Study on Freezing Tolerance of Calendula (Calendula officinalis L.) in Vegetative and Reproductive Stages

Document Type : Research Article

Authors

• A. Nezami ¹
• Seyed Mmohammad javad Mousavi ²
• S. Nezami ¹
• E. Izadi Darbandi ¹
• M. Yousef Sani ¹
• fatemeh keykha akhar

¹ Ferdowsi University of Mashhad

² Lecture , Department of Horticulture Sciences, Faculty of Agricultural, Ferdowsi University of Mashhad

10.22067/jhorts4.v0i0.42859

Abstract

Calendula (Calendula officinalis) is relatively cold tolerant plant, but in some years plant seriously injured due to harsh winter. In order to evaluate freezing tolerance of calendula an experiment was carried out at college of Agriculture, Ferdowsi University of Mashhadin a factorial-completely randomized design with three replications and plants of two sowing dates (summer and autumn) were exposed to12 temperatures (0, -2, -4, -6, -8, -10, -12, 14- ,16-, -18, -20 and -22oC). Seeds of calendula plants were sown in summer (summer plant) and autumn (autumn plant) in the bed and in six to eight-leaf stages were transplanted to the pots. After the cold acclimation in natural condition, freezing stress was applied with using a thermo gradient freezer. To employ stability of cytoplasmic membrane, percentage of electrolyte leakage (EL%) was measured after freezing. Also survival percentage (Su%) and regrowth of calendula plants determined after three weeks recovery. Leaves EL% in autumn plants was significantly more than summer plants and autumn plants have higher Su%, but plant height, number of lateral branches, numbers of reproductive traits, total dry matter, vegetative and reproductive dry matter in summer plants were more than autumn plants. However, there were no difference between calendula plants for LT50el in both autumn and summer plants, but there was significant difference between them for LT50su and total dry matter, and LT50su and reduced dry matter temperature50 (RDMT50)for summer plants were -18.6 oC and -11.3 oC and for autumn plants were -19.4 oC and -13.7 oC, respectively.

Keywords

• Dry matter
• Electrolyte leakage
• Lateral stem
• Plant height
• Survival percentage

20.1001.1.20084730.1393.28.3.11.6

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References

- باقری ع.، نظامی ا.، و سلطانی م. 1379. اصلاح حبوبات سرما دوست برای تحمل به تنش‌ها. انتشارات سازمان تحقیقات، آموزش و ترویج کشاورزی. مشهد.

2- توکل م.س. 1387. فنولوژی و استقرار گیاهان دارویی و معطر در باغ گیاهشناسی ملی ایران. انتشارات موسسه تحقیقات جنگل‌ها و مراتع کشور. تهران.

3-جوادموسوی م.، نظامی س.، ایزدی دربندی ا.، نظامی ا.، یوسف ثانی م. و کیخاآخر ف. 1390. مطالعه اثرات تنش یخ زدگی بر گیاه مینای چمنی (Bellis perennis) در شرایط کنترل شده. نشریه آب و خاک (علوم و صنایع غذایی)، 25 (2): 380-388.

4- خلیقی ا. 1376. گلکاری. انتشارات گلشن. تهران.

5-دوازده امامی س.، سفیدکن ف.، جهانسوز م.ر.، و مظاهری د. 1387. مقایسه عملکرد بیولوژیکی، عملکرد کمی و کیفی اسانس و مراحل فنولوژیکی در کشت پاییزه، بهاره و تابستانه بادرشبویه (Dracocephalum moldavica L.). فصلنامةعلمی-پژوهشی تحقیقات گیاهان دارویی و معطر ایران، 24 (3): 263-270.

6- نظامی ا.، کیخاآخر ف.، جوادموسوی م.، ایزدی دربندی ا.، نظامی س و یوسف ثانی م. 1390. اثر تنش یخ زدگی بر گیاه بنفشه Viola gracilis L.)) تحت شرایط آزمایشگاهی. مجله بوم شناسی، 3 (4): 430-438.

7-Adkins J.K., Dirr M.A., and Lindstorm O.M. 2002.Cold hardiness potential of ten Hydrangea taxa. Journal of Environment and Horticulture, 20(3): 171–174.

8-Anderson J.A., Michael P., and Taliaferro C.M. 1988.Cold hardiness of Midiron and Tifgreen. Horticultural Science, 23: 748-750.

9-Annicchiarico P., and Iannucci A. 2007. Winter survival of pea, faba bean and white lupin cultivars in contrasting Italian locations and sowing times, and implications for selection. Journal of Agricultural Science, 145: 611–622.

