Farid Moradinezhad; Sara Hassan Pour; Mohammad Hassan Sayyari Zahan
Abstract
Introduction: Seedless barberry (Berberis vulgaris L. var. asperma) is one of the most important horticultural and medicinal crops and also valuable fruit in South Khorasan province. Fresh barberry fruit has a very short shelf life, due to the sensitive juicy fruit tissue in the ripening stage and decay ...
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Introduction: Seedless barberry (Berberis vulgaris L. var. asperma) is one of the most important horticultural and medicinal crops and also valuable fruit in South Khorasan province. Fresh barberry fruit has a very short shelf life, due to the sensitive juicy fruit tissue in the ripening stage and decay during storage. Hence, most of the harvested fruits are dried traditionally or processed as juice, jam, and other products. However, the quality of both fresh and dried fruit is low due to different environmental stresses such as drought and salt stress and consequently deficiency of minerals during growth and development and also at harvest and during storage. It has been reported that pre-harvest foliar spray of calcium salts and salicylic acid can improve resistance to pathogens before harvest and reduce physiological disorders during handling and storage in many fruits. However, there is no information regarding the effect of calcium and salicylic acid spray as well as storage duration on fresh seedless barberry fruit. The objective of the current study, therefore, was to investigate the effects of the preharvest application of calcium chloride and salicylic acid on physiological attributes and postharvest quality of barberry fruits during long-term cold storage.
Materials and Methods: The experiment was conducted as split-plot in time and in a randomized complete block design with five treatments and in three replicates in the Research Orchard of Agricultural Faculty of the University of Birjand during 2014. Fifteen similar and 25-years old trees were used for the experiment. Pre-harvest treatments of foliar spraying were salicylic acid (1 and 3 mM) and calcium chloride (1 and 2%) and distilled water as control. Sprays were applied at 30 and 15 days before harvest. Fruits were harvested at commercial ripening stage, packed in plastic boxes and stored at cold room at 4±0.5 °C and 85±5 RH. Physicochemical and quality evaluations including fruit fresh weight and volume, and juice volumes of 100 berries, pH, total soluble solids (TSS), titrable acidity (TA), ascorbic acid, weight loss, electrolyte leakage of fruit and calcium content of leave and fruit were measured at harvest and after 30, 60 and 90 days of storage. Analysis of data was performed using Gen Stat program (ver 12) and comparison of means was done by LSD test at 5% probability.
Results and Discussion: The analysis of variance showed that the main effects of foliar spray with calcium chloride, salicylic acid and interaction of treatments were significant in storage time. The results showed that 1 mM of salicylic acid increased the fruit fresh weight, fruit volume and fruit juice, pH, total soluble solids, ascorbic acid and titrable acidity compared to other treatments. It has been reported that salicylic acid increases cell division and growth resulting in greater yield. It was also observed that application of salicylic acid at 3mM reduced electrolyte leakage significantly that can be useful to reduce chilling injury. The lowest weight loss was obtained in sprayed fruit with calcium chloride (2%) during cold storage, in agreement with the previouswork on plum. Foliar application of calcium chloride increased the calcium content of leave and fruit, although calcium application was not a significant effect in fruit tissue. Generally, using of chemical treatments increased the physicochemical characteristics of the fruit in seedless barberry, among them calcium chloride 2% and salicylic acid at 1 mM were most effective.
Conclusions: It can be concluded that pre-harvest spraying of seedless barberry shrub with salicylic acid and calcium chloride can improve the quality of fruit as well as extend the storage life. This is because a foliar application with salicylic acid and calcium chloride significantly affected fresh fruit weight, weight loss, fruit volume, electrolyte leakage, pH, TSS, TA and ascorbic acid in treated fruit compared to the control. Salicylic acid at 3 mM significantly decreased electrolyte leakage, which can be used as a useful tool for increasing resistance to chilling injury during long-term storage. However, further studies are needed as the effect of chemical sprays on fruit quality and quantity properties depend on different factors such as tree age and types, environmental conditions during and after the spray and also chemical concentration.
Mohammadreza Asghari; mohammad Tohidian
Abstract
Introduction: Asaclimactericfruit, peach has a highrespiration rateandvery lowshelf life. Nowadays theuse ofappropriatepostharvesttechnologiesto increase fruit postharvest lifeis necessary.Use of nanotechnologyis considered asan effective method to increase fruit postharvest life. Nanotechnology isused ...
