In order to evaluate the effect of substrate and enrichment material on biological efficiency (BE) and other physical and chemical propertiese of medicinal mushroom shiitake (Lentinula edodes) an investigation was perfomed as factorial experiment in completely randomized designe with four replications in Khorramabad city of lorestan continent Iran. Experimental factors were substrate in three levels, 1- chikpea straw 2- sawdust 3- wheat straw, and enrichment material in four levels, 1- sugarcane bagasse 2- sugarbeet molasses 3- rice brane and 4- control (without enrichment). Result indicated that BE of mushroom for substrate was 72% and 48% higher than chickpea and wheat straw respectively. Rice bran enrichment had the significant effects on BE and was increased BE by 56% incomparision to control treatment. Enrichment treatment of sugarcane bagasse and sugarbeet molasses had not significant effect on mushroom BE. Among the substrates sawdust with 57.7% had the highest carbohydrate levels in shiitake mushroom. The highest level of carbohydrate (63.3%) was belonged to rice bran enrichment that had significant differences whit control and two others enrichments. Substrate had not significant effect on mushroom lipid. The highest level of mushroom lipid (3.3%) was attributed to rice bran enrichment. Rice bran enrichment was increased mushroom lipid by 65, 57 and 50% incomparision to control, sugarbeet molasses and sugarcane bagasse respectively