Growing vegetables
J. Panahandeh; M.S. Zare far; A. Motallebi-Azar; F. Zare Nahandi; M. Amani
Abstract
IntroductionVarious methods of stress directly affected the growth and production yield of numerous plants. For example, environmental stress reduces the tomato manufacturing by the disrupting its natural metabolism, or, salinity stresses affect the it is growth and development from the germination stage ...
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IntroductionVarious methods of stress directly affected the growth and production yield of numerous plants. For example, environmental stress reduces the tomato manufacturing by the disrupting its natural metabolism, or, salinity stresses affect the it is growth and development from the germination stage to the fruit ripening stage. Salinity in tomatoes by stimulating the biosynthesis of growth regulators such as ethylene and abscisic acid leads to the acceleration of the aging of the leaves. Therefore, development of different methods to induce salinity stress tolerance in plants is necessary. Some approaches were studied to develop the salinity tolerant plants such as genetic breeding, environmental improvements and usage of phytohormones and signal molecules. Salicylic acid or orthohydroxybenzoic acid plays an important role in regulating the physiological and biochemical responses of plants to stress conditions, which improves the plant's resistance to adverse environmental conditions. For instance, salicylic acid is a facile and effective way to increase plant productivity under salt stress conditions. Considering the positive effects of salicylic acid in modulating the effects of salinity, this study was conducted with the aim of investigating the effects of salicylic acid’s usage in modulating the harmful effects of salinity on some vegetative, physiological, quantitative and qualitative characteristics of two tomato cultivars of Baneh local mass and Semi Dwarf line.Materials and MethodsTo investigate the effect of salicylic acid in modulating the effects of salinity stress in tomato, a factorial experiment was conducted in the form of a randomized complete block design, with 12 treatments, in 3 replications and with a total of 36 experimental units in the hydroponic greenhouse of the Department of Horticulture, Faculty of Agriculture, and university of Tabriz. The treatments included two levels of salicylic acid (0 and 1 mM) and salinity levels (0, 35 and 70 mM NaCl) on two tomato cultivars of Baneh and Semi Dwarf.Results and DiscussionThe results showed that in Baneh and Semi Dwarf cultivars, the increase in salinity levels caused a decrease in vegetative indices, meanwhile the treatment of salicylic acid along with salt stress increased same indices. Also, salt stress caused yield reduction in both Baneh and Semi Dwarf cultivars. By examining the qualitative indicators, it was observed that titratable acidity and vitamin C increased with salt stress and salicylic acid treatment in both cultivars. In terms of physiological indicators, the amount of proline increased at different salinity levels with salicylic acid treatment, but the amount of leaf chlorophyll index decreased with the increase of same condition.ConclusionThe results of testing the effect of salicylic acid and the effects of salinity stress on vegetative, quantitative, qualitative and physiological indicators in Baneh and Semi Dwarf tomatoes showed a remarkable difference in terms of significance. In terms of vegetative traits; Plant height, leaf area index, shoot wet in Baneh and Semi Dwarf cultivars decreased with increasing salinity levels of vegetative indices, but salicylic acid treatment along with salinity stress increased same indices. Indicators such as yield, fresh weight of fruit, and percentage of dry matter of fruit showed different responses to different levels of salinity and salicylic acid treatment. The fresh weight of fruit increased with the application of salicylic acid. Also, salt stress caused an increase in the percentage of dry matter of the fruit. But salt stress caused yield reduction in both Baneh and Semi Dwarf cultivars. In terms of quality indicators; the amount of titratable acidity and vitamin C increased with salt stress and salicylic acid treatment in both cultivars. In terms of physiological indicators, the level of proline increased across various salinity levels with salicylic acid treatment. However, the leaf chlorophyll index decreased with rising salinity levels, even in the presence of salicylic acid treatment. Overall, salinity stress caused a decrease in most analyzed traits in the Baneh and Semi Dwarf cultivars. Nevertheless, it led to improvements in certain quality traits. Additionally, salicylic acid treatment enhanced the mentioned indices in most of the examined traits in both cultivars. Therefore, considering the positive effects of salicylic acid treatment on Baneh and Semi Dwarf cultivars under salinity stress conditions, its use is recommended.
Growing vegetables
Gh. Kiani; S. Golcheshmeh
Abstract
Introduction
Tomato is a self-pollinated crop and has a high potential for heterosis production. Tomato has a wide range of diversity in terms of vegetative and fruit traits. Therefore, learning information about the genetics of the tomato plant and the inheritance of its various traits to the next ...
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Introduction
Tomato is a self-pollinated crop and has a high potential for heterosis production. Tomato has a wide range of diversity in terms of vegetative and fruit traits. Therefore, learning information about the genetics of the tomato plant and the inheritance of its various traits to the next generation will help plant breeders to use appropriate breeding methods to improve them. One of the methods that is used to know the genetic structure of plants, identify parental lines and determine their combining ability is line × tester analysis. Line × tester analysis provides information about general and specific combining of parents and can be useful in estimating different types of gene effects such as additive and non-additive effects. In most of the developed countries, many researches have been done in relation to hybrid production and combining ability among tomato lines, and sometimes the inferred results are different from each other. In Iran, few studies have been done about crossing cultivars and their hybrids, and most of the seeds used by farmers are imported from other countries. Therefore, this study intends to evaluate genetic variance components, general and specific combining ability of some quantitative traits in a number of tomato lines and testers and their hybrids by using line × tester analysis.