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Introduction: Asaclimactericfruit, peach has a highrespiration rateandvery lowshelf life. Nowadays theuse ofappropriatepostharvesttechnologiesto increase fruit postharvest lifeis necessary.Use of nanotechnologyis considered asan effective method to increase fruit postharvest life. Nanotechnology isused extensivelyinallstages of production, processing, storage, packagingand transport ofagriculturalproducts.The objective of this study was to investigate the effect of silver and silica nanocomposites, nanosilver and polypropylene containers on shelf-life and preservation of peach qualitative characteristics (Prunuspersicacv. Elberta) such as titrable acidity, soluble solids, ascorbic acid, total antioxidant and total phenolics content in cold storage after the harvest.
Materials and Methods: To determine the effect of nanopackaging onshelf life andqualitativecharacteristics ofElberta peaches,the experiment was conducted in a factorial based on a completely randomized design with two treatmentsand four replications.The first factor consisted of nanopackagingnanosilver, silver and silica nanocompositesandpolypropylenepackaging, and the second factor was coldstorage periods of15, 30 and 45 daysattemperaturesfrom 0 to0/5 °C and a relative humidityof 85to 95%.In general, the experiment consisted of 36 experimental units, and each includinga container with four Elberta peach fruits.Data were analyzed by using SAS software, and Microsoft Office Excel 2010 software was used to plot the graphs.Duncan's multiple range test was used to compare the means of treatments.
Results and Discussion: At the end of the maintenance period, the highest firmness of peach texture was related to nanosilver containers. The main cause of fruit softening is the destruction of cell wall components, especially pectin, which is caused by certain enzymes such as polygalactronase. Whenstorage timeincreased, polypropylene containers showed a higher weight loss compared to nanocomposite containers, thus the results indicated that nanopackaging had a greater effect on preventing weight loss, which could be attributed to the formation of better barrier against water molecules. Nanoparticles and polypropylene containers up to 30-daystorage period preserved the pH of the product to some extent and prevented it from increasing too much. However, with an increase in storage time from 30 to 45 days, the pH of all dishes showed sharpincrease. In most treatments, fruits grew during the maintenance period, which is due to the reduction of organic acids. The lowest levels of organic acids reduction were recorded in polypropylene and nanosilver treatments. Generally, organic acids decreased as a result of respiration or becoming sugars, whichcan bedirectly related to metabolic activity. Maintaining organic acids in fruits is the result of a slowdown in the processes of ripeningand aging and reduction inrespiration and other metabolic activities. The results showed that in all the three treatments, the amount of soluble solids up to 15-day periodshowed an increasing trend, and after the end of day 30, it stopped in nanosilver and polypropylene treatments, and soluble solids were retained but reduced in nanocomposite treatment showed that this decrease. Nanocomposite containers exhibited the best performance in maintaining ascorbic acid content and preventing its changes, while ascorbic acid content in nanosilver and polypropylene containers varied greatly, thoughthere were no significant differences among thetreatments. Low oxygen environments, such as packaging containers, can inhibit ascorbic acid changes during maintenance by inhibiting oxidation. The results showed that total antioxidant level was almost identical in all treatments at the end of maintenance. Antioxidant activity has a close relationship with phenolic compounds, flavonoids and vitamins. The antioxidant capacity of fruits and vegetables is related to enzymatic and non-enzymatic compounds such as vitamin C, phenolic compounds, and carotenoids. Treatments reducingrespiration and producingethylene can reduce the rate of aging,the rate of free radical production and the consumption of antioxidants. Based on the results, on the day 30 in all treatments, the amount of phenolic compounds initiallyincreased, which is normal in red fruits, but afterwards decreased in all treatments mainly due to the increased activity of the polyphenol oxidase enzyme and the progression of aging. Increasing the amount of phenol on the day 30 can be attributed to decreased activity of the polyphenol oxidase enzyme and the oxidative stress associated with high concentrations of oxygen.
Conclusion: Nanopackaging increased the shelf life and qualitative properties of peach fruit. Nanotechnology has a simple and fast process for industrialization compared to other methods of keeping food products. As a result, the use of modern technologies such as nanopackaging can improve the taste, favor, quality of food and the storage of crops, especially fruits and vegetables.
