Evaluation of Morphological Characteristics, Quantitative and Qualitative Yield of Echium amoenum as Affected by Plant Density and Application of Chemical (Urea) Fertilizer and Different Organic Fertilizers

Document Type : Research Article

Authors

• Mohammad Behzad Amiri ¹
• Parviz Rezvani Moghaddam ²
• Mohsen Jahan ³

¹ University of Gonabad

² Ferdowsi University of Mashhad, Iran

³ Ferdowsi University of Mashhad

https://doi.org/10.22067/jhorts4.v33i3.70323

Abstract

Introduction: Maintenance of soil fertility as a permanent bed for continuous production of agricultural products is one of the most important issues affecting the sustainability of food production. In order to achieve healthy food production, application of ecological inputs such as organic fertilizers are inevitable. Organic fertilizers are fertilizer compounds that contain one or more kinds of organic matter. They can improve the soil’s ability to hold water and nutrients. They create a beneficial environment for earthworms and microbial organisms that break the soil down into rich, fine humus. Compost is organic matter that has been decomposed and recycled as a fertilizer and soil amendment. Compost can greatly enhance the physical structure of soil. The addition of compost may provide greater drought resistance and more efficient water utilization. Vermicompost is the final product of composting organic material using different types of worms, such as red wigglers or earthworms, to create a homogenized blend of decomposed vegetable and food waste, bedding materials and manure. Vermicompost helps store nutrients and keeps them safe from leeching and irrigation, functioning to balance hormones within plant physiology, and adding beneficial biology to soil. Vermicompost improves soil aeration, enriches soil with micro-organisms (adding enzymes such as phosphatase and cellulose), attracts deep-burrowing earthworms already present in the soil and improves water holding capacity, enhances germination, plant growth and crop yield, improves root growth and structure. Cow manure is an excellent fertilizer containing nitrogen, phosphorus, potassium and other nutrients. It also adds organic matter to the soil which may improve soil structure, aeration, soil moisture-holding capacity, and water infiltration. Plant density is the number of individuals of a given plants that occurs within a given sample unit or study area. Planting density can impact the overall health of plants. Plantings that are too sparse (the density is too low) may be more susceptible to weeds, while planting that are too dense might force plants to compete over scarce nutrients and water and cause stunted growth. Despite of many researches on the effect of organic fertilizers and plant density on different crops, information on the effects of these factors for many medicinal plants is scarce, therefore, in this study effect of organic fertilizers and plant density on morphological characteristics and yield of Echium amoenum in a low input cropping system was studied.   
Materials and Methods: In order to evaluate the effect of different fertilizers on quantitative characteristics of Echium amoenum at different plant densities, a factorial experiment based on RCBD design with three replications was conducted in 2011-2013 growing seasons, in Ferdowsi University of Mashhad, Iran. Experimental factors included three plant densities (10, 5 and 3 plant per m2) and five different types of organic and chemical fertilizers (municipal waste compost, vermicompost, cow manure, chemical (urea) fertilizer and control).
Results and Discussion: The results showed that with reduction of plant density, effect of organic fertilizers on increasing the flower yield was exacerbated and at the lowest density, municipal waste compost, vermicompost and cow manures increased flower yield 112, 79 and 223% compared to control, respectively. In all studied densities, length of lateral branches in conditions of organic fertilizers application was more than control. Municipal waste compost, vermicompost, cow manure and chemical fertilizer increased total phenol 106, 54, 66 and 173% compared to control, respectively. In plant mean density (5 plant per m2), application of municipal waste compost, vermicompost and cow manure increased total anthocyanin 83, 74 and 57% compared to control, respectively. In general, result of this study showed that application of organic and chemical fertilizers at optimal plant densities, can improve growth characteristics and quantitative and qualitative yield of Echium amoenum.Organic fertilizers are the most significant resources for development of agricultural soil quality and increase in the yield of different medicinal plants. It has been reported that this ecological inputs provide favorable conditions for plant growth and development through improvement of physical, chemical and biological properties of the soil, therefore, it can be concluded that improvement of the most studied traits in the present study were due to use of organic fertilizers. Fallahi et al., (20) have reported the positive effects of organic fertilizers on the improvement of quantitative and qualitative characteristics in chamomile (Matricaria chamomilla L.). It seems that plants compete with each other over scarce nutrients and water in high plant density and cause stunted growth. Some other studies have reported that suitable plant density can increase the growth and yield of some medicinal plants such as coriander (Coriandrum sativum L.), Ajwain (Carum copticum L.), lemon balm (Melissa officinalis L.) and anise (Pimpinella anisum L.).
Conclusion: In general, results of this study showed that application of organic and chemical fertilizers at optimal plant densities, while improving growth and yield characteristics of Echium amoenum flower can be ensured health of production of this important medicinal plant.

