Research Article
Medicinal Plants
Atefeh Bakherad; Majid Azizi
Abstract
Introduction: the capacity to sense and respond to light is widespread in animals, plants, fungi and bacteria. In nature, light is one of most crucial environmental signals for developmental and physiological processes in various organisms, including filamentous fungi. The ability to sense light is crucial ...
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Introduction: the capacity to sense and respond to light is widespread in animals, plants, fungi and bacteria. In nature, light is one of most crucial environmental signals for developmental and physiological processes in various organisms, including filamentous fungi. The ability to sense light is crucial for many organisms determining timing and modality of development and orienting primary and secondary metabolic processes. Due to the increased number of sequenced fungal genomes, numerous genes encoding proteins involved in light sensing and downstream gene regulation have been identified. This expedited understanding the mechanisms by which the fungus activates physiological and morphological reactions in response to light. One of the One of these fungi is the Monascus purpureus. the genus Monascus was classified in the family Monascaceae, but based on recent genome sequencing, it seems that the genus is more closely associated with the genus Aspergillus, and thus should be reclassified in the Aspergillaceae family. The fungus Monascus is the most famous for production of pigments and monacolins; however, it can also form other metabolites – citrinin, dimerumic acid, and GABA (γ-aminobutyric acid). Formation of particular metabolites depends on the strain and cultivation conditions. Monascus pigments (MPs) as natural food colorants have been widely utilized in food industries in the world, especially in China and Japan. Moreover, MPs possess a range of biological activities, such as anti-mutagenic and anticancer properties, antimicrobial activities, potential anti-obesity activities, and so on. So, in the past two decades, more and more attention has been paid to MPs. In this study, the effect of different LED light spectra on growth and secondary metabolites of Monascus purpureus is studied.Material and methods: In this study, in order to investigate the effect of light quality on growth rate, biomass and pigment production in Monascus purpureus, this fungus was cultivated under dark conditions and three light, white, red and blue LEDs and harvested in three times (seventh, tenth and fourteenth days) To measure the amount of pigment in the liquid medium. To measure the radial growth rate and biomass weight, a solid medium was used. Also, the absorbance of the samples was measured in 410, 470, and 510 nm wavelengths for yellow, orange and red pigments,respectively, using a spectrophotometric device.Results: The results of this study shows that there is a significant correlation between pigment and optical spectra, so that the highest amount of pigment (yellow, orange, red) was obtained in terms of darkness, with values of 0.344, 0.291, 0.249, respectively. There was no significant difference between harvest time and pigment content except for red color on day 10 in dark conditions, with a value of 0.249. The smallest amount of pigment is also related to the red pigment produced on the 14th day in the blue light spectrum with an absorption of 0.009. Also, the highest radial growth rate was observed in the dark condition with a mean growth of 5.12 mm / day and the lowest radial growth was related to the cultivation of monascus under the blue light spectrum with an average growth of 2.61 mm / day. However, there was no significant difference between biomass and light in solid media. The highest amount of biomass in the liquid medium was 0.126 g in 40 ml of culture medium with monascus culture in dark conditions on day 14 and the lowest amount of biomass was also obtained with 0.068 g in 40 ml of culture medium in white light on the seventh day.Concolusion: The results showed that, in addition to factors such as carbon source, nitrogen source, PH, temperature, minerals, partial pressure of oxygen, other microorganisms, light is also an important environmental factor for development and physiological processes in Monascus purpureus. According to this study, the growth of this fungus in the dark causes increased cell growth, the amount of biomass and pigment, these results indicate that the lack of light stimulates the growth and production of secondary metabolites in this fungus. Although there was no significant difference between pigment value and harvest time, except for the red pigment, but due to the slight difference in pigmentation on the 10th day of cultivation in dark conditions, it can be deduced that the best conditions for producing the highest amount of pigment, cultivation of Monascus fungus under conditions The darkness and harvest of this mushroom is on the tenth day of cultivation. Also, among pigments produced by this fungus, the amount of yellow pigment production in different light conditions is higher than other pigments. Chen, M. H. & Johns, M. R. (1993) shows that the solid-state yielded greater amount of pigment9,8 compared to submerged fermentation. This phenomenon had been attributed to the pigment because of low solubility in the fermentation medium and pigment accumulation within the mycelium in submerged culture10. In contrast, in solid-state fermentation, the Monascus sp penetrated into solid substrate and pigment released onto the surface. Solid state not only issued nutrient, but also acted as anchorage for cells, this may have bestowed to the celebrated eminent productivity. Solid state environment is similar to environment of fungi which was normally excited. Therefore, it is suggested that in subsequent studies, to achieve maximum pigment, a solid culture medium should be used.
Research Article
Medicinal Plants
Ghasem Esmaeili; Majid Azizi; Hossein Arouiee; Jamil Vaezi
Abstract
Introduction: The genus Salvia L. (Lamiaceae) is one of the most interesting plant genera with a variety of annual, biennial, perennial and shrub forms. According to recent reports, there are 61 species of Salvia genus in Iran, among which 18 species are endemic. The use of Salvia species dates back ...
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Introduction: The genus Salvia L. (Lamiaceae) is one of the most interesting plant genera with a variety of annual, biennial, perennial and shrub forms. According to recent reports, there are 61 species of Salvia genus in Iran, among which 18 species are endemic. The use of Salvia species dates back to ancient times, and they are now being used for different purposes such as antimicrobial, anthelmintic, anti-inflammatory, tonic, carminative, digestive, Alzheimer’s disease, antispasmodic as well as for traditional medicines and food industries. Compared to crop plants, the breeding programs of medicinal plants are still in nascent stages due to high biological and biochemical variations. Since breeding programs, especially in medicinal plants, are expensive, suitable mating can help reduce the costs of breeding programs. Understanding the flowering biology, reproductive systems and pollination mechanism is essential to management and protection of ecosystems and mating programs. Despite the high diversity of Salvia genus in Iran, there is a paucity of studies on reproductive biology.Materials and methods: seeds of seven Salvia species including S. virgata (with four accessions), S. frigida, S. nemorosa, S. atropatana, S. macrosiphon, S. sclarea, and S. syriaca were collected from different geographical regions. The seeds were treated by various methods such as washing, cold treatment (stratification), scarification (sand and sulfuric acid), Gibberellic acid (GA3) and KNO3 to enhance germination. The seedlings were produced at the greenhouse and after growing as high as 15 cm or reaching the10-leaf stage, the seedlings were transferred to the farm. Plants phenology and flowering duration were recorded in the second year. Furthermore, some flower characteristics and pollinator were monitored. The Various pollination treatments were designed to investigate the breeding system of Salvia species including open pollination, spontaneous autogamy, geitonogamy, xenogamy, apomixis, and control. The self-incompatibility index (ISI) was calculated for each species in different pollination treatment.Results and discussion: The germination study exhibited that washing seeds for 24 h and applying 250 ppm GA3 exerted the highest effect on seed germination. The seedlings in early growth stages required medium to high porosity (high permeability to water and air) like natural habitat, but they were sensitive to dry and high EC at these stages. The phenology studies were shown Salvia virgata (all accessions) and S. nemorosa flowered three months after cultivation and other species flowered in the second year. The results showed that all species had long flowering periods ranging from 15 (S. atropatana) to 41 days (S. virgata). Increased flowering period led to the enhancement of flower over-lapping and the sharing of the pollen among different plants or species. Two species including S. atropatana and S. syriaca had heterostyly flowers (long-styled morph). The staminal-lever-mechanism was observed in all species. The results of manual pollination indicated that open pollination provided the best treatment with the highest average of seed set (73.63%) in all species. Salvia virgata (A1) with a seed set of 90.3% was the best species, and S. syriaca with a seed set of 51.5% demonstrated the poorest performance in open pollination system. The seed set in the xenogamy treatment of different species and accessions was in the range of 10.2 (S. syriaca) to 32.5% (S. frigida). It was observed that seed set among different species in geitonogamy and spontaneous autogamy varied widely from 2.60 - 17.30% and 2.98 - 12.18%, respectively. In treatments with a wide variety of pollen sources, the fruit set was higher, indicating that the pollen limitation triggered low seed set.Conclusion: Salvia species in the present study have a high potential for cultivation as medicinal and ornamental due to suitable adaptability and long flowering period. Salvia species were relatively self-incompatible that intention to the out-crossing and breeders can use in breeding programs as a valuable characteristic. The self-incompatible and specific structure in flower and pollination are the reasons for the diversity of this taxa.
Research Article
Medicinal Plants
ELAHE GHAFOOREAN HOZORI ISFAHAN; Majid Azizi
Abstract
IntroductionMedicinal plant are very importnat in health care for today societies and the need for it is increasing. Greenhouse production of transplants face different limitations including light. LED lights are introduced for solving the problem. An LED lamp or LED light bulb is ...
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IntroductionMedicinal plant are very importnat in health care for today societies and the need for it is increasing. Greenhouse production of transplants face different limitations including light. LED lights are introduced for solving the problem. An LED lamp or LED light bulb is an electric light for use in light fixtures that produces light using one or more light-emitting diodes (LEDs). LED lamps have a lifespan many times longer than equivalent incandescent lamps, and are significantly more efficient than most fluorescent lamps. Experiments revealed surprising performance and production of vegetables and ornamental plants under LED light sources. Many plant species have been assessed in greenhouse trials to make sure that the quality of biomass and biochemical ingredients of such plants is at least comparable with those grown in field conditions. Plant performance of mint, basil, lentil, lettuce, cabbage, parsley and carrot was measured by assessing both the health and vigor of the plants and the success of the LEDs in promoting growth. Also noticed was profuse flowering of select ornamentals including primula, marigold and stock.Materials and Methods In order to investigate the effect of supplementary LED light on the growth parameters and the amount of active ingredient in Hypericum perforatum L. “Topaz” cultivar, a research was conducted in two independent experiments. The research was set up in a completely randomized design with three replications. The first experiment experienced the extended of day length with 4 hours at the end of the day (started from sunrise to sunset). LED treatments were started at 10 cm height of the transplants. The second experiment examined the light types. In this order the considered treatments consisted of four levels of red light (L1), blue light (L2), combination of 67% red light and 33% blue light (L3), white (L4), 100% natural light(L5), with an intensity equal to (200 fc). Measurement of traits in the first experiment was carried out at flowering stage. Aerial part fresh and dry weights, number of black nodes, days to flowering, leaf area, total chlorophyll, antioxidant activity, total phenol, plant height and hypericine percentage were determined within both experiments.Results and Discussion The results showed that the simple effect of supplementary combination red and blue light (started from sunrise to sunset) on growth, morphological, physiological and biochemical traits was significant at 1% probability level. The highest plant dry weight was detected in L3 in the first and second harvests respectively (8.63 g, 66.29 g). Also, the highest percentage of Hypericine in the first harvest was obtained for L3 (0.17%). Measurement of the traits in the second experiment was carried out at the seedling stage. Results showed that the simple effect of supplementary combination light (sun exposure and day length increased for 4 hours) had a significant effect (p≤0.01) on growth rate, physiological, morphological and biochemical properties. So that the lowest number of days to flowering (early flowering) was obtained from L3 treatment (101 day), and the highest percentage of Hypericin was related to L3 treatment (0.156%). Black node numbers and total phenol content were increased by 27 and 53 % with supplementary light treatments respectively. Antioxidant activity was also raised and enhanced from 33 % to 75 % with LED light application. Plant height and total chlorophyll content were enhanced from 13 to 23 cm and 12 to 22 mg/g FW as well. Regarding studied traits and the obtained results of the both experiments, the positive effect of applying LED light, red and blue on the growth and biochemical properties, especially the Hypericine percentage and yield of St. John’s wort is quite evident.Different reactions were observed in response to supplementary light treatments for St. John’s wort. Most of the enhancements observed were mainly due to optimized photosynthesis activities and more metabolic production processes.Conclusions Regarding studied traits and the obtained results of the both experiments, the positive effect of applying LED light, red and blue on the growth and biochemical properties, especially the Hypericine percentage and yield of St. John’s wort is quite evident. It seems that a combination of red and blue light could be suggested for solving the light limitations and increasing the medicinal plant production for future.Keywords: Supplementry light, light quality, Hypericum perforatum, Hypericine
Research Article
Medicinal Plants
mahdieh kheirabadi; Majid Azizi
Abstract
Introduction: The allelopathic potential of saffron in three experiments were investigated.Materials and Methods: In this study, we examined the aqueous and aqueous methanol extract of saffron (Crocus sativus L.), 9-year-saffron cultivated soils and non-saffron cultivated soils. Activated carbon and ...
