بررسی تأثیرجدایه‌های باکتری Pseudomonas putida بر عملکرد قارچ خوراکی دکمه‌ای سفید (Agaricus bisporus)

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 جهاد دانشگاهی خراسان رضوی

چکیده

القای تشکیل ساختار ته‌سنجاقی در قارچ Agaricus bisporus به طور خاص، با کاهش دما و غلظت دی‌اکسیدکربن در حضور باکتری‌های موجود در خاک پوششی از جمله باکتری Pseudomonas putidaانجام می‌شود. این باکتری می‌تواند به عنوان محرک رشد بر روی عملکرد قارچ دکمه‌ای نیز تأثیر بگذارد. در این پژوهش 81 جدایه باکتری از نمونه‌های خاک پوششی 6 مزرعه پرورش قارچ خوراکی در سال 1394 جداسازی شد و در نهایت 33 جدایه که به عنوان باکتری P. putida شناسایی شدند، به خاک پوششی قارچ دکمه‌ای تلقیح گردیدند. نتایج آزمون‌های مزرعه‌ای نشان داد که جدایه‌های مورد بررسی نسبت به تیمار شاهد (بدون اعمال باکتری) تأثیر معنی‌داری بر روی وزن تر و تعداد قارچ دارند (p≤0.05)؛ به طوری که بیشترین وزن تر قارچ مربوط به تیمار جدایه‌های P27 وP13 به ترتیب به میزان 63/361، 8/342 گرم (تیمار شاهد 39/146 گرم) و بیشترین تعداد قارچ مربوط به تیمار جدایه‌های P18 و P24 به ترتیب با 21 و 83/20 عدد (تیمار شاهد 50/8 عدد) بر کیلوگرم کمپوست بود. در مرحله بعد توان تولید سیدروفور، توان تولید هورمون IAA، فعالیت آنزیم ACC دآمیناز و توانایی انحلال فسفات نامحلول در جدایه‌ها ارزیابی و رابطه هرکدام از آن‌ها با تعداد و وزن تر قارچ مورد بررسی قرار گرفت. نتایج نشان داد که بین میزان تولید هورمون IAA و وزن تر قارچ (58/0=r) و همچنین بین تولید هورمون IAA و تعداد قارچ (50/0=r) همبستگی معنی‌دار و مثبتی وجود دارد. پس می‌توان نتیجه گرفت که تولید IAA توسط باکتری ممکن است عامل تأثیرگذاری بر روی عملکرد قارچ دکمه‌ای ‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Influence of Pseudomonas putida Isolateson the Yield of Edible White Button Mushroom Agaricus bisporus

نویسندگان [English]

  • Mojtaba Lotfi 1
  • Mohammad Farsi 1
  • Amin Mirshamsi Kakhki 1
  • Javad Janpoor 2
1 Ferdowsi university of Mashhad
2 Culture and Research (ACECR), Mashhad branch, Iran
چکیده [English]

