M. Eisapoor; Kh. Hemmati; N. Hemmati
Abstract
Introduction: Horsemint (Mentha longifolia L.) is a valuable medicinal and aromatic plant belonging to Lamiaceae family. It was reported that the contents and composition of perceived pharmacological properties varied significantly among populations. The genus Mentha L. (Lamiaceae), is widely distributed ...
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Introduction: Horsemint (Mentha longifolia L.) is a valuable medicinal and aromatic plant belonging to Lamiaceae family. It was reported that the contents and composition of perceived pharmacological properties varied significantly among populations. The genus Mentha L. (Lamiaceae), is widely distributed in all continents (except in South America and Antarctica). The systematic of the genus is not very elucidated because of the strong morphologic variations, levels of ploidy (2n = 2x = 24 to 2n = 6x = 96), and hybridizations intra- and interspecific (or between spontaneous and cultivated forms). M. longifolia or horsemint is a fast-growing and perennial herb which cultivated in all regions. The species possesses antimicrobial and antioxidant properties. The antimicrobial properties of the essential oils can be attributed to the presence of pulegone, menthone and neomenthone because they can destroy the structure of different polysaccharides, fatty acids and phospholipids by altering the permeability of the cell membrane and destroying the bacterial wall. The essential oils of horsemint are said to be cyclohexane and aromatic, and pulegone is the main compound of these oils has a definite aroma in the range of intense to spicy mint. The effects of environmental conditions on the plant growth and development, reproduction and distribution are well known in plant ecology. Many studies have been carried out about the relationships among plant chemical contents, biological activity and environmental variables in the natural and cultivated plant species. This information has been used to determine the medicinal value and economic importance of plant products.
Materials and Methods: In this research, the effects of six habitats with different altitudes (habitats of Amol city) on some morphological and biochemical characteristics of horsemint plants were investigated in a completely randomized design with three replications. This study was done to compare the amount of pulegone, total phenol, total flavonoids, antioxidant activity, chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and also morphological features of horsemint such as number of leaves per plant, number of branches per plant, number of flowers and number of nodes. For antioxidant properties using DPPH method in 517 nm wavelength, total amount of phenol using Folin-Ciocalteu method in 765 nm wavelength and the total amount of flavonoid were done using the aluminum chloride method in 415 nm wavelength and they were measured by spectrophotometer. Essential oil was extracted from dried aerial parts using distillation by Clevenger apparatus for 3 hours. Gas chromatography (GC) was used to measure pulegone.
Results and Discussion: The results showed that the altitude and ecotype significantly affecting the growth and biochemical characteristics of horsemint plant. The highest number of leaves (25) and the shoots (27) of horsemint were obtained in the Gat Lash region with 2000 meters and Siozmin with 1850 meters above sea level, respectively. Gat Lash and Siozmin had the highest and lowest content of photosynthetic pigments, respectively. Although there was no significant difference between the content of phenol and flavonoids in different habitats, the highest rates were observed in the Pardemeh and Gat Lash habitats. The results showed that the highest and lowest oil were obtained from ecotypes of Gat Lesh with 2000 meters and Vash Verin with 2300 meters above sea level, respectively. As the height increased, the amount of pulegone was also increased in the leaves, so that the highest amount of pulegone was found in Pardemeh )6069 ppm) habitat by 2600 meters above sea level.
Conclusions: Generally, for obtaining the highest essential oil content and pulegone, the best ecotypes were Gat Lash and Pardemeh habitats, respectively. Due to the importance and usage of secondary metabolites in the human life, investigating the relationship between environmental conditions with the production and accumulation of secondary metabolites in plants can be very useful. Our results showed that the climatic conditions had a significant effect on the morphological characteristics of the horsemint, thereby increasing or decreasing the essential oil. In the present study, the best habitat for the highest pulegone was Pardemeh habitat but the habitat of Gat Lash could be a suitable habitat for domestication and cultivation of this plant because of its higher essential oil yield and relatively good pulegone content. For this purpose, it is recommended to study different habitats of this species in other parts of the country for finding the best ecotypes.
Kh. Hemmati; B. Ardavanpour; M. Ghazaeian; V. Akbarpour
Abstract
Introduction: Essential oils make up a large part of aromatic substances in plants. Generally, essential oils are considered as remnants of main processes of plants metabolism, especially in stressful conditions. Summer savory (Satureja hortensis L.) is one of the most important plants in Lamiaceae family, ...