Materials and Methods
This research was conducted in Sari Agricultural Sciences and Natural Resources University, Mazandaran Province, Iran in 2022. Two modified cultivars SC and V as lines and three modified cultivars L, R and MZ as testers were crossed with each other to create F1 hybrids. Six F1 genotypes and their parents (11 treatments in total) were cultivated in the farm in a randomized complete block design with three replications. The evaluated traits included the number of days to the first flowering, earliness, number of fruits per plant, fruit weight per plant (g), fruit yield (g), fruit length and width (cm). In order to analyze the variance of the experimental design to search for diversity between treatments, to separate the effects of treatments into their components based on line × tester analysis, to mean comparison with Duncan's test, and also to calculate the general and specific combining ability, R statistical software was used. Also, in order to calculate additive and non-additive variances, Singh and Chaudhary's method was used.
Results and Discussion
The results of line × tester variance analysis showed that the mean squares of parents and testers were significant for all traits except fruit length and width, and the mean squares of crosses and lines were significant for all traits except fruit length. The effect of line × tester was significant for all traits except the number of fruits per plant and fruit length. The line of SC to improve the number of days to first flowering, earliness, plant height, fruit weight per plant, and fruit width, and the line of V to improve the number of fruit per plant were the best general combiners with testers. The tester of L for improve all traits except yield, and the tester of MZ for improve plant height were the best general combiners with the maternal lines. Among the crosses, the SC×L cross for improve earliness and fruit width, and the SC×R and V×MZ crosses for improve plant height and fruit weight per plant, respectively, were favorable specific combiners. The mean comparison of the genotypes for some important traits showed that among the parental cultivars, the line of SC and among the crosses, the SC×L genotype had the lowest means for the number of days to first flowering and earliness. Also, the line of SC for the number of fruits per plant and the SC×L genotype for fruit weight per plant, yield and fruit width had the highest means. Also, the estimation of additive and non-additive variances indicated that in plant height and fruit weight per plant traits, additive variance plays the main role. While for the traits of the number of days to first flowering, earliness and yield, the contribution of non-additive variance was more than the additive variance.
Conclusion
According to the results obtained from this study, in future projects it is recommended to use parents that have significant general combining ability (GCA) for traits. Because such parents easily transfer the trait to their next generation. In this way, the line of SC was a good general combiner for the number of days to first flowering, earliness, plant height, fruit weight per plant and fruit width, and the line of V was a good general combiner for the number of fruits per plant. Among the testers, the tester of L was a good general combiner for improve the number of days to first flowering, earliness, number of fruits per plant, fruit weight per plant, and fruit width, and the tester of MZ recorded a high GCA for the plant height. Also, for the improvement of earliness and fruit width, the SC×L cross and for plant height and fruit weight per plant, SC×R and V×MZ crosses were favorable specific combiner. Mean comparison of genotypes showed that the SC×L cross is superior to its parents for the number of days to first flowering, earliness, fruit weight per plant, fruit yield, and fruit length and width. The traits of plant height and fruit weight per plant are more affected by additive variance, so the best breeding method to improve plant height and fruit weight per plant is selection from among the segregating population. The traits of number of days to first flowering, earliness and yield were affected by non-additive variance, so hybrid production is recommended to improve the mentioned traits.
Growing vegetables
A. Rezvan; S.A. Eftekhari; R. Salehi; F. Sedighi Dehkordi
Abstract
Introduction: Cherry tomatoes, all over the world, have become a popular product because of the favorable characteristics (a good source of vitamins A and C, soluble solids, flavor, low calorie and fruit formation at high temperature). Cultivar selection is one of the most important managerial decisions ...
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Introduction: Cherry tomatoes, all over the world, have become a popular product because of the favorable characteristics (a good source of vitamins A and C, soluble solids, flavor, low calorie and fruit formation at high temperature). Cultivar selection is one of the most important managerial decisions and, despite thousands of available varieties, it can be daunting task. Cultivars are different in attributes such as color, shape, size, taste, taste, growth, consumption, planting time, and resistance to pests and disease.Material and Methods: This study was carried out from the autumn of 2014 to spring of 2015, at the research greenhouses of the University of Shahid Chamran, Ahvaz, Iran. The study was performed in randomized complete block design with three replications. During the cultivation period and at the end of the experiment, the number of clusters in the plant, the number of fruits in the cluster, the number of fruits in the plant, the total function in the bush , the number of marketable and unmarketable fruits in plant, the harvest index, vitamin C and soluble solids were evaluated. In addition, nitrate content, nitrite, fruit lycopene and photosynthetic pigments (chlorophyll a, chlorophyll b, chlorophyll a to b ratio, total chlorophyll, carotenoid and chlorophyll to carotenoid ratio) and the SPAD index were measured.Results and Discussion: According to the comparison of the means, there were the highest number of clusters in Baby Tom (16/44) and then in Belize (15/88), which were not statistically significant and the lowest number of clusters were observed in cultivar Sogno (12.77). The highest and lowest number of fruits in the cluster were found in the cultivars of Sogno and Belize (29.82 and 17.73), respectively. The highest number of fruits in the plant (295) were observed in Sogno and the Belize cultivar had the lowest number of fruits (193.56). The highest average of single fruit weight related to the Cherry Belle (11.13 g) and the lowest in Sogno (8 g) were observed. The lowest and the maximum percentage of the unmarketable fruits were obtained in Baby Tom (09/09 %) and Sogno (32 %), respectively. The maximum yield was observed in the Cherry Belle (2929 g) and then in Sogno (2482.4 g) and Baby Tom (2077.6 g) and the minimum yield (1637.7 g) in the Belize cultivar. The highest and lowest marketable yield were obtained in Belle (2928.6 g per plant) and Belize (1636.6 g per plant) cultivars, respectively. The maximum amount of soluble solids was observed in Baby Tom (4.86 °Brix), which had no statistically significant differences with the Belle and Sogno and the lowest soluble solids was found in Belize (3.15 °Brix). There were the highest and lowest vitamin C content in Cherry Belle (28.24 mg per 100 g FW) and Belize, respectively (23.1 mg in 100 g FW). According to the results, the maximum and lowest content of lycopene were observed in the Cherry Belle and Baby Tom, with an average of 32.411 and 19.402 mg/kg, respectively. The maximum content of nitrate in fruits was observed with 0.67 mg/gr DW in the fourth cluster and the lowest content of nitrate with 0.198 mg/gr DW in the tenth cluster. The highest indices of leaf SPAD were found in Belize (28.9) and lowest in Cherry Belle (16.32).Conclusion: The selection of high - yield or high - quality cultivars in a greenhouse culture is critical to the economic efficiency of this product. The difference in yield is mainly related to the genetic variation among varieties. Study of yield and yield components of four samples of tomato in the environment and similar culture showed that the Belle Cherry was the best result for yield, harvest index, and vitamin C, so this cultivar is recommended to grow in Ahvaz greenhouses.
