ارزیابی برخی از ویژگی‌های فیزیولوژیکی و بیوشیمیایی میوه در بعضی از ارقام انگور (Vitis vinifera L.)

نوع مقاله : مقالات پژوهشی

نویسندگان

• عبدالله گودرزی ¹
• مهراب یادگاری ²
• سیداصغر موسوی ^{1، 3}
• حبیب الله نوربخش ^{1، 4}

¹ گروه زراعت و گیاهان دارویی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

² مرکز تحقیقات تغذیه و محصولات ارگانیک، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

³ بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهارمحال و بختیاری، سازمان تحقیقات، آموزش و ترویج کشاورزی، شهرکرد، ایران

⁴ بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان چهار محال و بختیاری، سازمان تحقیقات، آموزش و ترویج کشاورزی، شهرکرد، ایران

10.22067/jhs.2025.89578.1374

چکیده

این پژوهش با هدف مطالعه صفات ریخت‌شناختی، عملکردی و غلظت برخی عناصر غذایی در 12 رقم تجاری انگور (Vitis vinifera L.) ̓̓بی‌دانه قرمز̒، ̓̓فلیم سیدلس̒، ̓̓پرلت̒، ̓̓کاشمری 1̒، ̓̓شاهرودی̒، ̓̓یاقوتی̒، ̓̓کاشمری 2̒، ̓̓عسگری دایی نصرالله̒، ̓̓ریش بابا̒، ̓̓سیاه محلی̒، ̓عسگری̒ و ̓̓سیاه حسن آباد̒) طی دو فصل رشد 1401-1400 و 1402-1401 در باغ کلکسیون ارقام انگور در ایستگاه تحقیقات باغبانی منطقه سامان در استان چهارمحال و بختیاری در قالب طرح بلوک­های کامل تصادفی انجام شد. نتایج بیانگر تفاوت معنی­دار ارقام مورد مطالعه از نظر خصوصیات رشدی (قطر تنه، طول شاخه یک‌ساله، تعداد و فاصله میان‌گره و سطح برگ) می­باشد. همچنین صفات مربوط به عملکرد بوته شامل طول خوشه، عرض خوشه، وزن خوشه، طول حبه و عرض حبه به­طور معنی­داری تحت تأثیر نوع رقم انگور بود و بیشترین عملکرد در رقم ̓̓فلیم سیدلس̒ (19/7 کیلوگرم در بوته تاک) ثبت گردید. براساس نتایج حاصل، ارقام مورد ارزیابی انگور از نظر غلظت عناصر غذایی پرمصرف و کم‌مصرف در برگ تفاوت معنی­داری با یکدیگر داشتند. نتایج همبستگی بیانگر اثر مثبت و معنی­دار سطح برگ، مشخصات خوشه، و غلظت عناصر غذایی نیتروژن، فسفر، پتاسیم و منیزیم، آهن، منگنز، روی و بور بر عملکرد بوته تاک انگور می­باشد. نتایج تجزیه خوشه­ای، ارقام مورد مطالعه انگور را در سه گروه عملکرد بالا، متوسط و پایین طبقه­بندی کرد. نتیجه تجزیه به مؤلفه­های اصلی نیز مطابق با نتایج حاصل از تجزیه خوشه­ای بود. براساس یافته­های این پژوهش، ارقام ̓̓پرلت̒، ̓فلیم سیدلس̒، ̓̓بی‌دانه قرمز̒، ̓سیاه حسن آباد̒ و ̓ریش بابا̒ از نظر صفات مورد ارزیابی نسبت به سایر ارقام برتر بوده که می­توان برای مطالعات و برنامه­های به­نژادی بیشتر مورد بررسی قرار داد.