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Introduction: Essential oils make up a large part of aromatic substances in plants. Generally, essential oils are considered as remnants of main processes of plants metabolism, especially in stressful conditions. Summer savory (Satureja hortensis L.) is one of the most important plants in Lamiaceae family, which it’s more than 30 species grown in the East Mediterranean. It is one of the oldest plants that have been used as vegetables and medicinal and aromatic plants. Dried summer savory has been introduced as one of the most pleasant spices that are planting in large areas of farmland in many countries. Generally, the aerial parts of it, that is usually harvested at flowering stage, has therapeutic effects such as facilitating digestion, stomach tonic, diuretic, astringent, carminative, anti-diarrhea and anti-worm. Summer savory essential oil is used in food (conserves and beverages) and pharmaceutical industries. Various studies on S. hortensis essential oil has been showed that it contains high amounts of phenolic compounds such as Carvacrol, γ-Terpinene, Thymol, p-Cymene, β-Caryophyllene, Linalool and other terpenoids.
Organic fertilizers have been known to improve the biodiversity and may prove a large depository for excess carbon dioxide. These fertilizers increase the number of soil organisms by providing organic matter and micronutrients for organisms such as fungal mycorrhizae, (which aid plants in absorbing nutrients), and can drastically reduce external inputs of pesticides, energy and fertilizer, at the cost of decreased yield. Nevertheless they are as effective as chemical fertilizers over longer periods of use.
The aim of this project was to study the effects of different organic matters on vegetative and reproductive growth and percentage and components of essential oil in three Satureja ecotypes in Gorgan, Golestan, Iran climate.
Materials and Methods: This study was designed as a factorial experiment based on Randomized Complete Block design with four replications and done in research farm of Agriculture and Natural Resource, University of Gorgan in 2010. Treatments were cattle manure, municipal solid waste, leaves compost, the mixture of them and control. Three ecotypes of summer savory studied in this experiment were Kordestan, Shiraz and Yazd.
The plants were harvested at full flowering stage, when they have the highest essential oil and were dried in oven at 45 °C. The essential oils of dried samples were isolated by hydro distillation for 3 h, using a Clevenger-type apparatus.
Statistical analysis was performed by SAS software and the mean comparison were measures by using Duncan tests at 5% level of probability.
Results and Discussion: The analysis of variance showed that media had significant effect on yield components in summer savory. There were no significant differences between ecotypes and media*ecotype effect on some parameters of yield components. The cattle manure showed positive effects on bush height in this experiment. Hendawy et al (2010) showed that the application of cattle manure and sheep manure had reached significantly the 5% level of plant height and essential oils percentages. They concluded that sheep manure have macro and micro nutrients which provide thyme plants with their requirements from these elements. Essential oil percentage also, tended to increase with application of organic fertilizers either cattle manure, sheep manure or compost. Khalil (2002) showed the effect of two kind of organic fertilizers on Rosmarinus officinalis and Jasminum spp growth parameters and yield components. In this experiment organic manure treatments showed increasing in plant fresh and dry weight. This could be related to positive effect of organic manure on soil texture and fertility. Ahmadian et al. (2006) showed that the animal manure significantly enhanced the number of umbers per plant, the number of seed per plant, the biological and seed yield in Cuminum cyminum. Animal manure significantly enhanced cumin aldehyde and r-cymene and decreased b-pinene, g-terpinene and a-pinene in cumin oil.
Due to the results, treatments had no significant effects on essential oil percentages, but were effective on essential oil components. Hussein et al. (2006) showed that compost levels combined with different distances had a pronounced effect on the various essential oil constituents in Dracocephalum moldavica. In this experiment, Although Shiraz ecotype showed the maximum essential oil percentage (1.2 percent) but there was no significant differences between ecotypes. Among essential oil components, the maximum percentages of Carvacrol achieved in mixture media and Yazd ecotype. Control treatment showed the highest amount of γ- terpinen and paracymene in compare to other media. Shiraz and Kordestan showed also, the highest percentages of γ- terpinen and paracymene, respectively. Plants in mixture media had maximum levels of Carvacrol (74.69 percent). Between ecotypes, Yazd had maximum Carvacrol percentages. Between media, Control treatment was maximum contents of γ –terpinen (5.48) and paracymene (1.76) and other treatments had reduction effects on essential oil components. Between ecotypes, the highest γ-terpinen percentage (5/79) was in Shiraz ecotype and the highest p-cymene percentage (1/69) was in kordistan ecotype.
Conclusion: Therefore, by considering the fertilization of savory plants with different organic manure fertilizers in the form of compost that are economic in each region is recommended.
hossein sabourifard; Azim qaesmnezhad; Khodayar hemmati; Aboutaleb hezarjaribi; Mahmoudreza bahrami; Fahimeh nosrati
Abstract
Introduction: One of the most important requirements in planning production and processing of medicinal plants in order to obtain high yield and high-quality is the initial assessment of the physical and chemical properties of soil, which reduces the production cost by avoiding the use of unnecessary ...
