Effects of Salicylic acid and Humic acid on Vegetative Indices of Periwinkle (Catharanthus roseusL.)

Document Type : Research Article

Authors

• Esmaeil Chamani
• Mohammad Bonyadi
• Alireza Ghanbari

University of Mohaghegh Ardabili

https://doi.org/10.22067/jhorts4.v29i4.33521

Abstract

Introduction: Vinca flower (Catharanthus roseus L.) is one of the most important medicinal plants of Apocynaceae (31, 27). Tropical plant native to a height of 30 to 35 centimeters (9) and a perennial shrub which is grown in cold areas for one year (27).One of the plants in the world today as a medicinal plant used the periwinkle plant. Among the 130 indole – terpenoids alkaloids which have been identified in the plant periwinkle vinca alkaloids vincristine and vinblastin are the most important component is used to treat a variety of cancers. Including therapies that are used for a variety of cancer, chemotherapy to help Vinca alkaloids collection (including vincristine and…). Vinblastin as effective member of this category, due to the low percentage of venom and effects at very low doses, is widely used today. These materials are generally formed as inhibitors of mitotic spindle in dividing cells have been identified. Vinblastin with these structural changes in connection kinotokor - microtubules and centrosomes in a dividing cell, the mitotic spindle stop (45).Salicylic acid belongs to a group of phenolic compounds found in plants, and today is widely regarded as a hormone-like substance. These classes of compounds act as growth regulators. Humic substances are natural organic compounds that contain 50 to 90% of organic matter, peat, charcoal, rotten food and non-living organic materials are aquatic and terrestrial ecosystems (2).
Materials and Methods: In this experiment, vinca F2 seeds in the mixed 4: 1 perlite and peat moss to the planting trays were sown. The seedlings at the 6-leaf stage were transfered to the main pot (pot height 30 and 25 cm diameter) The pots bed soil mix consisting of 2 parts soil to one part sand and one part peat moss (v / v) were used and after the establishment of seedlings in pots every two weeks with. Salicylic acid and humic acid concentrations 0 (control), 10, 100, 500 and 1000 mg were treated as a foliar spray. Salicylic acid and humic acid used in the Merck has the solutions according to plant size in proper volume has been prepared and will be sprayed on aerial spraying. According to the bootblack periwinkle flowers and leaves to prevent leaf burn and create the solution at one point, for every cc100 solution, two drops of Tween 20 was added to the solution, then spray on the leaves and leaf fire does not spread. The experiment was conducted in a completely randomized design with 10 replicates at the end of the results by the SAS software analysis and comparison of means by Duncan's multiple range tests was performed.
Results and Discussion: According to the results of the data analysis of different treatments significant impact on the level of 1% of the height, number of leaves, chlorophyll, stomatal conductance, pods and stems of the side. Also, the tally was significant at 5%.According to the results of the data analysis of different treatments on stem diameter had no significant effect. Results of comparing the average of the data showed that treatment with 10 and 500 mg/l of salicylic acid per liter respectively in the first and second measurement and control showed lowest height. Treatment of 100 mg/l of humic acid maximum height was measured in two stages. The results of the comparison showed that an average of 500 mg/l of salicylic acid in a two-step measurement and control had the lowest number of leaves. Treatment with 10 mg/l in the first stage of the operation (L1) and treated with 100 mg/l of humic acid in the second vector data (L2) had the highest number of leaves. Treatment with 10 mg/l of salicylic acid and 100 mg/l of humic acid had the highest chlorophyll. The treatment of 10 mg/l of salicylic acid and 100 mg/l of humic acid had the highest stomatal conductance. The results of the comparison average showed that the 500 mg/l of salicylic acid and humic acid had the greatest impact on the number of flowers. As well as 500 mg/l salicylic acid and humic acid had the greatest impact on the number of pods. The results showed that treatment with 1000 mg/l salicylic acid and humic acid had the greatest effect on stem diameter.
Conclusion: The results of this study indicated that low concentrations of salicylic acid increased plant height, the number of leaves, chlorophyll content and stomatal conductance, which can increase plant resistance against unfavorable environmental conditions.
As a result, the plants treated with salicylic acid can be increased two driven in adverse environmental conditions. The treatment of humic acid by increasing the rate of photosynthesis and increases the amount of material available for plant growth. This increase can accelerate the growth of the main branch and side periwinkle plant medicinal plants and enhances the appearance of the plant.

Keywords

• Alkaloid
• Chlorophyll
• Stomata Conductivity

20.1001.1.20084730.1394.29.4.15.9

References

1- Omidbeigi R., 2007. Production and processing of medicinal plants. Ed2. Astane Ghodse Razavi,Mashad, P347 (in Persian).

2- Baiat H., Nemati S. H., Tehranifar A., Vahdati N. and Salahvarzi A. 2012. Effect of Salicylic acid on growth and ornamental Properties of Petunia hybrida under salinity stress. Journal of Science and Technology of Greenhouse Culture. 3(11):43-50 (in Persian with English abstract).