Keywords

• Desirable Level of Plant
• Health of Production
• Compost
• Medicinal plant
• Ecofriendly Input

20.1001.1.20084730.1398.33.3.4.4

References

1- Ahmadabadi Z., Ghajar Sepanlou M., and Bahmanyar M.A. 2012. Effect of vermicomost application on amount of micro elements in soil and the content in the medicinal plant of Borago officinalis. Iranian Journal of Crops Improvement, 13: 1-12. (In Persian with English abstract)

2- Abu-Darwish M., Al-Ramamneh E.A.D., Salamon I., Abu-Dieyeh Z., Al-Nawaiseh M., and Albdour T. 2013. Determination of essential oil bioactive components and rosmarinic acid of Salvia officinalis cultivated under different intra-row spacing. Notulae Scientia Biologicae 5: 198-203.

3- Ahmadian A., Ghanbari A., and Galavi M. 2006. Effect of animal manure on quantitative and qualitative yield and chemical composition of essential oil in cumin (Cuminum cyminum). Iranian Journal of Field Crops Research 4: 1-10. (In Persian with English abstract)

4- Akbarinia A., Ghalavand A., Sefidkan F., Tahmasebi Z., Sharifi A., and Rezaee M. 2002. Evaluation of Copticum yield and oil in organic, conventional and integrated farming systems. 7nd agronomy and plant breeding of Iran, Seed and Plant Research Institute, Karaj. (In Persian with English abstract)

5- Akbar Nejad F., Astaraee A., Fotovat A., and Nassiri Mahallati M. 2010. Effect of municipal solid waste compost and sewage sludge on yield and yield component of Nigella sativa L. Iranian Journal of Field Crops Research 8: 767-771. (In Persian with English abstract)

6- Akhani A., Darzi M.T., and Haj Seyed Hadi M.R. 2012. Effects of biofertilizer and plant density on yield components and seed yield of coriander (Coriandrum sativum). International Journal of Agriculture and Crop Sciences 4: 1205-1211.

7- Al-Dein E., and Al-Ramamneh M. 2009. Plant growth strategies of Thymus vulgaris L. in response to population density. Industrial Crops and Products 30: 389-394.

8- Amiri M.B., Rezvani Moghaddam P., and Jahan M. 2016. Study the morphological characteristics affecting yield of Echium amoenum under different organic and chemical fertilizer and plant densities. Iranian Journal of Horticultural Science 47: 55-69. (In Persian with English abstract)

9- Anwar M., Patra D.D., Chand S., Alpesh K., Naqvi A., and Khanuja S.P.S. 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation and oil quality of French basil. Communication in Soil Science and Plant Analysis 36: 1737-1746.

10- Arancon N.Q., Edwards C.A., Bierman P., Welch C., and Metzger J.D. 2004. Influence of vermicompost on field strawberries. Bioresource Technology 93: 145-153.

11- Arancon N.Q., Galvis P.A., and Edwards A. 2005. Suppression of insect pest populations and damage to plants by vermicomposts. Bioresource Technology 96: 1137-1142.

