Journal of Horticultural Science
with the collaboration of Iranian Scientific Association for Landscape (ISAL)

Comparative Study of Pulse and Permanent Treatments of Nanosilver, Salicylic Acid and Some Essential Oils on Gerbera (Gerbera jamesonii Bol.) ‘Rosalin’ Traits

Document Type : Research Article

Authors

M. S. Motaghayer
M. Azizi
A. Tehranifar

- Department of Horticulture Science and Landscape Architecture, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.22067/jhs.2021.60101.0
Abstract
Introduction
Gerbera (Gerbera jamesonii Bolus, Asteraceae) commonly known as Transvaal Daisy, is one of the ten most popular and important commercial cut flowers. Gerbera is a perennial, tropical, herbaceous plant with colorful and attractive flowers that are widely used as a decorative garden plant or cut flowers. The most important problem of the gerbera cut flowers is short vase life. The end of vase life of cut gerbera flowers is often due to bending of the scape. The aim of this study was to screen the effects of nanosilver and salicylic acid as pulse treatment and sucrose and thyme, clove and peppermint essential oils as permanent treatment on vase life and some physiological and biochemical traits of gerbera ‘Rosalin’ cut flowers.

Materials and Methods
This study was conducted as a factorial experiment based on completely randomized block design with three replications. The first factor was pulse treatments using nanosilver (NS) 5 and 10 mg.L-1 (Nanocid Company, Iran), salicylic acid (SA) 50 and 100 mg.L-1 (Merck Company) and distilled water as control, and the second factor was permanent treatments applying distilled water, sucrose 4% (Merck Company), peppermint (100 mg.L-1), thyme (100 mg.L-1) and clove (300 mg.L-1) essential oils (EO) (Zardband Company, Iran). Pulse treatments were applied for 24 h. Treated stems were then stood into vases containing permanent treatments. Vase solutions were freshly prepared at the beginning of the experiment and not renewed during of the study. The measured traits were: flower vase life, flower water content (WC), relative fresh weight (RFW), water loss (WL), water uptake (WU), total soluble solid (TSS), superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) amount (Biovision Colorimetric Assay Kit, USA). The experiment was conducted in the laboratory at 20-22°C, 40-50% RH, and 15 µmol.m-2.s-1 light intensity (cool white florescent tubes) under a daily light period of 12 hours. The obtained data were analyzed using MSTAT-C program and mean comparison was done using LSD range test.

Results and Discussion
The results showed that NS 10 mg.L-1 with thyme EO 100 mg.L-1 (14.25 days) and NS 10 mg.L-1 followed by peppermint EO 100 mg.L-1 (14 days) had the best effect on longevity and maintaining the water content of gerbera cv. ‘Rosalin’ cut flower, compared to other treatments. Flower WC was high (about 90%) except in 4% sucrose permanent treatment flowers which had negative effect and decreased more rapidly during the vase life period. Despite antimicrobial preservative application, RFW, WU, WL and TSS generally had a decreasing trend during the experiment. However, it was observed that flowers treated by NS 10 mg.L-1 (0.86 g.g-1), thyme (0.77 g.g-1) and peppermint (0.72 g.g-1) EOs had the highest RFW on the eighth day of the experiment, but 4% sucrose (0.59 g.g-1) permanent treatment reduced RFW during cut flower vase life. The highest WU was observed in SA 100 mg/L (67.28 g), NS 5 (61.31 g) and 10 mg/L (60.35 g) on the second day. According to the results of this research, pulsing by NS 5 and 10 mg.L-1 and SA 100 mg.L-1 resulted in the most WL on day two (64.24, 57.85 and 64.95 g respectively). TSS of the cut flowers decreased with time, however reduction rates were delayed in SA and NS treated flowers. On the eighth day of experiment, long-term treatment showed the greatest effect on TSS amount. Flowers treated by NS 10 mg.L-1 followed by 4% sucrose and peppermint EO treatment had the highest TSS amount, respectively 3.50 and 3.17 Bx. As time passed, the activities of SOD enzyme increased, however, treated flowers showed significantly higher SOD activity compared to the control. Especially NS 10 mg.L-1 pulse treatment, followed by thyme and peppermint EOs, indicated the highest amounts (241.43% and 233.94%) of SOD activity on the seventh day of the experiment. During the experiment, MDA increased in the flowers, so that the highest amount of MDA was detected in the control (67.30%) on the seventh day and the lowest was produced in NS 10 mg/L treated flowers followed by thyme (14.88%) and peppermint (19.44%) EOs. Due to the fact that these treatments had the best effects on flower vase life, these results are logical.