کلیدواژه‌ها

• ارقام تجاری انگور
• تجزیه خوشه‌ای
• تجزیه همبستگی
• عملکرد بوته
• عناصر غذایی پرمصرف

موضوعات

• میوه کاری

عنوان مقاله [English]

Evaluation of Physiological and Biochemical Properties in some Grape (Vitis vinifera L.) Cultivars

نویسندگان [English]

• A. Goodarzi ¹
• M. Yadegari ²
• S.A. Mousavi ^{1 3}
• S.H. Nourbakhsh ^{1 4}

¹ Department of Agriculture and Medicinal Plants, Shk.C., Islamic Azad University, Shahrekord, Iran

² Research Center of Nutrition and Organic Products, Shk.C., Islamic Azad University, Shahrekord, Iran

³ Horticulture Crops Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shahrekord, Iran

⁴ Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shahrekord, Iran

چکیده [English]

Introduction
Grapes (Vitis vinifera L.) belong to a group of temperate fruits, which have high nutritional qualities, and many by-products are produced from different parts of their berries. The difference in quantity and quality properties of different cultivars is an opportunity to screen grapes based on their desirable characteristics and nutritional value. The morphological and yield traits of grapes usually vary depending on the cultivar, vineyard climate, light, temperature, viticultural operations, nutrition, irrigation management, pests, disease control, pruning, training practices, and crop load. Since the study of the nutrients state can be a real criterion for evaluating the growth and yield of the plant, therefore, the present study was carried out to evaluate and compare the growth, yield and concentration of macro and micro nutrients elements in the leaves of 12 commercial varieties of grapes in the climatic conditions of Saman region located in Chaharmahal Bakhtiari province, Iran.
 
Materials and Methods
 The present study was conducted with the aim of investigating the morphological and pomological characteristics of 12 grape varieties in the grape collection garden of Chaharmahal province and Bakhtiari of Saman region in the base of a randomized complete block design with three replications. Saman region is located at 32.19° latitude, 50.51° longitude and 2060 meters above sea level. The studied area is a temperate mountainous region with an average temperature of 12.8°C and an average annual rainfall of 243 mm. The absolute maximum and absolute minimum temperature in the investigated garden are 42 and -32°C, respectively. In this research, three-year-old plants of Bidaneh-Ghermez, Flame, Perlette, Kashmiri 1, Shahroudi, Yaghouti, Kashmiri 2, Nasrallah daei's Asgari, Rish baba, Siahe-Mahali, Asgari, Siahe-Hasnabad were studied. In the fruit ripening stage, morphological traits, fruit yield and the concentration of nutrients in the leaves of the studied cultivars were measured in two growing seasons of 1400-1401 and 1402-1401.
 