3- Sabzevari S., Khazaei H R. 2009. Effect of foliar application of humic acid on growth and yiel properties of Triticum aestivum L. Cv. Pishtaz. Agroecology journal.1(2):53-63.

4- Shekari F., Pakmehr, A., Saba J., Vazayefi M., Zangani A., 2011. Effect of Salicylic Acid Seed Priming on Some morphological trats of Vingna unguiculata L. under water deficiency stress. Journal of Crop Ecophysiology. 4(1):1-22 (in Persian with English abstract).

5- Ghasemi Ghahsareh M. and Kafi M. 2004. Practical and Sientific Floriculture, Golban, Tehran. P 335 (in Persian).

6- Koocheki A., Tabrizi L. and Ghorbani R. 2006. Effect of biofertilizers on agronomic and quality criteria of Hyssop (Hyssopus officinalis). Journal of Iranian Field Crop Research.6(8):127-128 (in Persian with English abstract).

7- Majd A., Maddah S.M., Fallahian F., Sabaghpour S.H. and Chalabian F.2005. Comparative study of the effect of salicylic acid on yield, yield components and resistance of two susceptible and resistant chickpea cultivars to Ascochyta rabiei. Iranian journal of Biology. 19(3):314-324 (in Persian with English abstract).

8- Mardani H., Azizi M., 2011. Effects of Salicylic acid Application on Morphological and Physiological Characteristics of Cucumber Seedling (Cucumis sativus cv. Super Dominus) under drought Stress. Journal of horticulture science. 25(3):320-326 (in Persian with English abstract).

9- Mehrabian N., Arvin M J., Khajoei Nejad Gh R., and Maghsoudi K. 2011. Effect of Salicylic Acid on Growth and Forage and Grain Yield of Maize under Drought Stress in Field Conditions. Seed and Plant Improvment Journal. 27(2):41-55 (in Persian with English abstract).

10- Abreu ME, Munne-Bosch S. 2009. Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana. Journal of Experimental Botany, 60:1261-1271.

11- Cacco G, Attina E, Gelsomino A, Sidari M. 2000. Effect of nitrate and humic substances of different molecular size on kinetic parameters of nitrate uptake in wheat seedlings. Journal. Plant Nutrition. Soil Science, 163: 313-320.

12- Clapp CE, Hayes MHB, Swift RS. 1993. Isolation, fractionation, functionalities, and concepts of structure of soil organic macromolecules, in A J. Beck, K.C. Jones, M.B.H. Hayes, and U. Mingelgrin (eds.), Organic substances in soil and water. Royal Society of Chemistry, Cambridge.

13- David PP, Nelson PV, Sanders DC. 1994. A humic acid improves growth of tomato seedling in solution culture. Journal of Plant Nutrition, 17: 173–184.

14- Delany TP, Uknes S, Vernooij B, Friedrich L, Weymann K, Negrotto D, Gaffney T, Gut-Rella M, Kessmann H, Ward E, Ryals J. 1994. A central role of salicylic acid in plant disease resistance. Science, 266: 1247-125.

15- Delfine S, Tognetti R, Desiderio E, Alvino A. 2005. Effect of foliar application of N and humic acids on growth and yield of durum wheat. Agronomy Sustainable, 25, 183-191.

16- Du YC, Nose A, Wasano K, Ushida Y. 1998. Responses to water stress of enzyme activities and metabolite levels in relation to sucrose and starch synthesis, the Calvin cycle and the C4 pathway in sugarcane (Saccharum sp). Australian Journal of Plant Physiology, 25:253 – 260.

17- Eraslan F, Inal A, Gunes A, Alpaslan M. 2007. Impact of exogenous salicylic acid on growth, antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity. Scientia Horticulturae, 113: 120–128.

18- Fernandes-Escobar R, Benlloch M, Barranco D, Duenas A, Guterrez Ganan JA. 1996. Response of olive trees to foliar application of humic substances extracted from leonardite. Scientia Horticulture, 66: 191-200.

19- Galal A. 2012. Improving effect of salicylic acid on the multipurpose tree Ziziphusspina-christi (L.) Willd Tissue Culture. American Journal of Plant Sciences, 3(7) : 947-952.

20- Garcia MG, Busso CA, Polci P, Garcia LN, Echenique V. 2002. Water relation and leaf growth rate of three Agropyron genotypes under water stress. Biology of Cell, 26: 309-317.

21- Gregg. A. Howe. 2010. The rololes of hormones in defense against insect and disease. Plant Hormones springer, 646-680.

22- Gunes A, Inal A , Alpaslan M. 2005. Effects of exogenously applied salicylic acid on the induction of multiple stress tolerance and mineral nutrition in maize (Zea mays L.). Archives Agronomy Soil Science, 51: 687-695.

23- Gutierrez-Coronado MA, Trejo-Lopez C, Larque-Saavedra A. 1998. Effects of salicylic acid on the growth of roots and shoots in soybean. Plant Physiolgy Biochemistry, 36: 563-565.

24- Hussein MM, Balbaa LK, Gaballah MS. 2007. Salicylic acid and salinity effects on growth of maize plants. Research Journal of Agriculture and Biological Sciences, 3: 321-328.