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Introduction: The allelopathic potential of saffron in three experiments were investigated.Materials and Methods: In this study, we examined the aqueous and aqueous methanol extract of saffron (Crocus sativus L.), 9-year-saffron cultivated soils and non-saffron cultivated soils. Activated carbon and Zeolite were used to ameliorate allelopathy activity.lettuce (Lactuca sativa L.) was used as a test plant and the percentage of germination, hypocotyl length, radicle length and their ratio was evaluated This study was carried out in a completely randomized design with four replications in the Laboratory of Horticultural Sciences, Faculty of Agriculture, the Ferdowsi University of Mashhad in 2018- 2019.Results and Discussion: The results of this study showed that the allelopathic effect of aqueous and methanol extract of the saffron corm was more effective on germination and seedlings growth of lettuce than 9-year-saffron cultivated soils. The use of activated carbon and zeolite adsorbents was effective in improving and mitigating the allelopathy activity and increased the growth of lettuce seedlings.Conclusions: the results of this study indicated that it is possible to inhibit allelopathic activity in saffron fields and this study should be repeated in field conditions in order to recommend a practical solution for this purpose.
Research Article
Medicinal Plants
Somayeh Sadeghifard; Majid Azizi; Sara Karimi
Abstract
Introduction: Growth regulators play a vital role in developmental stages of plants and their use can be improved the yield of crops. One of the most important compounds that used as growth regulators in recent years is Salicylic acid. The application of salicylic acid in the activation of systemic acquired ...
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Introduction: Growth regulators play a vital role in developmental stages of plants and their use can be improved the yield of crops. One of the most important compounds that used as growth regulators in recent years is Salicylic acid. The application of salicylic acid in the activation of systemic acquired resistance, and metabolite synthesis and antioxidant enzymes has been proved. Also, salicylic acid as a natural compound has potential to prevent ethylene production and its effect. In order to evaluate the effect of salicylic acid in different concentration on yield and oils content of Ziziphora, a split plot experiment with three replications during 2014-2015 in the farm of Agricultural Faculty of Shirvan and Ferdowsi University of Mashhad laboratories was conducted.Material and Methods:This research was conducted during 2014-2015 in field conditions in a split plot design in time,at Shirvan Agricultural Research Station(latitude º40 / 37, longitude º93 / 57 and with a height of 1097 m above sea level) and in labrovatory Department of Horticultural Sciences, Ferdowsi University of Mashhad. Each plot had an area of 4 square meters in furrow planting way which the distance between rows were 50 cm and on rows 15 cm and were carried out on 25 March 2014. IMediately after planting, irrigation was done, and after 20 days which the seeds germinated as well, second irrigation was done. Irrigation and weed controls also be done manually once a week. Salicylic acid (Sigma Aldrich, 99.5%) in the three phases of plant growth (vegetative growth, during vegetative growth and flowering time), at concentrations (0, 10-2, 10-4 and 10-6 M) in three repeats were sprayed. The first spraying (the growth)on 3 April 2014, the second spraying (during growth) on 4 May and the third one (at the time of flowering) was conducted in early June 2014. Plant harvesting was done on 20 July. In order to measure the dry weight of plants, the samples were dried for a week in shade condition. Essential oil content was determined using 30 grams dry sample in 500 ml of water by hydrodistillation using Clevenger for 4. For biochemical characteristics first samples extract were obtained. To prepare extracts, dry samples were milled then one gram of each sample was transferred to the 50 ml Erlen and 10 ml of 80%methanol was added and shaked for 24 hours. After 24 hours the extract was filtered using filter paper then used for measuring phenols, flavonoids and antioxidant activity. Phenol measurment: phenolic content was measured using the Folin reagent. In 5.0 mL of each extract (10 milligrams per ml) 5.2 ml Folin reagent was added. After 5 minutes, 300 ml of a molar solution of sodium carbonate is added and for 30 minutes in a bath that was 40 °C. The sample absorbance at 760 nm was measured by a spectrophotometer against the blank. Gallic acid was used as the standard for calibration curve. Flavonoids measurement: Flavonoid content of the samples were measured using aluminum chloride reagent. In 5.0 ml of methanol extract (10 milligrams per ml), 5.1 ml of methanol, 1.0 ml of 10% aluminum chloride, 1.0 ml of a molar potassium acetate and 8.2 ml of distilled water was added. The mixture kept in dark for 30 minutes and absorbance at a wavelength of 415 nm was read against the blank. Quercetin was used as the standard for calibration curve evaluation. JMP statistical software was used to analyze the data.Results and Discussion: Based on the results application of salicylic acid affect fresh and dry weight, plant height, phenol and flavonoid content significantly ( P
Research Article
Medicinal Plants
Majid Azizi; Somayye Beigi; Zeynab safaei; Meysam Mansouri
Abstract
IntroductionMedicinal plants are economical important plants that are used in traditional medicine and industry as raw or processed materials. Valerian (Valeriana officinalis L.) is one of the important medicinal plants which belong to Valerianaceae family. The valerian rhizome and roots have been considered ...
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IntroductionMedicinal plants are economical important plants that are used in traditional medicine and industry as raw or processed materials. Valerian (Valeriana officinalis L.) is one of the important medicinal plants which belong to Valerianaceae family. The valerian rhizome and roots have been considered as a valuable medicinal plant that essential oil content (between 0.1 to 2 percent) varied according to climatic conditions of production location. Valerian needs a lot of water during the growth period and enough moisture around the roots and rhizome increase root yield. Iran is located in arid and semi-arid region. If the least water requirement of plant does not provide, plant face to drought stress and irreparable damage is imported to the product. Nowadays, the use of superabsorbent polymers is one of the ways to increase irrigation efficiency. They are made of hydrocarbons, can store high water or aqueous solutions in root zone of plants and to reduce negative effects of drought stress. So, improvement of plant growth, increasing of irrigation intervals, reducing water loss and costs of irrigation are due to application of superabsorbent polymers. Generally, water efficiency, dry matter production and root development, are positive reactions to the use of superabsorbent. In this regard, Stockosorb® copolymer is potassium-based nutrients that have a high ability to absorb water and nutrients and high strength polymer maintained the water has been proven. The aims of this investigation were to study the effects of Stockosorb® hydrophilic polymers, on some morphological (root dry weight and yield, leaf area), biochemical (chlorophyll a, b), physiological (electrolyte leakage, leaf relative water content) characteristics and essential oil content and yield of valerian under drought stress.Materials and MethodsThis research was conducted in field (1×1 m2) at Department of Horticultural Science‚ College of Agriculture‚ Ferdowsi University of Mashhad. The research was set out in a factorial experiment on the basis of completely randomized block design. The Stockosorb® hydrophilic polymer at four concentrations (0, 100, 200, 300 gr/m2) and two irrigation period (6 and 10 day) with three replications were set as treatments and leaf area, root dry weight and yield, chlorophyll a, b, electrolyte leakage, leaf relative water content and essential oil content and yield were evaluated at the end of the growth period. The seeds of Valeriana officinalis were sown in protected open field for seedling production and the seedling were transplanted to the field at four-leaf stage (12 plant per plot). Stockosorb® mixed with soil, after weighing the polymers based on determined concentration. After seedling establishment, all plots were irrigated with a determined amount and equal of water.Results and DiscussionThe results showed, using Stockosorb® superabsorbent polymer be useful for water supply the plant in water stress condition. Application of this substance on some morphological, biochemical and physiological characteristics and valerian essential oil, was significant at 1% and superior to the control. According to the results obtained, Stockosorb® 300gr/m2 with irrigation period 6 day interval, increased leaf area, root dry yield, leaf relative water content, chlorophyll a and essential oil yield. While, the highest valerian root dry weight and essential oil content were obtained with application of Stockosorb® 200gr/m2 and irrigation period of 6 days interval. The highest electrolyte leakage was related to control and irrigation period 10 days interval and the maximum chlorophyll b was belonged to Stockosorb® 100 gr/m2 with irrigation period 10 days interval.ConclusionSince final goal in cultivation of valerian as other essential oil bearing plants is providing the best conditions for increasing the yield and quantity of essential oil at the same time, according to the results of this study superabsorbent polymer in higher concentrations and less irrigation period, controlled the drought stress and improved all measured characteristics. The highest root dry weight under drought stress was related to more accessible and preservatives water in the root zone and it found by using the middle concentrations (200 gr/m2) super absorbent polymer and irrigation period shorter (6 day). Using of mentioned treatments, according to the middle water stress, leads to increased valerian essential oil. The best root dry yield and essential oil yield, with sufficient amounts of water and better growth conditions were achieved by application of 300 gr/m2 Stockosorb® and irrigation period 6 days interval. In general, superabsorbent polymer was able to increase irrigation period and this was evident in qualitative and quantitative results related to the valerian in the treatment of 300 gr/m2 with irrigation period 10 day and 100 gr/m2 of polymer 6 day irrigation period.
Research Article
Medicinal Plants
Monireh Abdollahpoor; Majid Azizi; Siamak Kalantari; Yusef Ali Saadat
Abstract
Introduction Hypericum perforatum L. is an important medicinal plant that used for depression treatment. In vitro regeneration has been successfully achieved for many Hypericum species from a range of explants sources with different growth regulators. Recent studies have demonstrated that in vitro culture ...
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Introduction Hypericum perforatum L. is an important medicinal plant that used for depression treatment. In vitro regeneration has been successfully achieved for many Hypericum species from a range of explants sources with different growth regulators. Recent studies have demonstrated that in vitro culture is an option for multiplication of different Hypericum species. With consideration this notice that tissue culture can provide an affordable alternative method for propagation with high speed to production of intensive plant material as well as suitable materials for breeding programs of H. perforatum, the objective of this study was to investigate the effects of explant types (leaf and stem), plant growth regulators (2,4-Dichlorophenoxyacetic acid (2,4-D), Naphthalene Acetic Acid (NAA), Benzyl Adenine (BA) and Kinetin (Kin)), light (dark and weak light), media culture (MS medium and MS medium with B5 vitamins) and poly vinyl pyrrolidone (PVP) on callus induction in H. perforatum at in vitro culture condition. Materials and Methods For preparation of sterile plantlets, the seeds of Iranian H. perforatum (Azadshahr population) were cultured in 1.2 MS growth regulator free-media. For investigation the callus induction of Iranian H. perforatum the leaf and stem explants of in vitro obtained plantlets were used. Explants were cultured in different concentrations of 2,4-D (0.2, 0.5 and 1 mg l-1) with two kinds of cytokines BA and Kin (0.2, 0.5 and 1 mg l-1) as well as 1 mg l-1 NAA. For browning control of calli the effects of light (dark and weak light), media culture (MS medium and MS medium with B5 vitamins) and four concentrations of poly vinyl pyrrolidone (0, 50, 100 and 200 mg l-1) were also surveyed. Results and Discussion Callus cultures could be used for cell suspension initiation, studying of their morphogenetic potential and screening of secondary metabolite profile. In present study the response of two H. perforatum explants (leaf and stem) to different levels and combinations of auxins and cytokinins were tested. The callus induction in both studied explants (leaf and stem) was just observed in supplemented media with plant growth regulators. The calli of leaf explants were showed better growth in dark and the highest callus fresh weight was obtained in 0.25 mg l-1 2,4-D + 1 mg l-1 Kin and 0.5 mg l-1 2.4-D + 1 mg l-1 BA. Of the various concentrations and combination of growth regulators, the minimum response about callus induction was observed in the presence of 1 mg l-1 2, 4-D in combination with 1 mg l-1 NAA. The obtained calli got browning shortly after induction. The investigation of light effect on callus quantity and quality showed that not only light did not affect the callus induction and callus browning but also reduced the callus growth. The highest callus fresh weight was obtained in 100 mg l-1 poly vinyl pyrrolidone in leaf explant. Few species within the genus Hypericum have been used to produce callus. In H. perforatum seedling, different explants such as shoot apical meristem, stem segments and leaves were used for callus induction. In H. erectum callus induction was obtained by culturing seedlings in the presence of Indole Acetic Acid (IAA) and BA under darkness. The combination of cytokinins and auxins did not support callus growth of H. brasiliense and callus of nodal explants was only obtained in the presence of 2,4-D or NAA using either MS or B5 medium. These differences among literatures can be due to different cultivars, culture conditions, explant type and medium composition.Conclusion The plant growth regulators is necessary for callus induction in Iranian H. perforatum and leaf is suitable for this purpose. The light intensity and poly vinyl pyrrolidone did not control the browning of of H. perforatum calli.
