Study of Growth Traits of Scindapsus spp. as Influenced By Fertigation of Humic Acid and Folvic Acid

Document Type : Research Article

Authors

• Rasul AbaszadehFaruji
• Mahmood Shoor
• Ali Tehranifar
• Bahram Abedy

Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.22067/jhs.2021.61456.0

Abstract

Introduction
 Unbalanced and frequent use of chemical fertilizers and pesticides results in the degradation of soil physicochemical properties, loss of soil-born organisms, reduction of quality of produced crops and reduction of yield plant. Nowadays, due to environmental considerations of chemical fertilizers, use of organic acids for quantitative and qualitative improvement of crops has been increased. Humic materials are natural organic compounds that contain 50 to 90% of organic matters of peat, wood coal and rotten material, as well as non-living organic matters of aquatic and terrestrial ecosystems. Humic compounds indirectly increase soil fertility by providing micro- and macro-elements for root, improving soil structure, increasing medium permeability to water and air, increasing soil microbial population and beneficial microorganisms, increasing cation exchange capacity and the ability to buffer pH of medium or nutrient solution, and providing some special substances for plant roots such as nucleic acids and acetamides.
Materials and Methods
 In order to investigate the influence of humic and fulvic acids on some growth characteristics in ornamental plant of Scindapsus spp., an experiment was conducted based on a completely randomized design with three replications at greenhouse in Ferdowsi University of Mashhad during 2014-2015. The first factor was humic acid in four levels of 0, 0.2, 0.5, 1 g/l, and the second factor was fulvic acid in four levels of 0, 0.2, 0.5, 1 g/l. Cuttings were taken from the plant in late March. Two weeks after transferring the rooted cuttings to pot, the treatments were applied on plants via fertigation. The traits measured in the experiment included plant height, mean number of nodes, internode length, leaf number, leaf length, leaf width, leaf area, root length, root fresh weight, leaf fresh weight, shoot fresh weight, aerial organ fresh weight, root volume, aerial organ volume, root dry weight, leaf dry weight, shoot dry weight, aerial organ dry weight, fresh and dry weights ratios of aerial organ to fresh weight. Statistical analysis of data was performed by 8-JMP software. LSD test was used to compare the means of the data.
Results and Discussion
 Based on the results obtained, the combined use of humic acid and fulvic acid had a significant effect on height, fresh and dry weights of leaf, shoot, aerial organ and root, number of node and leaf and volume of aerial organ, fresh and dry weights of root, length and volume of root and the ratio of fresh and dry weights of aerial organ to root. Thus, combined use of humic acid and fulvic acid caused an increase in all the measured traits compared to the control treatment. Furthermore, an increase was observed in the length, width, and area of leaf, and internode length as the result of application of humic substances when compared to the control treatment. Organic fertilizers increase plant growth by improving soil conditions and increasing availability of plant to nutrients. Humic compounds cause changes in the specific distribution of cytokinins, polyamines and ATP by affecting the activity of root H⁺-ATPase and the distribution of root nitrate in the stem, thus affecting the growth of the plant stem. Humic materials increase plant growth by using different mechanisms such as hormonal effects, direct effect on plant cell metabolism, increase of cell division, chelating power and nutrient uptake, increase of soil ventilation and enhancement of plant photosynthesis by increasing Rubisco enzyme activity. Increasing nitrogen uptake leads to the increase in the growth of shoots and aerial organs. It also increases the number of branches in plants and increases the production of dry matter by increasing the production of photosynthetic materials.
Conclusion
 In general, according to the results of this experiment, it can be concluded that application of humic substances (humic acid and fulvic acid) together, improved the growth characteristics of the plant and led to the better growth of the plant; therefore, these materials can be a good alternative to plant growth-stimulating chemical fertilizers.