Introduction: Because of their high protein and mineral contents and low fat, calories and cholesterol, edible mushrooms such as Agaricus bisporus are an important part of the people diet in many countries, but in Iran, the yield of this mushroom is less than the average of yield in the world. Phase change from the vegetative to the reproductive stage and fruit body initiation of this mushroom depends on special physical, chemical and microbial properties of casing layer. Phase change is initiated by decreasing oftemperature and CO2 concentration and presence of some bacteria (such as Pseudomonas putida) in the casing layer. It is believed that P. putida may cause this process and increase the yield of A. bisporus by siderophore and hormone-like compounds secretion, decreasing the level of ethylene via ACC deaminase activity and dissolution of insoluble phosphate. The objective of this work was to identify P. putida isolates as growthpromoting bacteria isolated from A.bisporus casing soil and to evaluate their effect on mushroom yield.
Materials and Methods: In this study, 81 individual bacterial isolates were collected by screening the casing layer of 6 edible mushroom farms. Luria Bertani (LB) medium supplemented with sodium lauroyl sarcosine (SLS) and trimethoprim were used for isolation of Pseudomonas bacteria by plating serial dilutions of each soil sample. Finally, using species-specific primers, 33 isolates that identified as P. putida were selected and were used toinoculate
A. bisporuscasing layer. Inoculations were performed in a completely-randomized design with two replicates. The harvesting began when buttons were fully-grown (but not yet open), and the number of mushrooms and fresh (wet) weight of them were recorded after harvesting of each flush. In the next experiment IAA and siderophore production ability, ACC deaminase production capacity and ability of dissolving of insoluble phosphate in isolates and the correlation between these factors and number and fresh weight of mushroom were evaluated. Analysis of the data was carried out using JMP 8. Means were compared using Tukey’s test at p≤0.05.
Results and Discussion: The results of this study showed that the best stage for collecting P. putida is pinning, because the maximum number of identifiedP. putidawas recorded at this stage.Field experiment showed that different isolates have a significant effect on fresh weight and the number of mushrooms per kg compost compared to control (p≤0.05), so that the highest fresh weight observed in treatment of P27 and P13 isolates with 361.63 and 342.8 gr/kg compost respectively and the highest number of mushroom observed in treatment of P18 and P24 isolates with 21 and 20.83 mushroom per kg of compost,respectively. Interestingly, in this study, some isolates showed negative or no effect on mushroom yield which could be due to the interaction between bacteria and A. bisporus strain and/or complex conditions of casing layer. Other results showed that there is a positive and significant correlation between IAA production ability in P. putidaand fresh weight (r=0.58) and the number of mushrooms (r=0.50) in A. bisporus.Whereas there was no significant correlation between other factors and fresh weight and the number of mushrooms. IAA through promotion of cell elongation and differentiation increased mushroom growth and protein.This hormone is one of the needs of A.bisporus mushroom and it is very effective in growth and caused an increase in mushroom yield compared to other growth promoting factors.
Conclusions: In the present study, with the aim of investigation of the effect of P. putida on the yield of
A. bisporus and determining the most effective factor in this process, collectedisolatesinoculated to A. bisporuscasing layer and growth promoting factors in these isolates were evaluated. Results showed that the best stage for collecting P. putida is pinning. These bacteria havesignificant effects on fresh weight and the number of mushrooms.There is not significant correlation between other factors and fresh weight and the number of mushrooms. Based on the results, it could be said that the use of growth promoting bacteria in edible mushroom culturing could be resulted anincrease in mushroom yield and could be beneficial in production of healthy food. Finally, it could be said that P. putida isolates P27 and P13 may have the potential to act as a potential bio-fertilizer.

کلیدواژه‌ها [English]

