بررسی ترکیبات مؤثره و اثرات ضد باکتریایی اسانس کرفس کوهی (Kelussia odoratissima Mozaff) و کلپوره (Teucrium polium) بر برخی از پاتوژن‌های مواد غذایی

نوع مقاله : مقالات پژوهشی

نویسندگان

• منصور مشرقی ¹
• مجید عزیزی ¹
• فاطمه عروجعلیان ²
• ناصر شاه طهماسبی ¹

¹ دانشگاه فردوسی مشهد

² دانشگاه تهران

https://doi.org/10.22067/jhorts4.v0i0.45086

چکیده

در این تحقیق اسانس کرفس کوهی (Kelussia odoratissima Mozaff) و کلپوره (Teucrium polium) به روش تقطیر با آب استخراج گردید. اسانس‌های بدست آمده با استفاده از دستگاه گاز کروماتوگراف (GC) و گاز کروماتوگراف متصل به طیف نگار جرمی (GC/MS)آنالیز شد و اجزای آن‌ها بر اساس شاخص بازداری و طیف جرمی تعیین گردید. سپس با استفاده از روش میکرودایلوشن (ریز رقت) و با استفاده از دستگاه خواننده الایزا خاصیت ضد باکتریایی اسانس‌های مورد نظر با تعیین حداقل غلظت بازدارندگی (MIC) و حداقل غلظت کشندگی (MBC) علیه برخی از باکتری‌های آلوده کننده مواد غذایی مانند Staphylococcus aureus، Bacillus cereus، Listeria monocytogenes، Escherichia coli O157H7 و Salmonella enterica و Pseudomonas aeruginosa بررسی شد. نتایج بدست آمده نشان داد که در کرفس کوهی اجزای مهم اسانس شامل (Z)-ligustilide، (Z)-3-butylidene-phthalide، limonene α-phellandrene و germacrene B بود. اجزای مهم اسانس کلپوره شامل β-caryophylene، Germacrene D، γ-cadinene، (Z)- nerolidol، camphor، β-pinene، α- camphene، linalool و α-humulene بود. MIC اسانس کرفس کوهی بین 31/0 میلی‌گرم بر میلی‌لیتر (مربوط به استافیلوکوکوس اورئوس) تا 5/2 میلی‌گرم بر میلی‌لیتر (سالمونلا انتریکا) متغیر بود در حالی‌که MIC اسانس کلپوره برابر 16/0 (مربوط به استافیلوکوکوس اورئوس) تا 25/1 میلی‌گرم بر میلی‌لیتر (مربوط به سالمونلا انتریکا) بود. با توجه به نتایج بدست آمده به نظر می‌رسد استفاده از گیاهان دارویی بومی در راستای کنترل موثر پاتوژن‌های مواد غذایی به عنوان یک روش مکمل که در عین حال اثر نامطلوب کم‌تری بر خواص ارگانولپتیکی داشته باشند سودمند باشد.

کلیدواژه‌ها

• کرفس کوهی
• کلپوره
• خواص ضد باکتریایی
• روش ریز رقت

20.1001.1.20084730.1393.28.4.6.3

عنوان مقاله [English]

Study on the Chemical Constituents and Antibacterial Activity of Kelussia odoratissima and Teucrium polium Essential Oils against Some Food Borne Pathogens

نویسندگان [English]

• Mansour Mashreghi ¹
• Majid Azizi ¹
• Fatemeh Oroojalian ²
• Naser Shahyahmasebi ¹

¹ Ferdowsi University of Mashhad

² University of Tehran

چکیده [English]

In this research the essential oils (EOs) of Kelussia odoratissima and Teucrium polium were extracted by hydrodistillation. Extracted essential oils constituents were analyzed by gas chromatograph (GC) and GC/mass spectrometry and the essential oils constituents identified according to retention time and mass spectrum. Then minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oils against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes، Escherichia coli O157H7, Salmonella enterica, and Pseudomonas aureogenosa were determined by microdilution technique using ELISA reader. The results showed that there are differences between the essential oils constituents as the main constituents in Kelussia odoratissima were (Z)-ligustilide, (Z)-3-butylidene-phthalide, limonene+-phellandren B. The main constituents of Teucrium polium essential oils were β-caryophylene, Germacrene D, γ-cadinene, (Z)- nerolidol, camphor, β-pinene, α- camphene, linalool, α-humulene. The MIC of Kelussia odoratissima EO was between 0.31 mg/ml (for S. aureus) to 2.5 mg/ml (for Salmonella enterica) but MIC of the Teucrium polium EO was between 0.16 mg/ml (for S. aureus) and 1.25 mg/ml (for Salmonella enterica). In conclusion, indigenous medicinal plants could be used for effective control of food borne pathogens as a complementary method that has less unfavorable effect on organoleptic attitudes of each products

کلیدواژه‌ها [English]

• Kelussia odoratissima
• Teucrium polium
• Antibacterial activity
• Microdilution method

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