Keywords

• Houseplants
• Morphological traits
• Organic materials
• Soil fertility

20.1001.1.20084730.1402.37.1.2.6

Main Subjects

• Ornamental plants

References

1. AbaszadehFaruji, , Shoor, M., Tehranifar, A., Abedi, B., & Safari, N. (2018). Effects of humic acid and fulvic acid on some morphological characteristics of geranium. Journal of Horticultural Science 32(1): 35-50. (In Persian with English abstract). https://doi.org/10.22067/JHORTS4.V31I3.57849.
2. Adani, F., Genevini, P., Zaccheo, P., & Zocchi, G. (1998). The effect of humic acid on tomato plant growth and mineral nutrition. Journal Plant Nutrition 21: 561-575. https://doi.org/1080/01904169809365424.
3. Adediran, J.A., Taiwa, L.B., Akande, M.O., Sobulo, R.A., & Idown, O.J. (2004). Application of organic and inorganic fertilizer for sustainable maize and coupea yield in Nigeria. Journal of Plant Nutrition 27: 1163-1181. https://doi.org/10.1081/PLN-120038542.
4. Aiken, G.R., Mcknight, D.M., Wershaw, R.L., & Mccarthy, P. (1985). Humic substances in soil, sediment and water. Wiley-Interscience. New York. U.S.A. https://doi.org/1002/gj.3350210213.
5. Albayrak, S., & Camas, N. (2005). Effect of different levels and application times of humic acid on root and leafyield and yield component of Forage turnip (Brassica rapa). Journal of Agronomy 42: 130-133. https://doi.org/10.3923/ja.2005.130.133.
6. Arteca, R.N. (1996). Plant growth substances: Principles and applications. Chapman and Hall. New York. pp. 250-
7. Astaraei, A.R., & Ivani, R. (2008). Effect of organic sources as foliar spray and root media on nutrition if cowpea plant. Am.-Euras. Agriculture Environment Science 3(3): 352-356.
8. Atiyeh, R.M., Arancon, N., Edwards, C.A., & Metzger, J.D. (2002). The influence of humic acids derived from earthworm processed organic wastes on plant growth. Bioresource Technology 84(1): 7-14. https://doi.org/10.1016/S0960-8524(02)00017-2.
9. Azarpou, A. (2012). Evaluation and determination of the best time of priming and priming solution levels for germination indexes of fenugreek (Trigonella foenumgracum ). Journal of Agricultural and Biological Science 7(3): 141-146.
10. Azizi, M., & Safa, Z. (2016). Effect of humic acid and nanocardopharmes spraying on morphological traits, yield and essential oil of Nigella sativa Journal of Horticulture Science 30(3): 671-680. https://doi.org/10.22067/JHORTS4.V0I0.41136.
11. Bahalu, Z., Rizi, S., Rabi, Gh., & Saedi, K. (2018). Effect of vermicompost and humic acid on qualitative and quantitative traits of Eustoma grandiflorum after transfer of transmission. Journal of Science and Technology of Greenhouse Culture -Isfahan University of Technology 8(4): 17-24.
12. Balakumbahan, R., & Rajamani, K. (2010). Effectof bio stimulants on growth and yield of Senna (Cassia angustifolia L.). Journal of Horticultural Sciences 2(1): 16-18.
13. Barghmadi, K., & Najafi, Sh. (2015). Effect of different levels of nitroxin and humic acid on some quantitative characteristics and essential oil of Carum copticum (L.). C .B . Clarke. Journal of Horticultural Science 29(3): 332-341. https://doi.org/22067/jhorts4.v0i0.22523.
14. Befrozfar, M.R., Habibi, D., Asgharzadeh, A., Sadeghi-Shoae, M., & Tookalloo, M.R. (2013). Vermicompost, plant growth promoting bacteria and humic acid can affect the growth and essence of sweet basil (Ocimum basilicum ). Annals of Biological Research 4(2): 8-12. https://doi.org/10.13140/RG.2.1.1897.4882.