  • Casing soil
  • Growth promoting bacteria
  • Indole-3-acetic acid
  • yield

مراجع

1- Abbas-Zadeh P., Saleh-Rastin N., Asadi-Rahmani H., Khavazi K., Soltani A., Shoary-Nejati A.R., and Miransari M. 2010. Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiologiae Plantarum, 32(2): 281-288.
2- Akhgar A.R., Arzanlou M., Bakker P.A.H.M., and Hamidpour M. 2014. Characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing Pseudomonas spp. in the rhizosphere of salt-stressed canola. Pedosphere, 24(4): 461-468.
3- Alam N., Amin S.M.R., and Sarker N.C. 2007. Efficacy of five different growth regulators on the yield and yield contributing attributes of Pleurotus ostreatus (Jacquin ex Fr.) Kummer. Bangladesh Journal of Mushroom, 1(1): 51-55.
4- Ali S.S., and Vidhale N.N. 2013. Bacterial siderophore and their application: a review. International Journal of Current Microbiology and Applied Science, 2(12): 303-312.
5- Alikhani H., Saleh Rastin N., and Bihamta M.R. 2007. An evaluation of auxin hormons and ACC deaminase production ability by Iranian soil rhizobial strains and the effect of superior strains applications on plant growth characteristics. Iranian Journal of Agricultural sciences (Journal of Agriculture), 38(4): 693-703. (In Parsian)
6- Bahrimi Teimoori B., Pourianfar H.R., Moeini M.J., and Janpoor J. 2014. Chemically and physically induced mutagenesis in basidiospores of oyster mushroom Pleurotus ostreatus var. florida. International Journal of Advanced Research, 2: 915-921.
7- Bradford M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-258.
8- Burg S.P. 1973. Ethylene in plant growth. Proceedings of the National Academy of Sciences, 70(2): 591-597.
9- Chen S., Qiu C., Huang T., Zhou W., Qi Y., Gao Y., Shen J., and Qiu L. 2013. Effect of 1-aminocyclopropane-1-carboxylic acid deaminase producing bacteria on the hyphal growth and primordium initiation of Agaricus bisporus. Fungal Ecology, 6(1): 110-118.
10- Colauto N.B., Fermor T.R., Eira A.F., and Linde G.A. 2016. Pseudomonas putida Stimulates Primordia on Agaricus bitorquis. Current Microbiology, 72(4): 482-488.
11- De Freitas j.R., Banerjee M.R., and Germida J.J. 1997. Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.). Biology and Fertility of Soils, 24(4): 358-364.
12- Ebadi A., Alikhani H.A., and Rashtbari M. 2012. Effect of plant growth promoting bacteria (PGPR) on the morpho-physiological properties of button mushroom Agaricus bisporus in two different culturing beds. International Research Journal of Basic and Applied Sciences, 3: 203-212.
13- Ebrihimi Karim Abad R., Rasouli Sadaghyani M., and Barrin M. 2015. Phosphate solubilizing microorganisms as biofertilizer. Agriculture and Natural Resources Engineering Organization of Islamic Republic of Iran Quarterly. 13(47): 22-25. (In Persian)
14- Farsi M., Jalalzadeh B., and Malekzadeh Kh. 2009. The breeding and production of hybrid straians of white button mushroom. Proceeding of the 6th Iranian Horticultural Science Congress, 2-15 Jul. 2009. Rasht, I. R. Iran. (in Parsian)
15- Farsi M., and Pourian Far H. 2011. Cultivation and breeding of white button mushroom. Jahad Daneshgahi Publications, Mashhad. (in Parsian)
16- Garcia-Fraile P., Menendez E., and Rivas R. 2015. Role of bacterial biofertilizers in agriculture and forestry. AIMS Bioengineering, 2(3): 183–205.
17- Ghods-Alavi B.S., Soleymani M., Ahmadzadeh M., and Soleymani S. 2013. Ability of rhizobacteria of valerian in phosphate solubilization and their symbiotic efficiency. Journal of Science and Technology of Greenhouse Culture, 4(13): 61-72. (in Parsian with English abstract)
18- Gitay A.S., And Wange S.S. 2008. Effect of diazotroph and phosphate solubilizing bacteria on mycelial growth of button mushroom. Annals of Plant Physiology, 2: 282-283.
19- Glick B.R., Penrose D.M., and Li J. 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of Theoretical Biology, 190(1): 63-68.
20- Glickmann E., and Dessaux Y. 1995. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and Environmental Microbiology, 61(2): 793-796.
21- Goltapeh E.M., Danesh Y.R., Kamal S., and Varma, A. 2009. Biology and molecular approaches in genetic improvement of cultivated button mushroom (Agaricus bisporus). In Symbiotic Fungi: 403-421. Springer Berlin Heidelberg.
22- Gordan H.R., Mahmoudnia Meymand M., Zou Alali J., Khatamirad M., and Farsi M. 2008. A study of potential of AFLP markers to genetic fingerprinting of the button mushroom, Agaricus bisporus. Journal of Plant Protection (Agricultural Science and Technology), 22(1): 27-36. (In Parsian with English abstract)
23- Gravel V., Antoun H., and Tweddell R.J. 2007. Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Tricoderma atroviride: possible role of indole acetic acid (IAA). Soil Biology and Biochemistry, 39:1968–1977.
