Morteza Alizadeh; Bahman Hosseini
Abstract
Hyssop (Hyssopus officinalis L.), belongs to the family of Lamiaceae which its origin is Asia Minor, and is considered as one of the most important medicinal plant. This experiment was done to study the effects of population and BAP concentrations on hyssop plant regeneration under in vitro culture conditions. ...
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Hyssop (Hyssopus officinalis L.), belongs to the family of Lamiaceae which its origin is Asia Minor, and is considered as one of the most important medicinal plant. This experiment was done to study the effects of population and BAP concentrations on hyssop plant regeneration under in vitro culture conditions. This research were performed in factorial experiment with three populations (Hamadan, Shiraz and Mashhad) and four levels of BAP (0, 2.2, 4.4, 13.2 µM), in three replications. Results showed that there is significant difference between populations. In all three populations, the lowest average and percentage of regeneration was observed in MS medium without any growth regulators. In Hamadan population, the highest average of regenerated shoots (7.86) and percentage of regeneration (86%) was obtained in BAP (4/4 µM) and in Shiraz population, maximum number of regenerated shoots (7) and 80% regeneration percentage was observed on 4.4 µM BAP. The minimum regeneration percentage with 0% was obtained in MS medium without plant growth regulators and MS media supplemented with 13.2 µM concentration of BAP. 2.2 µM BAP in Mashhad population with an average of 9 regenerated shoots and 86% regeneration percentage was known as the best treatment. ANOVA results showed significant (P < 0.01) effect of IBA levels on root induction. The highest rooting percentage (86.66%) was achieved in MS medium supplemented with 9.84 µM of IBA and the lowest rooting percentage (0%) was observed in 1/2 MS hormone-free medium.
Bahman Hosseini; Haleh Hashemi; Farajollah Shahriari; Seyyed Hassan Marashi
Abstract
Abstract
Benzylisoquinoline alkaloids are a diverse group of nitrogenous compounds which is found in about 20% of plant species. Isolation of effective genes involved in morphine biosynthesis of opium poppy is very important in the production of specific which can be achieved using metabolic engineering ...
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Abstract
Benzylisoquinoline alkaloids are a diverse group of nitrogenous compounds which is found in about 20% of plant species. Isolation of effective genes involved in morphine biosynthesis of opium poppy is very important in the production of specific which can be achieved using metabolic engineering techniques. In this biosynthesis pathway, the key enzyme SAT is Involved in the conversion of salutaridinol 7-O-acetyltransferase (EC 2.3.1.150) to salutaridinol-7-O-acetate, which is the immediate precursor of thebaine. In this project, the gene encoding this enzyme was isolated using primers which were designed on the basis of the gene sequence available on data banks (NCBI) for papaver somniferum. This gene IS then cloned in expression vectors under the Control of Camv 355 promoter. The result of this cloning was confirmed using different molecular methods such as enzyme digestion and PCR. Agro infiltration method was also used for transient expression of SAT gene. The result of evaluation showed that morphine and codeine were only Produced in the leaves of transgenic plants containing SAT transgen.
Keywords: Benzylisoquinoline alkaloids, Salutaridinol 7-O-acetyltransferase, opium poppy