15. Bellapart, C. (1996). New biological agriculture in equilibrium with chemical agriculture. Barcelona, Spain: Editions Mundi–Press.
16. Bollo, E. (1999). Earthworm culture, a recycling alternative. Barcelona, Spain: Editions Mundi–Press.
17. Bronick, E.J., & Lai, R. (2005). Soil structure and management: A review. Geoderma 124: 3-22. https://doi.org/10.1016/j.geoderma.2004.03.005.
18. Calvo, P., Nelson, L., & Kloepper, J.W. (2014). Agricultural uses of plant biostimulants. Plant and Soil 3-41. https://doi.org/10.1007/s11104-014-2131-8.
19. Cavender, N.D., Atiyeh, R.M., & Knee, M. (2003). Vermicompost stimulates mycorrhizal colonization of roots of Sorghum bicolor at the expense of plant growth. Pedobiologia 47: 85-89. https://doi.org/10.1078/0031-4056-00172.
20. Chen, Y., Clapp, C.E., & Magen, H. (2004). Mechanisms of plant growth stimulation by humic substances: The role of organo-iron complexes. Soil Science and Plant Nutrition 50: 1089-109. https://doi.org/10.1080/00380768.2004.10408579.
21. Chen, Y., & Aviad, T. (1990). Effects of humic substances on plant growth. PP. 161-186. In: MacCarthy et al. (Eds.), Humic Substances in Soil and Crop Science: Selected Readings. SSSA and ASA. Madison. WI. USA. https://doi.org/2136/1990.humicsubstances.c7.
22. Clapp, CE., Hayes, MHB., & Swift, RS. (1993). Isolation, fractionation, functionalities, and concepts of structure of soil organic macromolecules, in A J. Beck, K.C. Jones, M.B.H. Hayes, and U. Mingelgrin (eds.), Organic substances in soil and water. Royal Society of Chemistry, Cambridge.
23. Cooper, R., Chunhua liu, J., & Fisher, D.S. (1998). Influence of humic substances on rooting and nutrient content of creeping bentgrass. Crop Science 38(6): 1639. https://doi.org/2135/cropsci1998.0011183X003800060037x.
24. Dastyaran, M., & Farahi, M.H. (2014). The effect of humic acid and putrescine on vegetative properties and flower life of roses in soil culture system. Journal of Science and Technology of Greenhouse Culture. Soilless Culture Research Center 5(20): 243-252.
25. David, P.P., Nelson, P.V., & Sanders, C.D. (1994). A humic acid improves growth of tomato seedling in solution culture. Journal of Plant Nutrition 17: 173-184. https://doi.org/10.1080/01904169409364717.
26. Delfine, S., Tognetti, R., Desiderio, E., & Alvino, A. (2005). Effect of foliar application of N and humic acids ongrowth and yield of durum wheat. Agronomy for Sustainable Development 25: 183-191. https://doi.org/1051/agro:2005017.
27. Dursun, A., Guvenc, I., & Turan, M. (2002). Effects of different levels of humic acid on seedling growth and macro and micronutrient contents of tomato and eggplant. Acta Agrobotanica 56: 81-88. https://doi.org/5586/aa.2002.046.
28. El-Ghamry, , Kamar, M., El- Hai, A., & Khalid, G. (2009). Amino and humic acids promote growth, yield and disease resistance of Broad bean (Faba vulgaris L.) cultivated in clayey soil. Australian Journal of Basic and Applied Sciences 3(2): 731-739.
29. El-Kenawy, A. (2017). Effect of chitosan, salicylic acid and fulvic acid on vegetative growth, yield and fruit quality of Thompson seedless grapevines. Egyption Journal Horticulture 44(1): 45-59. https://doi.org/10.21608/EJOH.2017.1104.1007.
30. Esringu, A., Sezen, I., Aytatli, B., & Ercisli, S. (2015). Effect of humic and fulvic acid application on growth parameters in Impatiens walleriana Akademik Ziraat 4(1): 37-42.