24- Gülser C., and Pekşen A. 2003. Using tea waste as a new casing material in mushroom (Agaricus bisporus (L.) Sing.) cultivation. Bioresource Technology, 88(2): 153-156.
25- Guo X., Zou X., and Sun M. 2009. Effects of phytohormones on mycelial growth and exopolysaccharide biosynthesis of medicinal mushroom Phellinus linteus. Bioprocess and Biosystems Engineering, 32(5):701-707.
26- Hanning I., Jarquin R., Oleary A., and Slavik M. 2009. Polymerase Chain Reaction-based assays for the detection and differentiation of poultry significant Pseudomonads. Journal of Rapid Methods & Automation in Microbiology, 17(4): 490-502.
27- Henry M.B., Lynch J.M., and Fermor T.R. 1991. Role of siderophores in the biocontrol of Pseudomonas tolaasii by fluorescent pseudomonad antagonists. Journal of Applied Bacteriology, 70(2): 104-108.
28- Janpoor J., and Farsi M. 2013. Assessment of total RNA extraction from edible mushroom (Agaricus bisporus) With Three Current Methods. International Journal of Farming and Allied Sciences. 2(18): 662-664.
29- Jia Y.J., Ito H., Matsui H., and Honma M. 2000. 1-aminocyclopropane-1-carboxylate (ACC) deaminase induced by ACC synthesized and accumulated in Penicillium citrinum intracellular spaces. Bioscience, biotechnology and biochemistry, 64(2): 299-305.
30- Kaneko M., and Tanimoto E. 2009. Auxin-regulation of hyphal elongation and spore germination in arbuscular mycorrhizal fungus, Gigaspora margarita. In International Symposium “Root Research and Applications” RootRAP, 2-4 Sep. 2009. International Society of Root Research., Boku–Vienna, Austria.
31- Kavousi H.R., Farsi M., and Shahriari F. 2008. Comparison of random amplified polymorphic DNA markers and morphological characters in identification of homokaryon isolates of white button mushroom (Agaricus bisporus). Pakistan Journal of Biological Sciences, 11(14): 1771-1778.
32- Khaki Pour N., Khavazi K., and Akhgar A. 2012. Identification of indolic compounds synthesized by fluorescent Pseudomonads and their effect on Rapeseed growth. Iranian Journal of Soil Research (soil and water science). 26 (4): 416-423. (in Parsian)
33- Khalili H.R., Olfati J.A., and Fallahi A. 2013. Plant growth promoting rhizobacteria affect button mushroom yield and quality. Horticulture, Biology and Environment, 4(2): 83-89.
34- Khosravi H. 2013. Biofertilizers containing plant growth promoting rhizobacteria: strengths and weaknesses. Land Management Journal, 1(1): 33-46. (in Parsian).
35- Lifshitz R., Kloepper J.W., Kozlowski M., Simonson C., Carlson J., Tipping E.M., and Zaleska I. 1987. Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putida under gnotobiotic conditions. Canadian Journal of Microbiology, 33(5): 390-395.
36- Maghami M., Olamaee M., Rasuli Sadaghiani M.H., and Dordipour E. 2014. Isolation and identification of Psedomonas fluorescens and evaluation of their plant growth promoting properties in soils Golestan province. Journal of Soil Management and Sustainable Production, 3(2): 251-264. (In Parsian with English abstract)
37- Mayak S., Tivosh T., and Glick B.R. 1999. Effect of wild type and mutant plant growth-promoting rhizobacteria on the rooting of mungbeen cuttings. Journal of Plant Growth Regulation, 18:49–53.
38- Mohammad A.O., and Sabaa A.E. 2013. Impact of some Pseudomonas spp. isolated from casing soil on the hyphal growth of Agaricus bisporus. Canadian Journal on Computing in Mathematics, Natural Sciences, Engineering and Medicine, 4 (1): 45-48.
39- Motesharrei Z., Mahmoodi H., Owlia P., and Salimi H. 2014. Study of ACC deaminase activity of Rhizobacteria isolated from soils of northern Iran. Biological Journal of Microorganisms, 11: 37-46. (in Parsian with English abstract).
40- Nagata T., Oobo T., and Aozasa, O. 2013. Efficacy of a bacterial siderophore, pyoverdine, to supply iron to Solanum lycopersicum plants. Journal of bioscience and bioengineering, 115(6): 686-690.
41- Pastor N., Rosas S., Luna V., and Rovera, M. 2014. Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants. Symbiosis, 62(3):157-167.
42- Patel D., Jha C.K., Tank N., and Saraf M. 2012. Growth enhancement of chickpea in saline soils using plant growth-promoting rhizobacteria. Journal of Plant Growth Regulation, 31: 53–62.
43- Patten C. L., and Glick B. R. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and Environmental Microbiology, 68:3795–3801.
44- Pecchia J., Cortese R., Albert I., and Singh M. 2014. Investigation into the microbial community changes that occur in the casing layer during cropping of the white button mushroom, Agaricus bisporus. In Proceedings of 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8), 19-22 Nov. 2014. New Delhi, India.
45- Penrose D. M., and Glick B.R. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum, 118: 10-15.
