The Effect of Trichoderma Fungus on the Morphophysiological Traits of the Basil Medicinal Plant (Ocimum basilicum L.) under Water Stress Conditions

Document Type : Research Article

Authors

• Mina Amani ¹
• Saeideh Alizadeh Salteh ²
• Mohsen Sabzi-Nojadeh ³
• Mehdi Younessi Hamzekhanlu ²

¹ Department of Horticultural Science and Engineering, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

² Department of Horticultural Science and Engineering, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran

³ Associate Professor, Department of Horticultural Science and Engineering, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran.

https://doi.org/10.22067/jhs.2023.80813.1231

Abstract

Introduction
Medicinal plants have long had a special place in the traditional ‎agricultural system of Iran and the use of these plants as medicine to prevent and treat diseases has been considered by ‎traditional medicine experts since ancient times. Medicinal plants with rich sources of secondary metabolites provide the ‎basic active ingredients of many medicines. Although the biosynthesis of secondary metabolites is genetically controlled, ‎but their construction is strongly influenced by environmental factors. One of the important climatic factors that affect the ‎distribution of plants around the world and can cause morphological, physiological and biochemical changes in the plant is ‎the lack of available water. Basil (Ocimum basilicum L.) seems to show little resistance to water stress. For this reason, there is a need for protective mechanisms for the ‎basil plant against stress due to water shortage. Plants are able to reduce or eliminate the effects of water shortage ‎stress by coexisting with a number of soil microorganisms. ‎Coexistence relationship plant with T. harzianum fungi is one of the ways to reduce dehydration ‎in plants. These mushrooms by altering some of the root properties and absorbing nutrients in the host plants, they reduce ‎the destructive effects of water shortage stress. Observing the positive effect of these fungi in increasing the absorption of nutrients from the soil, improving plant water relations, increasing water use efficiency in plants and finally increasing plant resistance to water deficit stresses on the one hand and on the other hand. The existence of water crises in different countries has prompted researchers to further study this aspect of the symbiotic relationship between the host plant and Trichoderma harzianum. ‎
 
Materials and Methods
Present study was performed to investigate the effect of T. harzianum on the antioxidant content of Ocimum basilicum under water deficit stress. It is also intended to evaluate the effects of an endophytic fungi namely T. harzianum on the shoot yield, photosynthetic pigments, content and yield of essential oil from O. ‎basilicum under water deficit stress. All experiments were performed as the factorial based on completely randomized design blocks with three replications in greenhouse condition. The experimental factors were the different irrigation regimes including 100, 75, 50 and 25% of field capacity and two concentrations of the T. harzianum (10⁶ and 10⁹ CFU/ml). At the beginning of flowering, water deficit stress was applied at four levels of 25, 50, 75 and the control treatment (100 percent) of field capacity until three weeks later. The application of water stress was such that the pots were weighed daily and the moisture deficiency in each treatment was removed by watering the pots until reaching the desired treatment level. After applying the stress, sampling and measuring traits were done at the full flower stage. After the plants reach the full flowering period, various traits including plant height, number of leaves, fresh and dry weight yield of the plant (as the economic yield of basil) and fresh and dry weight yield of roots per square meter, chlorophyll a, b, total (T), carotenoid, colonization percentage, yield and essential oil content were measured in all the plants in the pots.
 
Results and Discussion
Increasing the level of water stress reduced the economic performance of basil (O. basilicum). Coexistence with Trichoderma reduced the destructive effects of dehydration on the plant. With the application of dehydration stress, the essential oil content increased in mild and moderate stresses, but the yield of essential oil did not differ significantly from the control and plant height, number of leaves and plant pigments decreased under dehydration stress. According to the experimental results, it can be said that Trichoderma with a population density of 106 (spores per ml of inoculum) had a better effect on most growth indices, while the effect Trichoderma with a population density of 109 (spores per milliliter of inoculum) on important traits such as shoot fresh weight yield (212.2 g), percentage (0.7%) and essential oil yield It was higher. According to the obtained results, it can be stated that inoculation of basil with Trichoderma increased the percentage and yield of essential oil in both water stress and non-stress conditions, and considering that in cultivation of plants the goal is to increase the effective substance. It is in these plants.
 