10-Azzaz N.A., Hassan E.A., and Elemarey F.A. 2007. Pysiological, anatomical, and biochemical studies on pot marigold (Calendula officinalis L.) plants. African Crop Science Conference Proceedings, 8: 1727-1738.

11-Byard S., Wisniewski M., Li J., and Karlson D. 2010.Interspecific analysis of xylem freezing responses in acer and betula. Horticultural Science, 45 (1): 165-168.

12-Cardona C.A., Duncan R.R., and Lindstrom O. 1997.Low temperature tolerance assessment in paspalum. Crop Science, 37: 1283-1291.

13-Ebrahimi A., Moaveni P., and Aliabadi Farahani H. 2011.Effects of planting dates and compost on mucilage variations in borage (Borago officinalis L.) under different chemical fertilization systems. International Journal of Biotechnology, 2(1): 194-197.

14-Gusta L.V., Fowler D.B., and Tyler N.J. 1982.Factors influencing hardening and survival in winter wheat. In: Li P. H., and Sakai A. (eds.). Plant Cold Hardiness and Freezing Stress.Academic press. New York.

15-Gusta L.V., O’Connor B.J., Gao Y.P., and Jana S. 2001. A re-evaluation of controlledfreeze-test and controlled environment hardening conditions to estimate the winter survival potential of winter wheats. Canadian Journal of Plant Science, 81:241-246.

16-Huyghe C.1991. Winter growth of autumn-sown white lupin (Lupinus albus L.): main apex growth model. Annals of Botany, 67: 429-434.

17-Iles J.K., and Howard Agnew N. 1993. Determining cold hardiness of Heuchera sanguine Engelm.‘chatterbox’ using dormant crowns. Horticultural Science, 28(11):1087–1088.

18-Junttila O., and Robberecht R. 1993. The influence of season and phenology on freezing tolerance in Silen acaulis L., a subarctic and arctic cushion plant of circumpolar distribution. Annals of Botany, 71: 423-426.

19-Kim D.C., and Anderson N.O. 2006.Comparative analysis of laboratory freezing methods to establish cold tolerance of detached rhizomes and intact crowns in garden chrysanthemums (Dendranthema- grandiflora Tzvelv.). Scientia Horticulturae, 109: 345–352.

20-Link W., Balko C., and Stoddard F.L. 2008. Winter hardiness in faba bean: Physiology and breeding. Field Crops Research, 115: 287–296.

21-Nezami A., Manjula S.B., and Gusta L.V. 2012. An evaluation of freezing tolerance of winter chickpea (Cicer arientinum L.) using controlled freeze tests. Canadian Journal of Plant science, 92: 155-161.

22-Nishida T., Kitagawa M. and Yamamoto Y. 2004: Effect of sowing date on overwintering and freezing tolerance of horsenettle (Solanum carolinense L.) seedlings. Grassland Science, 50(2): 139-146

23-Pennycooke J.C., Cox S., and Stushnoff C. 2005.Relationship of cold acclimation, total phenolic Content and antioxidant capacity with chilling tolerance in petunia (Petunia × hybrida). Environmental and Experimental Botany, 53: 225–232.

24-Perry L.P., and Herrick T. 1996. Freezing date and duration Effects on regrowth of three species of container-grown herbaceous perennials. Journal of Environment and Horticulture, 14 (4): 214-216.

25-Pietsch G.M., Anderson N.O., and Li P.H. 2009.Cold tolerance and short day acclimation in perennial Gaura coccinea and G. drummondii. Scientia Horticulturae, 120: 418–425.

26-Rashed Mohasel M.H., Nezami A., Bagheri A., Hajmohammadnia K., and Bannayan M. 2009. Evaluation of freezing tolerance of two fennel (Foeniculum vulgar L.) ecotypes under controlled conditions. Journal of Herbs, Spices & Medicinal Plants, 15:131–140.

27-Roland L.J., Dhanaraj A.L., Naik D., Alkharouf N., Matthews B. and Arora R. 2008. Study of cold tolerance in blueberry using EST libraries, cDNA microarrays, and subtractive hybridization. Horticultural Science, 43(7): 1975-1981.

28-Stitt M., and Hurry V. 2002. A plant for all seasons: alterations in photosynthetic carbon metabolism during cold acclimation in Arabidopsis. Physiology and Metabolism, 5:199-206.

29-Teutonico R.A., Palta J.P., and Osborn T.C. 1993.In vitro freezing tolerance in relation to winter survival of rapeseed cultivars. Crop Science, 33: 103-107.