12- Asadi Gh.A., Momen A., Noruzade Nameghi M., and Khoramdel S. 2014. Effect of different levels of organic and chemical fertilizers on yield and nitrogen efficiency of Plantago ovate Forsk. Iranian Journal of Agroecology 5: 373-382. (In Persian with English abstract)

13- Ashouri D., Nourhosieni Niaki S.A., Safarzadeh Vishkaei M.N., Aminpanah H., and Sharifi P. 2013. Effect of planting pattern and plant density on yield of Echium amoenum L. harvested in three years. Journal of Crop Production Research 261-276. (In Persian with English abstract)

14- Atiyeh R.M., Lee S.S., Edwards C.A., Arancon N.Q., and Metzger J. 2002. The influence of humic acid derived from earthworm-processed organic waste on plant growth. Bioresource Technology 84: 7-14.

15- Azizi M., Razavi M., Hasanzadeh Khayyat M.H., Lakzian A., and Neamati H. 2009. Effect of various levels of vermicompost and irrigation on morphologic characteristic of Matricaria recutita Var. Goral. Iranian Journal of Medicinal and Aromatic Plants 24: 82-93. (In Persian with English abstract)

16- Caliskan M.E., Kusman N., and Caliskan S. 2009. Effects of plant density on the yield and yield components of true potato seed (TPS) hybrids in early and main crop potato production systems. Field Crops Research 114: 223-232.

17- Chang Y.L. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal Agricultural Food Chemistry 50: 3713-3717.

18- Darzi M.T., Ghalavand A., Rejali F., and Sefidkon F. 2006. Effects of biofertilizers application on yield and yield components in fennel (Foeniculum vugare Mill.). Iranian Journal of Medicinal and Aromatic Plants 22: 278-292. (In Persian with English abstract)

19- Delate K. 2000. Heenah mahyah student from herb trail. Leopold center for sustainable agriculture. Annual Reports, Jowa State University. Ames, IA.

20- D'Hose T., Cougnon M., De Vliegher A., Vandecasteele B., Viaene N., Cornelis W., Van Bockstaele E., and Reheul D. 2014. The positive relationship between soil quality and crop production: A case study on the effect of farm compost application. Applied Soil Ecology 75: 189-198.

21- Doan T.T., Ngo P.T., Rumpel C., Nguyen B.V., and Jouquet P. 2013. Interactions between compost, vermicompost and earthworms influence plant growth and yield: A one-year greenhouse experiment. Scientia Horticulturae 160: 148-154.

22- Elgendy S.A., Hosni A.M., Omer E.A., and Reham M.S. 2001. Variation in herbage yield, essential oil yield and oil composition on sweet basil (Ocimum bacilicum) grown organically in newly reclaimed and in Egypt. Arab Universities Journal of Agricultural Science 9: 915-933.

23- Fallahi J., Koocheki A., and Rezvani Moghaddam P. 2008. Investigating the effects of organic fertilizers on quantity index and the amount of essential oil and chamazulene in chamomile (Matricaria recutita). Iranian Journal of Water, Soil and Plant in Agriculture 8: 157-168. (In Persian with English abstract)

24- Faraji M., and Poursakhi K. 2018. Effects of biological and organic fertilizers on morphological and phytochemical characteristics of Iranian borage (Echium amoenum Fisch. & C.A.Mey.). Iranian Journal of Medicinal and Aromatic Plants 34: 50-61. (In Persian with English abstract)

25- Farhang Meher S., Akbari Sh., and Rezvan Beidokhti Sh. 2014. Effect of planting date and plant density on flower yield and some morphological characteristics of Matricaria chamomilla L. Journal of Plant Ecophysiology 6: 79-87. (In Persian with English abstract)

26- Ghilavizadeh A., Darzi M.T., Haj Seyed Hadi M.R., and Rejali F. 2012. Influence of plant growth promoter bacteria and plant density on yield components and seed yield of ajowan (Carum copticum). International Journal of Agriculture and Crop Sciences 4: 1255-1260.

27- Gutierrez F.A., Santiago J., Molina J.A.M., Nafate C.C., Abud M., Llaven M.A.O., Rincon R., and Dendooven L. 2007. Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum). Bioresource Technology 98: 2781-2786.

