Effect of Scale Position and Medium Type on Bulblet Production of Hippeastrum (Hippeastrum × johnsonii) with Twin Scaling Method

Document Type : Research Article

Authors

• Seyyedeh Mahdiyeh Kharrazi
• Ali Tehranifar
• Seyyed Hossein Nemati
• Abdolreza Bagheri

Ferdowsi University of Mashhad

https://doi.org/10.22067/jhorts4.v0i0.50883

Abstract

Introduction: Amaryllis is grown as pot outdoor plant and cut flower. Generally, this ornamental plant propagates by seed, suckers and scale cutting. Propagating by seed is not commercial and often used to produce new varieties. On the other hand, number of bulblets per mother bulb is very low under normal condition. Besides each bulb produces only 2 or 3 bulblets in a growing season and they become mature and produce flower stalk after 2 to 3 years. In some cases bulbs have no capacity to produce bulblet. Therefore, one of the strategies for shortening the growth period of the plant is to improve the traditional methods of plant propagations.
Materials and Methods: This study was done as a factorial experiment in a completely randomized design with 7 replications to evaluate the effects of medium and position of twin scales in mother bulbs on propagation of bulblets, in order to increase the rate of propagation of this ornamental plant. To measure wet and dry weight of explants, 3 replicates were used. For propagation, bulbs were cut radially into 12 equal pieces, so that each pieces were contained a part of the basal plate. To evaluate the effects of position of twin scales in mother bulbs, pieces were divided as twin scales and classified in 5 groups, so that the outermost twin scales was grouped in class 1 and the innermost twin scales was grouped in class 5. After that, the scale cuttings were dipped in 0.1 % carbendazim solution for 25 minutes and then surface water were dried using sterilized tissue paper. Media that used in this study were sand, perlite, vermiculite, Peat moss and cocopeat. For removing possible contamination from the media, all media were autoclaved for 30 minutes at 121 °C. Then twin scales cuttings were cultured in vented transparent plastic containers that filled with different media and were kept in a growth chamber at 25 °C and 16 hours lighting.Number of produced bulblet, bulblet diameter, root number, root length, fresh and dry weight of plants and browning rate of scales were recorded at the end of the experiment.
Results and Discussion: The results showed that medium and twin scale position in the mother bulb had a significant effect on the quality produced bulblet. The highest fresh weight of bulblet (1.58 g), bulblet dry weight (0.21 g) and the maximum diameter of the produced bulblet (1.5 cm) were obtained in the outermost twin scales and peat moss medium. Analysis of variance showed that the effect of culture medium on the number and length of produced leaf was significant (p

Keywords

• Bulblet diameter
• Cuttage
• Multiplication rate
• Peat moss

20.1001.1.20084730.1396.31.1.16.8

References

1- Andrade-Rodriguez M., Guillen-Sanchez D., Villegas-Torres O.G., Ayala-Hernandez J.J., Lopez-Martinez V., and Vargas-Araujo J. 2015. Bulb cutting methods to propagate Hippeastrum hybridum Hort. Revista Chapingo Serie Horticultura, 21:57-69.

2- Brown S.P., and Black R.J. 2007. Amaryllis, University of Florida, IFAS Extension.

3- Clemson Extension, 1995. Understanding and producing amaryllis. Clemson Extension. Horti. L 63.

4- De Bruyn M.H. 1997. Micropropagation of amaryllis (Hippeastrum×hybridum). In: Bajaj, Y.P.S. Biotechnology in Agriculture and Forestry 40, High-Tech and Micropropagation VI. Springer Verlag, Heidelberg, New York, pp 1-13.

5- De Gelder A. 1990. Variety evaluation of hippeastrum. Acta Horticulture, 226: 273-275.

6- Dohare S.R., 1989. Amaryllis and Hippeastrum. In: Commercial Flowers. T. K. Bose, R.G. Maiti, R.S. Dhva, (Eds.). Naya prokash, Calcutta, 573-593.

7- El-Naggar A.H., and El-Nasharty A.B. 2009. Effect of growing media and fertilization on growth, flowering, bulbs productivity and chemical constituents of Hippeastrum vittatum, Herb. American-Eurasian Journal of Agricultural & Environmental Sciences, 6:360-371.

