Document Type : Research Article

Author

Assistant Professor, Greenhouse Cultivation Research Department, Tehran Agricultural and Natural Resources Research and Education Center, AREEO, Varamin, Iran

Abstract

Introduction: Garlic (Allium sativum L) is the second most commonly used herb of alliums after onions. This plant has significant effects on lowering blood pressure, preventing atherosclerosis, reducing blood cholesterol and triglyceride and inhibiting platelet aggregation. In recent years, the increase in the yield of this product has been of interest to manufacturers of this product with respect to the preservation of qualitative characteristics, including the maximum content of nitrate. It has been shown that the absorption of nutrients in the soil affects the yield and yield components of the plant. In this regard, the timely and adequate supply of nutrients such as sulfur and nitrogen has a special role in promoting quantitative and qualitative characteristics of garlic.
Material and Methods: In order to study the effects of nitrogen and sulfur consumption on quantitative and qualitative traits of garlic, this study was conducted with 12 treatments and three replications in the form of a split plot design in two years in Agricultural Research Center of Agricultural and Natural Resources of Tehran Province which located in Varamin in Iran. Nitrogen factor was considered at four levels (0, 100, 200 and 300 kg ha-1) and sulfur at three levels (0, 500 and 1000 kg ha-1 with inoculation of Thiobacillus). Before planting, soil samples were taken from the experimental site and physical and chemical characteristics including texture, reaction, organic carbon, lime, nitrogen, phosphorus and potassium, and iron, manganese, copper, zinc and boron were measured. Dry matter yield, number of bulbs per garlic, single plant weight, garlic weight, plant height, leaf number per plant and leaf area were recorded at the end of experiment. Garlic nitrate content and nutrient concentration in garlic leaves were also measured. Data obtained were analyzed statistically using SAS software.
Results and Discussion: The effects of nitrogen and sulfur on the yield of garlic cloves, number of bulbs in cloves, cloves weights, bulbs weights, plant height, leaf area and bulb nitrate were significant as all these traits were improved. Application of 1000 kg.ha-1 of sulfur increased the garlic cloves yield by 31% compared to the control. Nitrogen consumption up to 200 kg.ha-1 increased garlic cloves yield by 58% compared to the control. The highest economic yield of garlic cloves was obtained by 1000 kg.ha-1 sulfur and 200 kg.ha-1 nitrogen, which was 104% more than control treatment.
Data also showed that the effects of sulfur and nitrogen and the interaction of sulfur and nitrogen on the leaf area of the garlic plant were significant. Consumption of 1000 kg.ha-1of sulfur increased the leaf area of the garlic plant by 42% compared to the control. Nitrogen consumption up to 200 kg.ha-1 increased the leaf area of garlic plant by 39.3% compared to the control. The highest leaf area of garlic plant was obtained from 1000 kg.ha-1sulfur and 200 kg.ha-1nitrogen, which was 104% higher than control. Increase of the growth and yield of garlic was attributed to the main role of nitrogen in increasing meristematic activity, cell division, prolongation of the cell. Sulfur is one of the essential nutrients of the plant, and without sulfur, the plant does not grow and function properly. Sulfur is the fourth essential element after nitrogen, potassium and phosphorus for garlic. This element is needed in production of amine acids, such as methionine and cysteine, which are essential for the production of vitamin A, and the activation of certain enzymes. The results of studies have shown that amino acids directly or indirectly affect the physiological activity of the plant, and positively affect the growth, increase in yield and increase plant tolerance to environmental stresses. The soil reaction was affected by sulfur consumption and decreased. There was a negative correlation between soil reaction as a dependent variable (Y) and consumed sulfur as an independent variable (X), which correlated with linear relation with correlation coefficient of 0.98. Data showed that use of 1000 kg.ha-1of sulfur reduced soil reaction from 7.49 to 7.26 and increased leaf iron