Research Article
Postharvest physiology
Mahroo Sadat Motaghayer; Majid Azizi; Ali Tehranifar
Abstract
Introduction: Gerbera (Gerbera jamesonii Bolus, Asteraceae) commonly known as Transvaal Daisy, is one of the ten most popular and important commercial cut flowers. Gerbera is a perennial, tropical, herbaceous plant with colorful and attractive flowers that are widely used as a decorative garden plant ...
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Introduction: Gerbera (Gerbera jamesonii Bolus, Asteraceae) commonly known as Transvaal Daisy, is one of the ten most popular and important commercial cut flowers. Gerbera is a perennial, tropical, herbaceous plant with colorful and attractive flowers that are widely used as a decorative garden plant or cut flowers. The most important problem of the gerbera cut flowers is short vase life. The end of vase life of cut gerbera flowers is often due to bending of the scape. The aim of this study was to screen the effects of nanosilver and salicylic acid as pulse treatment and sucrose and thyme, clove and peppermint essential oils as permanent treatment on vase life and some physiological and biochemical traits of gerbera ‘Rosalin’ cut flowers.Materials and Methods: This study was conducted as a factorial experiment based on completely randomized design with three replications. The first factor was pulse treatments using nanosilver (NS) 5 and 10 mg/L (Nanocid Company, Iran), salicylic acid (SA) 50 and 100 mg/L (Merck Company) and distilled water as control, and the second factor was permanent treatments applying distilled water, sucrose 4% (Merck Company), peppermint (100 mg/L), thyme (100 mg/L) and clove (300 mg/L) essential oils (EO) (Zardband Company, Iran). Pulse treatments were applied for 24 h. Treated stems were then stood into vases containing permanent treatments. Vase solutions were freshly prepared at the beginning of the experiment and not renewed during of the study. The measured traits were: flower vase life, flower water content (WC), relative fresh weight (RFW), water loss (WL), water uptake (WU), total soluble solid (TSS), superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) amount (Biovision Colorimetric Assay Kit, USA). The experiment was conducted in the laboratory at 20-22°C, 40-50% RH, and 15 µmol/m2s light intensity (cool white florescent tubes) under a daily light period of 12 hours. The obtained data were analyzed using MSTAT-C program and mean comparison was done using LSD range test.Results and Discussion: The results showed that NS 10 mg/L with thyme EO 100 mg/L (14.25 days) and NS 10 mg/L followed by peppermint EO 100 mg/L (14 days) had the best effect on longevity and maintaining the water content of gerbera cv. ‘Rosalin’ cut flower, compare to other treatments. Flower WC was high (about 90%) except in 4% sucrose permanent treatment flowers which had negative effect and decreased more rapidly during the vase life period. Despite of antimicrobial preservative application, RFW, WU, WL and TSS generally had a decreasing trend during the experiment. However, it was observed that flowers treated by NS 10 mg/L (0.86 g/g) and thyme (0.77 g/g) and peppermint (0.72 g/g) EOs had the most RFW on the eighth day of experiment, but 4% sucrose (0.59 g/g) permanent treatment reduced RFW during cut flower vase life. The highest WU was respectively observed in SA 100 mg/L (67.28 g), NS 5 (61.31 g) and 10 mg/L (60.35 g) on the second day. According to the results of this research, pulsing by NS 5 and 10 mg/L and SA 100 mg/L resulted in the the most WL on day two (64.24, 57.85 and 64.95 g respectively). TSS of the cut flowers decreased with time, however reduction rates were delayed in SA and NS treated flowers. On the eighth day of experiment, long-term treatment showed the most effect on TSS amount. Flowers treated by NS 10 mg/L followed by 4% sucrose and peppermint EO treatment had the highest TSS amount, respectively 3.50 and 3.17 Bx. As the time passed, the activities of SOD enzyme increased, however, treated flowers showed significantly higher SOD activity compared to the control. Especially NS 10 mg/L pulse treatment, followed by thyme and peppermint EOs, indicated by the highest amounts (241.43% and 233.94%) of SOD activity on seveth day of the experiment. During the experiment, MDA increased in the flowers, so that the highest amount of MDA was detected in control (67.30%) on the seventh day and the lowest was produced in NS 10 mg/L treated flowers followed by thyme (14.88%) and peppermint (19.44%) EOs. Due to the fact that these treatments had the best effects on flower vase life, these results are logical.Conclusions: Based on the results of this study, new antimicrobial agents such as NS, thyme and peppermint EOs had a positive effect on flower vase life, WC, RFW and WU and reduced the rate of WL and TSS reduction in flowers. It might be due to this fact that these are very effective antimicrobial agents, inhibited the microbial growth and prevented bacterial plugging in conducting tissues. NS particles enter to cell, tissue and organs, so they can inhibit the respiration and electron transfer system and material transfer in microbial cell membrane. The application of natural phenolic compound like EOs reduced the accumulation of MDA and enhanced SOD enzyme activity. Therefore, due to the undesirable results of chemical preservative on human and environmental health, EOs can be very good substitutes for preserving cut flowers postharvest life.
Research Article
Medicinal Plants
Negin Afsharzadeh; Majid Azizi; Leila Samiei
Abstract
Introduction Hypericum perforatum is a perennial plant that has been used in traditional medicine. H. perforatum have several types of medicinal compounds including antiviral compounds, antioxidants, flavonoides and also has valuable compounds such as Hypericin, Hyperforin, Pseudo-hypericin and xanthones ...
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Introduction Hypericum perforatum is a perennial plant that has been used in traditional medicine. H. perforatum have several types of medicinal compounds including antiviral compounds, antioxidants, flavonoides and also has valuable compounds such as Hypericin, Hyperforin, Pseudo-hypericin and xanthones that have effect on human physiology. Aerial parts of H. perforatum are dotted with dark glands that appear as black or red nodules. Black glands were known as localization of secondary metabolites. as a consequence of the commercial potential of this species attempts have been at plant improvement by application of in vitro culture methods. Among seedling explants of H. perforatum, it appears that roots are superior for shoot regeneration. It is generally accepted that explants source is an important factor r successful establishment of tissue culture in many cases. Production of secondary metabolites via plant cell and tissue culture yields various advantage es, including standardization and quality. These criteria are also valid for the main economically important chemical in st. John’s wort, namely hypericin, pseudo hypericin and hyperforin. The aim of this study was to evaluate the effectof some tissue cultures on plant Callogenesis, regeneration and also, study the effect of cytokinin and auxin on rooting rate and shoot multiplication. Materials and Methods: This research included two experiments; first experiment plan was a completely randomized in the form of a factorial. Second experiment plan was completely ranom.first part of experiment : this part were conducted with two explants, leaf and shoot ,maintained in light and dark condition. Shoot explants were derived from sterile seedlings that was obtained from seeds were cultured on MS medium. Seeds were decontaminated by NaClO 20% (V/V) for 20 min and were washed with sterile deionized water. Leaf explants were derived from seedlings in the in vivo condition and decontaminated by NaClO 20% (V/V) for 20 min then washed with sterile deionized water. Both of explants cultured on MS media supplemented with BA(3 and 4 mg/l) and 2,4-D (0, 1 and 2.5 mg/l). Callogenesis and regeneration was measured after 4 weeks.Second part of experiment: shoot of indirect regeneration for rooting study, were cultured on ½ MS media supplemented with BA (0 and 5/0 mg/l) and IBA (0 and 1 mg/l). Proliferation, shoot and root length were measured after 4 and 8 weeks.Results and Discussion:Effects of the factors on first part of experiment; calluses of shoot and leaf explants were induced after 4 weeks. Shoot explants Medium supplemented with 4 mg/l BA and 2.5 mg/l 2, 4-D showed 95% Callogenesis. Leaf explants Medium supplemented with 3 mg/l BA and 2.5 mg/l 2, 4-D showed 98% Callogenesis. Shoot explants Medium supplemented with BA 3 mg/l showed 62% regeneration and leaf explants Medium supplemented with BA 4 mg/l showed 8% regeneration.For second part of experiments; root induction on half strength medium without hormone and medium supplemented with 0.5 mg/l BA and 1 mg/l IBA had highest rooting frequencies. Average of root length was registered 5.25 cm. half strength medium supplemented with 0.5 mg/l BA had 100% and also, medium with 0.5 mg/l BA and 1 mg/l IBA had 86% shoot multiplication and were not appeared any roots. Average Shoot length on medium without hormone and medium supplemented with 0.5 mg/l BA and 1 mg/l IBA was registered 6.24 cm and in media with 0.5 mg/l BA and 1 mg/l IBA was registered 1.8 cm. based on result of this experiments, the concentration levels of the two hormone BA and 2, 4-D in the induction of calli formation and regeneration of the H. perforatum have been effective. In the second experiment, hormone BA, in the absence of IBA did not cause rooting and increased the degree of shoots and ultimately proliferation was effective. Also, in treatment with 0.5 mg/l BA and 1 mg/l IBA, although the concentration of cytokinin was less than auxin, rooting was not done. conclusionThe goal of this study was to introduce the suitable medium for Callogenesis and regeneration of H. perforatum for production and breeding aims. Tissue and cell culture methods are used for various purposes. The result of each section can be used effectively in research topics, including corrective tests, processes for increasing secondary metabolites, as well as increasing commercial products. Therefore, by introducing efficient and effective methods, it is possible to increase the production of plants by micropropagation in less time.
Research Article
Growing vegetables
Ladan Ajdanian; Hossein Arouiee; Majid Azizi
Abstract
Introduction: As an artificial light source, LED bulbs can help to grow better and faster greenhouse products. Meanwhile, blue and red light are important for plant growth. The quality of light in terms of color and wavelength can affect the morphological structure of the plants. Therefore, the use of ...
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Introduction: As an artificial light source, LED bulbs can help to grow better and faster greenhouse products. Meanwhile, blue and red light are important for plant growth. The quality of light in terms of color and wavelength can affect the morphological structure of the plants. Therefore, the use of additional lights in winter, when light exposure is less than normal, can improve plant growth and flowering. It is also advisable to change the quality of light by using artificial light sources in controlled environments such as greenhouses in order to change the quantity and quality of agricultural products, especially the fruits of the fruit.Materials and Methods: In this research, the effect of complementary light and nutrition (commercial Calfomyth solution containing high calcium phosphorus and calcium elements along with micronutrient elements) as a pot experiment under non-soil culture conditions in the greenhouse, in the form of split-based plots experiment A completely randomized design with 3 light treatments including: natural light (control), 60% red light + 40% light blue and 90% red light + 10% blue light, and 2 nutritional treatments including: non-spraying with commercial fertilizer, Calfomyth and spraying A concentration of 2,000 in 3 replicates was established and implemented. The traits that were studied in this experiment are: 1- leaf number (count of leaves), 2- plant height by meter, with accuracy 0.01 m, 3- diameter with caliber with accuracy 0.01 mm, 4- Number of flowers (counting), 5- Time of flowering until the product reaches (counting the number of days), 6- Performance: Whole and red ripe fruits were planted and weighed separately from the total fruit weight Per plant was obtained in grams per plant. Statistical analysis was performed using JMP8 software and comparison of meanings was done using LSD test at 1% and 5% probability level.Results and Discussion: The results showed that the application of additional light on fruit yield, stem diameter and plant height, yield time and leaf number (p≤0.05) were significant. Spraying with Calfomyth could have a significant effect on stem diameter and number of leaves. In the traits such as fruit yield, number of flowers and leaves, the interaction effect of feeding and supplementary light was also significant. The highest number of leaves belonged to 60% red + 40% blue treatment, as well as spraying with Calfomyth (118), the highest number of flowers (50) was related to 90% red + 10% blue and Calfomyth treatment, and the smallest number ( 15) was related to control treatment. Also, the highest yield (3553 g of fruit per plant) was obtained in Calfomyth treatment with 90% red + 10% blue treatment and the lowest yield (434 g fruit per plant) The control was light and non-foliar treatment. Although light is an important source of photosynthesis, photosynthesis can also be related to a series of optical regulators and optical sensors. Blue and red light cause different optical sensors and expression of genes, each of which can have a positive or negative effect on plant growth and development. Therefore, it can be concluded that the presence of both wavelengths (blue and red) is necessary for conducting, and for this reason, more research is now focused on achieving an appropriate optical composition.Conclusions: In this research, all of the morphological traits examined in the tomato plant were subjected to additional exposure by LED bulbs, each of which had a special effect on their receptors in the plant. Maximize growth and yield in the plant. As expected, blue light on vegetative traits and red light were more effective on reproductive traits. It can be said that the existence of both wavelengths (blue and red) is essential for the better and complete growth of the plant. In addition to the positive effect of wavelengths, the positive effect of leaf spraying can also be observed, which, along with the neodymium, could improve growth. The results of this study showed that the performance and growth under the cover of LED light (red and blue combination) were superior to natural conditions. Therefore, it could be suggested that the use of these lamps, as well as spraying with Calfomyth commercial fertilizer, could be feasible for better production in controlled conditions (greenhouse).It seems that application of complementary lights with proper nutrition can improve the performance and growth of tomato plants in greenhouse conditions.