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Introduction: One of the most important requirements in planning production and processing of medicinal plants in order to obtain high yield and high-quality is the initial assessment of the physical and chemical properties of soil, which reduces the production cost by avoiding the use of unnecessary soil analysis. Summer savory (Satureja hortensis L.) is one the most widely used medicinal plants that quality index of plant is related to the quantity and the constituent of its essential oil content. Understanding the relations between the quantity and quality of medicinal plants with the very physical and chemical properties of soil is very complex and the estimation of parameters changes of medicinal plants affect by soil quality characteristics is more difficult. Today, with the arrival of multivariable regression models and artificial lattice models in the research, many complex relationships found in nature is understandable. Hence the need for estimation the biomass yield of savory using fast, cheap and with acceptable accuracy is feeling.
Materials and Methods: The present study was performed at the Agricultural Research Station Neyshabur as pot experiment based on a completely randomized design with three replications. Around 53 soil samples were collected from different parts of Neyshabur city, and soil texture, organic matter, pH, salinity, phosphorus, potassium, nitrogen and carbon content were selected as the easily available parameters. Before planting the parameters were measured in laboratory. Approximately 90 days after planting seeds in pots containing soil samples, the sampling of plants was done based on the treatments. For drying, samples were placed for 24 hours in an oven at 40 °C. Finally, the relationship between the biomass yield and easily available soil parameters was determined using artificial neural network by Matlab7.9 software.
Results and Discussion: The results showed that soil variability, is a key element in the management of valuable information on soil properties within a field and valuable information on soil properties within a field nature puts at our disposal. In yield modeling with 10 parameters for 53 soil samples, the best makeup hidden layer with Levenberg-Marquardt algorithm training as a hidden layer, 58 neurons, logsig threshold function for hidden layer and Tansig for the output layer were selected. High values of R2 and low levels of RMSE mentioned the proximity of the forecast data with measurement data and high accuracy of the model in summer savory biomass yield estimation. To obtain the most sensitive parameters, the sensitivity analysis was calculated using no-sensitive coefficient. So that, if the coefficient of a sensitive parameter is more than 1.0, the mentioned parameter, is one of the critical parameters of model. Accordingly, the parameters of organic carbon, nitrogen, phosphorus, organic matter, potassium, pH, salinity, clay, silt and sand respectively were selected as the most sensitive parameters. The addition of input parameters increases the value of R2 and reduces the RMSE during training, validation and test stages. This represents an increasing in the accuracy of model in estimation of biomass yield via increasing the input parameters. Models 1(soil texture) and 2(carbon) are not enough strong for biomass yield estimation. With increasing the experiment from 1 to 2, the potency of the neural network model 3(soil texture + carbon) significantly increased. Thus with an overview, the model No. 3 suggested as an improved model because with the minimum number of imputes produced equal output comparing the models with more inputs.
Conclusions: Based on the obtained results, it seems that with the improvement of artificial neural network models and determining appropriate parameters, results to understanding the soil factors involved in the formation of savory plant biomass and better planning. Till leads to a cheaper and better product. Also, results showed that the artificial neural network has high accuracy in estimating the biomass plant Summer Savory. So that, the 80% of yield variability of the study area, presents by using the data of 10 readily available properties of the soil. Yield biomass of savory, largely depends on the soil texture, organic matter, carbon and the minerals of the soil. Since, this study is the first work to estimate the biomass of medicinal plants using artificial neural network, therefore recommended to use this method to estimate the yield and essential oil of other medicinal plants.
Farnaz Moghadami; khodayar hemmati; Vahid Akbarpour
Abstract
Introduction: Citrus is in the first rank in the world with respect to production among fruits. They are grown commercially in more than 50 countries around the world. Several factors such as rootstock, nutrition and irrigation regimes, cultivation design and etc. may affect the promotion of quality ...
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Introduction: Citrus is in the first rank in the world with respect to production among fruits. They are grown commercially in more than 50 countries around the world. Several factors such as rootstock, nutrition and irrigation regimes, cultivation design and etc. may affect the promotion of quality and quantity of fruits. Lemon fruits are among the most valuable functional diets shown to lower oxidative-related disease risks, particularly cardiovascular disease. A well-documented characteristic of these fruits is the accumulation of high amounts of glycoside flavonones, named hesperidin in the fruits. Several recent studies have demonstrated that the cytoprotective action of citrus fruits is enhanced by the presence of antioxidants including vitamin C, phenolics, carotenoids and flavonoids. Hesperidin and its metabolites significantly have been found to lower the total cholesterol and triglyceride concentrations in plasma. Hesperidin acts as a chemopreventive agent against colon carcinogenesis induced by azoxymethane. Lemons are one of the citrus species having many medicinal effects owing to their secondary metabolites. Flavonoids are a part of aromatic polyphenols having different biological actions such as antioxidant activity, anti-cancer, anti-sensation and etc. Hesperidin is one of the abundant secondary metabolites belonging to flavonoids in citrus, which is effective in treating many diseases.