28- Haj Seyyed Hadi M.R., Darzi M.T., Riazi Gh.H. and Ghandarhari Z. 2013. Evaluation of effect of vermicompost and aminoacids on yield and yield components of Matricaria chemmomilla. Iranian Journal of Plant and Ecosystem 33: 67-80. (In Persian with English abstract)

29- Hasanvand H., Siadat S.A., Bakhshandeh A.M., Moradi Telavat M.R., and Poshtdar A. 2018. Evaluation of yield and some physiological characteristics of borage (Borago officinalis L.) under plant density and sowig dates in Ahwaz region. Iranian Journal of Medicinal and Aromatic Plants 34: 1-16. (In Persian with English abstract)

30- Heidari M.R., Azad E.M., and Mehrabani M. 2006. Evaluation of the analgesic effect of Echium amoenum Fisch & C.A. Mey. Extract in mice: Possible mechanism involved. Journal of Ethnopharmacology 103: 345-349.

31- Heidari F., Zehtab-Salmasi S., Javanshir A., Aliari H., and Dadpour M.R. 2008. The effect of plant density on yield and production of essential oil of peppermint (Mentha piperita L.). Journal of Science and Technology of Agriculture and Natural Resources 12: 501-510. (In Persian with English abstract)

32- Hekmati M., Hadian J., and Tabaei Aghdaei S.R. 2012. Evaluating the effect of planting density on yield and morphology of savory (Satureja khuzistanica Jamzad). Annals of Biological Research 3: 4017-4022.

33- Hosseinpour M., Pirzad A., Habibi H., and Fotokian M.H. 2011. Effect of biological nitrogen fertilizer (Azotobacter) and plant density on yield, yield components and essential oil of anise. Iranian Journal of Agricultural Science and Sustainable Production 2: 69-86. (In Persian with English abstract)

34- Ibrahim H.M. 2012. Response of some sunflower hybrids to different levels of plant density. APCBEE Procedia 4: 175-182.

35- Karami A., Sepehri A., Hemzei J., and Salimi Gh. 2011. Effect of nitrogen and phosphorous biofertilizers on quantitative and qualitative traits of borage (Borago officinalis L.) under water deficit stress. Plant Production Technology 11: 37-50. (In Persian with English abstract)

36- Khalesro Sh., Ghalavand A., Sefidkon F., and Asgharzadeh A. 2012. The effect of biological and organic inputs on quantity and quality of essential oil and some elements content of anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants 27: 551-560. (In Persian with English abstract)

37- Khandan A., Astaraee A., Nassiri Mahallati M., and Fotovvat A. 2004. Effects of organic and inorganic fertilizers on yield and yield components of Plantago ovata Forsk. Iranian Journal of Field Crops Research 3: 245-253. (In Persian with English abstract)

38- Koocheki A., Amirmoradi Sh., Shabahang J., and Kalantari Khandani S. 2013. Effect of organic fertilizers on quantitative and qualitative characteristics of Plantago ovata Forssk., Alyssum homolocarpum L., Lepidium perfoilatum L., and Lalementia iberica L. Agroecology 5: 16-26. (In Persian with English abstract)

39- Lakhdar A., Falleh H., Ouni Y., Oueslati S., Debez A., Ksouri R., and Abdelly Ch. 2011. Municipal solid waste compost application improves productivity, polyphenol content, and antioxidant activity of Mesembryanthemum edule. Journal of Hazardous Materials 191: 373-379.

40- Liuc J., and Pank B. 2005. Effect of vermicompost and fertility levels on growth and oil yield of Roman chamomile. Scientia Pharmaceutica 46: 63-69.

41- Mafakheri S., Omidbaigi R., Sefidkon F., and Rejali F. 2012. Effect of vermicompost, biophosphate and azotobacter on quantity and quality of essential oil of Dracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants 27: 596-605. (In Persian with English abstract)

42- Mehrabani M., Ghassemi N., Sajjadi S.E., Ghannadi A., and Shams-Ardakani M. 2005. Main phenolic compounds of petals of Echium amoenum fisch. And C.A. Mey., a famous medicinal plant of Iran. Daru 13: 65-69.