Conclusion
Based on the results of this study, new antimicrobial agents such as NS, thyme and peppermint EOs had a positive effect on flower vase life, WC, RFW and WU and reduced the rate of WL and TSS reduction in flowers. It might be due to this fact that these are very effective antimicrobial agents, inhibited the microbial growth and prevented bacterial plugging in conducting tissues. NS particles enter to cell, tissue and organs, so they can inhibit the respiration and electron transfer system and material transfer in microbial cell membrane. The application of natural phenolic compound like EOs reduced the accumulation of MDA and enhanced SOD enzyme activity. Therefore, due to the undesirable results of chemical preservative on human and environmental health, EOs can be very good substitutes for preserving cut flowers postharvest life.

Keywords

Cut flower
Nanoparticles
Peppermint essential oil
Thyme essential oil
Vase life
Subjects

Authors retain the copyright. This is an open access article distributed under Creative Commons Attribution 4.0 International License (CC BY 4.0).

 

1.            Abdel-Kader, H., & Rogers, M. N. (1985). Postharvest treatment of Gerbera jamesonii. In III International Symposium on Postharvest Physiology of Ornamentals, 181, 169-176. https://doi.org/10.17660/ActaHortic.1986.181.20
2.            Aghajani, N., & Jafarpour, M. (2016). Effects of pre-and postharvest treatments of silicon and rice hull ash on vase life of gerbera. International Journal of Horticultural Science and Technology, 3(1), 77-87. https://doi.org/10.22059/ijhst.2016.58164
3.            Alaey, M., Babalar, M., Naderi, R., & Kafi, M. (2011). Effect of pre- and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase life of rose cut flowers. Postharvest Biology and Technology, 61(1), 91-94. https://doi.org/10.1016/j.postharvbio.2011.02.002
4.            Alt, V., Becher, T., Steinrucke, P., Wagener, M., Seidel, P., Dingeldein, E., Domann, E., & Schnettler, R. (2004). An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials, 25(18), 4383-4391. https://doi.org/10.1016/j.biomaterials.2003.10.078
5.            Amini, S., Jafarpour, M., & Asgari, K. (2014). Effect of temporary and permanent treatments of extracts of thyme and stevia on postharvest quality of gerbera cut flowers. Australian Journal of Basic and Applied Sciences, 8(8), 93-98.
6.            An, C., & Mou, Z. (2011). Salicylic acid and its function in plant immunity. Journal of Integrative Plant Biology, 53(6), 412-428. https://doi.org/10.1111/j.1744-7909.2011.01043.x
7.            Ansari, S., Hadavi, E., Salehi, M., & Moradi, P. (2011). Application of microorganisms compared with nanoparticles of silver, humic acid and gibberellic acid on vase life of cut gerbera Good Timing. Journal of Ornamental and Horticultural Plants, 1(1), 27-33.
8.            Arora, J. S., & Singh, K. (2002). Pre and post-harvest management of cut flowers. Indian Horticulture, 46: 20-23.
9.            Babarabie, M., Zarei, H. & Varasteh, F. (2016). Potential of increasing the vase life and improvement of some physiological characteristics of alstroemeria cut flowers by using non-harmful compounds environmentally. Journal of Chemical Health Risks, 6(1), 1-8.
10.        Balestra, G. M., Agostini, R., Bellincontro, A., Mencarelli, F., & Varvaro, L. (2005). Bacterial populations related to gerbera (Gerbera jamesonii L.) stem break. Phytopathologia Mediterraneae, 44(3), 291-299. https://doi.org/10.14601/Phytopathol_Mediterr-1798