Research Article
Medicinal Plants
Fatemehzahra Amirmohammadi; Majid Azizi; Seyyed Hossein Nemati
Abstract
IntroductionThe Nepeta is one of the largest genus of Nepetoide subfamily, in the Lamiaceae family with about 79 species in Iran. Most of the species are perennial rarely annual with different vegetative forms, leaves covered with trichome. Glandular trichomes are widely distributed all over the aerial ...
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IntroductionThe Nepeta is one of the largest genus of Nepetoide subfamily, in the Lamiaceae family with about 79 species in Iran. Most of the species are perennial rarely annual with different vegetative forms, leaves covered with trichome. Glandular trichomes are widely distributed all over the aerial part of Nepeta species, and their largely to its great importance and their structures can vary widely among plants species. Some species contain monoterpenes, sesquiterpenes, cyclopentanoid iridoids derivatives and nepetalactones which they are used in folk medicine as, diaphoretic, diuretic, antitussive, febrifuge agent. In the present work, scanning electron microscopy (SEM) was used to determine the nutlet micromorphology and also micromorphology and distribution of the glandular trichomes of Nepeta binaludensis “an Iranian endemic species” and Nepeta cataria in order to improve the knowledge of the species and to evaluate the usefulness of this feature for phytochemical and systematic purpose. The chemical composition of its essential oil was also analyzed. This paper provides a detailed description of trichome morphology and nutlet in Nepeta binaludensis andNepeta catariaspecies growing in Iran, by scanning electron microscopy (SEM), and evaluates the systematic significance of such characteristics. Materials and MethodsThe research was conducted in research garden of Agricultural Faculty of Ferdowsi University of Mashhad during 2017. Seeds of two Nepeta species were provided by Institute of Plant Sciences in Ferdowsi University of Mashhad.The seeds were cultivation in greenhouse and the seedling (10 cm long) were transplanted in the field. During the summer aerial parts of the plants were harvested at flowering stage, dried in shade, and (30 g) were hydro distilled for 3 h using Clevenger apparatus. For SEM study, leaves were fixed with 3% glutaraldehyde in 0.1 M sodium phosphate buffer, pH 7.2 for 30 min at 4 °C. After washing in water the material was dehydrated through an ethanol gradient and critical point dried. Specimens were mounted onto SEM stubs using double-sided adhesive tape and coated with palladium. For nutlets observation, seed were directly mounted onto aluminum stubs using double-sided adhesive tape and coated with palladium and photographed. ResultBased on the obtained data, the surface leaves of both species (N. binaludensis and N.cataria) was observed forglandular and non-glandular trichomes distribution. (The glandular trichomes of the peltate types). Trichome density and size was variable as 7-21mm2 leaf surface (63-77 µm) in N. binaludensis and as 20-30 mm2 leaf surface (47-67 µm) in N.cataria. In both species only one types of glandular trichome was identified. Peltate trichomes comprise of a basal cell situated in the leaf epiderm with a four-celled secretory head. Nutlets of Nepeta were blackish-brown or brown in color. One type surface ornamentation, can be distinguished: smooth-type. The nutlet ornamental pattern was smooth type with finely granulated in N. binaludensis and reticulate pattern in N.cataria species. Water-distilled essential oil of the aerial parts of two speciescultivated in field, were analyzed by GC and GC/MS. In essential oil of N.binaludensis and N. cataria, 51 and 26 components were identified, respectively. The main components of the essential oil of N. binaludensis were 1.8-cineole (14.8%), β-Pinene (10.4%) and p-Cymene (9.8%). Main compounds of the essential oil of N. cataria were 4a-a, 7-a, 7a-b-Nepetalactone (72/76%) and 4a-a, 7-a,7a-a-Nepetalactone (17.86%). Depending on the composition of key compound identified in essential oils, Nepeta species have been divided in two groups. Conclusions Depending on the composition of main compound identified in the essential oils, Nepeta species have been divided in two groups. On the basis of our research N. binaludensis belong to 1.8-cineole chemotype and of N.cataria belong to the nepetalactone chemotype. The leaves of N. binaludensis, N.cataria have be glandular and non-glandular trichomes. One types of glandular trichomes are identified: Peltate trichomes.Trichomes are distributed at the surface of leaves, having various functions and are extremely variable in the plants, plant organs location, density, form, therefore their morphology and structure could be of taxonomical importance in plant. Glandular trichomes contain or secrete substances which are widely used in pharmaceutical and cosmetic as well as in pesticide industry. The variation in the structure, morphology, density, secretion and function is noticed among these trichomes distributed on different plant organs. Nutlet surface can be useful as a taxonomic characteres.
Research Article
Medicinal Plants
Zhale Zandavifard; Majid Azizi; Majid Darroudi; Azar Hosseini
Abstract
Introduction:Ganoderma lucidum is a high medicinal value mushroom have been widely used in the Far East countries especially in traditional Chinese medicine as promoting human health and treatment of many diseases. Nowadays, many published studies have established it contains a high source of nutraceutical ...
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Introduction:Ganoderma lucidum is a high medicinal value mushroom have been widely used in the Far East countries especially in traditional Chinese medicine as promoting human health and treatment of many diseases. Nowadays, many published studies have established it contains a high source of nutraceutical and pharmaceutical substances with potent and unique properties as immune suppressors, hypercholesterolemic agents, or coadjutant treatments in diseases such as cancer, hypertension, insomnia, anorexia, dizziness, and chronic hepatitis, among others. This species is rich in several bioactive compounds (over 400 compounds) mainly, including polysaccharides, triterpenoids, steroids, fatty acids, amino acids, nucleotides, proteins, and alkaloids. Herein, the fruiting bodies of G. lucidum were studied in terms of nutritional value and chemical composition analysis. and further assessment of antioxidant activity of extracts from the fruiting body. Materials and methods: In order to detection of nutrient elements, the samples were homogenized by microwave digestion (Milestone Ethos, Germany) with 1000 W maximum power and further characterized using Inductively coupled plasma optical emission spectroscopy (ICP-OES). Biochemical molecule contents were characterized using Acquity Ultra-Performance Liquid Chromatograph (UPLC, Waters) coupled to a photodiode array detector (PDA, Waters) and an electrospray ionization quadrupole time-of-flight tandem mass spectrometer (ESI–QTOF/MS; Waters). In order to assess antioxidant activity, two kinds of extract including methanol 80 % (ME) and hot water (HWE) as solvent were prepared by ultrasonic method. Six different in vitro assays are used for the determination of antioxidant capacity including ABTS, DPPH, superoxide (SO), nitric oxide (NO) free radicals scavenging, iron-reducing power (FRAP), and iron chelating activity (ICA). The data were analyzed by one-way analysis of variance (ANOVA) and the means were separated by the Newman-Keuls Multiple Comparison test (GraphPad Prism 8, San Diego, CA, USA)). All data were expressed as mean ± standard deviation. P ≤ 0.05 values or less were considered to indicate a statistically significant difference. Furthermore, Half-maximum inhibitory concentration (IC50) values for each assay were calculated from linear or logarithmic regression using Excel software.Results and Discussion: G.lucidum was characterized in terms of nutritional value and chemical composition. Generally, to study the nutraceutical value of G.lucidum, 14 elements were analyzed by ICP-OES. Amongst the macronutrient group, phosphorus and potassium (2910.8 and 1510.8 mg/kg dry matter) and in the micronutrient iron and zinc (8.5 and 7.74 mg/kg dry matter) have the highest amounts, respectively. In terms of biochemical compounds, totally 37 compounds were identified in which Ganoderic acid was observed as most abundant (15890.1 ± 232.1 μg per g dry matter) followed by Sinapic acid and Succinic acid (2011.4 ± 28.11 and 1505.33 ± 31.5 μg per g dry matter) were the predominant compounds. The results of antioxidant assays clearly revealed that, the methanolic extract proved to have higher antioxidant potential than one corresponding hot water extract for all assays. In ABTS radical scavenging activity assay, ME with the best antioxidant activity (IC50,48.46±2.42 µg/ml) had a higher activity which was significantly different (P ≤ 0.05) from HWE (163.51±4.51 µg/ml). For DPPH assay, radical scavenging capacity was dose-dependent and IC50 values of ME (111.93±1.39 µg/ml) and HWE (213.48±5.42µg/ml) was a significant difference (P ≤ 0.05). In FRAP assay, The highest level of iron-reduction was observed in the highest level of ME(IC50, 308.13±4.13 µg/ml). This extract had higher iron-chelating activity (IC50, 671.75±5.66 µg/ml) as well. These values in both assays were significantly more potent than HWE (P ≤0.01). In SO assay, ME extract (IC50, 488.8±7.38 µg/ml) and HWE (IC50, 645.92±5.48 µg/ml) showed no difference significantly. In addition, in the NO assay, both extracts released slight weak activity for neutralization of nitric oxide radicals, however, the highest activity level was related to ME (IC50, 1189.5±8.5 µg/ml) in comparison to HWE (IC50, 1343.2±10.6 µg/ml) that was significant (P ≤0.01). The results clearly indicate that different solvents used in this study significantly affected antioxidant capacities and total biochemical contents.Conclusions: G. lucidum, as a high medicinal value mushroom, proved is a very important source of nutrients and antioxidant compounds such as terpenoids, especially triterpenoids, and polysaccharides. The free radical scavenging properties, reducing power and iron-chelating inhibition of G. lucidum seemed to be correlated with phenolic compounds and triterpenoids mostly. Therefore, based on the nutritional and biochemical profile of G. lucidum, and also its antioxidant power, this mushroom possesses a high nutrient potential that reflects positively on its health benefits.
Research Article
Medicinal Plants
Behzad Kaviani; Rana Mohammadipour; Davood Hashemabadi; Mohammad Hossein Ansari; Rasoul Onsinejad; Ahmad Reza Berimavandi
Abstract
IntroductionDamask rose (Rosa damascena Mill.) is used as a multi-purpose species (edible, medicinal, aromatic and ornamental). The flower essential oil of this plant has many applications in various industries. There is a wide variety of morphological, phonological, flower shape, yield and yield of ...