Conclusion
Finally, the results indicated that the use of Trichoderma mushroom in comparison with the control (without inoculation with the fungus) under water stress conditions has the ability to improve plant growth and leads to an increase in plant efficiency under water stress conditions.

Keywords

• Abiotic stress
• Aerial part performance
• Essential oil
• Morphological traits

Main Subjects

• Medicinal Plants

©2023 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.

References

1. Ahmad, M., Zaffer, G., Razvi, S.M., Dar, Z.A., Mir, S.D., Bukhari, S.A., & Habib, M. (2014). Resilience of cereal crops to abiotic stress: a review. African Journal of Biotechnology, 13, 121-130. https://doi.org/5897/AJB2013.13532
2. Amani, M., Sabzi-Nojadeh, M., Alizadeh-Salteh, S., & Younessi Hamzekhanlu, M. (2023). Study of the effect of water stress and different species of Arbuscular Mycorrhizal Fungi on ‎economic yield, essential oil ‎yield and photosynthetic pigments of basil (Ocimum basilicum)‎. Journal of Agricultural Science and Sustainable Production. https://doi.org/10.22034/SAPS.2022.50909.2854
3. Amini, R., Ebrahimi, A., & Nasab, A.D.M. (2020). Moldavian balm (Dracocephalum moldavica) essential oil content and composition as affected by sustainable weed management treatments. Industrial Crops and Products, 150, 112416. https://doi.org/10.1016/j.indcrop.2020.112416
4. Aslani, Z., Hassani, A., Rasouli Sadeghiani, M.H., Sefidkan, F., & Brin, M. (2011). The effect of two species of Arbuscular mycorrhiza fungi (Glumus mosseae) and ( intraradices) on growth, chlorophyll content and phosphorus uptake in basil (Ocimum basilicum L.) under drought stress. Scientific-Research of Iranian Medicinal and Aromatic Plants, 27, 471-486.
5. Ganji Moghadam, E., Khodaei, S., & Zamanipour, M. (2023). Effect of three species of arbuscular mycorrhizal fungi on some physiological and growth characteristics of peach seedlings under drought stress conditions. Journal of Horticultural Science. (In Persian with English abstract) https://doi.org/10.22067/jhs.2023.79317.1202
6. Haq, T.U., Ali, A., Nadeem, S., Maqbool, M.M., & Ibrahim, M. (2014). Performance of canola cultivars under drought stress induced by withholding irrigation at different growth stages. Soil Environment, 33, 43-50. ‏
7. Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2, 43-56. https://doi.org/10.1038/nrmicro797
8. Hermosa, R., Viterbo, A., Chet, I., & Monte, E. (2012). Plant-beneficial effects of Trichoderma and of its genes. Microbiology, 158, 17-25. https://doi.org/10.1099/mic.0.052274-0
9. Hsiao, T.C. (1973). Plant responses to water stress. Annual Review of Plant Physiology, 24, 519-570.
10. Jaleel, C.A., Gopi, B., Sankar, P., Manivannan, A., Kishorekumar, R. S., & Panneers, L. (2007). Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedling under salt stress. South African Journal of Botany, 73, 190-195. https://doi.org/10.1016/j.sajb.2006.11.001
11. Kapoor, R., Chaudhary, V., & Bhatnagar, A.K. (2007). Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua Mycorrhiza, 17, 581-587. https://doi.org/10.1007/s00572-007-0135-4
12. Karimzadeh Asl, K., Ghorbanpour, M., Marefatzadeh Khameneh, M., & Hatami, M. (2018). Influence of drought stress, biofertilizers and zeolite on morphological traits and essential oil constituents in Dracocephalum moldavica Journal of Medicinal Plants, 17, 91-112. (In Persian with English abstract).
13. Khakdan, F., & Javanmard, A.S. (2022). The impact of drought stress on antioxidant activities of basil (Ocimum basilicum) cultivars extracts. Nova Biologica Reperta, 9, 200-212.
