اثر محلول‌پاشی برگی اسید آسکوربیک و اسید اگزالیک بر پاسخ‌های فیزیولوژیک توت‌فرنگی رقم ’کاماروسا‘

نوع مقاله : مقالات پژوهشی

نویسندگان

گروه علوم باغبانی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

در سال­های اخیر کاربرد اسیدهای آلی به دلیل تأثیر آنها در افزایش عملکرد کمی و کیفی گیاه و افزایش مقاومت به تنش­های محیطی افزایش یافته است. به­همین منظور، برای بررسی برخی پاسخ­های فیزیولوژیک توت­فرنگی رقم ’کاماروسا‘ به محلول­پاشی برگی اسیدهای آلی، آزمایشی به‌صورت طرح بلوک­های کامل تصادفی در سه تکرار انجام شد. تیمارهای آزمایش شامل: محلول­پاشی با اسید آسکوربیک و اسید اگزالیک (هر کدام با غلظت یک میلی­مولار) در سه مرحله (از مرحله اتمام گلدهی تا مرحله میوه سبز) با فواصل شش روز یک‌بار و تیمار شاهد (عدم محلول­پاشی) بود. نتایج نشان داد که تیمار محلول­پاشی اثر معنی‌داری بر سطح برگ، وزن تر، وزن خشک برگ، میزان کلروفیل­های a، b و کل، کارتنوئید، قند کل، فلاونوئید و فنل کل داشت. بیشترین میانگین سطح برگ (08/314 سانتی­متر مربع)، وزن تر و خشک برگ (به‌ترتیب 78/1 و 56/0 گرم)، کلروفیل a و کارتنوئید کل (به‌ترتیب 43/0 و 29/0 میلی­گرم بر گرم)، قند کل (43/1 میکروگرم بر گرم)، فنل کل (70/0 میلی­گرم بر گرم) و فلاونوئید کل (19/0 میلی­گرم بر گرم) در کاربرد اسید اگزالیک مشاهده شد. تیمار شاهد دارای کمترین میانگین در تمام صفت­های مورد مطالعه (به جز کارتنوئید) بود. مصرف اسید آسکوربیک و اسید اگزالیک سبب افزایش رنگیزه­های فتوسنتزی شد و نسبت به تیمار شاهد به‌طور میانگین ۲۲ درصد میزان کلروفیل کل را افزایش داد. به­علاوه، محلول­پاشی با اسید آسکوربیک و اگزالیک باعث افزایش مواد جامد محلول، فعالیت آنتی­اکسیدانی و فلاونوئید میوه نسبت به شاهد شد. همچنین میوه­های برداشت شده از گیاهان تیمار شده با اسید اگزالیک، آنتوسیانین و فنل کل بیشتری نسبت به میوه­های برداشت شده از گیاهان تیمار شده با اسید آسکوربیک و شاهد دارا بودند. میوه­های گیاهان تیمار شده با اسید آسکوربیک نسبت به گیاهان تیمار شده با اسید اگزالیک، ویتامین ث و اسیدیته قابل تیتر کمتر و شاخص طعم بالاتری داشتند. به‌طور کلی با توجه به نتایج مشخص شد که محلول­پاشی برگی اسید آسکوربیک و اسید اگزالیک با افزایش رنگیزه­های فتوسنتزی و زیست­توده، باعث بهبود صفات کیفی و محتوای ترکیبات مرتبط با سلامتی در میوه توت­فرنگی رقم ’کاماروسا‘ شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Foliar Application of Ascorbic Acid and Oxalic Acid on the Physiological Responses of Strawberry cv. Camarosa

نویسندگان [English]

  • N. Soukht saraei
  • F. Varasteh
  • M. Alizadeh
Department of Horticultural Sciences, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