concentration by 88% compared to control and increased leaf zinc concentration 110% compared to control treatment. The researchers believe that sulfur oxidation and sulfuric acid production in the soil reduce soil reactions and increase the absorption capacity of phosphorus and microelements. The effect of sulfur on the concentration of leaf phosphorus was significant. The phosphorus concentration was increased significantly with sulfur application into the soil, so that 1,000 kg.ha-1 of sulfur increased the concentration of leaf phosphorus by 63% compared with the control (0.33%). Research results have shown that phosphorus absorption capacity in soil is highly dependent on soil reaction and reducing soil reaction leads to increased phosphorus absorption capacity. Researchers have reported that sulfur consumption and the production of sulfuric acid, as a result of its oxidation, reduce soil reaction and increase phosphorus availability for plants. The effect of nitrogen, sulfur and the interaction of nitrogen and sulfur on the content of garlic nitrate was significant. Garlic nitrate content decreased with application of sulfur and increased with nitrogen addition. Application of sulfur at 1000 kg.ha-1 significantly reduced garlic nitrate content by 35% compared to control. The application of 100, 200 and 300 kg.ha-1 of nitrogen increased the content of garlic nitrate 97, 210 and 308%, respectively in comparison to control. At all levels of nitrogen addition, sulfur consumption reduced the content of garlic nitrate. Garlic nitrate content decreased with application of sulfur and increased with nitrogen addition.  Sulfur application at 1000 mg.kg-1 significantly reduced garlic nitrate content by 35% compared to control (mg.kg-1). Garlic nitrate was increased by the amount of nitrogen consumed. There was a positive correlation between garlic nitrate (Y) as a dependent variable (N) and independent nitrogen (X), which correlated with linear relation with the correlation coefficient of 0.99. Researchers have argued that excessive consumption of nitrogen fertilizers is the main factor causing the accumulation of nitrate in a number of vegetables and other plants such as garlic.
Conclusion: Based on the results of the experiment, it was concluded that nitrogen and sulfur increase the yield of garlic. Meanwhile, sulfur reduces nitrate accumulation in garlic. Sulfur consumption reduces soil pH and increases the absorption availability of micronutrients and phosphorus in the soil and causes more absorption of these elements by the plant.

Keywords

1-         Abdel-Fattah A., Rasheed M.A., and Shafei A.M. 2005. Phosphorus availability as influenced by different application rates of elemental sulfur to soils. Egyptian Journal of Soil Science 45(2): 199–208.
2-         Adem B.E., and Tadesse S.T. 2014. Evaluating the role of nitrogen and phosphorous on the growth performance of garlic (Allium sativum L.). Asian Journal of Agricultural Research 8: 211–217
3-         Akhavan Z., and Fallah Nosrat Abad A.R. 2013. The effect of sulfur and Thiobacillus inoculants on soil pH, dry matter weight and phosphorus absorption canola. Journal of Soil Management for Sustainable Agriculture 3: 1-13. (In Persian with Persian abstract)
4-         Aliehyaie M. 1996. Methods of chemical analysis of soil.Vol: 2, Thecnical report No: 1024, Soil and Water Research Institute, Tehran, Iran. (In Persian)
5-         Aulakh M.S. 2003. Crop response to sulfur nutrition. In: Sulfur in Plants. [eds. Y.P. Abrol and A. Ahmed]. Kluwer Academic Publication Dordrecht 341-354
6-         Balwan S., Duhan B.S., Yadav H.D., and Kumar V. 2006. Effect of different factors on oxidation of elemental sulfur in soils of Haryana. Haryana Agriculture University Journal Research 36: 31–34.
7-         Barker A.V., and Pilbeam D.J. 2006. Handbook of plant nutrition. CRC Press. pp: 196.
8-         Besharati H. 2017. Effects of sulfur application and Thiobacillus inoculation on soil nutrient availability, wheat yield and plant nutrient concentration in calcareous soils with different calcium carbonate content. Journal of Plant Nutrition 40: 447-456.
9-         Chien S.H., Gearhart M.M., and Villagarcía S. 2011. Comparison of ammonium sulfate with other nitrogen and sulfur fertilizers in increasing crop production and minimizing environmental impact: a review. Soil Science 176 (7): 327-335.