14. Khalil, S.E., & El-Noemani, A.S.A. (2015). Effect of bio-fertilizers on growth, yield, water relations, photosynthetic pigments and carbohydrates contents of Origanum vulgare plants grown under water stress conditions. American-Eurasian Journal of Sustainable Agriculture,9, 60-73. ‏
15. Margaret, A.O., James, M., Lynch, N., & John, M.W. (1994). Potential of Trichoderma As consistent plant growth stimulators. Biological Fertilizers and Soils, 17, 85-90. https://doi.org/10.1007/BF00337738
16. Mazhabi, M., Nemati, H., Rouhani, H., Tehranifar, A., Moghadam, E.M., Kaveh, H., & Rezaee, A. (2011). The effect of Trichoderma on polianthes qualitative and quantitative properties. Journal of Animal and Plant Sciences, 21, 617-621. (In Persian with English abstract)
17. Mohsenzadeh, F., Zafari, D., & Nouri Safa, B. (2016). Instability of some species of Trichoderma fungi to oil pollution. Journal of Cellular and Molecular Research (Iranian Journal of Biology), 29, 321-330. (In Persian with English abstract)
18. Nabizadeh, E., Haghshenas, M., Dolatmand, N., & Ahmadi, K. (2023). The effect of Mycorrhizal Fungus (Piriformospora indica) on the morphological, physiological and biochemical traits of the medicinal plant Stevia (Stevia rebaudiana) under drought stress. Journal of Horticultural Science, 37(2), 37(2), 453-465. (In Persian with English abstract). https://doi.org/10.22067/jhs.2022.75337.1173
19. Omidbaigi, R., Hassani, A., & Sefidkon, F. (2003). Essential oil content and composition of sweet basil (Ocimum basilicum) at different irrigation regimes. Journal of Essential Oil-Bearing Plants, 6: 104-108. (In Persian with English abstract). https://doi.org/10.1080/0972-060X.2003.10643335
20. Porra, RJ. (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls and Photosynthesis Research, 73, 149-156. https://doi.org/10.1023/A:1020470224740
21. Rahimi , Talei A., & Ranjbar M. (2019). The effect of drought stress on biochemical changes of peppermint, Iranian Journal of Crop Science, 50(2), 59-75. (In Persian). https://doi.org/10.22059/ijfcs.2018.239868.654367
22. Rajaeian, S., Ehsanpour, A.A., & Toghyani, M.A. (2015). Changes in phenolic compound, TAL, PAL activity of Nicotiana rustica triggered by ethanolamine pretreatment under in vitro salt stress condition. Iranian Journal of Plant Biology, 7, 1-12. https://doi.org/20.1001.1.20088264.1394.7.26.2.4
23. Seghatoleslami, M. (2013). Effect of water stress, bio-fertilizer and manureon seed and essential oil yield and some morphological traits of cumin. Bulgarian Journal of Agricultural Science, 19, 1268-1274. (In Persian with English abstract)
24. Shaalan, M.N. (2005). Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis). Egypt Journal of Agriculture Research, 83, 271-281. https://doi.org/10.21608/ejar.2005.242762
25. Sharma, KD., & Kuhad, MS. (2006). Influence of potassium level and soil moisture regime on biochemical metabolites of Brassica Brassica Journal, 8, 71-74.
26. Shubhra, K., Dayal, J., Goswami, C.L., & Munjal, R. (2004). Effects of waterdeficit on oil of Calendula aerial parts. Biologia Plantarum, 48, 445-448.
27. Shukla, N., Awasthi, R.P., Rawat, L., & Kumar, J. (2012). Biochemical and physiological responses of rice (Oryza sativa ) as influenced by Trichoderma harzianum under drought stress. Plant Physiology and Biochemistry, 54, 78-88.
28. Tajali, A.A., Amin, G., Chaichi, M.R., & Zahedi, G. (2007). Habitat influence on essential oil of Camphorosma monspeliaca in Iran. Asian Journal of Plant Sciences, 6, 1297-1299.
29. Yang, C., Zhang, Z., Gao, H., Liu, M., & Fan, X. (2014). Mechanisms by which the infection of Sclerotinia sclerotiorum (Lib.) de Baryaffects the photosynthetic performance in tobacco leaves. BMC Plant Biology,14, 1-11. https://doi.org/10.1186/s12870-014-0240-4