Document Type : Research Article

Authors

1 Associate Professor, Department of Horticulture, College of Agriculture, Isfahan University of Technology

2 Ph.D. Student, Department of Horticulture, College of Agriculture, Isfahan University of Technology

Abstract

Introduction: Plants are constantly faced with abiotic and biotic stresses during their whole life. Abiotic stresses are various adverse environmental factors, including drought, high salinity, heavy metals, cold or heat shock, and ozone. Resulting in dehydration and osmotic stress, drought has caused a dramatic reduction in crop production globally. Grafting can reduce the content of Malondialdehyde (MDA); prevent the accumulation of reactive oxygen species (ROS); increase activities of antioxidant enzymes; and maintain fresh and dry weights, grain yield, and relative water content in a variety of plants in response to drought stress. On the other hand, a range of abiotic and biotic elicitors can confer tolerance to drought stress in plants. Grafting of herbaceous fruit vegetables can reduce detrimental effects of biotic and abiotic challenges and cultural practices. Grafting can increase yield of cucurbits, initiate shoot growth, aid in resistance against nematodes and viruses, withstand high and low temperatures, improve nutrient and water absorption, resist against high concentration of salt, drought and waterlogging stresses. Grafting elite commercial cultivars onto selected vigorous rootstocks is a special method of adapting plants to counteract environmental stresses. Grafting is currently regarded as a rapid alternative tool to the relatively slow breeding methodology for increasing the environmental-stress tolerance of fruiting vegetables. Potential approach to reduce losses in production and improve water use efficiency under drought conditions in high-yielding genotypes would be to graft these varieties onto proper rootstocks capable of reducing the effect of water stress on the shoot and to increase tolerance to abiotic stresses. Cucumber (Cucumis sativus L.) is one of the main greenhouse vegetable crops widely grown in Saudi Arabia. The total greenhouse area for cucumber production in 2013 was 2605 hectares produced 236,087 tons. Major factor influencing growth and yield of cucumber is water quantity. The effects of different rootstocks on plant growth, yield, fruit quality and water consumption in cucumber was studied. The highest yield was obtained from 9075 (19.02 kg m2), which was 24.5 and 23.5% higher than in the non-grafted and self-grafted treatments, respectively. The plant height also increased with the use of rootstocks. The increase in the dry weights of the leaves and fruits depended on rootstocks. They concluded that grafting improved plant growth and yield depending on the rootstock genotype. Grafting has the potential to be as a strategy to increase the tolerance of plants to promote water use efficiency (WUE). The present study was aimed to evaluate the grafting biochemical and physiological effects on inducing drought stress resistance in cucumber.
Materials and Methods: This experiment was conducted in complete randomized design with three replications and treatments are included grafted and ungrafted plants, and water potential level 0 (control), -0.4 and -0.8 MP. The Isfahan endemic cucumber specious as a scion with the hole method grafted on Ferro rootstock. The physiological and growth traits were measured. Photosynthesis (stomata conductance, photosynthesis, water use efficiency), growth (root and stem growth), and antioxidants (SOD, POD, protein) parameters, and transpiration were measured.
Results and Discussion: Result indicated that grafting with increasing root nutrient absorption and its development drought stress resistance improved. Although, grafting reduced potassium content. Grafting and the interaction of rootstock ×scion impressed many morphological and physiological characteristics. Under stress condition, some features improved plant water relationship, growth and development. Gas exchange indices like photosynthesis, transpiration and stomatal conductance were lower in grafted plant compare to ungrafted plants. Proline content was significantly increased in grafted treatments compare to ungrafted ones. Higher potassium under -0.8 MP in grafted plants showed the maintenance osmotic stability and potassium hemostasis were the draught stress mechanism in resistant rootstocks.
Conclusion: Finally, grafting as an efficient method to increase cucumber yield and improve drought resistance recommend. These results suggest that the use of drought tolerant Cucurbita rootstock can improve cucumber photosynthetic capacity under drought stress and consequently crop performance. The results revealed that grafted plants had better vegetative growth than  ungrafted (control) ones. Furthermore, photosynthetic parameter, antioxidant activity and fresh and dry weight of stem and leaves were improved, but grafting had no significant effect on fruit quality and yield. In conclusion it is recommended that grafting procedure in some crops include cucumber should be done only after assuring the benefits and risks of grafted seedlings.