Materials and Methods: In order to investigate the effect of lemon fruits (Citrus lemon cv. Cluster, C. lemon cv. Lisbon and C. lemon cv. Eureka) tissue type and harvesting time on the flavonoids hesperidin content, this experiment was carried out. Thus, this research was conducted in a factorial arrangement on the basis of completely randomized design with three factors: tissue type (Flavedo, Albedo and Flesh), harvesting time (45, 75, 105 and 135 days after full bloom) and three lemon cultivars (Cluster, Lisbon and Eureka) with three replications. Studied trees in Kotra Research Station were planted for research aims and were 20-year grafted on sour orange rootstocks. For selection of trees, the same height, crown, and other morphological attributes were considered. As the fruit quality may be differ in different parts of crown, fruits were harvested from different geographical orientations of crown. Samples were gathered after full bloom at the 30-day intervals. The measured parameters were included fruit weight, diameter, peel thickness, dry matter and hesperidin flavonoid in extraction of various fruit tissues. Extraction was conducted by methanol and hesperidin content recorded by High Performance Liquid Choromatography (HPLC). Chemicals and reagents used in this study were high quality grade and acquired from Sigma-Aldrich
Results and Discussion: Results showed that the fruit harvesting time significantly affected on fruit diameter, peel thickness, dry matter and also hesperidin content of tissue extraction. Fruit diameter variation was related to the fruit weight. The highest fruit weight attained in cultivars Lisbon and Eureka (88.39 g and 84.72 g, respectively) at the fourth harvesting time (135 days after full bloom) and the minimum, in sour lemon cv. Cluster (4.16 g) at the first harvesting time (45 days after full bloom). The maximum of fruit diameter was recorded in cultivar Lisbon (54.81 mm) at the fourth harvesting time and the minimum diameter in cultivar Eureka (20.75 mm) at the first harvesting time. Fruit peel thickness at the first harvesting stage increased and then decreased. Cluster sour lemon cultivar possessed the highest peel thickness (7.16 mm) and the Eureka cultivar had the minimum (4.66 mm). Percentage of dry matter increased at the first harvesting stage and then decreased. The maximum percentage of dry matter was recorded in cluster sour lemon cultivar (22.83%) and minimum in Lisbon lemon cultivar (11.36%). The highest amount of hesperidin was observed in Lisbon cultivar (1606.93 ppm). These amounts decreased afterward until fruit harvesting time. The highest hesperidin content was produced in albedo tissue. Lisbon lemon in the hesperidin was better than the other two cultivars. It is concluded that the most suitable time of mature fruits harvesting is 45 days after full bloom. At this time the fruit diameter of lemon cv. Eureka, Lisbon and Cluster were 20.7, 22.8 and 25.8 mm; respectively. Hesperidin flavonoid was produced in albedo tissue in all cultivars. Therefor; fruit albedo tissue can be regarded as the best tissue for extracting hesperidin. Hesperidin was severely affected by harvesting time, tissue and cultivar. Hesperidin was achieved in the most amounts at the first harvesting stage in Lisbon lemon compared to all studied cultivars. As the fresh consumption of Eureka, Lisbon and specially Cluster lemon is few, thus preparing programs to extract flavonoid and use this cultivar in food industries is suggested.
A. Asefie Najaf Abadi; khodayar hemmati; azim ghasemnejad; mina ghazaeian; Pouneh Ebrahimi
Abstract
Abstract
The harvesting time and storage conditions of olive fruit are effective factors on the quality and quantity of oil. The harvesting time of olive is different in each area and it depends on climatic conditions.This investigation was carried out to assess the impact of harvesting time on olive ...
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Abstract
The harvesting time and storage conditions of olive fruit are effective factors on the quality and quantity of oil. The harvesting time of olive is different in each area and it depends on climatic conditions.This investigation was carried out to assess the impact of harvesting time on olive oil quality and quantity frome two olive varieties cultivated in Gorgan region. For this reason fruits of selected cultivars, Mishen and kroniki, were harvested with fifteen-day intervals from 6 september till middle desember. Then quality and quantity of oil was determined. The results showed that dry matter, oil content (in dry), free fatty acids increased during ripening, whereas peroxide value decreased during ripening. Therefore, the best harvesting time for these cultivars is beginning of December.
Keywords: Olive oil, Harvesting time, Oil quality and Quantity
