بررسی اثر برخی مالچ‌های آلی بر افزایش مقاومت به سرما درگیاه تاج الملوک (Aquilegia sp.)

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه فردوسی مشهد

چکیده

برای بررسی اثر تنش یخ زدگی و ارزیابی تحمل به آن با استفاده از انواع مالچ‌‌های آلی در گیاه تاج الملوک تحت شرایط کنترل شده، این آزمایش به صورت فاکتوریل بر پایه طرح کاملاً تصادفی با چهار تکرار در دانشکده کشاورزی دانشگاه فردوسی مشهد اجرا گردید. تیمار‌های آزمایشی شامل 4 نوع مالچ در ترکیب با خاک ( شاهد (بدون استفاده از مالچ)، 50 درصد خاک+50 درصد کود دامی، 50 درصد خاک+ 50 درصد سوزنی برگ، 50 درصد خاک + 50 درصد سبوس برنج) و 5 سطح دمای یخ زدگی (0، 5-، 10-، 15-، 20- درجه سانتیگراد) بود. جهت ارزیابی تحمل به دماهای یخ زدگی درصد نشت الکترولیت‌ها، تعداد برگ، سطح سبز برگ، وزن خشک بوته و محتوای پرولین برگی اندازه گیری شد. نتایج نشان داد با کاهش دما از 0 به 20- درجه سانتیگراد قطر ساقه، سطح برگ و تعداد برگ در تیمار مالچ سبوس به ترتیب 6/42، 4/73، 2/21 درصد، در مالچ سوزنی برگ 2/35، 9/64، 6/47  درصد ، در مالچ کودی 1/ 20، 4/46، 8/7 درصد و در شاهد مالچ 8/32، 79، 7/30 درصد کاهش یافت در دمای 5- درجه سانتیگراد مقدار پرولین در برگ 26 درصد و در دمای 20- 50 درصد نسبت به شاهد افزایش داشت. همچنین کمترین مقدار پرولین (73/0 میکرومول بر گرم وزن تر) در گیاهان تحت تیمار با مالچ سبوس بود. به طور کلی، تنش یخبندان باعث کاهش صفات مورفولوژیک اندازه گیری شده در تاج الملوک شد ولی استفاده از مالچ­های آلی منجر به بهبود این صفات گردید به طوریکه بهترین نتایج در مالچ کود دامی بدست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of some Organic Mulches on Cold Resistance of Aquilegia sp.

نویسندگان [English]

  • Y. Selahvarzi
  • M. Kamali
  • J. Nabati
  • H. Ahmadpour Mir
Ferdowsi University of Mashhad
چکیده [English]

 
Introduction: Each year, with the onset of cold season and severe drop in temperature, the probability of frost bite and frost damage is a problem for landscaping plants. Many plant species, especially tropical and subtropical species, are damaged when exposed to frostbite, causing damage to their cells, tissues, and organs. Research has shown that by altering membrane properties during cold stress, metabolic balance is disturbed and with the increase in toxic metabolites, secondary damage to the plant can occur. At low temperature, decreases the efficiency of energy transfer to the center of the photosystem II. In addition, low temperatures are the main cause of the formation of reactive oxygen radicals. Also, lowering the temperature in the presence of light, due to the imbalance between light absorption and photosynthesis, increases the risk of light oxidation. Low temperature also reduces the activity of Rubisco. The amount of free proline in many plants increases significantly in response to environmental stresses such as frost stress, and stabilizes the membrane during cold stress.On the other hand, the use of some organic materials such as organic mulches increase temperature of the soil, and thus helps plant from frostbite. Use of organic mulch is widespread in agriculture due to the positive effect in soil temperature, weed control and moisture retention. Also, these mulches are effective in height, growth and flowering, early maturity and total yield of the products. Mulches in the warm seasons reduces soil temperature. Use of mulch can also help plants to withstand frostbite. Organic mulch decomposition in appropriate temperature and humidity conditions, liberates the nutrients gradually and provides for root plant and microorganisms of the soil. Organic mulches can reduce the effect of salt toxicity on plant growth and actively increase soil desalination. The most important benefit of mulch is the increase in soil temperature in the seed area, which accelerates the growth and yield of the product. Use of straw as mulch resulted in accelerated germination in cucumber. Use of straw mulch leads to an increase in temperature at night, thus protecting plants from temperature stress that has a positive effect on the growth and development of wheat.
Material and methods: In order to investigate the effect of freezing stress and using different types of organic mulch for Aquilegia plant, this experiment was conducted as a factorial experiment based on completely randomized design with four replications at Faculty of Agriculture, Ferdowsi University of Mashhad. The experimental treatments included four types of mulch (control (without mulch), 50% soil + 50% manure, 50% soil + 50% leaf needle + 50% soil + 50% rice bran) and five levels of freezing temperature (0, -5, -10, -15 and 20). Characteristics such as percentage of electrolyte leakage, relative water content, chlorophyll index and total chlorophyll, leaves number, leaf area, plant dry weight and proline leaf content were considered.
Results and Discussion: The results showed with decrease of temperature from 0 to -20 °C, stem diameter, leaf area and leaf number in bran mulch treatment decreased by 42.6%, 73.4%, 21.2% respectively, also stem diameter, leaf area and leaf number in mulch of leaf needle were 35.2%, 9/64%, 47.6%, in manure mulch were 20.20%, 46.4%, 7.8% and in the control of mulch decreased, 32.8%, 79.7%, 30.7%, respectively. At -5 °C, the amount of proline was 26% in the leaf and at -20°C, the amount of proline increased 50% compared to the control. Also, the lowest proline (0.73 μmol / g fresh weight) was obtained from the plants that treated with bran mulch. With application of, electrolyte leakage reached 63.6%, 68%, 61% and 57% in control conditions bran, needle and manure, respectively. In short, the least electrolyte leakage was observed in manure. On the other hand, when temperature dropped from 0 to -20 °C, the percentage of electrolyte leakage increased in Aquilegia. Relative water content of the leaf were 24% at 0°C, 38% at -15 °C and 23% at -20 °C. In terms of non-use of mulch, the relative water content was 36% and reached a 42% and 40% with application of manure and needle using mulch. By measuring the total carbohydrate found in Aquilegia leaf, it was observed that the amount of this trait was increased under frost stress. In general, although frost stress reduced the morphological traits of Aquilegia, use of organic mulch resulted in the improvement of these traits. The best results were observed in manure mulch.