10-     Cui Yanshan., Dong Y.T., Li H.F., and Wang Q.G. 2004. Effect of elemental sulfur on solubility of soil heavy metals and their uptake by maize. Environment International 30(3): 323-328.
11-     Delfin S., Tognetti R., Dsiderio E., and Alvino A. 2005. Effect of foliar application of N and humic acids on growth and yield of durum wheat.Agronomy of Sustainable Development 25: 183-191.
12-     Diriba-Shiferaw G., Nigussie-Dechassa R., Woldetsadik K., Tabor G., and Sharma J.J. 2015. Effect of nitrogen, phosphorus, and sulfur fertilizers on growth, yield, and economic returns of garlic (Allium Sativum L.). African Journal of Agricultural Research 4(2): 10–22.
13-     Ebrahimi M.H., Sharafzadeh M., and Bazrafshan F. 2014. The influence of nitrogen levels on growth and bulb yield of two garlic cultivars. European Journal of Experimental Biology 4(1): 270-272
14-     El-Kholy A.M., Ali O.M., El-Sikhry E.M., and Mohamed A.I. 2013. Effect of sulfur application on the availability of some nutrients in Egyptian soils. Egyptian Journal of Soil Science 53(3): 361-377
15-     El-Zohiri S.S.M., and Abdou Y.M. 2009. Response of garlic plants to nitrogen levels and some growth stimulant. Annals of Agricultural Science 47(3): 361-374.
16-     Eriksen J. 2009. Soil sulfur cycling in temperate agricultural systems. Advance Agronomy 102: 55–89.
17-     Farooqui M.A., Naruka I.S., Rathore S.S., Singh P.P., and Shaktawat R.P.S. 2009. Effect of nitrogen and sulfur levels on growth and yield of garlic (Allium sativum L.)”. Asian Journal of Food and Agro-Industry. Special Issue.
18-     Fazili I.S., Jamal A., Ahmad S., Masoodi M., Khan J.S., and Abdin M.Z. 2008. Interactive effect of sulfur and nitrogen on nitrogen accumulation and harvest in oilseed crops differing in nitrogen assimilation potential. Journal of Plant Nutrition 31(7): 1203-1220.
19-     Getu S. 2015. Assessment of garlic production practices and effects of different rates of NPS fertilizer on yield and yield components of Garlic (Allium Sativum L.) under irrigated farming system in Yilmana Densa district, Amhara Region, Ethiopia MSc Thesis. Bahir Dar University.
20-     Hammad S.A., El-Hamdi Kh. H., Abou El-Soud M.A. and El-Sanat G.M.A. 2007. Effect of some soil amendments application on the productivity of wheat and soybean, mobility and availability of nitrogen. Journal of Agriculture Science Mansoura University 32(9): 7953–7965.
21-     Havlin J.L., Beaton J.D., Tisdale S.L., and Nelson W.L. 2004. Soil fertility and fertilizers. An introduction to nutrient management. 7th edn. Person Education Inc. Singapore. pp. 221
22-     Hore J.K., and Chanchan M. 2003. Influence of nitrogen and sulfur nutrition on growth and yield of garlic (Allium sativum L.). Journal of Crop and Weed. 10: 14–18.
23-     Hu H., Spark D., and Evan J.J. 1991. Sulfur deficiency influences vegetative growth, chlorophyll and element concentration s, and amino acids of pecan. Journal of American Society of Horticalture Science 116: 974–980.
24-     Hore J.K., Ghanti S., and Chanchan M. 2014. Influence of nitrogen and sulfur nutrition on growth and yield of garlic (Allium sativum L.). Journal of Crop and Weed 10(2): 14-18.
25-     Hsu J., Arcot J., and Lee A. 2009. Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary intake in Australians. Food Chemistry 115:334–339.
26-     Imen A., Najjaa H., and Neffati M. 2013. Influence of sulfur fertilization on S-containing, phenolic, and carbohydrate metabolites in rosy garlic (Allium roseum L.): A wild edible species in North Africa. European Food Research Technology 237: 521-527.