S. Khezerloo; B. Abdollahi Mandoulakani
Abstract
Introduction: Commercial tomato (Solanum lycopersicum L.), one of the most widely grown vegetable crops worldwide, belongs to the Solanaceae family. The marketability of the commercial tomato mostly depends on the fruit quality. Tomato fruit quality is determined mainly by color, texture, shape and flavor. ...
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Introduction: Commercial tomato (Solanum lycopersicum L.), one of the most widely grown vegetable crops worldwide, belongs to the Solanaceae family. The marketability of the commercial tomato mostly depends on the fruit quality. Tomato fruit quality is determined mainly by color, texture, shape and flavor. Fruit shape, one of the important traits affecting the quality of tomato fruit, is controlled by multiple minor genes and quantitatively inherited. Two important genes, involved in fruit shape, are SUN and OVATE genes. The SUN gene, which is a member of the IQD (IQ-domain) gene family and the Calmodulin binding protein, controls fruit length. The more expression of both SUN and OVATE genes leads to increased fruit length. Moreover, the increased expression of OVATE gene reduces the size of flower and leaf components. Due to the important role of these genes in tomato fruit shape, identification of single nucleotide polymorphisms (SNPs) as a new generation of robust, frequent and reliable bi-allelic markers, in the coding regions of these genes might be necessary for generating functional markers associated with fruit shape. Materials and Methods: Seeds of 96 tomato genotypes from 12 populations were grown in the research greenhouse of Faculty of Agriculture and Natural Resources of Urmia University. The genotypes had been collected from different regions of West Azerbaijan of Iran and Turkey (Iğdır). The young and green plant leaves were used for genomic DNA extraction. The quality and quantity of the extracted DNA was assessed using spectrophotometry and agarose gel electrophoresis. To identify SNPs in SUN and OVATE genes, specific primers were designed by using FastPCR and Gene Runner software for amplifying fragments from coding regions of these genes in 96 tomato genotypes. Then, the amplified fragments of both genes were digested by using restriction enzymes TruI and PstI. Due to the lack of polymorphism in the digested patterns obtained by the used enzymes, four individuals from populations with close geographical distance were selected and amplified. The amplified bands were then purified by a purification kit (Kiagen, USA) and sequenced (Bioneer, South Korea). Sequencing was performed from both ends of the PCR fragments using both the forward and reverse primers used in the PCR reactions. The exon and intron regions of the sequenced fragments were identified by Softberry software. Following the retrieval of the sequenced fragments of each gene using FastPCR and Softberry software, multiple sequence alignment using Clustal Omega was used to identify SNPs in the exon and intron of the genes. Results and Discussion: Digestion of the amplified fragments of the genes using TruI and PstI restriction enzymes produced no polymorphism in the studied genotypes. Thus, four individuals were selected from geographically different populations and gene fragments were amplified, purified and sequenced in these genotypes. Sequencing of the amplified fragment of SUN gene revealed an intron region with a size of 369 bp. Out of the 10 SNPs detected in the SUN gene, four was found in the exon region, while the number of SNPs in intron was six. Of the total SNPs found in the SUN gene, the percentage of transition and transversion substitutions was 80 (50% T/C and 30% A/G) and 20 (T/G), respectively. In the OVATE gene, five SNPs were identified. The percentage of transition (40% G/A and 40% C/T) and transversion (20% G/T) substitutions in this genes were the same as SUN. The ratio of transition to transversion substitutions was 1:4 for both genes. The average number of SNPs in a 100 bp fragment in exonic and itronic region of SUN was 0.9 and 1.62, respectively, while it was 0.5 for exonic region of OVATE gene. Conclusion: The results of the current study revealed low polymorphisms and point mutations in the exon regions of SUN and OVATE genes, suggesting that the coding regions of these genes were conserved during the tomato evolution. Also, the number of SNPs in intron was more than those of exon. Considering the important role of fruit quality, especially fruit shape, in tomato market, the SNPs found in the current study may be used in genetic diversity studies, genetic map preparation, and saturation and identification of the functional markers associated with tomato fruit shape. These markers could accelerate tomato breeding programs aimed fruit shape improvement.