11.        Bhanusree, M. R., Rao, N. H., Chandrika, M., Vinayakumari, M., Kumar, K. R., Shukla, G., & Chakravarty, S. 2015. Effect of sucrose on biochemical parameters of cut gerbera flowers (Gerbera jamesonii Bolus ex. Hook.) cv. Lamborgini. Journal of Agriculture and Technology, 2(1 & 2), 68-71.
12.        Borochov, A., & Woodson, R. (1989). Physiology and biochemistry of flower petal senescence. Horticultural Review, 11, 15-43. https://doi.org/10.1002/9781118060841.ch2
13.        Boukaew, S., Prasertsan, P., & Sattayasamitsathit, S. (2017). Evaluation of antifungal activity of essential oils against aflatoxigenic Aspergillus flavus and their allelopathic activity from fumigation to protect maize seeds during storage. Industrial Crops and Products, 97, 558-566. https://doi.org/10.1016/j.indcrop.2017.01.005
14.        Bradley, D. G., & Min, D. B. (1992). Singlet oxygen oxidation of foods. Critical Reviews in Food Science and Nutrition, 31(3), 211-236. https://doi.org/10.1080/10408399209527570
15.        Burt, S. (2004). Essential oils: Their antimicrobial properties and potential applications in foods: A review. International Journal of Food Microbiology, 94(3), 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
16.        Chang, H., Siegel, B. Z., & Siegel, S. M. (1984). Salinity-induced changes in isoperoxidases in taro Colocasia esculenta. Phytochemistry, 23(2), 233-235. https://doi.org/10.1016/S0031-9422(00)80308-8
17.        Conner, D. E. (1993). Naturally occurring compounds. p. 441-468. In: P. M. Davidson & A. L. Branen (Eds.) Antimicrobials in Foods. Marcel Dekker, New York.
18.        Da Silva, J. A. T. (2003). The cut flower: postharvest considerations. Journal of Biological Sciences, 3(4), 406-442. https://doi.org/10.3923/jbs.2003.406.442
19.        Danaee, E., Naderi, R., Kalatejari, S., & Ladan Moghadam, A. R. (2013). Evaluation the effect of nanosilver with salicylic acid and benzyladenine on longevity of gerbera flowers. Journal of Basic and Applied Scientific Research, 3(8), 682-690.
20.        Dareini, H., Abdos, V., & Danaee, E. (2014). Effect of some essential oils on postharvest quality and vase life of gerbera cut flowers (Gerbera jamesonii cv. Sorbet). European Journal of Experimental Biology, 4(3), 276-280.
21.        Ding, C. K., Wang, C. Y., & Gross, K. C. (2002). Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta, 214(6), 895–901. https://doi.org/10.1007/s00425-001-0698-9
22.        Dole, J. M., & Wilkins, H. F. (2006). Floriculture: Principles & Species (Second edition). NJ: Pearson/Prentice Hall. Upper Saddle River, U.S.A. 1023 p.
23.        Dorman, H. J. D., Peltoketo, A., Hiltunen, R., & Tikkanen, M. J. (2003). Characterization of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food chemistry, 83(2), 255-262. https://doi.org/10.1016/S0308-8146(03)00088-8
24.        Ferrante, A., Alberici, A., Antonacci, S., & Serra, G. (2007). Effect of promoter and inhibitors of phenylalanine ammonia-lyase enzyme on stem bending cut gerbera flowers. International Conference on Quality Management in Supply Chains of Ornamentals, 755, 471-476. https://doi.org/10.17660/ActaHortic.2007.755.64
25.        Furno, F., Morley, K. S., Wong, B., Sharp, B. L., Arnold, P. L., Howdle, S. M., Bayston, R., Brown, P. D., Winship. P. D., & Reid, H. J. (2004). Silver nanoparticles and polymeric medical devices: A new approach to prevention of infection. Journal of Antimicrobial Chemotherapy, 54(6), 1019-1024. https://doi.org/10.1093/jac/dkh478
26.        Geng, X. M., Liu, J., Lu, J. G., Hu, F. R., & Okubo, H. (2009). Effects of cold storage and different pulsing treatments on postharvest quality of cut OT Lily ‘Mantissa’ flowers. Journal of the Faculty of Agriculture, Kyushu University, 54, 41-45. https://doi.org/10.5109/14035