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IntroductionDamask rose (Rosa damascena Mill.) is used as a multi-purpose species (edible, medicinal, aromatic and ornamental). The flower essential oil of this plant has many applications in various industries. There is a wide variety of morphological, phonological, flower shape, yield and yield of essential oils among genotypes and different populations of Damask rose in various ecological conditions in Iran. Evaluation of genetic diversity among Damask rose of Iran is important in order for breeding purposes. Some studies on phenotype, essential oil and genetic diversity were also carried out among different cultivars of rose flowers in other parts of the world. Significant diversity has been reported among the populations and genotypes of rose in different ecological conditions for many traits. Identifying superior species, cultivars and populations is important for commercial cultivation and more essential oil production. It is difficult to understand genetic diversity in roses because natural hybridization and spontaneous mutations with high abundance occurs in this plant. Morphological differences can be due to the geographical coordinates, natural hybridization and mutations. Therefore, it is important to identify the genetic diversity among the Iranian rose flowers to improve the genotypes of these flowers with appropriate garden features. The purpose of this study was to investigate and compare the genetic variety of Damask rose in Guilan, Ilam, Golestan, Tehran and Kashan in order to introduce superior genotype based on essence content and some other morphological and physiological traits.Materials and MethodsFive genotypes of Damask rose including Kashan, Ilam, Golestan, Tehran and Guilan genotypes were evaluated as plant materials. Plant materials were collected from mentioned-above regions as root-sucker and transferred to the farm of Research Institute of Forests and Rangelands of the country. The design used was a completely randomized block design, which was performed in 3 replications and was considered for each 5-suckers’ repetition (total: 75 suckers). In each replication, three specimens of each genotype were planted in pits with diameter and depth of 50-60 cm. The distance between scions per rows was 2.5 meters and row spacing from each other was 2 meters. During the experimental period, the bushes were irrigated using drip (trickle) irrigation method. The sampling was performed to measure morphological and physiological parameters after the blooms were opened in early May. Evaluated parameters were plant height, leaf length, leaf width, leaf area, petal number, stamen number, carpel number, fresh weight of petals, petal anthocyanin levels, petals essential oil levels, chlorophyll content and leaf carotenoids. Data were analyzed by ANOVA and, if significant, Tukey analysis was used. SPSS software was used for statistical analysis.Results and DiscussionThe results showed that the highest amount of essential oil (0.042 and 0.038%) was extracted from the petals of Ilam and Kashan genotypes, respectively. The highest petal weight (2.70 and 2.30 g) was related to the petals of Ilam and Kashan genotypes, respectively. The highest petal length and width were obtained in these two genotypes. The largest number of petals (71.80 per each plant) was related to Guilan samples. The highest amount of chlorophyll a was related to Ilam genotype and the highest amount of chlorophyll b, carotenoids and anthocyanin was related to Kashan genotype. Stamen number, carpel number, leaf area, leaf length and plant height were also measured. In the present study, rose flower genotypes collected from different parts of Iran showed significant diversity in relation to morphological and physiological properties, especially essence. The results of the present study showed that there was a significant correlation between the amount of essence in the petals and the weight and dimensions of the petals, so that the Ilam and Kashan genotypes with more essence in their petals had higher weight and dimensions. Similar findings related to the correlation between flower yield and its components in roses flowers were presented in some studies. The number of flowers per branch, the fresh weight of each flower and the number of flowers per branch can be a suitable choice for improving flower yield in rose flower genotypes. Some studies have shown that the weight of the flower has a very strong, positive and significant correlation with the flower yield. The findings of the present study confirm the results obtained in these studies. Despite the geographical distance between some genotypes, the high similarity coefficient between them may indicate the common origin or continuous and purposeful genotypes. On the other hand, the low similarity coefficient between genotypes proposes relatively low geographical connection and different primary origin. In the present study, there was a low correlation between the amount of essence in the petals and the weight and dimensions of the petals in the Ilam and Kashan genotypes with the Golestan and Guilan genotypes. Finding the high correlation between the more important traits is of high value in breeding programs and the selection of superior genotypes. Some reports have been presented to compare rose flower genotypes based on morphological properties. Generative traits, including flower characteristics, are more suitable for genetic and evolutionary evaluations than vegetative traits. The results of some researchers in Iran and elsewhere in the world showed that flower yield per plant is associated with some other traits, including flower number, dimensions and weight of flowers, and the number of branches in the plant. The genetic analysis of rose flower genotypes showed that some genotypes collected from different areas are genetically relevant and some are separate. This subject shows effective role of ecological conditions in changing and variability of different species and varieties. The results indicated that the difference in the amount of essential oil compounds is mostly influenced by environmental and physiological factors. Some studies have shown significant diversity in essential oil efficiency and yield in different samples of rose flowers. The morphological difference between the different genotypes of this flower indicates the presence of germplasm and the proper capacity to improve the traits and the ability to select the best genotypes using morphological markers to enhance flower yield in the country. Overall; Ilam and Kashan genotypes are suggested as superior genotypes for breeding programs.
Research Article
Pomology
Saeedeh Keivanfar; Davood Hashemabadi; Behzad Kaviani
Abstract
Introduction Olive (Olea europea L.) fruit ripening is a slow and long process and has a great impact on fruit quality, including the amount of oil. Also, interrupting the harvest and extraction of olive oil causes unfavorable conditions in this fruit. Therefore, it is important to determine the best ...
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Introduction Olive (Olea europea L.) fruit ripening is a slow and long process and has a great impact on fruit quality, including the amount of oil. Also, interrupting the harvest and extraction of olive oil causes unfavorable conditions in this fruit. Therefore, it is important to determine the best time to harvest the fruit. Olive oil has unsaturated fatty acids and has an antioxidant activity. The analysis of maturation stages is a prerequisite for fruit harvest time in each cultivar and quality of olive products. The time of harvest and maintenance after harvest is two important factors in the quality and quality of olive fruit oil. The exact determination of olive harvest time depends on the geographical area, cultivar and climate, agronomic and fruiting conditions. Study on different olive cultivars in different regions of Iran and the world revealed that fruit harvest time plays an effective role on the morphological, physiological and metabolic parameters of fruit. The approximate time of olive fruit harvest is in different geographical points, November and October. The purpose of this study was to investigate the right time of olive fruit harvesting 'Arbequina' and 'Yellow' cultivars for obtaining maximum quality of fruit and oil.Materials and Methods A factorial experiment containing two factors; cultivar in two levels ('Yellow' and 'Arbequina') and harvest time in six levels (24th and 31th October, and 7th, 14th, 21th and 28th November) based on a completely randomized block design with two factors in 12 treatments, 3 replicates and 36 experimental units was done to determine the appropriate harvest time and its effect on oil quality. Physiologic parameters; percentage of oil, amount of phenolic compounds, degree of peroxidation, amount of oleic acid, force of separation of fruit from tail and acidity were evaluated. This research was conducted at the olive research station in Rudbar city in southern Guilan province using the removed olives from the Manjil ETKA station. The 6 trees from two cultivars; 'Arbakkin' and 'Yellow' (from each 3 tree) which were similar in terms of height, age, crown diameter, mean conditions and irrigation were evaluated. Trees were planted at 6 × 8 m intervals. After selecting trees, from each tree, 2 to 3 kg of olive was randomly harvested. In fruits with tail, the force needed to separate the tail of the fruit was measured by the force assessment device. Standard method numbers 4178 and 4179 standard institutes and industrial research of Iran were used to measure acidity and olive oil peroxide, respectively. Polyphenols were measured with spectrophotometer. To determine the percentage of oil, saccule was used. For measurement of oleic acid, gas chromatography (GC) was used. Data were analyzed using SAS software and their average comparison was done by Duncan.Results and Discussion Mean comparison of the interaction effect between cultivar and harvest time showed that the highest acidity of the fruit was obtained in 'Arbequina' cultivar, respectively harvested at two times 31th and 24th October. The highest fruit peroxide value and the highest percentage of oleic acid were calculated in 'Yellow' cultivar in 24th October. The highest value of polyphenol was obtained in 'Yellow' cultivar on 7th November. The highest percentage of fruit oil was obtained in 'Arbequina' cultivar on 31th October. The lowest force to separate the tail from the fruit was applied in 'Arbequina' cultivar on 31th October. The results showed that the best time to harvest 'Yellow' cultivar is 7th November and for 'Arbequina' cultivar is 14th November. The study on several olive cultivars in China showed that the most suitable fruit harvest time was in late October until mid-November. There was an adverse significant correlation between changes in total sugar content in fruit and leaf and oil accumulation in the fruit. The quality of fruit depends mainly to the type of cultivar, genetic characteristics, maturity and environmental conditions. Study on some olive cultivars showed that the ratio between sugars is different in various stages of fruit maturity and between different cultivars of olive fruit. Some studies have shown that the most suitable time of olive fruit harvest for canned preparation is early September and for extraction of oil, late September. Fruits should be harvested when they have the highest oil accumulation. The study on 'Koroneiki' and 'Mission' cultivars in Gorgan region showed that the amount of oil in the dry matter and the percentage of free fatty acids increased with increasing degree of maturity, while peroxide value was reduced. One of the causes of peroxide value reduction during maturity is reduction in lipoxygenase enzyme activity. This enzyme increases the peroxide value by effect on linolenic acid and linoleic acid. Based on these results, the best time to harvest for the above cultivars is early in December.
Research Article
Growing vegetables
Zahra Khalili; Fatemeh Nekounam; Taher Barzegar; Zahra Ghahremani; Maliheh Farhangpour
Abstract
Introduction: Tomato (Solanum lycopersicum L.) belongs to the Solanaceae family, which is one of the most widely cultivated and economically important vegetables in the world, which is an excellent source of ascorbic acid and has high antioxidant capacity against oxidative damage caused by free radicals. ...
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Introduction: Tomato (Solanum lycopersicum L.) belongs to the Solanaceae family, which is one of the most widely cultivated and economically important vegetables in the world, which is an excellent source of ascorbic acid and has high antioxidant capacity against oxidative damage caused by free radicals. Ascorbic acid (AsA) is a water-soluble vitamin that plays a key physiological role in scavenging reactive oxygen species (ROS), and enzyme cofactor. Ascorbic acid is antioxidant and antistress agent, and also acts as a signaling molecule in some plant physiological processes and defense mechanisms. Positive roles of such antioxidants in scavenging or chelating the free radicals and activating the natural resistance against different biotic and abiotic stresses have been reported in several fruit trees. Calcium has a vital role for normal growth and development of plants due to an important role in balancing membrane structures, increasing nutrient uptakes and activates of metabolic processes. Calcium plays a vital role in maintains cell wall stability, integrity and determining the fruit quality. To our knowledge, however, little information is available regarding the interaction effect of ascorbic acid and calcium chloride on tomato. Thus, the aim of this study was to investigate the foliar application of ascorbic acid and calcium chloride on quality and antioxidant capacity of tomato fruit.Materials and Methods: To study the effect of foliar application of calcium chloride (Ca) and Ascorbic acid (AsA) on growth, yield and fruit quality of tomato, the field experiment was carried out from June to September 2021 at Research farm of faculty of Agriculture, at the University of Zanjan, Iran. Each treatment was carried out with three replicates. Different concentrations of Ca (0, 0.3, 0.6 and 0.9 %) and AsA (0, 100, 200 and 300 mg L-1) were sprayed three times (0, 15, 30 days after full bloom). Fruits were harvested at two harvests stage (orange and red color) and transferred to the laboratory on the same day. Flesh firmness was determined with penetrometer (model Mc Cormic FT 32), using an 8 mm penetrating tip. Results were expressed in kg cm-2. The pH values of solutions were monitored with pH meter. TSS was measured in the extract obtained from three fruit of each replicate with a digital refractometer Atago PR-101 (Atago Co., Ltd., Tokyo, Japan) at 20◦C. Total ascorbic acid content was expressed as mg per 100 g of juice. Antioxidant activity was measured using the free radical scavenging activity (DPPH) and calculated according to the following formula: RSA%= 100(Ac-As)/Ac. Statistical analyses were performed with SPSS software package v. 20.0 for Windows, and means comparison were separated by Duncan’s multiple range tests at p <0.05.Results and Discussion: The results showed that fruit harvested at red color stage had the higher vitamin C, total soluble solid (TSS), total phenol, flavonoids contents and antioxidant capacity compared to fruit harvested at orange color stage. Foliar application of AsA and Ca had significantly improved tomat fruit quality. The highest value of TSS (4.9 °B), vitamin C (46.1 mg 100ml-1), total phenol and flavonoids contents and antioxidant capacity (36%) was achieved with application of 300 mg L-1 AsA and 0.9% Ca in fruit harvested at red color harvest time. The lowest value of pH and highest TA was observed in red color fruit treated with 300 mg L-1 AsA and all Ca levels. Ca had significant effect on fruit firmness, which the highest fruit firmness was obtained from 0.9% Ca. The fresh tomato is an important source of ascorbic acid for human consumption. AsA significantly increased the amount of vitamin C in the plum and sweet pepper fruits. Increasing vitamin C content in fruits after treatment with Ca could be related to inhibiting action of calcium on the activities of ascorbic acid oxidase that use ascorbate as a substrate. The results indicated that treatment of Ca produced fruits with higher firmness compared to control and other treatments. Firmness and resistance to softening can be increased by the addition of Ca, due to interaction of calcium with pectate acid in the cell wall to form calcium pectate and retarding polygalacturonase activity. Differences in the percentage of TSS content at the time of harvest indicated the AsA and Ca effects on carbohydrate accumulation in fruits, which had different potential on respiration rates and consequently storability of plants. The antioxidant activity has positive correlation with total phenolic content, flavonoids and content of ascorbic acid. Conclusion: The results of our research indicated that per-harvest foliar application of AsA and Ca improved fruit quality attributes including vitamin C, fruit firmness, TSS and antioxidant activity. These results suggest that AsA and Ca treatments, especially AsA 300 mg L-1 and Ca 0.9%, may be proposed to improve fruit quality.