46- Prathap M. 2015. A critical review on plant growth promoting rhizobacteria. Journal of Plant Pathology & Microbiology, 6(4).
47- Pratiksha K., Narute T.K., Surabhi S., Ganesh A., and Sujoy, S. 2017. Effect of liquid biofertilizers on the yield of button mushroom. Journal of Mycopathological Research, 55(2): 135-141.
48- Rasouli Sadaghiani H., Khavazi K., Rahimian H., Malakouti M. J., and Asadi Rahmani H. 2005. Population density and identification of fluorescens Pseudomonas associated with rhizosphere of Wheat. Journal of Soil and Water science, 26(3): 195-206. (in Parsian with English abstract)
49- Rezaeian S., Pourianfar H.R., and Janpoor J. 2016. Collection and identification of Iranian wild mushrooms: towards establishment of a mushroom bio-bank. International Journal of Advanced Research, 4(1):256-60.
50- Riahi H., Eskash A., and Shariatmadari Z. 2011, October. Effect of bacterial and cyanobacterial culture on growth, quality and yield of Agaricus bisporus. In Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, 4-7 Oct. 2011. Arcachon, France.
51- Roca A., Pizarro-Tobias P., Udaondo Z., Fernandez M., Matilla M.A., Molina-Henares M.A., Molina L., Segura A., Duque A., and Ramos J.L. 2013. Analysis of the plant growth-promoting properties encoded by the genome of the rhizobacterium Pseudomonas putida BIRD1. Environmental Microbiology, 15:780–794.
52- Roupas P., Keogh J., Noakes M., Margetts C., and Taylor P. 2012. The role of edible mushrooms in health: Evaluation of the evidence. Journal of Functional Foods, 4(4): 687-709.
53- Rupak M., Sandipan Ch., Bishnu P. Ch., and Arun K. G. 2005. Enhancement of biomass production of edible mushroom Pleurotus sajor-caju grown in whey by plant growth hormones. Process Biochemistry, 40: 1241–1244.
54- Saghafi D., Ali Alikhani H., and Motesharezadeh B. 2014. Effectiveness of some native Rhizobium spp. containing ACC-deaminase in amelioration of negative effects of stress ethylene on growth parameters in Oilseed rape (Brassica napus L.). Iranian Journal of Field Crop Science, 45(3): 367-376. (in Parsian)
55- Sayyed R.Z., Chincholkar S.B., Reddy M.S., Gangurde N.S., and Patel P.R., 2013. Siderophore producing PGPR for crop nutrition and phytopathogen suppression. p. 449-471. In D. K. Maheshwari (ed.) Bacteria in Agrobiology: Disease Management. Springer, Berlin, Heidelberg.
56- Shah S., Li J., Moffatt B.A., and Glick B. R. 1998. Isolation and characterization of ACC deaminase genes from two different plant growth-promoting rhizobacteria. Canadian Journal of Microbiology, 44(9): 833-843.
57- Sharma A., and Johri B.N. 2003. Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiological Research, 158(3):243-248.
58- Sharma, A., Johri, B.N., Sharma, A.K. and Glick, B.R., 2003. Plant growth-promoting bacterium Pseudomonas sp. strain GRP3 influences iron acquisition in mung bean (Vigna radiata L. Wilzeck). Soil Biology and Biochemistry, 35(7): 887-894.
59- Silva-stenico M.E., Pacheco F.T.H., Rodrigues J.L.M., Carrilho E., and Tsai S. M. 2005. Growth and siderophore production of Xylella fastidiosa under iron-limited conditions. Microbiological Research, 160: 429-436.
60- Singh M., Singh R.P., Chaube H.S., and van Griensven L.J.L.D. 2000. Siderophore producing bacteria as potential biocontrol agents of mushroom diseases. Mushroom Science, 15: 577-585.
61- Siyoum N.A., Surridge K., van der Linde E.J., and Korsten L. 2015. Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product. Annals of Microbiology, 66(1): 151-164.
62- Sperber J.I. 1958. The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research, 9: 778-781.
63- Tomar R. 1998. Effect of phosphate-solubilizing bacteria and farmyard manure on the yield of blackgram (Phaseolus mungo). The Indian Journal of Agricultural Sciences, 68(2): 81-83.
64- Turner E.M., Wright M., Ward T., Osborne D.J., and Self R. 1975. Production of ethylene and other volatiles and changes in cellulase and laccase activities during the life cycle of the cultivated mushroom, Agaricus bisporus. Microbiology, 91(1): 167-176.
65- Vansuyt G., Robin A., Briat J.F., Curie C., and Lemanceau P. 2007. Iron acquisition from Fe-pyoverdine by Arabidopsis thaliana. Molecular Plant-Microbe Interactions, 20(4): 441-447.
66- Yeo S. G., and Hayes W. A. 1981. Solubilisation and utilisation of phosphorus by Agaricus bisporus (Lange) Pilat. Mushroom Science, 11: 73-91.
67- Zarenejad, F., Yakhchali, B. and Rasooli, I. 2012. Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production. World Journal of Microbiology and Biotechnology, 28(1): 99-104.
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  • تاریخ دریافت: 23 بهمن 1395
  • تاریخ پذیرش: 23 بهمن 1395
  • تاریخ اولین انتشار: 01 شهریور 1397

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