Results and Discussion
 Having precise selection power among plants is necessary for breeding and production of new varieties, which depends on the identification of existing varieties and their diversity. In the present study most of the traits showed significant variations among the studied cultivars. The results revealed significant differences among the studied cultivars in terms of growth characteristics, including stem diameter, current season shoot length, number and spacing of internodes, and leaf surface area. Yield-related traits—such as cluster length, cluster width, cluster weight, berry length, and berry width—were also significantly influenced by the grape cultivar. The highest yield was observed in the Flame cultivar, producing 7.19 kg per plant. Based on the results, the evaluated grape cultivars were significantly differed from each other in terms of the concentration of macro and micro nutrient elements in the leaves. Correlation results showed that the leaf area, cluster characteristics, and the concentration of nitrogen, phosphorus, potassium, magnesium, iron, manganese, zinc, and boron significantly and positively correlated with the grape yield. Cluster analysis classified the studied grape cultivars into three groups, which were in accordance with the results of principal components analysis. Based on the results of this research, the cultivars Perlette, Flame, Bidaneh-Ghermez, Siahe-Hasnabad and Rish baba were superior to other cultivars in terms of the evaluated traits, which can be investigated for further studies and breeding programs. The amount of copper in the leaves of grape cultivars in the first year was higher than in the second year, and Asgari Dai Nasrallah and Siah Mahali cultivars had the highest (7.60 mg.kg^-1 in leaf fresh weight) and the lowest (4.28 mg.kg^-1) respectively. Kg.leaf fresh weight^-1) had copper content. The amount of manganese in the first year was more than the second year and the different grape cultivars were significantly different from each other. The highest amount of manganese was in Siahe-Hasnabad cultivars (95.92 mg.kg^-1 in leaf fresh weight) and Philim (93.50 mg.kg^-1 in leaf fresh weight) and the lowest amount in Kashmiri 2 variety (60.22 mg.kg^-1/leaf fresh weight) was measured. In the present study, the amount of zinc element measured in the first year was higher than the second year and it varied between 84.80 and 25.05 mg.kg^-1 in leaf fresh weight among different grape varieties. Fleim and Yaghuti cultivars had the highest and lowest amount of zinc element among the studied cultivars. Boron is one of the elements influencing the quantitative and qualitative characteristics of grapes, and the need for this element in grapes is more than other treasured fruit trees, and the presence of optimal amounts of boron is necessary for the formation of fruit and the growth and development of pods. Although the effect of the year on the boron content was not significant, its measured value was higher in the second year than in the first year. The highest (38.22 mg.kg^-1 in leaf fresh weight) and the lowest (17.62 mg.kg^-1 in leaf fresh weight) boron content were recorded in Pearlet and Kashmiri 2 cultivars, respectively. The recorded differences in the concentration of nutrients in the leaves of different grape cultivars can be attributed to several reasons: a) The ability to absorb nutrients may be different in the studied cultivars, so they act differently in the transfer and distribution of nutrients. b) Difference in the amount of active hormones in the root. c) Some nutrients are consumed in a larger amount by the roots, and as a result, the amount transferred to the branches and leaves decreases. The difference in the consumption of nutrients in the roots of different grape cultivars leads to the difference in the measured concentration of these elements in the leaves.
 
Conclusion
Significant differences were found among the 12 grape cultivars in terms of morphological, nutrient elements and yield traits. These findings confirmed that growth and berry yield of grape can be affected mainly by the cultivar-based genetic background. The results of this study can be used as a guide for selecting appropriate cultivars for further breeding programs.

کلیدواژه‌ها [English]

• Cluster analysis
• Commercial grape cultivars
• Correlation analysis
• Macronutrients
• Vine

