نوع مقاله : مقالات پژوهشی
نویسندگان
1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 دانشگاه علوم کشاورزی و منایع طبیعی گرگان
3 پژوهشکده گیاهان دارویی دانشگاه شهیدبهشتی
4 گرگان
چکیده
ایران جزو مناطق خشک و نیمه خشک جهان است و با کمبود منابع آب و وجود زمین های شور مواجه است. با توجه به افزایش جمعیت و محدودیت اراضی قابل کشت، استفاده از منابع آب و خاک شور کشور ضروری به نظر می رسد. یکی از روش های بهره برداری از این منابع کشت گیاهان دارویی است. اسطوخودوس (Lavandula angustifolia) گیاه دارویی چند ساله و چوبی است که با هدف تهیه اسانس موجود در گل ها و برگ هایش کشت می‌شود. به‌منظور بررسی اثر سطوح مختلف تنش شوری بر شاخص های رشد، عملکرد و ترکیب اسانس برگ گیاه اسطوخودوس، آزمایشی گلدانی بر پایه طرح کاملاً تصادفی با پنج تیمار و سه تکرار انجام گردید. تنش به صورت هیدروپونیک و در پنج سطح 0، 25، 50، 75 و 100 میلی‌مولار کلرورسدیم اعمال شده و پس از چهار ماه اندازه‌گیری شاخص های رشدی از قبیل طول ساقه، طول ریشه، وزن تر ساقه، وزن تر ریشه و وزن خشک ریشه، درصد و ترکیب اسانس برگ انجام پذیرفت. نتایج تجزیه های آماری و اندازه گیری با دستگاهGC-MS نشان دادند تنش شوری اثر معنی داری بر شاخص های رشد، درصد و ترکیب اسانس دارد .با افزایش شوری، طول ساقه، وزن تر ساقه و وزن تر ریشه و وزن خشک ریشه کاهش یافته و طول ریشه و درصد اسانس ابتدا تا سطح شوری 25 میلی‌مولار افزایش و سپس کاهش یافت. ترکیب اجزای اسانس تحت تاثیر تنش شوری، تغییر کرده است. به طوری که با افزایش سطح شوری مقدار بورنئول، کادینول، کامفور، کاریوفیلن اکساید و سیمن-8-ال ابتدا افزایش و سپس در سطح 100 میلی‌مولار شوری کاهش یافته است که مهم ترین ماده در ترکیب اسانس برگ اسطوخودوس، بورنئول می باشد که با افزایش سطح شوری مقدار آن افزایش قابل‌توجهی نشان داد که می توان نتیجه گرفت در سطوح اولیه تنش شوری درصد اسانس افزایش می یابد ولی با شدیدتر شدن تنش علی رغم کاهش در درصد اسانس تولید شده، کیفیت اسانس افزایش خواهد داشت.
کلیدواژه‌ها
عنوان مقاله [English]
The Effect of Salinity Stress on the Growth, quantity and quality of Essential oil of Lavender (Lavandula angustifulia Miller)
نویسندگان [English]
- Sara Khorasaninejad 1
- Hassan Soltanloo 2
- Javad Hadian 3
- Sadegh Atashi 4
1 Gorgan University of Agricultural Sciences and Natural Resources
2 Gorgan University of Agricultural Sciences and Natural Resources
3 Medicinal Plant Research Istitude of Shahid Beheshti University
4 Gorgan
چکیده [English]
Introduction: Plants are usually exposed to different environmental stresses which limit their growth and productivity as well as cause considerable loss of worldwide agricultural production. One of the most important factors affecting plant and production of secondary metabolites is the salt stress. Salinity of soil or water is one of major stress, obstacles to increase production in plant growing areas throughout the world and especially in arid and semi-arid regions it can severely limit plant production. Iran is among the world's arid and semi-arid land, and faces water resources shortage and saline lands. According to the concept of sustainable development and role of Agriculture, using saline water and soil resources seems mandatory. Recently, medicinal and aromatic plants have received much attention in several fields such as agroalimentary, perfumes, pharmaceutical industries and natural cosmetic products. Although, secondary metabolites in the medicinal and aromatic plants were fundamentally produced by genetic processing, but, their biosynthesis are strongly influenced by environmental factors. It means that biotic and abiotic environmental factors affect growth parameter, essential oil yield and constituents. Abiotic environmental stresses, especially salinity and drought have the most effect on medicinal plant. Medicinal plants cultivation is one of ways to exploit these resources. Essential oils help to easier adapt to the environmental stress conditions. Also, essential oils are not constantly in the quantitative and qualitative terms. They are changing continuously, due to the requirements of the environment, and to individual survival. The different results were dedicated from the effect of salinity stress on the quantitative and qualitative parameters. Lavender (Lavandula angustifulia Miller) is a perennial woody medicinal plant that cultivated for its an essential oil in leafs and flowers. Major parts of Lavender produces essential oil are flowers and leaves.
Materials and Methods: This experiment was carried out using a randomized complete block design with three replications to study the effect of salinity stress on growth parameters, essential oil constituents and yield of Lavender (Lavandula angustifulia) at the Horticultural Sciences Department, Plant Product faculty, Gorgan Agricultural Sciences and Natural Resources University. Lavender plants were obtained from seed plantation. The seeds in this investigation were obtained from the Institution of Forests and Range researches in Tehran. After three weeks stratification (4ºC) and germination, five plants were transplanted into similarized pots that were filled with perlite and cocopeat (2:1). Irrigation treatments with hydroponic solution were completed during germination until stage of 6-8 leaf. Then, five levels of salt stress, including 0, 25, 50, 75 and 100 mM NaCl levels were investigated during four months, applied in hydroponic. Length, shoot wet weight, root wet weight and root dry weight were measured at full flowering stage (after five months). The same time in order to evaluate percentage and composition essential oil, each plant were harvested and dried under room condition. After two weeks, Clevenger method was used to extract the essential oil from the plant foliage. The obtained essential oil were measured for calculating of essential oils percentage and then, analyzed by using GC/MS (Gas choromatography-mass spectrometry) for identification and quantification of the components. Statistical analysis of data was used with SAS software and charts preparing was done with Excel software. Mean comparison with LSD’s test in 5 percent probability was used.
Results and Discussion: Results indicated that salinity stress motivated a significant influence in all of the growth parameters and essential oil yield and percent in P < 0.05. Increasing salt of the soil led to reduce in stem length, shoot wet weight, root wet weight and root dry weight and increase length of root and leaf essential oil percent at first, and then led to reduce these two parameters.
The highest values of the essential oil percent were obtained from second level of salinity. Also, it was reported that salt stress had a significant effect on yield and percent of essential oil that was similar to our finding. This was reported that percent of essential oil in each plant and composition of essential oil changed. According to the results of this research, salinity treatment significantly increased essential oil percentage in Lavandula angustifulia.
Conclusion: The most important of leaf essential oil component is Borneol that was increased. So, the medium level of salinity stress caused to increase in percent and quality of lavender essential oil.
کلیدواژه‌ها [English]
- Borneol
- growth parameters
- GC-MS
- Hydroponic
- Medicinal plant
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