43- Mir B., Ghanbari A., Ravan S., and Asgharipour M. 2011. Effects of plant density and sowing date on yield and yield components of Hibiscus sabdarij in Zabol region. Advances in Environmental Biology 5: 1156-1161.

44- Miri H., and Darzi M.T. 2018. Effects of manure and phosphate solubilizing biofertilizer on growth, yield and essential oil quality of dragonhead (Dracocephalum moldavica L.) in Firouzkuh region. Iranian Journal of Field Crop Science 49: 35-45. (In Persian with English abstract)

45- Moradi R., Nassiri Mahallati M., Rezvani Moghaddam P., Lakzian A., and Nejadali A. 2011. Effect of biological and organic fertilizers on quantitative and qualitative o oil in Foeniculum vulgare Mill. Journal of Horticultural Science 25: 25-33. (In Persian with English abstract)

46- Motta S.R., and Maggiore T. 2013. Evaluation of nitrogen management in maize cultivation grown on soil amended with sewage sludge and urea. European Journal of Agronomy 45: 59-67.

47- Muanda F.N., Soulimani R., Diop B., and Dicko A. 2011. Study on chemical composition and biological activities of essential oil and extracts from stevia rebaudiana Bertoni leaves. Journal of Food Science and Technology 44: 1865-1872.

48- Nabizadeh E., Habibi H., and Hosainpour M. 2012. The effect of fertilizers and biological nitrogen and planting density on yield quality and quantity Pimpinella anisum L. European Journal of Experimental Biology 2: 1326-1336.

49- Najafpoor Navaee M. 2002. Evaluation of effect of phosphorous and nitrogen fertilizers on seed yield of Echium amoenum. Iranian Journal of Medical and Aromatic Plants 13: 41-50. (In Persian with English abstract)

50- Ndabamenye T., Van Asten P.J.A., Blomme G., Vanlauwe B., Swennen R., Annandale J.G., and Barnard R.O. 2013. Ecological characteristics and cultivar influence optimal plant density of East African highland bananas (Musa spp., AAA-EA) in low input cropping systems. Scientia Horticulturae 150: 299-311.

51- Nemati Darbandi H., Azizi M., Mohammadi S., and Karimpoor S. 2014. Evaluation of effect of foliar spray with different consentrations of vermicompost on morphological traits, essential oil percentage and yield of Melissa officinalis L. Iranian Journal of Horticulture Science 27: 411-417. (In Persian with English abstract)

52- Niknejad M., Lebaschy M.H., Jaimand K., and Hatami F. 2013. Effect of organic and chemical fertilizers on essential oil of Matricaria chamomilla L. Iranian Journal of Medicinal and Aromatic Plants 29: 373-386. (In Persian with English abstract)

53- Nooriyan Soroor E., Rouzbehan Y., and Alipour D. 2013. Effect of Echium amoenum extract on the growth rate and fermentation parameters of Mehraban lambs. Animal Feed Science and Technology 184: 49-57.

54- Ordone A.A.L. 2008. Antioxidant activities of sechium edule swartz extracts. Journal Agricultural Food Chemistry 97: 452-458.

55- Padmavathiamma P.K., Li L.Y., and Kumari U.R. 2008. An experimental study of vermin-biowaste composting for agriculture soil improvement. Bioresource Technology 99: 1672-1681.

56- Raja Sekar K., and Karmegan N. 2010. Earthworm casts as an alternate carrier material for biofertilizers: Assessment of endurance and viability of Azotobacter chroococcum, Bacillus megaterium and Rhizobium leguminosarum. Scientia Horticulturae 124: 286-289.

57- Rantala P.R., Vaajasaari K., Juvonen R., Schultz E., Joutti A., and Makela-kurtto R. 1999. Composting of forest industry wastewater sludges for agricultural use. Water Science Technology 40: 187-194.

58- Ren X., Zhang L., Du M., Evers J.B., Van der Werf W., Tian X., and Li Zh. 2013. Managing mepiquat chloride and plant density for optimal yield and quality of cotton. Field Crops Research 149: 1-10.