Growing vegetables
M. Zamanipour
Abstract
Introduction: Tomato (Solanum lycopersicum L.) is a perennial plant, which is rich in antioxidant compounds, lycopene, polyphenols and vitamin C. Iran, with production of 5.24 million tons, is ranked sixth in the world in tomato production. According to the latest FAO reports in 2019, the total area ...
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Introduction: Tomato (Solanum lycopersicum L.) is a perennial plant, which is rich in antioxidant compounds, lycopene, polyphenols and vitamin C. Iran, with production of 5.24 million tons, is ranked sixth in the world in tomato production. According to the latest FAO reports in 2019, the total area under tomato cultivation was 121203 hectares, with an average yield of 43.30 tons per hectare, and annual production of 5248904 tons. Vitamins are made from natural ingredients and are suitable for the growth, function and improvement of plant nutrition. The aim of this study was to investigate the effects of different levels of pyridoxine (50, 100 and 150 mgL-1), thiamine (50, 100 and 150 mgL-1) and folic acid (50, 100 and 150 mgL-1) and the combination of these vitamins on the plant growth, yield and chemical properties of tomatoes.
Materials and Methods: This study was conducted as randomized complete block design with three replications in the greenhouse of Iranshahr University during the years 2019 to 2020. The tomato cultivar was Delphus, the seedling of which was purchased from Pakan Bazr Isfahan Company. In August, with the beginning of the tomato planting period in the greenhouse, seedlings were planted and the harvest lasted until December. Seedlings were planted in rows of 75 cm wide and 40 cm apart. Irrigation was performed in the greenhouse with a drip system. The first irrigation was carried out immediately after planting and the second and third irrigations were carried out one day later for one hour and the subsequent irrigations were carried out in proportion to the growth of seedlings, every other day, every four days. At the 7-8 leaf stage, the plants were guided vertically on the thread. The greenhouse temperature was 25 to 32 °C during the experiment and 18 to 24 °C at night and the relative humidity was about 50%.
Results and Discussion: The results showed that all used concentrations of pyridoxine, thiamine and folic acid increased the growth parameters compared to the control, so that the highest plant height (271 cm), stem diameter (7 cm), number of leaves (31) fresh weight (502 g) and dry weight (341.66 g) were produced at a concentration of 100 mgL-1 pyridoxine + 100 mgL-1 thiamine + 100 mgL-1 folic acid. Interaction of B vitamin levels at low, medium and high levels had a significant effect on the reproductive parameters of tomato plants, so that the highest number of flowers (41.33), number of fruits (29.55), number of clusters (9.77), fruit diameter (22.44 mm), fruit fresh weight (158 g) and fruit dry weight (10.81 g) and yield (5688.9667 g/plant) at a concentration of 100 mgL-1 pyridoxine, 100 mgL-1 thiamine and 100 mgL-1 was observed per liter of folic acid. Increasing of yield can be due to increased nutrient uptake and assimilation, and increased growth due to the presence of vitamins. Similar results by El-Gharmany et al. (2005) stated that foliar application of vitamins (B1, B6 and B12) in appropriate concentrations in cowpea significantly increased the number of pods per plant and total yield compared to the control. Shabaly and El-Ramady (2014) and Shabana et al. (2015) found that some natural ingredients have increased yield of garlic and tomatoes. Also, all concentrations of pyridoxine, thiamine and folic acid used increased biochemical parameters compared to the control. Maximum pH (4.78), acidity (0.28%), soluble solids (3.93%), lycopene (2.64 mg/100 g fresh weight), total phenol content (66.66 mg/100 g fresh weight, vitamin C (13.36 mg/100 g fresh weight), chlorophyll a (1.98 mg/g fresh weight), chlorophyll b (0.98 mg /g fresh weight) and carotenoids (3.33 mg/g fresh weight) were obtained by using a combination of 100 mgL-1 pyridoxine, 100 mgL-1 thiamine and 100 mgL-1 folic acid. Foliar application of vitamin treatments may play an important role in physiological and metabolic processes that affect the process of photosynthetic metabolism and lead to an increase in soluble solids and minerals. The interaction of vitamins improves the action of biochemicals on amino acid metabolism and nucleic acid synthesis. However, Abdel-Halim (1995) reported that foliar application of some vitamins improved leaf growth, increased chlorophyll, chemicals, and internal hormones in tomatoes during the winter. El-Ghamriny (2005) reported that foliar application of B vitamins (B1, B6 and B12) increased leaf chlorophyll in cowpea compared to the control, and Burguieres et al. (2007) found that folic acid at a concentration of 50 mgL-1 increased minerals in peas. Hendawy and Ezz El-Dinn (2010) reported that vitamin B complex as a coenzyme in enzymatic reactions such as carbohydrates, fatty acids and proteins involved in photosynthesis and respiration. In addition, Abd El-Hakim (2006) reported that some antioxidants improve biochemical properties in some beans.
Conclusion: The results showed that the use of pyridoxine, thiamine and folic acid vitamins alone or in combination with each other improved the growth, reproductive and biochemical characteristics of Delphi greenhouse tomatoes. The highest growth rate, yield and biochemical properties were obtained at 100 mgL-1 pyridoxine + 100 mgL-1 thiamine + 100 mgL-1 folic acid.