25- Jones CA, Jacobsen JS, Mugaas A. 2004. Effect of humic acid phosphorus availability and spring wheat yield. Fact. Fertilizer. 32.

26- Kang G and Wang Ch. 2003. Environmental and Experimental Botany, 50:9-15.

27- Karakurt Y, Unlu Ha, Padem H. 2008. The influence of foliar and soil fertilization humic acid on yield and quality of pepper. Plant Soil Science, 233- 237.

28- Khan W, Prithiviraj B, Smith DL. 2003. Photosynthetic response of corn and soybean to foliar application of salicylates. Journal Plant Physiolgy, 160: 485-492.

29- Khurama JPS and Cleland CF. 1992. Role of salicylic acid and benzoic acid in flowering of a photoperiod in sensitive strain, Lemna paucicostata LP6. Plant Physiology, 100: 1541-1546.

30- Korkmaz A, Uzunlu M, Demirkiran AR. 2007. Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress. Acta Physiol Plant, 29: 503-508.

31- Kovacik J, Grúz J, Backor M, Strnad M, Repcak M. 2009. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants. Plant Cell Reports, 28:135-143.

32- Kumar P, Dube D, Chauhan VS. 1999. Effect of salicylic acid on growth, development and some biochemical aspects of soybean (Glycine max L. Merrill). Indian Journal of Plant Physiology.

33- Leithy S, El-Meseiry T, Abdallah EF. 2006. Effect of biofertilizers, cell stabilizer and irrigation regime on Rosemary herbage oil yield and quality. Journal of Applied Research., 2: 773-779.

34- Martin-Mex R and Larque-Saavedra A. 2001. Effect of salicylic acid in clitoria (Clitoria ternatea L.) bioproductivity in Yucatan, Mexico. 28th Annual Meeting. Plant Growth Regulation Society of America. Miami Beach Florida, USA. July 1-5.

35- Martin-Mex R, Villanueva-Couoh T, Larque´-Saavedra A. 2005. Positive effect of salicylates on the flowering of African violet. Science Horticultural, 103: 499-502.

36- Nardi S, Pizzeghello D, Muscolo A, Vianello A. 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry. 34: 1527– 36.

37- Norman Q, Stephen L, Clive A, Rola A. 2003. Effects of humic acids derived from cattle, food and paper-waste vermicomposts on growth of greenhouse plants. Pedobiologia, 47: 741–744.

38- Padem H, Ocal A, Alan R. 1999. Effect of humic acid added foliar fertilizer on quality and nutrient content of eggplant and pepper seedlings. ISHS Acta Horticultural, 491.

39- Pietrosiuk A, Furmanowa M, Lata B. 2007. Catharanthus roseus: Micropropagation and in vitro techniques. Phytochem. Rev. 6: 459-473.

40- Rubio V, Bustos R, Irigoyen ML, Cardona-Lopez X, Rojas-Triana M, Paz-Ares J. 2009. Plant hormones and nutrient signaling. Plant Molecular Biolojy, 69(4): 361–73.

41- Sangeetha M, Singaram P, Uma Devi R. 2006. Effect of lignite humic acid and fertilizer on yield of onion and nutrient availability. International Union of Soil Science. 21, 163.

42- Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164(3): 317-322.

43- Shariff M. 2002. Effect of lignitic coal derived HA on growth and yield of wheat and maize in alkaline soil. Ph.D Thesis, NWFP Agriculture University Peshawar, Pakistan.

44- Singh B and Usha K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul, 141-137 ,39.

45- Sladky Z, Tichy V. 1959. Applications of humus substances to over ground organs of plants. Biolojy Plant, 1, 9-15.

46- Stevens J, Senaratna T, Sivasithamparam K. 2006. Salicylic acid induces salinity tolerance in tomato (Lycopersicon esculentum cv. Roma): associated changes in gas exchange, water relations and membrane stabilisation. Plant Growth Regulation, 49:77-83.

47- Tejada M and Gonzalez JL. 2003. Influence of foliar fertilization with amino acids and humic acids on productivity and quality of asparagus. Biological Agriculture and Horticulture, 21(3): 277-291.

48- Türkmen Ö, Dursun A, Turan M, Erdinç Ç. 2004. Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum L.) seedlings under saline soil conditionspp. Acta Agriculture Scandinavica, 7: 168-174.

49- Veronica M, Eva B, Angel-Maria Z, Elena A, Maria G, Marta F, Jose´-Maria GM. 2010. Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology, 167: 633-642.

50- Vital W, Teixeira R, Shigihara A and Dias FM. 2002. Organic manuring with pig biosolids withapplications of foliar biofertilizers in the cultivation of Thyme (Thymus vulgaris L.). Ecossistema., 27: 69-70.

51- Wendell KL, Wilson L, Jordan MA. 1993. Mitotic block in hela cells by vinblastine: ultrastructural changes in kinotochore-microtubule attachment and centrosomes. Journal Cell Science, 104: 261- 274.

52- Yuan S and Lin HH. 2008. Role of salicylic acid in plant abiotic stress. Z. Naturforsch. 63: 313-320.