Research Article
Postharvest physiology
Davood Hashemabadi; Amirhossein Soleimani; Behzad Kaviani; Nayyereh Naziri Moghaddam
Abstract
IntroductionCut carnation flowers have high economic importance in the floriculture industry. The postharvest life of cut ornamentals, including carnation, is important in determining their quality and consumer preference. High production of ethylene is one of the most important factors that reduce the ...
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IntroductionCut carnation flowers have high economic importance in the floriculture industry. The postharvest life of cut ornamentals, including carnation, is important in determining their quality and consumer preference. High production of ethylene is one of the most important factors that reduce the postharvest life of this flower. High ethylene production causes the aging of some cut flowers. In order to increase the vase life of cut flowers, many researches have been done and various preservative solutions have been introduced, and in some of these solutions, disinfectants have also been used. One of the effective compounds in stopping or reducing ethylene production are alcohols. Ethanol stops ethylene synthesis in cut carnation. Cut carnation is climacteric flower and sensitive to high levels of ethylene. Therefore, the aim of this research was to investigate and compare the effect of different amounts of propanol, butanol and pentanol on the vase life and quality improvement of cut carnation flowers cultivar 'Nelson'.Materials and MethodsIn the present study, propanol, butanol and pentanol were used at the concentrations of 2, 4 and 6% for 24-hour pulse, in order to reduce ethylene production and increase the vase life of cut carnation cultivar 'Nelson' flowers. In February 2020, cut carnation flowers that were harvested at the commercial stage were prepared from a greenhouse in Mahalat city and were immediately transferred to the Postharvest Laboratory, Faculty of Agriculture, Islamic Azad University, Rasht Branch for treatment and evaluation of traits. This experiment was conducted as a completely randomized design in 30 plots. For each treatment, 3 replications and 5 samples for each replication and a total of 150 flower branches were considered. Some traits such as vase life, water absorption, dry matter, petal protein, leaf chlorophyll, ethylene production, fresh weight loss, flower opening index, Brix degree, lipid peroxidation and activity of antioxidant enzymes, peroxidase and superoxide dismutase were measured. To evaluate the vase life of cut flowers, the main criterion is the turning of the petals inward and the apparent wilting of the flowers. Data analysis was done using SAS statistical software and mean comparison based on Duncan's multiple range test.Results and DiscussionThe results of variance analysis of the data showed that the effect of different amounts of propanol, butanol and pentanol application was significant at 5% and 1% probability level on vase life and most of the traits measured in cut carnation flowers. This research revealed that propanol had a more effective role in reducing ethylene production and increasing the vase life than butanol and pentanol. The results showed that the longest vase life (14.11 days) was observed in cut flowers treated with 2% propanol. This treatment also caused the highest amount of water absorption, percentage of dry matter, petal protein and leaf chlorophyll, as well as the lowest amount of ethylene production. The lowest vase life (8.91 days) was observed in control cut flowers. The mean comparison of the different alcohol treatments showed that all treatments of propanol and butanol alcohols in different concentrations increased the absorption of solution by cut flowers compared to the control. Maintaining the water balance in cut flowers is one of the important factors in the vase life of cut flowers. The content of water uptake by cut flowers depends on the hydraulic conductivity of the water channel in the stem and the water potential difference between cut flower tissues and the preservative solution. The least plasma membrane damage was caused in cut flowers treated with 2% propanol. This treatment also caused the highest SOD activity. The short vase life of cut flowers is a global challenge, and efforts are being made to increase their vase life by using appropriate compounds in the vase solution. One of these compounds are alcohols, which have been used by some researchers in order to increase the vase life of various cut flowers. Alcohols are usually toxic for cells in high concentrations. Propanol and butanol in low concentrations inhibited the synthesis of ethylene and therefore increased the vase life of cut carnation flowers. These anti-ethylene compounds prevent the senescence of the petals, which is usually associated with the browning of the petals. Alcohols in optimal concentrations act as a signal and increase the vase life of cut flowers and improve their postharvest quality by reducing lipid peroxidation and inducing the activity of antioxidant enzymes. Similar findings were reported in cut alstroemeria, some carnation cultivars and chrysanthemum flowers.ConclusionThe present study showed the positive effect of disinfectant and antimicrobial compounds on increasing the vase life, some physiologic characters and antioxidants activity. This research revealed that propanol had more effective role in reducing ethylene production and increasing the vase life than butanol and pentanol. The results showed that the longest vase life was observed in cut carnation cv. Nelson flowers treated with 2% propanol.
Research Article
Medicinal Plants
Hoda Sajedimehr; Mahya Sajedimehr; Majid Azizi
Abstract
IntroductionMedicinal plants with a high level of antioxidant activity are of great importance due to their effect on a wide range of diseases such as diabetes, inflammatory diseases, Parkinson's, Alzheimer's, cardiovascular diseases, blood pressure and other diseases caused by oxidative stress. Therefore, ...
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IntroductionMedicinal plants with a high level of antioxidant activity are of great importance due to their effect on a wide range of diseases such as diabetes, inflammatory diseases, Parkinson's, Alzheimer's, cardiovascular diseases, blood pressure and other diseases caused by oxidative stress. Therefore, studying medicinal plants and extracting and identifying chemical properties and effective compounds in medicinal plants emphasizes the necessity of learning and using medicinal plants. Iranian shallot plant with the scientific name Allium hirtifolium is a species of the onion family (Alliaceae) and is one of the largest genera of the monocot group. Shallot plant is an important horticultural product that has been known and used as a vegetable, spice and medicinal plant since ancient times due to its useful properties. In this genus, there are economically important species such as: onions, garlic, shallots, chives and leeks. Germination and sprouting are one of the most important phenological stages (life cycle) of the plant. Having enough information about the phenological cycle of the plant leads to better management of the different stages of this cycle, which determines the degree of success of agricultural systems. Seedling germination and growth in the early stages are strongly influenced and controlled by environmental factors, especially temperature, soil moisture and seed quality (germination and seedling strength) and internal factors (growth regulators) such as Gibberellin and Abscisic acid hormones.In order to speed up the germination of seeds and eliminate seed dormancy so that germination can happen faster, there are different methods. One of these methods is mechanical or chemical scratching. The aim of this research is to present a precise and scientific description for the failure of Iranian shallot medicinal plant seed dormancy (Allium hirtifolium) and improvement in the acceleration of germination, considering the different characteristics in three populations of Iranian shallot seeds. Also, to increase the quality and accuracy in this research, a scanning electron microscope was also used to accurately determine the surface changes of the seeds before the acid-washing treatment with sulfuric acid and after the acid-washing treatment with sulfuric acid. In the previous research that has been carried out on Iranian shallot seeds, the simultaneous examination of different temperatures and different times of acid washing by sulfuric acid in a precise manner and with several populations that have different genotypes, as well as the use of Scanning Electron Microscopy (SEM) has not been done.Materials and methodsThe study was done to investigate the effect of different degrees of temperature, and different times of sulfuric acid for acid washing on the improvement of germination of three populations of Iranian shallot seeds (Allium hirtifolium). The effect of four different temperatures (5, 10, 15 and 20 degrees Celsius) inside the Germinator device and four different times of acid washing with sulfuric acid (0, 5, 10, 15 minutes) on germination and micromorphological characteristics of three populations of shallot plant (Feridon Shahr, Tiran and Khansar) was investigated. On the other hand, a scanning electron microscope (SEM) with different degrees of magnification was used for the effect of sulfuric acid treatment on the surface of the seed coat. The experiment was conducted as a factorial, in the form of a completely randomized design with four replications. At the end of the experiment, traits such as germination percentage, average germination time, germination speed, root length and shoot length were evaluated. Also, traits such as diameter, length, volume and weight of seeds in three populations of shallot were also analyzed statistically.Conclusion and discussionThe results of electron microscopy showed that in all three populations of Iranian shallot seeds, the seed coat, after using the acid washing treatment with sulfuric acid for 15 minutes, underwent noticeable and significant changes compared to the control seeds. The surface of the seed is destroyed by sulfuric acid and the surface of the seed does not have its natural ornamentation and protrusions compared to the control. Also, the effect of temperature, duration of acid washing and seed population on germination percentage, germination speed, average germination duration, seedling root length and seedling length was significant at the 1% probability level (p≤0.01). The population of Fereydoun Shahr has the highest germination percentage (69.16 percent), germination speed 3.7 (number of seeds per day) and average duration of germination 9.64 (number of seeds per day) at a temperature of 5 degrees Celsius and acid washing time of 15 minutes. The maximum shoot length and root length were 19.7 and 8.81 (mm) in the Tiran population with a temperature of 20 degrees Celsius and a 15-minute acid washing time, and in the Khansar population with a temperature of 20 degrees Celsius and time of 5 minutes acid washing respectively. The results show that, according to the dormancy of the seeds and their need for cold, the best temperature for shallot seed germination is 5 degrees Celsius with 15 minutes of acid washing by sulfuric acid. Diversity in shallot plant populations has a significant effect on the performance of this plant in improving seed germination.AcknowledgeWe thank Ferdowsi University of Mashhad and the Isfahan University of Technology.Keywords: shallot diversity (seed morphology), stratification, seed scarification, germination, scanning electron microscope (SEM)
Research Article
Pomology
Fatemeh Nekounam; A. Salehi
Abstract
Introduction Apple (Malus damestica L.) is one of the most popular temperate fruits in the world as well as Iran. One of the challenges in managing apple orchards is excessive fruit formation or excessive fruit drop especially in the pre-harvest stage, which affects the performance and marketable product. ...
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Introduction Apple (Malus damestica L.) is one of the most popular temperate fruits in the world as well as Iran. One of the challenges in managing apple orchards is excessive fruit formation or excessive fruit drop especially in the pre-harvest stage, which affects the performance and marketable product. Therefore, the control fruit set is required to help regular fruit production. Currently, the application of synthetic auxins as plant growth regulators are successfully practiced and mainly used in various countries, including all aspects of modern apple production to control and manipulate vegetative growth and regulation of flowering, reduce immature fruit drop, fruit maturity, firmness, and manage apple harvest. Among the auxin-type growth regulators, NAA is a synthetic auxin analogue that may down-regulate abscission-related genes and reduce the sensitivity of the abscission zone to ethylene. It has long been used to reduce or totally prevent pre-harvest fruit drops, to preserve fruit flesh firmness, and to prevent starch degradation in apples. Therefore, this experiment was conducted to evaluate the effects of NAA foliar application at different days after full bloom on controlling fruit drop and improving physical and biochemical attributes of ‘Red Delicious’ apple fruits. Findings described here may help to understand the effects this PGR on apple quality and also help on the development of strategies to reduce post-harvest losses of apple fruits.
Materials and Methods In order to study the effect of NAA on controlling fruit drop, fruit yield and quality, a factorial experiment based on randomized complete blocks design with three replications was conducted under Zanjan climatic conditions during 2023. Ten year-old ‘Red Delicious’ Standard apple trees grafted on M.9 rootstock were used as plant material. Treatments consisted of different concentration of NAA (15, 30, 50 and 75 mg L-1) sprayed at different days after full bloom (10, 25 and 40 DAFB). The date of full bloom was 04/22/2023. The fruit growth pattern was based on fruit weight and diameter during DAFB. According to fruit growth pattern, fruit were harvested at physiological maturity stage. Fruit number per replicate, fruit weight, diameter and shape index, fruit firmness, total soluble solids content and taste index were measured. Also, fruit drop percentage, fruit yield as well as fruit efficiency was estimated.
Results and discussion Advanced knowledge of apple fruit development from fruitlet to maturity is crucial for optimal prediction of year-to-year yields and fruit quality. Apple fruit growth has been defined as sigmoidal increase in fruit diameter or fresh weight. Studying two fruit growth patterns based on fruit weight and diameter data showed that fruit weight is a more appropriate indicator for drawing fruit growth patterns, and according to that, the fruits were harvested at the physiological maturity stage. Foliar spray of NAA during different DAFB showed different significant effects on fruit drop and yield efficiency. The highest rate of fruit drop (95.7 % and 85.9 %) was observed with foliar application of NAA at 10 and 25 DAFB, respectively, but its application at 40 DAFB reduced fruit drop by 46.9% compared to the control, and correspondingly the fruit yield increased by 22% compared to the control treatment. The lowest number and weight of fruits per unit shoot cross sectional area was observed in trees treated with different concentrations of NAA at 10 DAFB. The maximum fruit length (72.3 mm), diameter (75.8 mm), weight (180 g) and fruit volume (240 cm3) were obtained with application of 75 mg L-1 NAA at 10 DAFB. The foliar spray of NAA at 10 DAFB significantly increased the fruit density, flash firmness and TSS. But their use in 25 and 40 DAFB had no significant effect on fruit density and flash firmness, and significantly decreased fruit TSS by 23.9% compared to the control trees. Unlike the TSS, the value of fruit titratable acidity showed a significant increase in all three times of naphthalene acetic acid treatment compared to the control, and as a result, the fruit taste index decreased.