31. Fazel Tehrani, , Ilkai, M.N., & Mostafavi, Kh. (2017). Study of the effect of humic acid and tea compost on leaf basil characteristics. Journal of Agronomy and Plant Breeding 13(4): 65-73.
32. Ghasemi Ghahsareh, , & Kafi, M. (2010). Scientific and Practical Floriculture. V.1. 310 p
33. Ghorbani, S., Khazayi, H.M, Kafi, M., & Banayanaval, M. (2010). Effect of humic acid in irrigation water on yield and yield components of maize (Zea mays). Journal of Ecology 2(1):131-123. (In Persian with English abstract). https://doi.org/10.22067/jag.v2i1.7608.
34. Golchin, A., Besharati, H., & Shafiyi, S. (2010). Factors affecting the dynamics of organic materials and its effect on soil properties. The first congress of fertilizer challenges in Iran.
35. Guerrero, A. (1996). Soil fertilizers and crop fertilization. Bilbao, Spain: Editions Mundi–Press.
36. Harper, S.M., Kerven, G.L., Edwards, D.G., & Ostatek Boczyski, Z. (2000). Characterizyion on fulvic and humic acid from leavesof eucalyptus comaldulensis and from decomposed hey. Soil Biochemistry 32: 1331-1336. https://doi.org/10.1016/S0038-0717(00)00021-3.
37. Honorato, R. (1993). Edaphology manual. Santiago, Chile: Editorial Universitaria S.A.
38. Jack, AH., & Evans, MR. (2000). Humic acid seed and substrate treatments promote seedling root development. Horticultural Science 35(7): 1231-1233. https://doi.org/21273/HORTSCI.35.7.1231.
39. Kamali, M., Shoor, M., & Salahvarzi, Y. (2012). Studying the effect of salt stress on physio-morphological characteristics of C₄ plants of tuberculate (Gomphrena globosa L.) and Amaranthus tricolor (Amaranthus tricolor. L.) under different levels of carbon dioxide. Ferdowsi University of Mashhad. M.Sc Thesis.
40. Kamari Shahmaleki, S., Peyvast, Gh., & Ghasemnazhad, M. (2012). Effect of humic acid on growth and yield of tomato cv. Isabela. Horticultural Sciences (Agriculture Science and Technology) 26(4): 358-363. https://doi.org/22067/JHORTS4.V0I0.18148.
41. Khazaie, H.R., EyshiRezaie, E., & Bannayan, M. (2011). Application times and concentration of humic acid impact on above ground biomass and oil production of hyssop (Hyssopus officinalis). Medicinal Plants Research 5(20): 5148-5154.
42. Khoram Del, S., Koochaki, A., Nassiri Mahalati, M., & Ghorbani, R. (2007). Application effects of biofertilizers on the growth indices of black cumin (Nigella sativa). Journal of Iranian Field Crop Research 2(6): 285-294. https://doi.org/10.22067/GSC.V6I2.2435.
43. Khoramdel, S., Kuchaki, A., Nasiri Mahalati, M., & Ghorbani, R. (2008). Effect of biofertilizers on growth indices of black cumin (Nigella sativa). Journal of Agriculture Research 6: 294-285. (In Persian with English abstract). https://doi.org/10.22067/gsc.v6i2.2435.
44. Kochaki, A. (1996). From the Green Revolution to the Cost of the Revolution. Conflict or Understanding. The 4^th Iranian Congress of Agronomy and Plant Breeding. In Persian.
45. Koram Ghe-Farokhi, A., Rahimi, A., Torabi, B., & Madah Hoseini, Sh. (2015). The effect of application of humic acid, spraying leaves of compost tea and vermicelli on the adsorption of chlorophyll elements and content of Cartamus tinctorius Journal of Oil Plants Production 2(1): 71-84.
46. Malan, CH. No year. Review: Humic and Fulvic Acids. A Practical Approach.