27.        Ghaderi, G. M., Mamashloo, S., Sadeghi, M. A., & Alami, M. (2011). Evaluation of antioxidant activity, reducing power and free radical scavenging of different extract of Artemisia annua L. Journal on Plant Science Researches, 21, 46-57. (in Persian)
28.        Hayat, Q., Hayat, S., Irfan, M., & Ahmad, A. (2010). Effect of exogenous salicylic acid under changing environment: A review. Environmental and Experimental Botany, 68(1), 14-25. https://doi.org/10.1016/j.envexpbot.2009.08.005
29.        He, S., Joyce, D. C., Irving, D. E., & Faragher, J. D. (2006). Stem end blockage in cut Grevillea ‘Crimson Yu-lo’ inflorescences. Postharvest Biology and Technology, 41(1), 78-84. https://doi.org/10.1016/j.postharvbio.2006.03.002
30.        Hoseini, S. P., & Korehpaz, S. (2015). Effect of peppermint essential oil and salicylic acid on quality and vase life of cut tuberose flowers (Polianthes tuberosa cv. Pearl). In: Proceedings of Dubai 2nd International Conference on “Engineering and Technology, Computer, Basic and Applied Sciences”. 18-19 December, Dubai, UAE.
31.        Ichimura, K., Kojima, K., & Goto, R. (1999). Effect of temperature, 8-hydroxyquinoline sulphate and sucrose on the vase life of cut rose flowers. Postharvest Biology and Technology, 15(1), 33-40. https://doi.org/10.1016/S0925-5214(98)00063-5
32.        In, B. C., Chang, M. K., Byoun, H. J., & Son, K. C. (2010). Effect of vase water temperature and leaf number on water relations and senescence of cut roses. Korean Journal of Horticultural Science and Technology, 28(4), 609-617.
33.        Jalili Marandi, R., Hassani, A., Abdollahi, A., & Hanafi, S. (2011). Improvement of the vase life of cut gladiolus flowers by essential oils, salicylic acid and silver thiosulfate. Journal of Medicinal Plants Research, 5(20), 5039-5043.
34.        Jamshidi, M., Hadavi, E., & Naderi, R. (2012). Effects of salicylic acid and malic acid on vase life and bacterial and yeast populations of preservative solution in cut gerbera flowers. International Journal of AgriScience, 2(8), 671-674.
35.        Jiang, H., Manolache, S., Wong, A. C. L., & Denes, F. S. (2004). Plasma-enhanced deposition of silver nanoparticles onto polymer and metal surfaces for the generation of antimicrobial characteristics. Journal of Applied Polymer Science, 93(3), 1411-1422. https://doi.org/10.1002/app.20561
36.        Jowkar, M. M., & Salehi, H. (2005). Effects of different preservative solutions on the vase life of cut tuberose flowers at usual home conditions. In VIII International Symposium on Postharvest Physiology of Ornamental Plants, 669, 411-415. https://doi.org/10.17660/ActaHortic.2005.669.54
37.        Kalemba, D. A. A., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10(10), 813-829. https://doi.org/10.2174/0929867033457719
38.        Kaltaler, R. E. L., & Steponkus, P. L. (1976). Factors affecting respiration in cut roses. Journal of American Society for Horticultural Science, 101, 352-354. https://doi.org/10.21273/JASHS.101.4.352?urlappend=%3Futm_source%3Dresearchgate.net%26utm_medium%3Darticle
39.        Kazem Alvandi, R., Sharifan, A., & Aghazadeh Meshghi, M. (2011). Study of chemical composition and antimicrobial activity of peppermint essential oil. Journal of Comparative Pathobiology Iran, 353-363. (In Persian).
40.        Kazemi, S., Hassanpour Asil, M., & Ghasemnezhad, M. (2014). Physiological effects of some essential oils in comparison with 8-hydroxyquinoline in cut lisianthus flowers (Eustoma grandiflorum L.). Iranian Journal of Horticultural Science, 45(2), 185-195. (in Persian). https://doi.org/10.22059/ijhs.2014.51960