Postharvest physiology
S. Aghaei Dargiri; D. Samsampour; M. Askari Seyahooei; A. Bagheri
Abstract
Introduction: Tomato (Solanum lycopersicum L.) is a common vegetable that is widely cultivated and consumed worldwide in the Solanaceae family (global tomato production is estimated at approximately 182 million tons in 2017). Tomato, because of its elevated nutritional value, is the second most common ...
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Introduction: Tomato (Solanum lycopersicum L.) is a common vegetable that is widely cultivated and consumed worldwide in the Solanaceae family (global tomato production is estimated at approximately 182 million tons in 2017). Tomato, because of its elevated nutritional value, is the second most common vegetable commodity in the world after potatoes. As with other crops, the global production of tomatoes is threatened by certain biological stresses (such as pests, plant diseases and weeds) and non-biological stresses (such as salinity, drought, floods, cold and heat stress). Nowadays, the excessive use of chemical fertilizers in tomato production in order to increase yields, has resulted in environmental pollution and dangers on the health of consumers. The reaction of cultivated plants to these challenges is indicated by numerous morphological, physiological, biochemical and molecular changes, leading to a direct and indirect decrease in plant growth and productivity. Salinity as a non-biological stress can cause osmotic or ionic imbalance in plant cells. Salinity stress also limits growth and germination by affecting water and reducing water availability and affects crop production. Endophytes represent an eco-friendly option for the promotion of plant growth and for serving as sustainable resources of novel bioactive natural products. One of the alternative ways to restore normal plant growth under salinity stress may be to use plant growth to stimulate endophytes. Endophytes can play an important role in plant survival under salinity stress by reducing the adverse effects of sodium ion. Therefore, this work provides strong evidence that endophyte halophyte can be beneficial for tomato that help tolerate the plants stress. Materials and Methods: The main aim of this study was to investigate the role of endophytic bacteria (Exigubacterium aurantiacum), isolated from Salsola imbricate, in improving the growth of Solanum lycopersicum L. (8320) under salinity stress. The salinity tolerance potential of bacterial endophytes was investigated in vitro. The bacterial was cultured in Nutrient Agar with different concentrations of NaCl (1, 2 and 3 M) and its growth dynamics were investigated after 24 and 120 hours. To prepare the bacterial suspension for inoculation with tomato seeds, the bacteria were cultured on NB (Nutrient Broth) medium for 24 hours in an incubator at 28±1 °C at 130 rpm. The OD suspension was adjusted to a concentration of 1×108 ml. Tomato seeds (cultivar 8320) were washed with ethanol (70%) for 30 seconds and then sterilized with 0.5% sodium hypochlorite for 90 minutes and then completely distilled three times with distilled water. They were autoclaved and washed. For better contact of seeds with bacteria, 1% carboxymethylcellulose was used and then the seeds inoculated with bacterial treatments were placed on a shaker for six hours. Seeds inoculated with bacterial endophytes were planted in seedlings and then placed in pots containing autoclaved soil in the greenhouse of the Faculty of Agriculture, Hormoz University. The experiment was arranged in a factorial experiment based on randomized complete block design with three replications. Experimental treatments included five levels of salinity stress (0, 4, 6, 8 and 10 dS/m-1) and bacterial endophyte inoculation (E. aurantiacum). Analysis of variance of traits was performed using SAS software version 9.4 and the means were compared using LSD method with a probability level of p < /em> Results and Discussion: Analysis of variance showed that among treatments there is significant difference on growth parameters of tomato seedling (p < /em> 0.01), this indicate the positive impact of the bacterial endophyte on the growth parameters of tomato seedling is inoculated with the bacterial than the control plants. In this experiment, stem height, dry weight of stem, leaf and root, percentage of electrolyte leakage, chlorophyll a, chlorophyll b, carotenoid, proline and carbohydrate content were examined. The results of mean comparison showed that salinity stress significantly reduced stem height, stem dry weight, leaves and roots, chlorophyll a, chlorophyll b, carotenoids and increased electrolyte leakage; however, bacterial endophyte reduced the negative effects of salinity stress on tomatoes. Tomato seedling treated with endophytic bacteria showed higher levels of key osmolytes, total soluble carbohydrates and free proline compared to untreated plants under salinity stress. Conclusion: The results also showed that the use of endophytic bacteria increased the growth of tomatoes in saline soil and water, thereby it can be used as an effective tool for growing salinity-sensitive plants such as tomatoes in saline conditions.
M. Moradi; H.R. Roosta; A. Estaji
Abstract
Introduction: Iron is an essential element for plant growth which is involved in many plant processes such as photosynthesis and activating enzymes involved in mitochondrial and photosynthetic electron transfer. Iron (Fe) deficiency is a common disorder affecting plants in many areas of the world, and ...