47. Marschner, H. (2002). Mineral nutrition of higher plants under saline soil conditions. Acta Agriculturae Scandinavica, Section B— Soil & Plant Science 54: 168-174.
48. Michael Karr, M. (2001). Oxidized lignites and extracts from oxidized lignites in agriculture. Available online at: http://humates.com/Humates.Agriculture.Karr.pdf.
49. Moradi, M., Pasari, B., & Fayyaz, F. (2017). The effects of fulvic acid application on seed and oil yield of safflower cultivars. Journal of Central European Agriculture 18(3): 584-597. https://doi.org/5513/JCEA01/18.3.1933.
50. Nadi, S., Pizzegheloo Musolo, A., & Vianello, A. (2002). Physiological effect of humic substances on higher plants. Soil Biology Biochemical 34: 1527-1536. https://doi.org/1016/S0038-0717(02)00174-8.
51. Nardi, S., Pizzeghello, D., Muscolo, A., & Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry 34: 1527–1536. https://doi.org/10.1016/S0038-0717(02)00174-8.
52. Nikbakht, A., Etemadi, N., & Yazdani, B. (2010). Application of humic and flvic acids in nutrient solution affects postharvest characteristics of Gerbera jamesonnii Acta Horticulturae 934(934): 495-499. https://doi.org/10.17660/ActaHortic.2012.934.65.
53. Piraste Anoshe, , Emam, Y., & Jamali Ramin, F. (2010). Comparison of the effect of bio-fertilizers with chemical fertilizers on growth, yield of sunflower oil in different levels of drought stress. Agroecology Journal 2(3): 492-501. https://doi.org/10.22067/JAG.V2I3.7663.
54. Reynolds, A.G., Wardle, D.A., Drought, B., & Cantwell, R. (1995). Gro-mate soil amendment improves growth of greenhouse-grown 'Chardonnay' grapevines. Horticulture Science 30: 539-554.
55. Roohani, N.S., Nemati, S.H., Moghadam, M., & Ardakanian, V. (2014). The role of humic acid on morphological, physiological and biochemical characteristics of three varieties of radish (Raphanus sativus L.) under salt stress. Ferdowsi University of Mashhad. M.Sc Thesis.
56. Rubio, V., Bustos, R., Irigoyen, M.L., Cardona-Lopez, X., Rojas-Triana, M., & Paz-Ares, J. (2009). Plant hormones and nutrient signaling. Plant Molecular Biolojy 69(4): 361–73. https://doi.org/10.1007/s11103-008-9380-y.
57. Sabzevari, S., Khazai, H.R., & Kafi, M. (2009). The effect of humic acid on root and shoot growth of Sayonez and Sabalan cultivars of wheat (Triticum aestivum ). Journal of Soil and Water (Agricultural Science and Technology) 23(2): 87-94. https://doi.org/10.22067/jsw.v0i0.1731.
58. Saleh Rastin, N. (1999). Biological fertilizers. Journal of Soil and Water Special Publication Research Institute. Agricultural Extension and Education 12(3): 17-26.
59. Samavat, S., & Malakoti, M. (2006). Necessitates the use of organic acids (humic and Fulvic) to increase the quantity and quality of agricultural products. Technical Bulletin No. 463. Senate Publications. Tehran. Iran. (In Persian with English abstract)
60. Samavat, S., & Malakouti, M.J. (2005). Necessity of production and utilization of organic acids to increase quality and quantity of agricultural products. Sana Publisher, Tehran. (In Persian with English abstract)
61. Shahbazi, M., Chamani, A., Shahbazi, M., Mostafavi, M., & Poorbirami, H.I.Y. (2012). Investigating the effect of different substrates of vermicompost, peat, and coconut cultivation on the carnation growth and flowering characteristics. Journal of Agricultural Knowledge and Sustainable Production 22(3): 127-136.
