fertilizers Iranian Journal of Medicinal and Aromatic Plants Research, 33(2), 338-352. (In Persian with English abstract). https://doi.org/10.22092/ijmapr.2017.106802.1804
  • Samavat, S., Pazki, A.R., Laden Moghadam, A.R., & Samawat, S. (2017). Applied principles of organic materials in agriculture. Publications of Islamic Azad University, Garmsar branch. (In Persian)
  • Sanei, S.J., & Razavi, S.I. (2018). Effects of potassium nitrate on growth and photosynthetic pigments of basil under Colletotrichum gloeosporioides Horticultural Plants Nutrition, 1(1), 49-58. (In Persian with English abstract). https://doi.org/10.22070/HPN.2018.454
  • Scharf, P.C., Kitchen, N.R., Sudduth, K.A., Davis, J.G., Hubbard, V.C., & Lory, J.A. (2005). Field‐scale variability in optimal nitrogen fertilizer rate for corn. Agronomy Journal, 97(2), 452-461. https://doi.org/10.2134/agronj2005.0452 
  • Serra Bonvehí, J., Soliva Torrentó, M., & Centelles Lorente, E. (2001). Evaluation of polyphenolic and flavonoid compounds in honeybee-collected pollen produced in Spain. Journal of Agricultural Food Chemistry, 49(4), 1848-1853. https://doi.org/10.1021/jf0012300 
  • Soares, A.G., Trugo, L.C., Botrel, N., & da Silva Souza, L.F. (2005). Reduction of internal browning of pineapple fruit (Ananas comusus ) by preharvest soil application of potassium. Postharvest Biology Technology, 35(2), 201-207. https://doi.org/10.1016/j.postharvbio.2004.07.005 
  • Tabatabaei, S.J. (2018). Principles of plant mineral nutrition. First edition, author's publications. Tabriz, Iran.
  • Taber, H., Perkins-Veazie, P., Li, S., White, W., Rodermel, S., & Xu, Y. (2008). Enhancement of tomato fruit lycopene by potassium is cultivar dependent. HortScience, 43(1), 159-165. https://doi.org/10.21273/hortsci.43.1.159 
  • Tavallali, V., Esmaili, S., & Karimi, S. (2018). Nitrogen and potassium requirements of tomato plants for the optimization of fruit quality and antioxidative capacity during storage. Journal of Food Measurement Characterization, 12(2), 755-762. https://doi.org/10.1007/s11694-017-9689-9 
  • Tehranifar, A., & Tabar, S.M. (2009). Foliar application of potassium and boron during pomegranate (Punica granatum) fruit development can improve fruit quality. Horticulture Environment Biotechnology, 50(3), 191-196.
  • Turkmen, N., Sari, F., & Velioglu, Y.S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry, 93(4), 713-718. https://doi.org/10.1016/j.foodchem.2004.12.038 
  • Vojodi Mehrabani, l. (2020). The effects of organic and chemical fertilizers on some morphological and physiological traits of Petroselinum crispum Journal of Plant Ecophysiology, 12(2), 86-96. (In Persian with English abstract). http://doi.org/20.1001.1.20085958.1399.12.41.8.5
  • Wani, S.A., & Kumar, P. (2018). Fenugreek: A review on its nutraceutical properties and utilization in various food products. Journal of the Saudi Society of Agricultural Sciences, 17, 97–106. https://doi.org/10.1016/j.jssas.2016.01.007 
  • Yazdani Biuki, R., Rezvani Moghaddam, P., Khazaie, H.R., & Astaraei, A. (2010). Qualitative and qualitative characteristics of milk thistle (Silybum marianum) in response to organic, biological and chemical fertilizers Journal of Agroecology, 2(4), 548-555. (In Persian with English abstract). http://doi.org/0.22067/JAG.V2I4.8783
  • Zahoor, R., Zhao, W., Dong, H., Snider, J.L., Abid, M., Iqbal, B., & Zhou, Z. (2017). Potassium improves photosynthetic tolerance to and recovery from episodic drought stress in functional leaves of cotton (Gossypium hirsutum). Plant Physiology Biochemistry, 119, 21-32. https://doi.org/10.1016/j.plaphy.2017.08.011 
  • Zgallaï, H., Steppe, K., & Lemeur, R.J.J.O.I.P.B. (2006). Effects of different levels of water stress on leaf water potential, stomatal resistance, protein and chlorophyll content and certain anti‐oxidative enzymes in tomato plants. Journal of Integrative Plant Biology, 48(6), 679-685. https://doi.org/10.1111/j.1744-7909.2006.00272.x 
  • Zhao, X., Liu, Y., Liu, X., & Jiang, J. (2018). Comparative transcriptome profiling of two tomato genotypes in response to potassium-deficiency stress. International Journal of Molecular Sciences, 19(8), 2402. https://doi.org/10.3390/ijms19082402 
  • Zörb, C., Senbayram, M., & Peiter, E. (2014). Potassium in agriculture–status and perspectives. Journal of plant physiology, 171(9), 656-669. https://doi.org/10.1016/j.jplph.2013.08.008 

 

 

 

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