Conclusions The present study concluded that the positive effects of NAA in controlling fruit drop and improving the yield and fruit quality will be different depending on the time of application and the weather conditions of the region under study. According to the results, the application of NAA at 40 DAFB reduced fruit drop and increased fruit yield efficiency.
Research Article
Pomology
Mansour Jalali; Norollah Moallemi; Esmaeil Khaleghi; Shoreh Zivdar; Mostafa Rahmati-Joneidabad
Abstract
IntroductionThe date palm tree tolerate high temperatures, drought and salinity more than other fruit crop, that is why date palm tree is named the tree of life in the desert. Date palm (Phoenix dactiylifera) is one of the fruit tree crops that is cultivated in arid region of Middle East and North Africa ...
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IntroductionThe date palm tree tolerate high temperatures, drought and salinity more than other fruit crop, that is why date palm tree is named the tree of life in the desert. Date palm (Phoenix dactiylifera) is one of the fruit tree crops that is cultivated in arid region of Middle East and North Africa having a significant role on the economy of many countries in these regions. Fruit thinning is one of the major operation for improving the quality of dates as also for other fruit trees. Quality of dates is improved by increasing fruit weight and size and regulating date palm production by reducing the magnitude of year to year production affecting the date palm. Date palm orchard operations include all operations that are repeated annually to produce date fruit. Alternate bearing is common phenomenon in date palms. The thinning is one of the main methods that often helps to solve this problem. The main purpose of thinning is to create a suitable balance and often to increase the leaf to cluster ratio. It is creating a balance between vegetative growth and flowering and reducing the possibility of aging in the Alternate bearing. Fruit thinning is one of the necessary operations that affects the growth, quality, fruit yield and regulation of the Alternate bearing of the tree. The thinning process is performed manually, mechanically and chemically. Therefore, choosing a thinning method that saves time and money is necessary for date palms, especially in critical situations. The purpose of this experiment was conducted to investigate the effect of manual and chemical thinning on qualitative characteristics in different stages of date fruit development of the Khadrawi cultivarMaterials and MethodsAn experiment was carried out as factorial experiment based on randomized complete block design with 3 replications at Shahid Chamran University of Ahvaz in 2022 and 2023. The Experimental factors included: hand thinning (No hand thinning, removal of 33%, removal of 25% of the total number of strands) and chemical thinning (zero, 50 and 100 mg per liter of naphthalene acetic acid). Chemical and strand thinning were done in the Hubabok stage (about 4 weeks after pollination) and the Kimri stage (12 to 13 weeks after pollination), respectively. In two consecutive years, the fruits were transferred to the plant physiology laboratory of the Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz. The qualitative traits such as total soluble solids (%), pH, total acidity (%), taste index, antioxidant activity (%), total phenol (mg/gr FW), total soluble tannin (mg/gr FW), ascorbic acid (mg/gr FW), poly-galacturonase enzymes (U/gr FW), methyl pectin esterase (U/gr FW), cellulase (U/gr FW) and invertase (U/gr FW) and the percentage of reducing (%), non-reducing (%) and total sugars (%) were measured. Results and DiscussionThe results of this experiment showed that in the Tamar Stage, the interaction of hand and chemical thinning improved the qualitative characteristics of the fruit and on the characteristics of soluble total tannin, polygalactronase activity, pectin-methylesterase and reducing and total sugars, and the effect of hand thinning on total and percentage phenol and Non-reducing sugar and chemical thinning had a significant effect on ascorbic acid and cellulase enzyme. The results showed that there was upward trend in amount of total soluble solids, pH, total acidity, taste index, poly-galactronase, pectin-methyl-esterase, cellulase and invertase enzymes and the reducing and total sugars from the Kimri to Tamar stage. Also, there was downward trend in amount of total phenol, total soluble tannin content, ascorbic acid, and non-reducing sugar percentage from the Kimri stage to the Tamar.ConclusionsThe Khadrawi date variety has short strands, which results in a significant density of fruits on the strands of a cluster, which leads to large changes between fruits in most of the biochemical properties and has a negative effect on marketability. Fruit thinning is one of the most important agronomic works in the groves, which improves the quality characteristics of the fruit. In general, the although, hand thinning is more expensive, time-consuming and relatively more difficult than chemical thinning, but our finding showed that the quality of date fruit of Khazrawi cultivar improved by removing 25% of the total number of strands and chemical thinning with 100 mg/liter naphthalene acetic acid and these treatments is recommended for palm growers.
Research Article
Growing vegetables
Keyvan Poorhossein; Bahram Abedy; Mahmood Shoor
Abstract
Introduction Urban agriculture is one of the basic characteristics of urban schematization, which helps to increase the quality of urban life via reducing the climate pollution, preserving and increasing biodiversity, as well as re-utilizing urban waste. Therefore, in recent years, urban agriculture ...
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Introduction Urban agriculture is one of the basic characteristics of urban schematization, which helps to increase the quality of urban life via reducing the climate pollution, preserving and increasing biodiversity, as well as re-utilizing urban waste. Therefore, in recent years, urban agriculture has gained significant importance due to its economic, social and cultural benefits. However, studies have indicated that urban soils can contain high concentrations of certain infrequent elements such as lead and cadmium. These heavy metals can be distinguished from other pollutants because they cannot be bio-degraded; but they accumulate in living organisms and subsequently cause diseases and various disorders even in relatively lower concentrations. Plants, like other organisms, have defense mechanisms that are activated in stressful conditions. Thus, under stress, enzymatic antioxidant systems and non-enzymatic metabolites enhance, due to the production of reactive oxygen species. Peppermint plant (Mentha piperita L) has an essential oil with medicinal properties. High levels of heavy metals cause chlorosis and necrosis, increase the accumulation of reactive oxygen species (ROS) and oxidative stress in mint plants. The present study was conducted on a regional scale in order to investigate heavy metal pollution and determine their impacts on some physiological and biochemical traits as well as the concentration of Cd and Pb in peppermint plants grown in urban green spaces.Material and methodsThis study was conducted to assess the amount of heavy metal absorption and its effect on some biochemical properties of peppermint plant (Mentha piperita L.), in Mashhad city in 2021.The experiment was carried out as a factorial in the form of randomized complete block design in three replications. The first factor (location) were Faz4 Park (with high degree of contamination) and Nasim Park (with low degree of contamination). The second factor was the times of harvest (June15, July15 and August 15). At the time of every harvest fully developed leaves were collected to evaluate physiological and biochemical traits.Results and discussionThe results of analysis of variance indicated that the effect of location was significant on all traits except for the yield of essential oil. Also, the effect of harvest time was significant on all traits except for peroxidase activity and the yield of essential oil. However, the interaction of location and harvest was significant only on phenol, flavonoid, proline, cadmium and lead concentration. The results indicated that the ascorbate peroxidase, catalase and peroxidase activities were higher in Faz 4 Park. Moreover, the highest activities of ascorbate peroxidase, catalase and peroxidase were recorded in Faz 4 + first harvest. Heavy metals cause the production of reactive free radicals and also increase the activity of antioxidant enzymes. However, the chlorophyll a, b, carotenoid and total chlorophyll contents were higher in Nasim. Thus, the highest contents of Chla, Chlb, Chltotal and carotenoid were observed in Nasim + first harvest. The higher amount of chlorophyll and carotenoids in the first harvest is due to the optimal growth conditions such as day length and sunlight and ambient temperature. In addition total phenol, flavonoid, proline, Cd and Pb elements indicated a reducing trend in Faz 4 compared to Nasim Park in different harvest times, but the amount of these traits were higher in the first harvest than in the subsequent harvests. Increased amount of total phenol in the first harvest can be related to the high air temperature at the first harvest which caused stressful conditions in this stage. Proline production also improves under heavy metal stress in order to protect the plant against toxicity. Nevertheless, the percentage of essential oil showed an increasing trend via enhancing the absorption of Pb and Cd in Faz 4 compared to Nasim Park. The higher percentage of essential oil in Faz 4 can be due to the lower growth of leaves due to the presence of more heavy elements in this area. In general, despite the fact that only the concentration of Pb was higher than the world standard level in both parks, the contamination with Cd and Pb (especially Pb) was more in Faz 4 than in Nasim Park, which was a factor in diminishing the growth traits of peppermint plants. In a case of harvest times, the first harvest recorded better growth characteristics and higher absorption of heavy metals due to the higher strength of the plant, while in the third harvest, due to spending more energy for regrowth, it had lower growth characteristics and weaker absorption of Pb and Cd metals. It was cadmium.
Research Article
Medicinal Plants
Behrooz Rahimkhani; Mahboobeh Naseri; Ahmad Ahmadian; Masoud Alipanah
Abstract
Introduction:From the past, medicinal plants have been used as one of the most important resources for medicinal purposes. Even now, the use of medicinal plants is expanding in many developed. Salinity stress is one of the most important influencing factors in reducing plant growth. Salinity stress limits ...
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Introduction:From the past, medicinal plants have been used as one of the most important resources for medicinal purposes. Even now, the use of medicinal plants is expanding in many developed. Salinity stress is one of the most important influencing factors in reducing plant growth. Salinity stress limits plant growth by reducing metabolic and physiological activities in the plant. One of the consequences of salinity stress in the plant is the production of abscisic acid in the plant. One of the methods that have been tested in recent years to reduce the effects of salinity stress in plants is the use of seaweed and its extract. According to the studies conducted in some plants, seaweed extract can cause the growth and expansion of the roots and help to increase the absorption of water and minerals through the roots. Also, based on the research conducted on some plants, the use of seaweed increases the amount of chlorophyll in the plant and accelerates the time of flowering and fruit formation in the plant. Echium amoenum is a perennial plant belonging to the family Borage is a valuable plant in terms of its medicinal properties is considered. in general environmental factors have a significant effect on flower production in these plants. Therefore, for the successful cultivation of medicinal plants, including in general environmental factors have a significant effect on flower production in these plants. Therefore, for the successful cultivation of medicinal plants, including Echium amoenum, providing optimal environmental conditions is a priority, providing optimal environmental conditions is a priority.Ascophyllum nodosum seaweed extract contains significant amounts of high-use mineral elements such as nitrogen, potassium, calcium, magnesium, and low-use mineral elements such as iron, copper, and manganese. Therefore, according to the current results, in this study, the effect of foliar spraying of algae extract was investigated. The morphological characteristics of Echium amoenum seedling under salt stress were investigatedMaterials and MethodsIn order to investigate the effects of foliar spraying of seaweed extract on borage flower seedlings under salinity stress conditions, a factorial experiment was conducted with two factors of seaweed and salinity stress with sodium chloride salt, in the form of a completely randomized design in the greenhouse. The seeds were purchased from Pakan Seed Company of Isfahan and soaked in normal water for 24 hours, and then they were transferred into small pots containing three parts of peat moss and one part of perlite. One week after transferring the seedlings to the main pots, foliar spraying with seaweed extract was done. Foliar-spraying was repeated once every two weeks and in total the seedlings were sprayed three times with seaweed extract. In this experiment, a concentration of 1500 ppm of seaweed extract and three levels of salinity (EC=1.6, 4, 8) were used.The seaweed extract used in this experiment belonged to Akadin Company. The type of seaweed from which the extract was prepared was Ascophyllum nodosum and it is a type of brown algae. One week after the first foliar application of seaweed extracts, the application of salinity stress began. In order to prevent shock in plants, salinity treatment was done gradually and in three stages. In order to prevent salt accumulation, washing with ordinary water was done once every two weeks. Results and DiscussionThe results showed that the use of seaweed extract can significantly protect plant growth under salinity stress. Seaweed extract increased the amount of proline and potassium in the leaves of the plant and thereby reduced the harmful effects of salinity stress on the borage plant. In addition, foliar spraying of borage plant with the use of seaweed extract increased the amount of chlorophyll in the plant, and in this way, by increasing the amount of photosynthesis in the plant; it helped the plant to grow better under salt stress conditions. The results of this research showed that the use of seaweed extract helps the plant to maintain its conditions against salt stress by increasing the amount of proline and absorbing potassium in the tissue. In addition, foliar spraying with seaweed extract preserves the structure of chlorophyll in the plant under salinity stress, and in this way, by increasing the photosynthetic efficiency, it helps the plant grow better under salt stress. According to the obtained results, it can be concluded that the use of seaweed can reduce the negative effects of salinity stress in the seedlings of Iranian borage. In addition, due to its low price and availability, it can be used as a suitable bio-fertilizer to protect plant growth under salinity stress conditions.