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Introduction: Iron is an essential element for plant growth which is involved in many plant processes such as photosynthesis and activating enzymes involved in mitochondrial and photosynthetic electron transfer. Iron (Fe) deficiency is a common disorder affecting plants in many areas of the world, and is chiefly associated with high pH, calcareous soils. Plant Fe deficiency has economic significance, because crop quality and yields can be severely compromised. Deficiency or low activity of iron in the plant causes chlorophyll is not produced in sufficient quantities and the leaves are pale. The decrease of chlorophyll leading to the reduction of the plant food processor and finally the yield is reduced. Iron fertilizers are grouped into three main classes: inorganic Fe compounds (soluble ones such as FeSO4·7HO), synthetic Fe chelates [such as ethylenediamine tetraacetic acid (EDTA) and ethylenediamine-di (o-hydroxyphenylacetic acid) (EDDHA)] and natural Fe-complexes (humates and amino acids). Iron could be applied in different chemical forms, including chelates and inorganic Fe salts. To our knowledge, no published data for tomato growing under hydroponic conditions have assessed the effects of application methods and different sources of Fe Fertilizer on plant yields, growth and nutritional condition. Therefore, this work was carried out to study the effect of FeSO4, Fe-EDTA, Fe-EDDHA and Fe-DTPA as a foliar spray and root-applied on the growth, yield, physiological characteristics of tomato plants under hydroponic system. Material and Methods: Regarding to the role of application methods and Fe sources on the absorption of this element and the process of photosynthesis and plant growth, a factorial experiment was carried out to determine the best methods of application (add to nutrient solution and foliar spray) and iron fertilizer (FeSO4, Fe-EDTA, Fe-DTPA and Fe-EDDHA) for growth and physiological characteristics of tomato in hydroponic system with three replications. Analysis of variance (ANOVA) was performed using the SAS program. If ANOVA determined that the effects of the treatments were significant (P ≤0.01 for F-test), then the treatment means were separated by Tukey range test. Result and Discussion: The results indicated that the plant height, dry and fresh weight affected by the application methods and iron fertilizer, so that the maximum and minimum plant height, and dry and fresh weight were obtained in application of Fe-EDTA to nutrient solution and foliar application of FeSO4, respectively. Mohammadipour et al., (2013) reported that by applying nano-chelate fertilizer of iron, iron sulfate, Fe-EDTA and Fe-EDDHA by two methods of foliar application and root-applied (soil) of Spathiphyllum plant, a significant difference between the types of fertilizer and application method were used. So that the maximum height and dry weight of the plant were obtained in Fe-EDDHA fertilizer treatment to the root application. The root and leaf Fe concentration affected by source of iron and the method of application so Fe-EDTA added to nutrient solution and foliar application had the highest amount of Fe in the root and leaf, respectively. Cu, Mn, Zn and some macro elements such as; Mg and P also influenced by the type of fertilizer and method of application. Roosta and hamidpour (2013) showed that the foliar application of Fe-EDDHA under aquaponic and hydroponic conditions increased the amount of K, Mg, Fe and decreased the concentration of Zn, Cu and Mn compared to the control treatment in tomato plants. Current experiment results showed that the maximum chlorophyll content (a, b and total) and maximal quantum yield of PS II photochemistry (Fv/Fm) and performance index (Pi) values of young and old leaves were found with Fe-EDTA in nutrition solution and the highest carotenoids and sugar soluble content were found in FeSO4 in nutrient solution and foliar application, respectively. Roosta and Mohsenian (2012) reported that there was also a linear relationship between leaf-Fe and chlorophyll content in pepper. The application of inorganic Fe salt (FeSO4) and Fe-chelates (Fe-EDDHA and Fe-EDTA) on pepper plants increased Chl a content in leaves of these plants compared to the control. Additionally, several investigations have described the beneficial effects of foliar Fe. Iron deficiency caused a significant reduction in the amount of chlorophyll a, b, total and carotenoids of pea (Iturbe-Ormaetxe et l., 1995). Conclusion: Based on the results, Fe-EDTA and Fe-EDDHA had the highest significant effect on vegetative growth of tomato, respectively. Thus, at neutral pH of nutrient solution as occurred in this experiment, application of Fe-EDTA in nutrition solution is suitable than the other source of iron fertilizer for tomato growth.
M. Moradi; F. Razavi; V. Rabiei; M. Soleimani Aghdam; L. Salehi
Abstract
Introduction: In recent years, γ-aminobutyric acid (GABA), a non-proteinogenic four-carbon signaling amino acid, has been employed as a safe strategy for attenuating chilling injury and fungal decay, delaying senescence and keeping sensory and nutritional quality of fruits and vegetables during postharvest ...
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Introduction: In recent years, γ-aminobutyric acid (GABA), a non-proteinogenic four-carbon signaling amino acid, has been employed as a safe strategy for attenuating chilling injury and fungal decay, delaying senescence and keeping sensory and nutritional quality of fruits and vegetables during postharvest life. In addition to applying GABA as exogenous safe procedure, heightening cellular GABA shunt pathway activity also is pivotal for attenuating chilling injury and fungal decay, delaying senescence and keeping sensory and nutritional quality of fruits and vegetables during postharvest life. Low temperature storage is widely employed for prolonging postharvest life of fruits and vegetables accompanying by keeping sensory and nutritional quality. Tomato is one of the most important horticultural crops, which exhibits higher benefits for human health but being endemic to subtropical climates, they are very vulnerable to chilling injury. Cold storage application is normally employed as a regular low-cost real postharvest technology. Owing to its great socio-economic significance, great efforts have been done by researchers to attenuating chilling injury in tomato fruits during low temperature storage employing safe strategies such as melatonin, brassinosteroids, salicylic acid, nitric oxide, and gibberellic acid. Attenuating chilling injury in tomato fruits by postharvest treatments may attribute to keeping safe membrane integrity representing by lower electrolyte leakage and malondialdehyde (MDA) accumulation occurring by eliciting endogenous polyamines, proline and nitric oxide accumulation by activating CBF1 signaling pathway, hampering phospholipase D (PLD) and lipoxygenase (LOX) enzymes activity, activating reactive oxygen species (ROS) scavenging enzymes activity resulting in higher ascorbic acid and glutathione accumulation, maintaining endogenous GA3 homeostasis occurring by higher CBF1 signaling pathway concurrent with higher endogenous salicylic acid accumulation, which not only are pivotal for conferring chilling tolerance in tomato fruits but also are crucial for preserving sensory and nutritional quality.