27-     Kaker A.A., Abdullahzai M.K.; Saleem M., and Qaim Shah S.A. 2002. Effect of nitro-genous fertilizer on growth and yield of garlic. Asian Journal of Plant Sciences 1(5): 544-545.
28-     Karimizarchi M., Aminuddin H., Khanif M.Y., and Radziah O. 2013. Elemental sulfur oxidation rate in a Malaysian high pH soil. In: Soil Science Conference of Malaysia. K. Wan Rasidah (eds). pp. 39-43. Pahang: Malaysian Society of Soil Science.
29-     Karimizarchi M., Aminuddin H., Khanif M.Y., and Radziah O. 2014. Elemental Sulfur Application Effects on Nutrient Availability and Sweet Maize (Zea mays L.) Response in a high pH Soil of Malaysia. Malaysian Journal of Soil Science 18: 75-86
30-     Khan A.A., Zubair M., Bari A., and Maula F. 2007. Response of onion (Allium cepa L.) growth and yield to different levels of nitrogen and zinc in swat valley. Sarhad Journal of Agriculture 23(4): 933.
31-     Khavazi K., Nougholipour F., and Malakouti M.J. 2001. Effect of Thiobacillus and phosphate solubilizing bacteria on increasing P availability from rock phosphate for corn. International Meeting on Direcct Application of Rock phosphate and related Technology. Kuala Lumpur. Malaysia.
32-     Kilgore M.J., Magahi M.D., and Yakubu A.I. 2007. Productivity of two garlic (Allium sativum L.) cultivars as affected by different levels of nitrogen and phosphorous fertilizers in Sokoto, Nigeria. American – Eurasian Journal of Environmental Science 2(2): 158-162.
33-     Klikocka H. 2011. The effect of sulfur kind and dose on content and uptake of micro-nutrients by potato tubers (Solanum tubersosum L.). Acta Scientiarum Polonorum Hortorum Cultus 10(2): 137-151.
34-     Lindsay W.L. 1979. Chemical Equilibriums in Soils. New York. John Wiley and Sons Ltd.
35-     Magray M.M., Chattoo M.A., Narayan S., and Mir S.A. 2017. Influence of Sulfur and Potassium Applications on Yield, Uptake & Economics of Production of Garlic, International Journal of Pure and Applied Bioscience 5(5): 924-934
36-     Martins N., Petropoulos S., and Ferreira I.C.F.R. 2016. Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre- and post-harvest conditions: A review. Food Chemical 211: 41-50.
37-     Mashhadi Jafarlou A. 2006. Effect of Irrigation frequency and different Levels of nitrogen and sulfur on garlic Yield. Master's Thesis. Department of Soil Science. Zanjan University.
38-     Mayer J.E., Pfeiffer W.H., and P Beyer. 2008. Biofortified crops to alleviate micronutrient malnutrition. Current Opinion in Plant Biology 11(2): 166-170.
39-     Menesatti P., Antonucci F., Pallottino F., and Intrigliolo F. 2010. Estimation of plant nutritional status by Vis-NIR spectropHotometric analysis on orange leaves (Citrus sinensis). Jounal of Agricultural Engenearing Reserch 105: 448-54.
40-     Mohammadi Aria M., Lakzian A., Haghnia G.H., Berenji A.R., Besharati H., and Fotovat A. 2010. Effect of Thiobacillus, sulfur and vermicompost on the water-soluble phosphorous of hard rock phosphate. Bioresource Technology 101: 551-554
41-     Mostafa M.A., El-Gala A.M., Wassif M., El Maghraby S.E., and Hilal M.H. 1990. Distribation of some micronutrients through a calcareous soil columns under sulfur and saline water application. Proceedings Middle East Sulfur Symposium. 12–16 February. Cairo. Egypt. pp 263–276.
42-     Motior M.R., Abdou A.S., Fareed H.A.D., and Sofian M.A. 2011. Responses of sulfur, nitrogen and irrigation water on Zea mays growth and nutrients uptake. Australian Journal of Crop Science 5(3): 347-357.
