Evaluation of the Effects of Different Basal Medium and Plant Growth Regulators on in Vitro Growth of Hazelnut

Document Type : Research Article

Authors

• Parisa Daryani ¹
• Nasser Zare ¹
• Esmaeil Chamani ¹
• P. Sheikhzade Mosaddegh ²
• Davoud Javadi mojaddad ³

¹ University of Mohaghegh Ardabili

² Mohaghegh Ardabili University

³ Gilan Agricultural and Natural Resources Research Center

10.22067/jhorts4.v30i3.37852

Abstract

In this research, the effects of different basal medium and plant growth regulators on in vitro establishment and growth of hazelnut were investigated. For this, the spring apical and auxiliary buds of cv. Fertile were sterilized and cultured on NRM, MS and 1/2MS basal media containing 0.01 mg/l IBA and different levels of BAP. The results indicated that percentage of explant growth (shooting), number of leaves per explant and shoot length influenced significantly by basal media and concentration of plant growth regulator. Based on orthogonal contrasts analysis, although the highest percentage of shooting was obtained on MS medium, shoot length of explants cultured on NRM basal medium were significantly higher than those of MS and 1/2MS. The best growth response of explants in establishment stage (50% shooting, 5.33 leaves per shoot and 1.6 cm shoot length) were obtained with NRM medium supplemented with 0.01 mg/l IBA and 1 mg/l BAP. Shoots derived from establishment stage were cut to single-node explants and transferred on NRM medium supplemented with 0.05 mg/l IBA and different levels of BAP and TDZ. The highest percentage of explants growth with lowest callgenesis and explnt browning were obtained on NRM medium containing 0.05 mg/l IBA and 5 mg/l BAP.

Keywords

• Apical and auxiliary buds
• In vitro micropropagation
• Corylus avellana
• Plant tissue culture

20.1001.1.20084730.1395.30.3.19.8

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References

1- Anderson W.C. 1983. Micropropagation of filbert (Corylus avellana). Proceedings of the International Plant Propagators Society, 33: 132-139.

2- Bhojwani S.S., and Razdan M.K. 1996. Clonal Propagation. p. 483-536. In S.S. Bhojwani and M.K. Razdan (ed.) Plant Tissue Culture: Theory and Practice. A Revised Edition. Elsevier, Amsterdam.

3- Damiano C., Catenaro E., Ginovinazzi A., and Caboni E. 2005. Micropropagation of hazelnut (corylus avellana). Acta Horticulture, 686:226-227.

4- Diaz-Sala C., Rey M., and Rodriguez R. 1994. Temporary modification of adult filbert proliferation capacity by sequential subcultures: intensive pruning as a pre-treatment for in vitro reinvigoration. Journal of Horticultural Science and Biotechnology, 69:673-678.

5- Driver J.A., and Kuniyuki A.H. 1984. In vitro propagation of Paradox walnut Juglans hindsii × Juglans regia rootstock. Hort Science, 19:507-509.

6- Fatima A., and Jabeen Khan S. 2010. Some factors affecting the in vitro growth of Stevia rebaudiana Bertoni. Iranian Journal of Plant Physiology, 1:61-68.

7- Machakova I., Zazimalova E., and George E.F. 2008. Plant growth regulators I: Introduction; auxins, their analogues and inhibitors. p. 175-204. In E.F. George, A.H. Micheal, D.K. Greet-Jan (eds.) Plant propagation by tissue culture. V.1, The background, 3rd ed., Springer.

8- Mehlenbacher S.A. 1994. Genetic improvement of the hazelnut. Acta Horticulturae, 351:23-37.

9- Murashige T., and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physioloay, 15:473-497.

10- Nuri Nas M .2003. Inclusion of polyamines in the medium improves shoot elongation in hazelnut (Corylus avellana L.) micropropagation. Turkish Journal of Agriculture and Forestry, 28:189-194.

11- Nuri Nas M., and Read P.E. 2000. Micropropagation of hybrid hazelnut: medium composition, physical state and iron source affect shoot morphogenesis, multiplication and explant vitality. Acta Horticulturae, 556:251–258.

12- Nuri Nas M., and Read, P.E. 2003. A hypothesis for the development of a defined tissue culture medium of higher plants and micropropagation of hazelnuts. Scientia Horticulturae, 101:189–200.

13- Pati P.K., Rath S.P., Sharma M., Sood A., and Ahuja P.S. 2005. In vitro propagation of rose a review. Biotechnology Advances, 24:94-114.

14- Stahl L. 2007. Third crop options hybrid hazelnuts. Rural Advantage, 507:238-5449.

15- Thompson M.M., Lagerstedt H.B., and Mehlenbacher A. 1996. Hazelnut. p. 125-184. In j. janick and j. Moore (ed.) Fruit Breeding, Volume 3. Nuts. John Wiley and Sons, New York.

16- Yu X.L., and Reed B.M. 1993. Improved shoot multiplication of mature Hazelnut (Corylus avellana L.) in vitro using glucose as a carbon source. Plant Cell Reports, 12:256-259.

17- Zhao Y., Li X.L., Amarasinghe A.A.Y., and Yang Y.S. 2009. Influence of physiological state of fresh and dry explants on in vitro culture response in rice (Oryza sativa L.). Journal of Agricultural Sciences, 4:1-6.