Gholam Hossein Davarynejad; S. Arefkhani; Majid azizi; M. Zarei
Abstract
In order to evaluate the effect of different concentrations of postharvest salicylic acid and calcium chloride on shelf life, quality characteristics and antioxidant activity of peach fruit cv. Amesden, an experiment was conducted as factorial based on randomized completely design with three replications. ...
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In order to evaluate the effect of different concentrations of postharvest salicylic acid and calcium chloride on shelf life, quality characteristics and antioxidant activity of peach fruit cv. Amesden, an experiment was conducted as factorial based on randomized completely design with three replications. Fruits were harvested at the commercial ripening stage, and fruits were immerged in different concentrations of salicylic acid (1 and 2 mM), calcium chloride (1.5 and 3%), combined salicylic acid and calcium chloride (1-1.5, 1-3, 2-1.5 and 2-3), and distilled water (control) for 5 min, then fruits were packed in boxes with polyethylene cover and stored at 4°C with 80-85% relative humidity for 35 days. The changes in weight loss, fruit firmness, rot percentage; pH, total soluble solids, titratable acidity, ascorbic acid and antioxidant activity were estimated in 0 and 35 days during storage. The results showed that the weight loss, rot percentage, pH and total soluble solids significantly increased, while the fruit firmness, titratable acidity, ascorbic acid and antioxidant activity significantly decreased at the end of storage period. The salicylic acid and calcium chloride treatments significantly reduced the weight loss and maintained their firmness. In this condition, the highest of titratable acidity, ascorbic acid and antioxidant activity were observed in treatments of salicylic acid and calcium chloride, while the lowest of total soluble solids and rot percentage was showed in treatments of salicylic acid and calcium chloride than in the control treatment. Also, combined treatment (salicylic acid + calcium chloride) had an important effect in relative to each treatment separately. The data indicated that the use of salicylic acid and calcium chloride may be introduced as an effective and successful strategy in postharvest technology of the peach.
Z. Bearanvand; Naser Alemzadeh Ansari; M. Mosavi; A. Golpaigani
Abstract
In order to study, non-enzymatic antioxidants level of carotenoid and ascorbic acid in parsley leaves of twenty-one masses were compared between the first and second harvest. Also for evaluation of enzymatic-antioxidant activity, catalase and peroxidase activity in leaves of two twenty-one masses were ...
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In order to study, non-enzymatic antioxidants level of carotenoid and ascorbic acid in parsley leaves of twenty-one masses were compared between the first and second harvest. Also for evaluation of enzymatic-antioxidant activity, catalase and peroxidase activity in leaves of two twenty-one masses were measured at the end of vegetative growth. In each replication, one gram of fresh mixed leaf tissue of all samples was selected to measure the above mentioned parameters based on standard methods. , The data were analyzed in a randomized complete block design by SAS software. Results showed that the highest and lowest levels of carotenoids in the first harvest were7.54 and 2.00 mg/100 g in Bushehr 149 and Lorestan 69 masses, respectively. The maximum and minimum levels of vitamin C in the first harvest were 0.733 and 0.039 mg/100g in Lorestan 153 and western Azarbaijan 51 masses, respectively. The highest and lowest peroxidase activity were found 3.25 and 0.11 micromole of hydrogen peroxide per minute in central mass 46 and control region (native Ahwazi) respectively. The highest and lowest catalase activity were related 0.645 and 0.006 micromole of hydrogen peroxide per minute to Eastern Azerbaijan 62 and Central 45. According to results of this study, Lorestan 153, Lorestan 69, and Hamadan 49 masses could be introduced as the best masses of antioxidant activity.
F. Abri; M GH; R.H. Sajedi; Davoud Bakhshi; M.A. Shiri
Abstract
In this study, effect of different concentration of ascorbic acid (AsA, 0, 2, 4 and 6 mM) on delaying senescence in cut rose flowers cv. Royall Class was investigated based on factorial design. The results showed that the greatest delay in senescence was obtained with 4 mM AsA as compared to control ...