Introduction
In recent years, the use of organic acids has increased due to their role in the quantitative and qualitative yield and resistance to environmental stresses. Ascorbic acid (vitamin C) is one of the important antioxidants and plays a role as the primary substrate in cyclic pathways to remove toxicity and neutralize superoxide and single oxygen radicals. Ascorbate is also involved in the regulation of cell division and photosynthesis and has nutritional value for humans and is probably important for the tolerance of plants against photo-oxidative stress. Oxalic acid is a metabolic end product in plants that has many physiological functions, the main ones is the induction of resistance to disease and environmental stress by increasing the activity of enzymes involved in resistance and secondary metabolites such as phenol, flavonoid, etc. Considering the importance of the physiological traits of the plant in the production of quantitative and qualitative yield of strawberry and on the other hand, the lack of sufficient information about the effect of external application of ascorbic acid and oxalic acid on the physiological traits of the plant, the present research work aimed to investigate some physiological and qualitative traits of strawberry leaves and fruits affected by foliar spraying of ascorbic acid and oxalic acid.
 
Materials and Methods
The experiment was conducted based on randomized complete block design (RCBD) with three replications in Darkalate village of Ramiyan city of Golestan province to investigate some physiological responses of strawberry cv. Camarosa to the foliar application of organic acids. The experimental treatments consisted of three levels: non-spraying as control, spraying with 1 mM ascorbic acid and 1 mM oxalic acid, which was performed in three stages (from end flowering stage to the green fruit stage) at 6 days intervals. Finally, three plants were selected from each experimental unit and leaf samples were taken and transferred to the laboratory to measure physiological traits i.e. leaf area, fresh and dry weights, total chlorophylls, chlorophyll a and chlorophyll b, total carotenoids, total sugars, total phenols and flavonoids. Also, when at least 75 percent of the fruit surface turned red, the fruits were harvested from each plot separately and immediately transferred to the Plant Physiology Laboratory of Gorgan University of Agricultural Sciences and Natural Resources. The physicochemical traits of strawberry fruits including total soluble solids (TSS), titratable acidity (TA), flavor index, vitamin C, total phenol, flavonoid, antioxidant activity, total anthocyanins were measured. Analysis of data were performed using SAS 9.2 statistical software and comparison of mean data were undertaken based on LSD statistical test.
 
Results and discussion
The results showed that the foliar application of ascorbic acid and organic acid had a significant effect on the leaf area, fresh and dry weights, total chlorophylls, chlorophyll a, chlorophyll b, carotenoids, total sugars, phenols and flavonoids. The highest mean leaf area (314.08 cm2), leaf fresh and dry weights (1.78 and 0.56 grams, respectively), chlorophyll a (0.43 mg/g), total carotenoids (0.29 mg/g), total sugars (1.43 µg/g), total phenols (0.70 mg/g) and flavonoids (0.19 mg/g) were observed in the application of oxalic acid. The control had the lowest mean in all studied traits (except carotenoids). The application of both ascorbic acid and oxalic acid resulted in an increase in photosynthetic pigments, elevating the total chlorophyll content by an average of 22% compared to the control group. Additionally, foliar spraying with ascorbic and oxalic acid led to higher levels of total soluble solids, increased antioxidant activity, and enhanced total flavonoid content in the fruit compared to the control group.Moreover, fruits harvested from plants treated with oxalic acid before harvest exhibited higher levels of total anthocyanin and phenol compared to fruits from plants treated with ascorbic acid before harvest and the control group. Fruits of plants treated with ascorbic acid compared to plants treated with oxalic acid had lower vitamin C and titratable acidity and higher flavor index. The physiological influence of oxalic acid was more superior than that of ascorbic acid and had higher positive effects in the studied traits were recorded. However, since ascorbate is one of the precursors of oxalic acid biosynthesis. Therefore, the changes caused by oxalic acid are indirectly influenced by ascorbic acid. Ascorbic acid acts as a cofactor in photosynthetic reactions and prevents the destruction of chlorophylls and carotenoids due to its antioxidant property, and by interfering in cell division and increasing the surface area of leaves; it increases photosynthesis and the production of carbohydrates. In general, according to the results, it was found that the application of ascorbic acid and oxalic acid improved the quality characteristics and the content of health related compounds of Camarosa strawberry fruit by increasing photosynthetic pigments and biomass.

کلیدواژه‌ها [English]

  • Biomass
  • Organic acids
  • Photosynthetic pigments
  • Total phenol

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

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  • تاریخ دریافت: 25 بهمن 1401
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