62. Sharif, M., Khattak, R.A., & Sarir, M.S. (2002). Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Communications in Soil Science and Plant Analysis 33: 3567-3580. https://doi.org/1081/CSS-120015906.
63. Sidari, M., Ronzello, G., Vecchio, G., & Muscolo, A. (2008). Influence of slope aspects on soil chemical and biochemical properties in a Pinus laricio forest ecosystem of Aspromonte (Southern Italy). European Journal of Soil Biology 44(4): 364-372. https://doi.org/1016/j.ejsobi.2008.05.001.
64. Soleimani Aghdam, M., Hassanpour-Aghdam, M., Paliyat, G., & Farmani, B. (2012). The language of calcium in postharvest life of fruits, vegetables and flowers. Science Horticulture 144: 102-115. https://doi.org/1016/j.scienta.2012.07.007.
65. Stevenson, F.J. (1994). Organic forms of soil nitrogen. In: wiley john, editor. Humic chemistry: genesis, composition, reaction. New york: 59–95.
66. Taghizadeh, M., Ahsani Iravani, V., & Asadi, L. (2013). Investigating the effects of vermicompost application on improving quality and quantity of horticultural products. 1^st Symposium on New Discussion in Horticultural Science. Scientific Association of Horticultural Engineering. Jahrom University.
67. Tattini, M., Bertoni, P., Landi, A., & Traversi, M.L. (1991). Effect of humic acids on growth and biomass partitioning of container-grown olive plants. Acta Horticulture 294: 75-80. https://doi.org/10.17660/ActaHortic.1991.294.7.
68. Terzi, A., Coban, S., Yildiz, F., Ates, M., Bitiren, M., Taskin, A., & Aksoy, N. (2010). Protective effects of black cumin (Nigella sativa) on intestinal ischemia-reperfusion injury in rats. Journal of Investigative Surgery 23(1): 21. https://doi.org/10.3109/08941930903469375.
69. Turkmen, O., Dursun, A., Turan, M., & Erdinc, C. (2004). Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum) seedlings. Acta Agriculturae Scandinavica. Section B—Soil and Plant Science 54: 168-174. https://doi.org/10.1080/09064710310022014.
70. Valdrighi, M.M., Pear, A., Agnolucci, M., Frassinetti, S., Lunardi, D., & Vallini, G. (1996). Effects of compost-derived humic acids on vegetable biomass production and microbial growth within a plant (Cichorium intybus)-soil system: A comparative study. Agriculture Ecosystem Environmental 58: 133-144. https://doi.org/10.1016/0167-8809(96)01031-6.
71. Vermeer, A.W.P. (1996). Interactions between humic acid and hematite and their effects on metal ion speciation. The Netherlands: Wageningen University. (phd thesis).
72. Veronica, M., Eva, B., Angel-Maria, Z., Elena, A., Maria, G., Marta, F., & Jose´-Maria, GM. (2010). Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology 167: 633-642. https://doi.org/10.1016/j.jplph.2009.11.018.
73. Wolf, D.W., Henderson, D.W., Hsiao, T.C., & Alvino, A. (1988). Interactive water and nitrogen effects onsenescence of maize. I. Leaf area duration nitrogen distribution and yield. Agronomy Journal 80: 859-864. https://doi.org/2134/agronj1988.00021962008000060004x.
74. Yamauchi, M., Katayama, S., Todoroki, T., & Watanable, T. (1984). "Total synthesis of fulvic acid". Journal of the Chemical Society. Chemical Communications 23: 1565-6. https://doi.org/1039/C39840001565.
75. Youssef, A.A., Mahgoub, M.H., & Talaat, I.M. (2004). Physiological and biochemical aspects of Matthiola incana L. plants under the effect of putrescine and kinetin treatments. Egyptian Journal of Applied Science. 19(9B): 492-510.