©2025 The author(s). This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

مراجع

1. Abiri, K., Rezaei, M., Tahanian, H., Heidari, P., & Khadivi, A. (2020). Morphological and pomological variability of a grape (Vitis vinifera) germplasm collection, Scientia Horticulturae, 266, 109285. https://doi.org/10.1016/j.scienta.2020.109285
2. Akram, M.T., Qadri, R., Khan, M.A., Hafiz, I.A., Nisar, N., Khan, M.M., & Hussain, K. (2021). Morpho-phenological characterization of grape (Vitis vinifera) germplasm grown in northern zones of Punjab, Pakistan. Pakistan Journal of Agricultural Sciences, 58(4), 1223-1236. https://doi.org/10.21162/PAKJAS/21.91
3. Antolin, M.C., Toledo, M., Pascual, I., Irigoyen, J.J., & Goicoechea, N. (2020). The exploitation of local Vitis vinifera biodiversity as a valuable tool to cope with climate change maintaining berry quality. Plants, 10(1), 71. https://doi.org/10.3390/plants10010071
4. Bigard, A., Berhe, D.T., Maoddi, E., Sire, Y., Boursiquot, J.M., Ojeda, H., Péros, J.P., Doligez, A., Romieu, C., & Torregrosa, L. (2018). Vitis vinifera fruit diversity to breed varieties anticipating climate changes. Frontiers in Plant Science, 9, 455. https://doi.org/10.3389/fpls.2018.00455
5. Bodor-Pesti, P., Taranyi, D., Deák, T., Nyitrainé Sárdy, D.Á., & Varga, Z. (2023). A review of ampelometry: Morphometric characterization of the grape (Vitis) leaf. Plants, 12(3), 452. https://doi.org/10.3390/plants12030452
6. da Silva, M.J.R., Paiva, A.P.M., Junior, A.P., Sánchez, C.A.P.C., Callili, D., Moura, M.F., & Tecchio, M.A. (2018). Yield performance of new juice grape varieties grafted onto different rootstocks under tropical conditions. Scientia Horticulturae, 241, 194-200. https://doi.org/10.1016/j.scienta.2018.06.085
7. Doulati Baneh, H., & Nejatian, M.A. (2018). Adaptability and comparison of bunch and berry characteristic of some commercial foreign and Iranian grape cultivars in Urmia. Iranian Journal of Horticultural Science, 49(3), 681-692. (in Persian). https://doi.org/10.22059/ijhs.2017.231217.1237
8. Doulati Baneh, H., Taheri, M., Majidi, A., & Taheri, M. (2015). Amount of macronutrients and micronutrients in petiole of some Iranian and imported grape cultivars. Journal of Horticultural Science, 29(3), 397-405. (in Persian). https://doi.org/10.22067/jhorts4.v0i0.28029
9. Doulit Baneh, H. (2015). Grapes (Comprehensive Management of Cultivation, Breeding, Production and Processing). Kurdistan University Press, Sanandaj, Iran. 674 p. (in Persian)
10. Emami, A. (1996). Plant decomposition methods. Vol. 1. Technical leaflet No. 982. Soil and Water. Research Institute, Tehran, Iran. (in Persian)
11. Frioni, T., Bertoloni, G., Squeri, C., Garavani, A., Ronney, L., Poni, S., & Gatti, M. (2020). Biodiversity of local Vitis vinifera germplasm: A powerful tool toward adaptation to global warming and desired grape composition. Frontiers in Plant Science, 11, 608. https://doi.org/10.3389/fpls.2020.00608
12. Haddadinejad, M., Ebadi, A., Moghaddam, M.R.F., & Nejatian, M.A. (2013). Primary morphological screening of 698 grapevine genotypes to select drought tolerant rootstocks. Iranian Journal of Horticultural Science, 44(2), 193-207. (in Persian). https://doi.org/10.22059/ijhs.2013.35052
13. Jahnke, G., Nagy, Z.A., Koltai, G., Oláh, R., & Májer, J. (2021). Morphological, phenological and molecular diversity of woodland grape (Vitis sylvestris) genotypes from the Szigetköz, Hungary. Mitteilungen Klosterneuburg, 71, 90-98.
14. Karami, R. (2014). Evaluation morphological of turkmenistan and Iran cultivar grapevine and comparison of their vegetative and reprudactive traits. M.Sc Thesis in Persian Gulf University. Bushehr, Iran. 77 p.
15. Karimi, R., & Salimi, F. (2021). Iron-chlorosis tolerance screening of 12 commercial grapevine (Vitis vinifera) cultivars based on phytochemical indices. Scientia Horticulturae, 283, 110111, https://doi.org/10.1016/j.scienta.2021.110111
16. Karimi, R., & Koulivand. (2019). Soluble sugars, phenolic acids and antioxidant capacity of grape berries as affected by iron and nitrogen. Acta Physiologiae Plantarum, 41(7), 1-11. http://dx.doi.org/10.1007/s11738-019-2910-1