43-     Mulatu A., Tesfaye B., and Getachew E. 2014. Growth and bulb yield garlic varieties affected by nitrogen and phosphorus application at Masan Worde, South Central Ethiopia. Sky Journal of Agricultural Research 3: 249–255.
44-     Nasef I.N., and Elwan M.W.M. 2016. Response of Yield and Quality of Garlic to Nitrogen Sources and Foliar Spray with Sulfur Treatments.  Journal of Plant Production. Mansoura University 7(12): 112-119
45-     Nasreen S., Haque M.M., Hossain M.A., and Fadid A.T.M. 2007. Nutrient uptake and yield of onion as influenced by nitrogen and sulfur fertilization. Bangladesh Journal of Agricultural Research. 32(3): 413-420.
46-     Nouri M., and Bayat F. 2016. Changes in Vegetative Growth Indicators and Garlic Performance in Nitrogen Fertilizer Sources and Levels. Two Seasons Letters of vegetables. Ilam University.
47-     Pavlou G.C., and Ehaliotis C. 2007. Effect of organic and inorganic fertilizers applied during successive crop season on growth and nitrite accumulation in lettuce. Scientia Horticulturae 111(4): 319-325.
48-     Safaa M M., Khaled S.M., and Hanan S. 2013. Effect of elemental sulfur on solubility of soil nutrients and soil heavy metals and their uptake by maize plants. Journal of American Science 9(12): 19-24.
49-     Salimpour S., Khavazi K., Nadian H., Besharati H., and Miransari M. 2010. Enhancing phosphorus availability to canola (Brassica napus L.) using P solubilizing and sulfur oxidizing bacteria. Australian Journal of Crop Science 4: 330-334.
50-     Salimpour S., Khavazi K., Nadian H., Besharati H., and Miransari M. 2012. Canola oil production and nutrient uptake as affected by phosphate solubilizing and sulfur oxidizing bacteria. Journal of Plant Nutrition 35: 1997-2008.
51-     Sameni A.M., and Kasraian A. 2004. Effect of agricultural sulfur on characteristics of different calcareous soils from dry regions of Iran. I. Disintegration rate of agricultural sulfur and its effects on chemical properties of the soils. Communications in Soil Science and Plant Analysis 35(9-10): 1219-1234.
52-     Sebnie W., Mengesha M., Girmay G., and Tesfaye Feyisa T. 2018. Response of garlic (Allium sativum L.) to nitrogen and phosphorus under irrigation in Lasta district of Amhara Region, Ethiopia. Cogent Food and Agriculture 4: 1532862
53-     Skwierawska M., Zawartka L., Skwierawski A., and Nogalska A. 2012. The effect of different sulfur doses and forms on changes of soil heavy metals. Plant, Soil and Environment 58:135-140.
54-     Susana C.B., Johannes L., Dawit S., Eduardo F.C., and Luís R.F.A. 2013 Sulfur forms in organic substrates affecting S mineralization in soil. Geoderma 156–164.
55-     Vidyalakshmi R., Paranthaman R., and Bhakyaraj R. 2009. Sulfur oxidizing bacteria and pulse nutrition-A review. World Journal of Agricultural Sciences 5(3): 270-278
56-     Ye R., Wright A.L., and McCray J.M. 2011. Seasonal changes in nutrient availability for sulfur -amended everglades soils under sugarcane. Journal of Plant Nutrition 34(14): 2095-2113.
57-     Zaki H.E.M., Toney H.S.H., and Abd Elraouf R.M. 2014. Response of two garlic cultivars (Allium sativum L.) to inorganic and organic fertilization. Nature and Science 12(10): 52-60.
58-     Zapata F., and Roy R.N. 2004. Use of phosphate rocks for sustainable agriculture. Publication of the FAO Land and Water Development Division 117-122.
59-     Zhou Y., Haneklaus S., Singh B.R., and Schnug E. 2008. Effect of repeated applications of elemental sulfur on microbial population, sulfate concentration, and pH in soils. Communications in Soil Science and Plant Analysis 39: 124–140.
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