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امیر حسین کشتکار نوشین فلاحی محمد رضا عبدالهی حسن ساریخانی هوشمند صفری ژاله محسنی عراقی

چکیده

القاء پلي‌پلوئيدي با استفاده از مواد شيميايي جهش‌زا يکي از روش‌هاي به‌نژادی گياهان دارويي به منظور افزايش قابليت توليد متابوليت‌هاي ثانويه است. کلشيسين موثرترين ماده شيميائي جهش‌زا در القا پلي‌پلوئيدي گياهان مي‌باشد. به منظور بررسي تاثير تيمار کلشيسين بر القاء پلي‌پلوئيدي در گياه دارويي شنبليله (Trigonella foenum-graecum) پژوهشی به صورت فاکتوريل در قالب طرح کاملاً تصادفي با سه فاکتور کلشيسين (صفر، 05/0، 1/0، 2/0، 5/0 درصد وزن به حجم)، زمان (12، 24،  48 و 72 ساعت) و نمونه (بذر، مريستم ريشه و مريستم انتهايي) در 3 تکرار انجام شد. در این آزمايش اثر کلشيسين بر درصد صفات زنده­ماني، ميکسوپلوئيدي و تتراپلوئيدي در نمونه‌هاي بذر، جوانه انتهايي و ريشه ارزیابی شد. بررسی‌ها نشان داد که بعد از تيمار شاهد بیشترین درصد زنده­ماني مربوط به غلظت 05/0 درصد کلشيسين براي نمونه جوانه انتهايي است و بالاترين درصد ميکسوپلوئيدي مربوط به نمونه جوانه انتهايي با غلظت تيمار 1/0 و 2/0 درصد کلشيسين می­باشد. نتایج بررسی­های مورفولوژيکي، سيتوژنتيکي، فلوسايتومتري و بيوشيميايي نشان داد که نمونه جوانه انتهايي در غلظت 5/0 درصد کلشيسين در مدت زمان 72 ساعت موثرترين تيمار در القاء تتراپلوئيدي در گیاه شنبلیله است. همچنين نتايج حاصل از  GC/MSبيانگر افزايش متابوليت­هاي ثانويه در گياه تتراپلوئيد نسبت به گياه ديپلوئيد شنبليله بود.

جزئیات مقاله

کلمات کلیدی

تتراپلوئیدی, فلوسایتومتری, کروماتوگرافی گازی توده‌ای, میکسوپلوئیدی

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ارجاع به مقاله
کشتکارا. ح., فلاحین., عبدالهیم. ر., ساریخانیح., صفریه., & محسنی عراقیژ. (2019). بررسی اثر کلشیسین بر القاء پلی‌پلوئیدی و اثرات آن بر ویژگی‌های مورفولوژیکی و بیوشیمیایی شنبلیله. علوم باغبانی, 33(1), 127-139. https://doi.org/10.22067/jhorts4.v33i1.71781
نوع مقاله
علمی - پژوهشی