کلیدواژه‌ها [English]

  • Dry weight
  • Carbohydrate
  • Electrolyte leakage
  • Proline
  • total chlorophyll
1. Adeniyi O. T., Akparobi S. O., and Ekanayake, J. 2004. Field studies on chlorophyll a fluorescence for low temperature tolerance testing of cassava (Manihot esculenta Crantz). Food, Agriculture and Environment, 2(1): 166-170.
2. Azizi, H., Nezami, A., Nasiri Mahalati, M., Khazaee, H.R., 2007. Evaluation of freezing tolerance of wheat cultivars under controlled conditions. Journal of Iranian Field Crops Research, 6(1), 109-119. [In Persian with English Summary].
3. Badaruddin M., Reynolds M. P., and Ageeb O. A. A. 1999. Wheat management in warm environments: Effect of organic and inorganic fertilizers, irrigation frequency and mulching. Agronomy Journal, 91, 975-983.
4. Bagheri A., Nezami A., and Soltani, M. 2000. Breeding cold-blooded beans to tolerate stress (translation). Agricultural Research, Training and Promotion Organization. P 446.
5. Bates. L. S., Waldran. R. P. and Teare, I. D. 1973. Rapid determination of free proline for water studies. Plant Soil, 39:205-208.
6. Bizhani Sh., Salehi H., Jokar A., and Daneshmand B. 2013. Cold tolerance and antioxidant response of Chaman Poa and Papalom. Journal of Production and Processing of Agricultural and Horticultural Products, 5(17): 229-237.
7. Bonachela S; (Granados, M.R, Lopez, J.C, Hernandez, J, Magan, J.J, Baeza, E.J and Baille, A). 2012. How plastic mulches affect the thermal and radiative microclimate in an unheated low-cost greenhouse. Agricultural and Forest Meteorology, 152, 65-72.
8. Bowker, M. (Edinger, Philip).1989. "Lawn and Grand Covers".Sunset publishing.
9. Cardona C.A., Duncan R.R., and Lindstorm O. 1997. Low temperature tolerance assessment in Paspalum. Crop Science, 37:1283-1291.
10. Cardona C.A., Duncan R.R., and Lindstrom O. 1997. Low temperature tolerance assessment in Paspalum. Crop Science. 37: 1283-1291.
11. Dashti M., Kafi M., Tavakoli H., Mirza M., and Nezami A. 2013. Effects of freezing stress on Morpho-physiological indices and chlorophyll fluorescence of Salvia leriifolia Benth. Seedlings.Journal of Plant Researches. 28(5): 962-973.
12. Dere S., Gunes T., and Sivaci R. 1998. Spectrophotometric determination of chlorophyll a, b and total carotenoid contents of some algae species using different solvents. American Journal of Botany, 22: 13-17.
13. Dubois D., Gilleres K.A., and Hamilton J.K. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 350-356.
14. Dunn J. H., Bughrara S. S., Warmund M. R., and Fresenburg B. F. 1999. Low temperature tolerance of zoysia grasses. Horticulture Science 34: 96-99.
15. Duryea Marry L; (English, R. Jeffery and Hermansen, L. Annie). 1999. "A Comparison of Landscape Mulches: Chemical, Allelopathic, And Decomposition Properties". Journal of Arboriculture, 2, 88-97.
16. Eugenia M., Nunes S., and Ray Smith G. 2003. Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Science 43: 1349-1357.
17. Farahmand H., Sarcheshme pour M., Safari R., and Nazari F., 2011. Reduction of evaporation using mulch in green space. Eleventh General Irrigation Seminar and Evaporation Reduction.
18. Fry J. D., Lang N. S. and Clifton R. G. P. 1991. Freezing resistance and carbohydrate composition of 'Floratam' St. Augustinegrass. Horticulture Science, 26: 1537-1539.
19. Ghasemi Ghahsare M., and Kafi M. 2015. Scientific and practical flowering
20. Ghosh P.K; (Dayal, Devi, Bandyopadhyay, K.K and Mohanty M). 2006. "Evaluation of straw and polythene mulch for enhancing productivity of irrigated summer groundnut". Field Crops Research, 99, 76–86.
21. Griesbach R.J., and Berberich S.M. 1995. The early history of research on ornamental plants at the U S. Department of Agriculture from 1862 to 1940. Horticultural Science 30: 421–425.
22. Griffith M., and Yaish M. W. F. 2004. Antifreeze proteins in overwintering plants: a tale of two activities. Trends in Plant Science, 9: 399-405.