41.        Kilic, T., & Cetin, E. S. (2014). Determination of the effects of sage and balm extracts on vase life in gerbera cv. Rosalin. Tarum Bilimleri Arasturma Dergisi, 7(2), 13-15.
42.        Knee, M. (2000). Selection of biocides for use in floral preservatives. Postharvest Biology and Technology, 18(3), 227-234. https://doi.org/10.1016/S0925-5214(99)00074-5
43.        Liu, J. P., He, S. G., Zhang, Z. Q., Cao, J. P., Lv, P. T., He, S. D., Cheng, G. P., & Joyce, D. C. (2009). Nanosilver pulse treatments inhibit stem end bacteria on cut gerbera cv. Ruikou flowers. Postharvest Biology and Technology 54, 59-62. https://doi.org/10.1016/j.postharvbio.2009.05.004
44.        Loubaud, M., & Van Doorn, W. G. (2004). Wound-induced and bacteria-induced xylem blockage in roses, Astilbe and Viburnum. Postharvest Biology and Technology, 32(3), 281–288. https://doi.org/10.1016/j.postharvbio.2003.12.004
45.        Mates, J. M., Perez-Gomez, C., & De Castro, I.,N. (1999). Antioxidant enzymes and human diseases. Clinical biochemistry, 32(8), 595-603. https://doi.org/10.1016/S0009-9120(99)00075-2
46.        Mehdikhah, M., Onsinejad, R., Ilkaee, M. N., & Kaviani, B. (2016). Effect of salicylic acid, citric acid and ascorbic acid on post-harvest quality and vase life of gerbera (Gerbera jamesonii) cut flowers. Journal of Ornamental Plants, 6(3), 181-191.
47.        Mortazavi, N., Naderi, R., Khalighi, A., Babalar, M., & Allizadeh, H. (2007). The effect of cytokinin and calcium on cut flower quality in rose (Rosa hybrida L.) cv. Illona. Journal of Food Agriculture and Environment, 5(3&4), 311-313.
48.        Motaghayer, M. S., & Esna-Ashari, M. (2009). Effect of different concentrations of four preservative solutions on tuberose (Polianthes tuberosa L.) cut flower vase-life. Floriculture and Ornamental Biotechnology, 3(1), 59-61.
49.        Nair, S. A., Singh, V., & Sharma, T. V. (2003). Effect of chemical preservatives on enhancing vase-life of gerbera flowers. Journal of Tropical Agriculture, 41, 56-58.
50.        Nazari Deljou, M. J., Pour Youssef, M., Karamian, R., & Jaberian Hamedani, H. (2012). Effect of cultivar on water relations and postharvest quality of gerbera (Gerbera jamesonii Bolus ex. Hook f.) cut flower. World Applied Science Journal, 18(5), 698-703.
51.        Nikolić, M., Glamočlija, J., Ferreira, I. C., Calhelha, R. C., Fernandes, Â., Marković, T., Marković, D., Giweli, A., & Soković, M. (2014)a. Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Industrial Crops and Products, 52, 183-190. https://doi.org/10.1016/j.indcrop.2013.10.006
52.        Nikolić, M., Jovanović, K. K., Marković, T., Marković, D., Gligorijević, N., Radulović, S., & Soković, M. (2014)b. Chemical composition, antimicrobial, and cytotoxic properties of five Lamiaceae essential oils. Industrial Crops and Products, 61, 225-232. https://doi.org/10.1016/j.indcrop.2014.07.011
53.        Nowak, J., & Rudnicki, R. M. (1990). Postharvest handling and storage of cut flowers, florist greens and potted plants. Timber Press, Oregon, U.S.A. 210 p.
54.        Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety, 60(3), 324-349. https://doi.org/10.1016/j.ecoenv.2004.06.010
55.        Paull, J., & Lyons, K. (2008). Nanotechnology: The next challenge for organics. Journal of Organic Systems, 3(1), 3-22.
56.        Perik, R. R., Razé, D., Harkema, H., Zhong, Y., & Van Doorn, W. G. (2012). Bending in cut Gerbera jamesonii flowers relates to adverse water relations and lack of stem sclerenchyma development, not to expansion of the stem central cavity or stem elongation. Postharvest Biology and Technology, 74, 11-18. https://doi.org/10.1016/j.postharvbio.2012.06.009