8- Ephrath E., Ben-Asher J., Baruchin F., Alekperov C., Dayan E., and Silberbush M. 2001. Various cutting methods for the propagation of Hippeastrum bulbs. Biotronics, 30:75-83.

9- Fennell C.W., 2013. Crinum moorei: propagation and secondary metabolite production in vitro. University of KwaZulu-Natal. Thesis for the degree of Ph.D in Botany.

10- Huang C.W., Okubo, H., and Uemoto S. 1990a. Importance of two scales in propagating Hippeastrum hybridum by twin scaling. Scientia horticulturae, 42(1-2):141-149.

11- Huang C.W., Okubo, H., and Uemoto S. 1990b. Comparison of the bulblet formation from twin scales and single scales in Hippeastrum hybridum cultured in vitro. Scientia horticulturae, 42(1-2):151-160.

12- Hanks G. 2015. A review of production statistics for the cut flower and foliage sector 2015 (part of AHDB Horticulture funded project PO BOF 002a). The national cut flower center, AHDB.

13- Hubner S. 2014. International Statistics flowers And Plants 2014. Volume 62, center for Business Management in Horticulture and applied research, leibniz University Hanover, Germany.

14- Jamil M.K., Rahman M.M., and Rahman M.M. 2014. Effect of Bulb Cutting and Pot Medium on Propagation of Hippeastrum (Hippeastrum hybridum Hort.). Journal of Ornamental Plants, 4(3): 123-132.

15- Marinangeli P.A., Hernandez L.F., Pellegrini C.P., and Curvetto N.R. 2003. Bulblet Differentiation after Scale Propagation of Lilium longiflorum. Journal of the American Society for Horticultural Science, 128(3): 324-329. 2003.

16- Meerow A.W., Broscht T.K., and Kane M.E. 1991. Hippeastrum breeding at the University of Florida. Herberita, 47: 4-10.

17- Padasht M.N., Khalighi A., Naderi R., and Mousavi A. 2005. The investigation of different methods for culturing and propagation of Chelcheragh lily (Lilium ledebourii), native of Iran, and its introduction possibility as a new floricultural crop. Islamic Azad University, Science and Research Unit. Thesis for the degree of Ph.D in horticulture (in Persian with English abstract).

18- Sharifi A., Moshtaghi N., and Bagheri A. 1994. Applied Plant tissue culture, Jahad daneshgahi Mashhad publisher, Mashhad (in Persian).

19- Silberbush M., Ephrath J.E., Alekperov Ch., and Ben-Asher J. 2003. Nitrogen and potassium fertilization interactions with carbon dioxide enrichment in Hippeastrum bulb growth. Scientia Horticulturae, 98:85-90.

20- Smith R.H., Burrow J., and Kurten K. 1999. Challenges associated with micropropagation of Zephyranthes and Hippestarum sp. (Amaryllidaceae). In Vitro Cellular and Developmental Biology - Plant, 35(4): 281-282.

21- Tombolato A.F.C., Azevedo C., and Nagai V. 1994. Effects of Auxin Treatments on in Vivo Propagation of Hippeastrum hybridum Hort. by Twin Scaling. Hort science, 29(8):922.

22- Witomska M., Ilczuk A., and Zalewska I. 2005. Effect of cutting size on propagation efficiency of Hippeastrum × chemielii by scale cuttings. Propagation of Ornamental Plants, 4:205-209.

23- Yuan Y.L., Zhang Y.L., Zhao J.L., Zhao J. 2008. Study on the method of scale propagation of Amaryllis vittata. Journal of Northwest A & F University, Natural Science Edition, 36:108-112.

24- Zhang W., Song L., Teixeira da silva J.A., and Sun H. 2013. Effects of temperature, plant growth regulators and substrates and changes in carbohydrate content during bulblet formation by twin scale propagation in Hippeastrum vittatum Red Lion. Scienntia Horticulturae, 160:230-237.

25- Zhu Y., Lui K.S., and Yiu J.C. 2005. Effect of cutting method on bulb production of Hippeastrum hybridumin in Taiwan. Acta Horticulturae, 673: 531-535.