Material and Methods: Tomato fruits (Solanum lycopersicum cv. Izmir) were picked at the mature green stage in Zanjan Province, Iran, and transported to the fruit analysis laboratory at Zanjan University. In the laboratory, the fruit was screened for uniform size, maturity, and absence of mechanical damage. Fruits (1440) were divided into four groups, each consisting of 360 fruits. The experiment was done in triplicate in which each replicate consisted of 120 fruits. The exogenous GABA applying was done by immersing of fruits in GABA at 0, 0.1, 1, and 5 mM for 15 min at 20 ˚C. Then, fruits were air dried at room temperature and stored at 4 ± 0.5 ºC (85–90 % RH) for 28 days. After assessment of chilling injury every 7 days during storage at 4 ˚C followed by shelf life at 25 ºC for 3 days, biochemical analyses were performed.
Results and Discussion: In recent experiment, we showed that the exogenous GABA applying, especially at 5 mM, is beneficial for attenuating chilling injury in tomato fruits during storage at 4 ºC for 28 days which was associated with higher membrane integrity representing by lower electrolyte leakage and malondialdehyde (MDA) accumulation. Keeping safe membrane integrity in tomato fruits in response to exogenous GABA applying may ascribe to triggering reactive oxygen species (ROS) scavenging catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) enzymes activity giving rise to higher endogenous ascorbic acid accumulation concomitant with promoting phenylpropanoid pathway activity representing by higher phenylalanine ammonia lyase (PAL) enzyme activity giving rise to higher phenols and flavonoids accumulation and superior DPPH scavenging capacity.
Conclusion: Therefore, exogenous application of GABA not only is proficient for attenuating chilling injury but also is beneficial for preserving nutritional quality of tomato fruits during storage at 4 ºC for 28 days.
B. Behzadi; A. Rahemi Karizaki
Abstract
Introduction: Tomato is a major source of the antioxidant, lycopene, which has been linked to many health benefits, including reduced risk of heart disease and cancer. It is also a great source of vitamin C, potassium, folate and vitamin K. Optimal planting dates and appropriate cultivars are farm management ...
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Introduction: Tomato is a major source of the antioxidant, lycopene, which has been linked to many health benefits, including reduced risk of heart disease and cancer. It is also a great source of vitamin C, potassium, folate and vitamin K. Optimal planting dates and appropriate cultivars are farm management cases that are effective in increasing yield. The purpose of the planting date is to find the planting time of a cultivar or a group of similar varieties of a plant so that the set of environmental factors at that time would be appropriate for the emergence, establishment and survival of the seedlings. Because of off-season production, tomato has a high economic status in Bushehr Province. Some researchers reported that cultivars with larger fruits had the lowest number of fruits per plant. By investigating the response of tomatoes to time of transplantation, fruit yield and crop characteristics of the cultivars, a number of researchers reported that the date of transplantation had a significant effect on the number of days to flowering, the number of branches per plant, the diameter of the fruit, the number of fruits per Plant and fruit yield, early transfer of seedlings increased the harvest period, while late planting date was significantly effective on flowering, and the plants sooner went to flower.
Materials and Methods: The study was conducted in order to determine the best transplanting date and tomato cultivars. An experiment was carried out in Randomized Completely Block Design with four levels of transplanting dates (6 Sep, 27 Sep, 17 Oct and 6 Nov) in main plots and four cultivars (Petopride 2, Peto Early CH, Cal J-N3 and Kimia) in sub plots with four replications in Bushehr Agricultural and Natural research resource center during 2009-2011.The spacing between rows of planting was 140 cm and plant spacing was 50 cm. Each plot included 4 planting lines of 4 meters long. Before planting in the summer, land preparation was done between June and August. The amount of fertilizer was determined based on soil test results. Phosphorus fertilizers, potassium, micronutrient and one third of nitrogen fertilizers and iron sequestrons, along with decayed animal manure, were mixed prior to deployment of type tubes and mixed with soil. One third of nitrogen fertilizers and iron sequestrons were given during flowering and one-third remained at the time of fruit start. Irrigation was carried out on a regular basis, taking into account the weather conditions and plant requirements. After each harvest, fruit yield, fruit number per plant, fruit weight, yield per plant were measured. To determine the length and diameter of fruit, acidity and soluble solids from each plot, 10 fruits were selected randomly.
Results and Discussion: Interaction effect among the tomato cultivars and transplanting dates on fruit yield, yield per plant, number of fruits per plant, fruit weight and fruit diameter were significant at 1% probability level. In the first year, the highest total yield and fruit yield per plant were obtained from the transplanting date of 6 September and Potoperide 2 cultivar, and the lowest yield was produced with November 15 transplanting date and Kimia cultivar. In the second year, the highest total yield and fruit yield per plant were obtained from the transplanting date of 27 September and kimia cultivar, and the lowest amount was obtained from the transplanting date of 6 November and Peto Early CH cultivar. Planting date is one of the most important management practices which affects the crop growth through the effect on photoperiod, day and night temperature, light intensity and soil moisture. In most cases, earlier planting dates due to the longer vegetative and reproductive growth period of the plant may improve the allocation of photosynthetic materials to the plants.
