Sheno Amini; Mostafa Arab; Majid Rahemi; Abdolrahman Rahimi
Abstract
This research was carried out in order to evaluate the influence of different concentrations of ethanol and methanol (0, 4, 6 and 12%) and two pulse treatments (12 and 24 hour) on longevity of cut standard-carnation (Sensi cultivar) in a factorial based on randomized completely design with three replications, ...
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This research was carried out in order to evaluate the influence of different concentrations of ethanol and methanol (0, 4, 6 and 12%) and two pulse treatments (12 and 24 hour) on longevity of cut standard-carnation (Sensi cultivar) in a factorial based on randomized completely design with three replications, at Tehran University, Aboreyhan Pardis, horticulture department, during 2010. The results showed that the effect of alcohol treatments and interaction effect of alcohols and pulse on vase life were significant (at what level?????), whereas solution uptake and relative fresh weight were not affected by mentioned treatments. The highest (17.33 day) and lowest (11 day) amount of carnation vase life were obtained by ethanol 12% at pulse of 12 hours and control treatments, respectively. Ethanol 6% at pulse of 24 hours, ethanol 12% and methanol 6% at pulse of 12 hours were more effective in increasing the longevity of carnation than other pulse, while in other treatments, no significant differences were observed between the pulse times. Moreover, the highest of ethylene production obtained by control, while ethanol 4% and 6% at pulse of 24 hours, ethanol and methanol 12% at pulse of 12 hours and methanol 6% at both time of pulse markedly decreased the production of ethylene and lead to significantly increase in vase life. The relative extension of flowers and relative fresh weight had a positive correlation with vase life, while solution uptake had non correlation or negative correlation with other treats.
Seyyedeh Mahdiyeh Kharrazi; Seyyed Hossein Nemati; Ali Tehranifar; Abdolreza Bagheri; Ahmad Sharifi
Abstract
Carnation is considered as the world's third most important cut flower. Tissue culture techniques offer suitable method for micropropagation of this ornamental plant. However, one of the problems during in vitro culture of carnation is vitrification. Ratio of ammonium to nitrate and agar concentrations ...
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Carnation is considered as the world's third most important cut flower. Tissue culture techniques offer suitable method for micropropagation of this ornamental plant. However, one of the problems during in vitro culture of carnation is vitrification. Ratio of ammonium to nitrate and agar concentrations in the medium affect this phenomenon. Therefore, in this study the effect of these factors on the rate of proliferation and the vitrification of carnation (Dianthus caryophyllus L.) cultivar Innove Orange Bogr, was evaluated. In this investigation lateral buds were cultured on MS medium containing 1 mg/l BA, 0.1 mg/l NAA and with different concentrations of agar and different ratio of ammonium to nitrate. The results showed that increasing in the agar concentration to 12 g/l lead to decreasing the rate of vitrification but regeneration also declined. Increase of agar concentration cause limitation in nutrient absorption by plants. Also, decrease in the ratio of ammonium to nitrate in the medium reduces the amount of vitrification, but did not result in adverse effects on plant regeneration rates. Multiple regressions showed that the effect of ammonium to nitrate ratio on vitrification was higher than agar concentration. So by considering the amount of shoot regeneration and vitrification, to obtain the most normal shoots, the concentration of 10 g/l agar with ammonium to nitrate ratio 1:6, is recommended.