57.        Prashanth, P., Sekhar, R. C., & Reddy, K. C. S. (2010). Influence of floral preservatives on scape bending, biochemical changes and postharvest vase life of cut gerbera (Gerbera jamesonii Bolus ex. Hook.). Asian Journal of Horticulture, 5(1), 1-6.
58.        Rai, M., Yadav, A., & Gade, A. (2009). Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27(1), 76-83. https://doi.org/10.1016/j.biotechadv.2008.09.002
59.        Reddy, B. S., & Singh, K. (1996). Effects of aluminium sulphate and sucrose on vase life of tuberose. Journal-Maharashta Agricultural Universities, 21, 201-203.
60.        Rios-Gonzalez, K., Erdei, L., & Lips, S. H. (2002). The activity of antioxidant enzymes in maize and sunflower seedlings as affected by salinity and different nitrogen sources. Plant Science, 162(6), 923-930. https://doi.org/10.1016/S0168-9452(02)00040-7
61.        Saghazadeh, F. Khodadadi, M., & Mobasser, H. R. (2014). Effects of different concentrations of plant chemicals on vase life of rose varieties Utopia. International Journal of Farming and Allied Sciences, 3(2), 152-154.
62.        Solgi, M., Kafi, M., Taghavi, T. S., & Naderi, R. (2009). Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv. Dune) flowers. Postharvest Biology and Technology, 53, 155-158. https://doi.org/10.1016/j.postharvbio.2009.04.003
63.        Taiz, L., & Zeiger, E. (2002). Plant Physiology. Sinauer Associates, Sunderland, Massachusetts. U.S.A. 690 P.
64.        Tehranifar, A., Vahdati Mashhadian, N., Selahvarzi, Y., & Bayat, H. (2013). Treatment with salicylic acid extends the vase life of important commercial cut flowers. Advanced Crop Science, 3(6), 405-413.
65.        Turkan, I., Bor, M., Ozdemir, F., & Koca, H. (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168(1), 223-231. https://doi.org/10.1016/j.plantsci.2004.07.032
66.        Vahdati Mashhadian, N., Tehranifar, A., Bayat, H., & Selahvarzi, Y. (2012). Salicylic and citric acid treatments improve the vase life of cut chrysanthemum flowers. Journal of Agricultural Science and Technology, 14(4), 879-887. https://dor.isc.ac/dor/20.1001.1.16807073.2012.14.4.8.4
67.        Van Doorn, W. G. (2001). Role of soluble carbohydrates in flower senescence: A survey. Acta Horticulturae, 543, 179-183. https://doi.org/10.17660/ActaHortic.2001.543.21
68.        Van Ieperen, W., Van Meeteren, U., & Nijsse, J. (2002). Embolism repair in cut flower stems: A physical approach. Postharvest Biology and Technology, 25(1), 1-14. https://doi.org/10.1016/S0925-5214(01)00161-2
69.        Van Meeteren, U. (1978.) Water relations and keeping quality of cut gerbera flowers. I. The cause of stem break. Scientia Horticulturae, 8(1), 65-74. https://doi.org/10.1016/0304-4238(78)90071-7
70.        Van Son, N. (2007). Response of gerbera (Gerbera jamesonii Bolus) varieties to micro-propagation. Master of Science Thesis in Horticulture (Agriculture). University of Agricultural Sciences, Dharwad, India.
71.        Ziyaei Movahed, Z., Kafi, M., Khalighi, A., Azizi, M., & Sharifi, R. (2010). Investigation of the possibility in replacing natural ingredients (essential oil and extracts of Australian Cheesewood) instead of antibacterial chemicals ingredients in preservative solution of the Gerbera cut flower. Iranian Journal of Horticultural Science, 41, 337-345 (In Persian).
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Journal of Horticultural Science
Volume 40, Issue 2
Summer 2026
Pages 236-217

  • Receive Date 24 August 2020
  • Revise Date 15 April 2021
  • Accept Date 07 March 2022
  • First Publish Date 07 March 2022

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