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In this study, effect of different concentration of ascorbic acid (AsA, 0, 2, 4 and 6 mM) on delaying senescence in cut rose flowers cv. Royall Class was investigated based on factorial design. The results showed that the greatest delay in senescence was obtained with 4 mM AsA as compared to control (distilled water). Therefore, fresh weight and diameter of flowers treated with 4 mM AsA was more than others treatments. Biochemical attributes such as protein, proline, anthocyanin content and lipid per oxidation levels were determined only in DW and 4 mM AsA. Application of 4 mM AsA treatments was shown to retard degradation of protein over 8 days vase life. Proline content in AsA treated flowers was slightly lower than DW flowers but no significant differences were found in treated flowers with AsA. Anthocyanin content declined gradually with onset of senescence, while AsA treatments suppressed decreasing its content. Therefore, treatment with 4 mM AsA suppressed malondialdehyde accumulation and delayed flowers senescence.
E. Ardakani; Gholam Hossein Davarynejad; Majid azizi
Abstract
In order to investigation the effect of salicylic acid and temperature treatments onphysic-chemical attributes and antioxidant activity was conducted of apricot cultivar ‘Lasgerdi’the experiment.The apricot fruits were harvested at the commercial ripening stage, and fruits were immerged in different ...
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In order to investigation the effect of salicylic acid and temperature treatments onphysic-chemical attributes and antioxidant activity was conducted of apricot cultivar ‘Lasgerdi’the experiment.The apricot fruits were harvested at the commercial ripening stage, and fruits were immerged in different concentration of salicylic acid as well as distilled water (control) for 5 min, then fruits were packed in boxes with polyethylene cover and stored. The changes in fruit weight, firmness, pH, total soluble solid content, titratable acidity, ascorbic acid, antioxidant activity were estimated after 0, 5, 10, 15 and 20 days during storage. The results showed that the weight loss, firmness, titratable acidity, ascorbic acid and antioxidant activity decreased significantly while the pH and total soluble solid content increased significantly during storage for two temperatures. During the storage period, a significant difference between treatments (control and various concentrations differences of salicylic acid) and various temperature significantly the weight loss and maintained their firmness. In this condition, the highest and lowest of titratable acidity, ascorbic acid and antioxidant activity were observed in treatments of 4 mM salicylic acid and control, respectively. Also, the data showed that the temperature of 4°c positive impact maintained quality and fruit shelf life during storage. Based on our results, the salicylic acid treatment as combination nature and temperature of 4°c may be used commercially to extend the storage life of apricot.
E. Ardakani; Gholam Hossein Davarynejad; Majid azizi
Abstract
The limited postharvest storage life of apricot causes the goal of presenting a solution in this manuscript to improve the postharvest storage of studied apricot cultivars. Studying the effect of different concentration of pre-harvest salicylic acid on quality attributes and antioxidant activity of apricot ...
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The limited postharvest storage life of apricot causes the goal of presenting a solution in this manuscript to improve the postharvest storage of studied apricot cultivars. Studying the effect of different concentration of pre-harvest salicylic acid on quality attributes and antioxidant activity of apricot during storage is the approach in used. The apricot cultivar(Nori) were sprayed at the 3 weeks before commercial ripening stage, with 1, 2, 3 and 4 mM salicylic acid as well as distilled water (control). At commercial ripening stage, apricot fruit were harvested and immediately transported to the laboratory, and then fruits were packed in boxes with polyethylene cover and stored at 4°C and 95% relative humidity for 20 days. The changes in weight loss, fruit firmness, total soluble solids, titratable acidity, pH, ascorbic acid, total phenolics and antioxidant activity were estimated after 0, 5, 10, 15 and 20 days during storage. The results showed that the weight loss, total soluble solids, pH increased significantly while the fruit firmness, titratable acidity, ascorbic acid, total phenolics and antioxidant activity decreased significantly during storage for both cultivars. During this stage, a significant difference between control and salicylic acid treatments in all measured parameters is observed. The salicylic acid treatments reduced significantly the weight loss and maintained their firmness. In this condition, the highest and lowest of titratable acidity, ascorbic acid, total phenolics and antioxidant activity were observed in treatments of 4 mM salicylic acid and control, respectively. The data revealed that the quality of apricot fruits was improved by the use of salicylic acid treatment due to its effect on delaying the ripening processes.