59- Rezaee M., and Baradaran R. 2011. Effects of biofertilizers on the yield and yield components of pot marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants 29: 635-650. (In Persian with English abstract)

60- Rezakhani A., and Haj Seyed Hadi M.R. 2017. Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum L.). Iranian Journal of Field Crop Science 48: 777-786. (In Persian with English abstract)

61- Rezvani Moghaddam P., Aminghafuri A., Bakhshaee S., and Jafari L. 2013. Evaluation of effect of biofetilizer and organic fertilizer on some quantitative characteristics and amount of oil of Satureja hortensis L. Agroecology 5: 105-112. (In Persian with English abstract)

62- Sabahi H., Takafooyan J., Mahdavi Damghani A.M., and Liaghati H. 2010. Effects of integrated application of farmyard manure, plant growth promoting rhizobacteria and chemical fertilizers on production of canola (Brassica napus L.) in saline soil of Qum. Agroecology 2: 287-291. (In Persian with English abstract)

63- Saglam C., Atakisi I., Turhan H., Kaba S., Arslanoglu F., and Onemli F. 2004. Effect of propagation method, plant density, and age on lemon balm (Melissa officinalis) herb and oil yield. New Zealand Journal of Crop and Horticultural Science 32: 419-423.

64- Salehi A., Ghalavand A., Sefidkon F., and Asgharzade A. 2011. The effect of zeolite, PGPR and vermicompost application on N, P, K concentration, essential oil content and yield in organic cultivation of german chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants 27: 200-214. (In Persian with English abstract

65- Sangwan P., Kaushik C.P., and Garg V.K. 2008. Feasibility of utilization of horse dung spiked filter cake in vermicomposters using exotic earthworm Eisenia foetida. Bioresource Technology 99: 2442-2448.

66- Sayyah M., Boostani H., Pakseresht S., and Malaieri A. 2009. Efficacy of aqueous extract of Echium amoenum in treatment of obsessive-compulsive disorder. Progress in Neuro-Psychopharmacology and Biological Psychiatry 33: 1513-1516.

67- Schiffer M.C., Ronzelli Junior P., and Koehler H.S. 1993. Influence of organic fertilizeration on the biomass, yield composition of the essential oil of Achillea millefolium L. Acta Horticulture 331: 109-114.

68- Shamsodin S.M., Maghsudi K., Farahbakhsh H., and Naseralavi M. 2007. Compost and control of soil erosion. 2nd National Congress of Ecological Agriculture, 25-26 October, Iran, Gorgan. (In Persian with English abstract)

69- Sharpley A.N., McDowell R., and Kleinman P.J.A. 2004. Amounts, forms, and solubility of phosphorus in soils receiving manure. Soil Science Society of American Journal 68: 2048-2057.

70- Shehata S.A., Gharib A.A., Mohamed M., El-Mogy Abdel Gawad K.F., and Emad A.S. 2011. Influence of compost, amino and humic acid on the growth, yield and chemical parameters of strawberries. Journal of Medicinal Plants Research 5: 2304-2308.

71- Sheikhpour S., Sirousmehr A., and Fakheri B. 2017. Effect of nitrogen and nitroxin levels on quantitative traits of borage (Borago officinalis L.) under Zabol conditions. Journal of Agricultural Crops Production 19: 933-945. (In Persian with English abstract)

72- Shimada K., Fujikawa K., Yahara K., and Nakamura T. 1992. Antioxidative properties of xanthin on autoxidation of soybean oil in cyclodetrin emulsion. Journal of Agricultural and Food Chemistry 40: 945-948.

73- Tabrizi L. 2004. The effect of water stress and manure on yield, yield components and quality characteristics of Plantago psyllium. MS Thesis. Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. (In Persian with English abstract)

74- Weber J., Kocowicz A., Bekier J., Jamroz E., Tyszka R., Debicka M., Parylak D., and Kordas L. 2014. The effect of a sandy soil amendment with municipal solid waste (MSW) compost on nitrogen uptake efficiency by plants. European Journal of Agronomy 54: 54-60.