17. Kasnazany, S.A.S., Noori Mirza, A., Raouf Mahmood, A.M., Abdul Rahman, S.S., Shamsadden Hameed, J., & Nasrulla, K.B. (2023). A comparison of nine grape Vitis vinifera L. cultivars growing in Iraq’s Kurdistan region in terms of their vegetative growth and physicochemical traits. IOP Conference Series: Earth and Environmental Science, 1213, 012044. https://doi.org/10.1088/1755-1315/1213/1/012044
18. Kazemi, M., Rasouli, M., Maleki, M., Abdoli, M., & Rostami -Borujeni, M. (2022). Evaluation of phenotypic and genetic diversity of some native and foreign grapevine (Vitis vinifera L.) cultivars and genotypes based on morphological, phenological, biochemical and fruit characteristics (Case study: Khuzestan province, south-west of Iran). The 22^nd National and 10th International Congress on Biology, Shahrekord University, Shahrekord, Iran. 31 August to 2 September 2022.
19. Khandani, Y., Gholami, M., Sarikhani, H., & Chehregani Rad, A. (2022). Response of some vegetative and physiological traits of Iranian and foreign grape cultivars to drought stress. Plant Process and Function, 11(51), 10. http://dorl.net/dor/20.1001.1.23222727.1401.11.51.10.7
20. Leão, P.C.D.S., & Oliveira, C.R.S.D. (2023). Agronomic performance of table grape cultivars affected by rootstocks in semi-arid conditions. Bragantia, 82, e20220176. https://doi.org/10.1590/1678-4499.20220176
21. Lindsay, W.L., & Norvell, W.A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal, 42, 421-428. https://doi.org/10.2136/sssaj1978.03615995004200030009x
22. Mirfatah, S.M.M., Rasouli, M., Gholami, M., & Mirzakhani, A. (2024). Phenotypic diversity of some Iranian grape cultivars and genotypes (Vitis vinifera) using morpho-phenological, bunch and berry traits. Journal of Horticulture and Postharvest Research, 7(2), 115-140. https://doi.org/10.22077/jhpr.2024.7165.1355
23. Rasoli, V. (2023). Yield stability of fifteen foreign introduced grapevine varieties in deficit irrigation conditions. Research in Horticultural Sciences, 1(2), 241-252. (in Persian). https://doi.org/10.22092/rhsj.2023.129172
24. Rasoli, Y., Ghaderi, N., & Khadivi, A. (2017). Morphological variation and marker-fruit trait associations in a collection of grape (Vitis vinifera). Scientia Horticulturae, 225, 771-782. https://doi.org/10.1016/j.scienta.2017.08.007
25. Rasouli, M., & Kalvandi, Z. (2022). Investigating the morphological and pomological diversity of some grape cultivars and genotypes collected from different regions of Iran. The First National Conference on Production and PostHarvest Technology of Horticultural Plants, Birjand University, Birjand, Iran. https://civilica.com/doc/1533228
26. Razi, M., Darvishzadeh, R., Doulati Baneh, H., Amiri, M.E., & Martinez-Gomez, P. (2021). Estimating breeding value of pomological traits in grape cultivars based on REMAP molecular markers. Plant Productions, 44(4), 515-530. https://doi.org/10.22055/ppd.2020.34003.1925
27. Schaller, K., & Löhnertz, O. (1990). Investigations on the nutrient uptake efficiency of different grape root-stock species and cultivars. In: El Bassam, N., Dambroth, M., & Loughman, B.C. (eds) Genetic Aspects of Plant Mineral Nutrition. Developments in Plant and Soil Sciences, vol 42. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2053-8_13
28. Szűgyi-Reiczigel, Z., Ladányi, M., Bisztray, G.D., Varga, Z., & Bodor-Pesti, P. (2022). Morphological traits evaluated with random forest method explains natural classification of grapevine (Vitis vinifera) cultivars. Plants, 11, 3428. https://doi.org/10.3390/plants11243428
29. Villano, C., Corrado, G., Basile, B., Di Serio, E., Mataffo, A., Ferrara, E., & Aversano, R. (2023). Morphological and genetic clonal diversity within the ‘Greco Bianco’grapevine (Vitis vinifera) variety. Plants, 12(3), 515. http://dx.doi.org/10.3390/plants12030515
30. Vivin, P., Lebon, E., Dai, Z.W., Duchêne, E., & Marguerit, E. (2017). Combining ecophysiological models and genetic analysis: A promising way to dissect complex adaptive traits in grapevine. Oeno One, 51, 181–189. https://doi.org/10.20870/oeno-one.2017.51.2.1588
31. Zhou, Y., Massonnet, M., Sanjak, J., Cantu, D., & Gaut, B.S. (2017). The evolutionary genomics of grape (Vitis vinifera vinifera) domestication. Proceedings of the National Academy of Sciences, 114, 11715–11720. http://dx.doi.org/10.1073/pnas.1709257114