23. Hajo Hosseini A., Javad Pour Y., and Razavi M. 2011. Frostbite in agriculture and ways to control it. Technical and Promotional Journal of Yazd Province Agricultural Organization. 3-19.
24. Izadi darbandi E., Yosef sani M., Nezami A., Musavi M.J., Keikha F., and Nezami S. 2012. Effect of freezing stress on Dianthus barbatus. Environmental Stresses in Crop Sciences, 4(2):117-125.
25. Jodaugienė D., Pupalienė R., Sinkevičienė A., Marcinkevičienė A., Žebrauskaitė M. Baltaduonytė K., and Čepulienė R. 2010. The influence of organic mulches on soil biological properties. Zemdirbyste-Agriculture, 97 (2): 33-40
26. Lies Jeffery.K; (Dosmann, Michael.S). 1999. "Effect of organic and mineral mulches on soil properties and growth of Fairview Flame® red maple trees". Journal of Arboriculture, 3, 163-167.
27. Malek M.M., Galeshi S., Zeinali A., Ajam N., and Malek M. 2012. Investigation of leaf area index, dry matter and crop growth rate on the yield and yield components of soybean cultivars. Electronic Journal of Crop Production, 5(4):1-17.
28. Mir Mohammadi A.M. 2005. Physiological and breeding aspects of cold and frost tensions in crops. Gulben Publishing House of Isfahan.
29. Munns R. 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment, 25: 239–250.
30. Naidu B. and Thusitha G. 2005. Increasing cold tolerance in rice by selecting for high polyamine and gibberellic acid content. Australian Journal of Plant Physiology 25: 793-800.
31. Nezami A., Borzooei A., Jahani M., Azizi M., and Sharif A. 2007. Electrolyte leakage as an indicator of freezing injury in colza (Brassica napus L.). Iranian Journal of Field Crops Research. 5: 167-175
32. Ramakrishna A.; (Tam, Hoang Minh, Wani, Suhas.P and Lomg, Tranh Dinh). 2006. Effect of mulch on soil temperature, moisture, weed infestation and yield of groundnut in northern Vietnam. Field Crops Research, 95, 115–125.
33. Rashed Mohassel M.H., Nezami A., Bagheri A., Haj Mohammadnia K., Bannayan M. 2009. Evaluation of freezing tolerance of two fennel ecotypes under controlled conditions. Journal of Herbs, Spices and Medicinal Plants, 15(1): 131-140.
34. Rashed Mohassel M.H., Nezami A., Bagheri A., Haj Mohammadnia K., Bannayan M. 2009. Evaluation of freezing tolerance of two fennel ecotypes under controlled conditions. Journal of Herbs Spices Medicinal Plants, 15, 131-140.
35. Roe N. E., Stofella J., and Ggreatz D. 1997. Compost from various municipal solid wastes feed stocks affect vegetable crops, II Growth, yield and fruit quality. J. ASHS. 122: 433-437.
36. Shaikh A. El., and Fouda T. 2008. Effect of different mulching types on soil temperature and cucumber production under Libyan conditions.
37. Shooshtarian S., A., Salehi H., and Tehrani far A. 2011. Study of the growth and development characteristics of ten plant species in the Kish Island green area during the warm season. Journal of Agroecology, 4:514-524.
38. Smartt J. 1994. The groundnut crops. A scientific basis for improvement. London. Chapman and Hall. pp: 734-735
39. Steward Larry G; (Davis Sydnor, T and Bishop, Bert). 2003. "The ease of ignition of 13 landscape mulches". Journal of Arboriculture, 6, 317-321.
40. Teutonica R. A., Palta J.P., and Osborn T. C. 1993. In vitro freezing tolerance in relation to winter survival of rapeseed cultivars. Crop Science, 33: 103-107.
41. Warmund R.M., Guinan P., and Fernandez G. 2008. Temperatures and cold damage tosmall fruit crops across the eastern United States associated with the April 2007 freeze. Horticultural Science 43: 1643-1647.
42. Zhang G.S. (Hu, X.B, Zhang, X.X and Li, J.C). 2013. Effect of plastic mulch and winter catch crop on water availability andvegetable yield in a rain-fed vegetable cropping system at mid-Yunnan plateau, China. Scientia Horticulturae, 164, 333-339. 5(4):1-17.
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