Keywords

1- Albacete A., Martínez-Andújar C., Ghanem M.E., Acosta M., Sánchez-Bravo J., Asins M.J., Cuartero J., Lutts S., Dodd I.C., and Pérez-Alfocea F. 2009. Rootstock-mediated changes in xylem ionic and hormonal status are correlated with delayed leaf senescence, and increased leaf area and crop productivity in salinized tomato. Plant Cell and Environment 32: 928-938.
2- Ashraf M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances 27: 84-93.
3- Bates L., Waldren R.P., and Teare I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205-207.
4- Chance B., and Maehly A.C. 1955. Assay of catalases and peroxidase. MethodsinEnzymology 2: 764-775.
5- Chaumont F., and Tyerman S.D. 2014. Aquaporins highly regulated channels controlling plant water relations. Plant Physiology113: 233791.
6- Chaves M.M., Flexas J., and Pinheiro C. 2009. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany 103: 551-560.
7- Cuartero J., Boların M.C., Asıns M.J., and Moreno V. 2006. Increasing salt tolerance in the tomato. Journal of Experimental Botany 57: 1045-1058.
8- Ding L., Gao C., Li Y., Zhu Y., Xu G., Shen Q., Kaldenhoff R., Kai L., and Guo Sh. 2015. The enhanced drought tolerance of rice plants under ammonium is related to aquaporin (AQP). Plant Science234: 14-21.
9- Estan M.T., Martinez-Rodriguez M.M., Perez-Alfocea F., Flowers T.J., and Bolarin M.C. 2005. Grafting raises the salt tolerance of tomato through limiting the transport of sodium and chloride to the shoot. Journal of Experimental Botany 56: 703-712.
10- Ganjali A., Porsa H., and Bagheri A. 2011. Assessment of Iranian chickpea (Cicer arietinum L.) germplasms for drought tolerance. AgriculturalWater Management 98: 1477-1484.
11- García-Sánchez F., Syvertsen J., Gimeno V., Botía P., and Perez-Perez J.G. 2007. Responses to flooding and drought stress by two citrus rootstock seedlings with different water-use efficiency. Physiologia Plantarum130: 532-542.
12- Goreta S., Bucevic-Popovic V., Selak G.V., Pavela-Vrancic M., and Perica S. 2008. Vegetative growth, superoxide dismutase activity and ion concentration of salt-stressed watermelon as influenced by rootstock. The Journal of Agricultural Science 146: 695-704.
13- Huang Y., Tang R., Cao Q., and Bie Z. 2009a. Improving the fruit yield and quality of cucumber by grafting onto the salt tolerant rootstock under NaCl stress. Scientia Horticulturae 122: 26-31.
14- Huang Y., Zhu J., Zhen A., Chen L., and Bie Z.L. 2009b. Organic and inorganic solutes accumulation in the leaves and roots of grafted and ungrafted cucumber plants in response to NaCl stress. Journal of Food, Agriculture and Environment 7: 703-708.
15- Karaba A., Dixit S., Greco R., Aharoni A., Trijatmiko K.R., Marsch-Martinez N., Krishnan A., Nataraja K. N., Udayakumar M., and Pereira A. 2007. Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerant gene. Proceedings of the National Academy of Sciences of the United States of America 104: 15270-15275.
16- Martinez-Rodriguez M.M., Estan M.T., Moyano E., Garcia-Abellan J.O., Flores F.B., Campos J.F., Al-Azzawi M.J., Flowers T.J., and Bolarín M.C. 2008. The effectiveness grafting to improve salt tolerance in tomato when an ‘excluder’ genotype is used as scion. Environmental and Experimental Botany 63: 392-401.
17- Munns R., and Tester M. 2008. Mechanisms of salinity tolerance. Annual ReviewofPlant Biology 59: 651-681.
18- Rouphael Y., Cardarelli M., Colla G., and Rea E. 2008. Yield, mineral composition, water relations, and water use efficiency of grafted mini-watermelon plants under deficit irrigation. Horticultural Science 43: 730-736.
19- Sanders P.L., and Markhart A.H. 1992. Interspecific grafts demonstrate root system control of leaf water status in water-stressed Phaseolus. The Journalof Experimental Botany 43: 1563-1567.
20- Satisha J., Prakash G.S., Bhatt R.M., and Sampath Kumar P. 2007. Physiological mechanisms of water use efficiency in grape rootstocks under drought conditions. International Journal of Agricultural Research 2: 159-164.
21- Schwarz D., Rouphael Y., Colla G., and Venema J.H. 2010. Grafting as a tool to improve tolerance of vegetables to abiotic stresses: Thermal stress, water stress and organic pollutants. Scientia Horticulturae 127: 162-171.
22- Serraj R., and Sinclair T.R. 1996. Processes contributing to N2-fixation intensitivity to drought in the soybean cultivar Jackson. Crop Science 36: 961-968.
23- Sharp R.E., Poroyko V., Hejlek L.G., Spollen W.G., Springer G.K., Bohnert H.J., and Nguyen H.T. 2004. Root growth maintenance during water deficits: physiology tofunctional genomics. The Journal of Experimental Botany 55: 2343-2351.
24- Sing R.P., Chidambara Murthy K.N., and Jayaprakash G.K. 2002. Studies on the antioxidant activity of pomegranate peel and seed extracts using in vitro models. The Journal of Agricultural and Food Chemistry 50: 81-86.
25- Tanji K.K. 1990. Nature and extent of agricultural salinity.p. 537-582. In ED. k. k. TAnji.(ed). Agricultural Assessment and Management. American Society of Civil Engineers, New York.
26- Turner N.C. 2003. Adaptation to drought: lessons from studies with chickpea. Indian Journal of Plant Physiology 11-17. (Special Issue)
27- Uhrits R. 1974. Effect of the osmotic Pressure on water absorption germination of alfalfa seeds. American Journal of Botany 33: 278-285.
28- Yu L., Haley S., Perret J., Harris M., Wison J., and Qian M. 2002. Free radicalscavenging properties of wheat extracts. Agricultural and Food Chemistry 50: 1619-1624.
29- Zhong Y.Q., and Bie Z.L. 2007. Effects of grafting on the growth and quality of cucumber fruits. Acta Horticulturae 761: 341-347.
30- Zhu J., Bie Z.L., Huang Y., and Han X.X. 2008. Effect of grafting on the growth and ion concentrations of cucumber seedlings under NaCl stress. Soil ScienceandPlant Nutrition 54: 895-902.
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