Conclusions: This study showed that fruit yield and yield components were significantly affected by planting date and cultivars. Generally, delay in planting reduces fruit yield, plant yield, fruit weight and number of fruits per plant. Environmental factors such as light, temperature, CO2, moisture content can be changed from year to year. These factors affect the number of flowers formed in the plant, their pollination, the percentage of fruit formation. Also, the potential of cultivars had a significant effect on yield and its components and the cultivars producing the shorter fruit had the least number of fruits per plant.
maryam haghighi; maryam mozafariyan
Abstract
Decreasing irrigation via partial root zoon drying (PRD) save water and decreased production expenses. For comparing common irrigation and PRD on tomato cv. Falcato an experiment was designed in Massey University of New Zealand. Treatments were control (irrigation in a Field capacity in each irrigation ...
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Decreasing irrigation via partial root zoon drying (PRD) save water and decreased production expenses. For comparing common irrigation and PRD on tomato cv. Falcato an experiment was designed in Massey University of New Zealand. Treatments were control (irrigation in a Field capacity in each irrigation to whole root zoon) and PRD (irrigation in a half of Field capacity in each irrigation to one side of root zoon). The results were shown that superoxide dismutase and peroxidase activity increased in PRD compare with control. Photosynthesis, mesophyll conductance, photosynthesis water efficiency, fresh and dry weight of root and shoot decreased in PRD. NACE (Nutrient Acquisition Efficiency) of leaf was the highest for Zn and Na and the lowest for P when PRD applied compare with control. Yield indices like MP increased significantly by 63% compare to first harvest. YSI (Yield Stability Index) increased by 46% in third harvest compare with second one. The TOL (Tolerance index) increased by 80% in last harvest compare with first one. GMP (Geometric Mean Productivity) in the second harvest increased by 62% compare to first one.
M. Haghighi; M. Mozafariyan; Z. Afifipour
Abstract
The superabsorbent polymer withholding water and disperse it gradually to roots increase soil water capacity. To study the effect of superabsorbent polymer on decreasing water stress effect as well as increasing irrigation efficiency a factorial experiment based on randomized block design with 4 replicates ...
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The superabsorbent polymer withholding water and disperse it gradually to roots increase soil water capacity. To study the effect of superabsorbent polymer on decreasing water stress effect as well as increasing irrigation efficiency a factorial experiment based on randomized block design with 4 replicates was design. Treatments were 0, 10 and 20 % V superabsorbent and irrigation levels 25, 50 and 100% field capacity in greenhouse of Shiraz University. The results were shown that drought stress decreased chlorophyll content, fresh and dry weight but did not effect on fruit parameters at 5%. Superabsorbent improved tomato growth in stress condition and in 50% FC,10% superabsorbent increased relative water content to 14%and fresh weight of root to 60%. Chlorophyll content increased to 22% in 25%FC with 20% superabsorbent relative water content and root fresh weight increased 28 and 53% respectively. In 25%FC with 20% superabsorbent. Superabsorbent elevated hazardous effect of drought stress and with holding the water in root zoon cues normal plant growth.
soheila kamari; Gh. Peyvast; M GH
Abstract
Humic acid, which has hormone- like activity , not only enhances plant growth and nutrients uptake but also improve stress tolerance. To determine the effect of humic acid on the yield and vegetative characteristics of tomato an experiment was conducted with tomato cv. `Isabela` in 2008 in a plastic ...
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Humic acid, which has hormone- like activity , not only enhances plant growth and nutrients uptake but also improve stress tolerance. To determine the effect of humic acid on the yield and vegetative characteristics of tomato an experiment was conducted with tomato cv. `Isabela` in 2008 in a plastic tunnel at the Agriculture Faculty, Guilan University using randomized completely design with four treatments (0, 1, 2, 3 mg l-1 humic acid) and three replications. Humic acid was sprayed four times during the vegetation period with 10- day intervals after three weeks of planting. Results showed that humic acid could affect significantly (P
M. Mahmoodnia; Mohammad farsi; S.H. Marashi; P. Ebadi
Abstract
Investigation of the cultivated tomato plant as a plant ideal system along with the drought resistant wild species can be useful to a better understanding of the mechanisms of drought resistance and improvement of tomato plants. To investigate the effect of drought stress on leaf Relative Water Content ...
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Investigation of the cultivated tomato plant as a plant ideal system along with the drought resistant wild species can be useful to a better understanding of the mechanisms of drought resistance and improvement of tomato plants. To investigate the effect of drought stress on leaf Relative Water Content (RWC), electrolyte leakage and photosynthetic parameters in four species of tomato (a cultivated species and three wild species) at two levels of irrigation (field capacity and 40% field capacity) and four time periods (before stress, 10 days after stress, 20 days after stress and recovery) a factorial experiment based on Completely Randomized Design (CRD) was used. Among the tested species, cultivated species showed the highest decrease in RWC. Electrolyte leakage was significantly increased in stress conditions. The rate of photosynthesis and chlorophyll fluorescence yield in the two drought resistant wild species increased in stress conditions unlike of cultivated species, probably because of more adaptation of these species with low water conditions. The results showed that each of these species according to their own mechanism for dealing with drought stress, in one or more properties are superior to other species. it is therefore suggested that the different species for future studies (molecular) with different characteristics to be used.