Research Article
Pomology
afsaneh Salehi; Fatemeh Nekounam; Farhang Razavi
Abstract
Introduction
Apple (Malus domestica) belongs to the Rosacea family and is one of the most important fruit trees in temperate regions. Apple fruit is a rich source of vitamins, sugars, organic acids, minerals, fibers, and bioactive compounds and is widely cultivated due to its pleasant taste, aroma, and ...
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Introduction
Apple (Malus domestica) belongs to the Rosacea family and is one of the most important fruit trees in temperate regions. Apple fruit is a rich source of vitamins, sugars, organic acids, minerals, fibers, and bioactive compounds and is widely cultivated due to its pleasant taste, aroma, and texture. Due to the rising need for food and fiber per unit of land area, chemical fertilizers are becoming increasingly popular to increase yields from small plots of land. Chemical fertilizers pose major health risks and harm the environment when they are used in excess. Because of this, research in this area is heavily focused on finding and evaluating the efficiency of new products. One such approach is using biostimulants that can enhance the effectiveness of conventional mineral fertilizers. Plant biostimulants contain some nutrients (marine plant extracts, humic acids, amino acids and other natural products such as saponins and compost teas) that stimulate plant growth, even when administered in small amounts. Foliar application of seaweed extracts at 0.2% recorded maximum no. of fruits/tree, fruit weight, and yield/tree in valencia orange.
Materials and methods
In order to investigate the effect of biofertilizers on growth, yield and leaf nutrient contents of apple under climatic conditions of Zanjan, the experiment was carried out in a completely randomized block design with three replicates in 2023. Different concentration of seaweed (Alg; 0.075 and 0.15%), amino acid (GF Amino; 0.1 and 0.2%), humic acid (HA; 0.3%), commercial fertilizer Homarang, (Homa fert; 0.5%), combined chemical fertilizer (nitrogen, zinc and boron (Combinate fert; 1% urea, 0.3% zinc chelate and 0.1% boric acid)) and distilled water as a control were sprayed on the trees at 40 days after full bloom stage until runoff using a mechanical mist sprayer, and repeated three times with an 30 days interval until the physiological ripening of fruits. The experiment was carried out on 10-year-old Red Delicious apple trees grafted on M9 rootstocks. Shoot length, chlorophyll index, fruit drop percentage, yield efficiency, leaf area, leaf dry matter, leaf macro and micro nutrient were measured. The analysis of variance (ANOVA) and least significant difference test (P≤ 0.05) used to compare means within each sampling date. The Statistical analysis and standard error calculation were carried out using SAS software (V. 9.3).
Results and Discussion
The results showed that the foliar application of bio and chemical fertilizers significantly increased growth and fruit yield. So that, the highest increase in shoot length (39%) and leaf area (74.30%) compared to the control was obtained with application of seaweed 0.075%. Also, seaweed 0.15%, amino acid 0.2%, humic acid and amino acid 0.1% caused a significant increase in leaf area (42.02, 35.57, 22.27 and 16.20%, respectively) compared to the control. Foliar spray of seaweed, amino acid and chemical fertilizer increased chlorophyll index. The highest increase in chlorophyll index (50%) compared to the control was obtained with application of combined chemical fertilizer at 130 days after full bloom. These results are in agreement with the outcomes of other experiments conducted with seaweed on apple and on other crops such as grapevine. Therefore, this represents a further evidence of a possible role of seaweed extracts in the reduction of chlorophyll degradation and in delaying leaf senescence. The highest amount of nitrogen (1.66%), potassium (1.03%) zinc (150 mg g-1DW) and boron (82.5 mg g-1DW) and the lowest amount of phosphorus (0.44%) were obtained with application of combined chemical fertilizer. The highest value of iron was observed in leaf of trees treated with seaweed 0.15%, humic acid and commercial biofertilizer. Based on the results, it was observed that the amino acid, seaweed and combined chemical fertilizer have the greatest effect in reducing fruit drop (36.97, 33.37, 29.07%, respectively) compared to control) and increasing yield efficiency (respectively 2.75, 2.73 and 2.8 compared to control with 0.22 fruits No. cm-2 SCSA). These results partially are in agreement with another research performed on apple, where the use of a similar seaweed extract (Ascophyllum nodosum) was found able to induce a higher final yield. The hormonal components found in the extracts, particularly cytokinins, are assumed to be responsible for the increased yield in plants treated with seaweed. Previous studies mentioned that the application of biological fertilizers alone or in combination with the mineral fertilizers had positive influences on the leaf plate area, mean fruit weight and fruit chemical composition.
Conclusions
According to the results of this research, the use of biofertilizers, especially seaweed (0.15%) and amino acid (0.1%), are suitable and nature-friendly substitutes for chemical fertilizers and can play a significant role in increasing growth indices and yield of apples.
Research Article
Pomology
Seyed Asghar Mousavi; Akram Vatankhah; Ali Imani
Abstract
IntroductionAlmond (Prunus dulcis L.) is one of the valuable nut trees that is cultivated in many temperate regions and Mediterranean climatic conditions for domestic consumption and export. Almond belongs to the genus Prunus, from the Rosaceae family. Identifying and introducing genotypes and cultivars ...
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IntroductionAlmond (Prunus dulcis L.) is one of the valuable nut trees that is cultivated in many temperate regions and Mediterranean climatic conditions for domestic consumption and export. Almond belongs to the genus Prunus, from the Rosaceae family. Identifying and introducing genotypes and cultivars of late bloom is one of the most important goals of almond breeding programs. The correct choice of almond rootstock causes better management of the garden, compatibility with all types of soil and resistance to nematodes. Peach × almond hybrid has been the most widely used rootstock in both dry and irrigated conditions in the past years. Creating an orchard by selecting grafted genotypes on suitable rootstock for sustainable cultivation of almonds is particularly important. Cultivation of superior genotypes grafted on fruit trees has an effect on pomological characteristics, yield and quality of nuts. The requirement for the introduction and production of superior cultivars is an accurate selection between cultivars, which is possible through the identification of cultivars and their diversity. The purpose of this research is to investigate and evaluate the most important vegetative, phenological, quantitative and qualitative characteristics of nuts and kernels in 36 promising cultivars and genotypes grafted on GN15 rootstock, in order to introduce superior cultivars.Materials and Methods:In this research, 36 promising almond cultivars and genotypes on GN15 rootstock were investigated in garden conditions in terms of various vegetative traits, nut and kernel characteristics in order to obtain suitable commercial cultivars. This research was conducted at the Badam research station in Saman region affiliated to the Center for Research and Education of Agriculture and Natural Resources of Chaharmahal and Bakhtiari province as a randomized complete block design with three replications. Vegetative traits of tree height, canopy length, canopy width, and branch length were measured by meter in the garden, and rootstock diameter, scion diameter, and branch diameter were measured in the garden with calipers. In order to measure the nut and kernels, 100 fruits were harvested from each of the studied cultivars and genotypes at the time of fruit ripening, and their green shell was separated and dried. Measurement of traits such as length, width, diameter of nut and kernel was done by digital caliper and weight of nut and kernel was measured by digital scale with accuracy of 0.01. Coding of some traits was done based on almond descriptor (Gülcan, 1985) with some changes. The data obtained from the experiment were analyzed using SAS software (version 3.1.9). To compare the means, Duncan's multiple range test was used at the 5% probability level.Results and DiscussionAccording to the results of analysis of variance (ANOVA), there was a statistically significant difference at the level of 1% between the attributes of tree height, canopy width, rootstock and scion diameter, branch length and diameter, and the ratio of tree height to canopy length. (P<0.01). The results of variance analysis show that there is a significant difference between the investigated nut and kernel traits in promising cultivars and genotypes grafted on GN (Table 5). These differences show the diversity in the investigated traits and it is possible to choose cultivars for different values of the same trait. Based on the average comparison results of the vegetative traits, the highest height in genotypes GA4, GA3, GA35, The highest canopy width was observed in genotypes GA5, GA17, GA3, and GA20, GA15, GA5, the highest diameter of rootstock and scion, and the highest length and diameter of one-year branches were observed in genotype GA18. The results of the comparison of the average nut and kernel characteristics show that there is a significant difference in the cultivars and genotypes investigated in this research. The results of the comparison of the average nut and kernel characteristics show that there is a significant difference in the cultivars and genotypes investigated in this research. Based on the obtained results, cultivars and genotypes of GA5, GA24, GA12, GA9 and GA1 showed relative superiority in terms of nut and kernel traits. The results of this research showed that the GA35 genotype grafted on the GN15 rootstock had the highest length, width and diameter of the nut, and the highest weight of nut and kernel. The kernel color light, the without shrinking the kernel and the highest percentage of kernel and the highest ratio of kernel weight to nut weight.ConclusionThe results of this research showed that the examination of vegetative traits, nuts and kernels in the studied cultivars and genotypes could show the diversity between cultivars and genotypes. The results showed that the investigated cultivars and genotypes have significant differences in terms of all nut and kernel traits, which indicates the existence of diversity between the investigated cultivars and genotypes. This indicates that these cultivars and genotypes can be considered as a source of good germplasm for breeding programs. Cultivars and genotypes with higher percentage of kernels had thinner shell, more patterns on the skin, and light to medium kernel color. Based on the obtained results, cultivars and genotypes of GA5, GA24, GA12, GA9 and GA1 showed relative superiority in terms of nut and kernel traits. The results of this research showed that the GA35 genotype grafted on the GN15 rootstock had the highest length, width and diameter of the nut, and the highest weight of nut and kernel. The kernel color light, the without shrinking the kernel and the highest percentage of kernel and the highest ratio of kernel weight to nut weight.
Research Article
Growing vegetables
Farhad Shakarami; sadegh Mousavi-Frad; Abdolhossein Rezaei Nejad; Farhad Beiranvand
Abstract
IntroductionSalinity in water and soil stands as a crucial environmental factor that significantly hampers global agricultural production. Over recent decades, the escalating demand for irrigation in arid and semi-arid regions has intensified this issue, making it a major agricultural challenge. Salinity ...
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IntroductionSalinity in water and soil stands as a crucial environmental factor that significantly hampers global agricultural production. Over recent decades, the escalating demand for irrigation in arid and semi-arid regions has intensified this issue, making it a major agricultural challenge. Salinity stress, characterized by reduced water absorption, heightened salt uptake (especially sodium, chlorine, and boron), and the generation of reactive oxygen species, induces oxidative stress in plants, severely impacting their growth and overall performance. To enhance plant tolerance to salinity stress, elicitors are employed as a short-term and viable solution to mitigate the adverse effects of stress. Copper, serving as a cofactor and essential element for numerous enzymes involved in photosynthesis and respiration processes, plays a crucial role in sustaining natural plant growth and metabolism. Copper ions function as cofactors in enzymes like superoxide dismutase (Cu/Zn SOD) and polyphenol oxidase, contributing to the removal of reactive oxygen species. However, the absence of this element in plants cultivated in alkaline and saline soils of arid and semi-arid regions can lead to nutritional disorders. In this context, copper nanoparticles emerge as a suitable alternative to chemical fertilizers due to their quicker and more efficient impact. Their use not only mitigates the negative consequences of excessive fertilizer application but also reduces the frequency of application. The Persian leek (Allium ampeloprasum subsp. Persicum) is a valuable edible-medicinal plant native to Iran, belonging to the Amaryllidaceae family. It holds significance in Iran as a key leafy vegetable, valued for its freshness and high processing potential among horticultural plants. Given the nutritional and medicinal importance of Persian leek and the prevalence of salinity stress, this study aims to explore the impact of copper nanoparticle spray in modifying the effects of salinity stress on the morphophysiological and biochemical characteristics of Persian leek.Materials and methodsA factorial experiment was conducted using a completely randomized design with three replications in the research greenhouses of Lorestan University's Faculty of Agriculture. The experimental conditions included daytime temperatures ranging from 20 to 28 °C, nighttime temperatures from 15 to 20 °C, relative humidity set at 60-70%, and a light intensity of 400-500 µmol m-2 s-1. The first factor involved foliar spraying of copper nanoparticles at control levels (zero), 150, and 300 mg/liter, while the second factor comprised salinity stress at control levels (zero), 50, 100, and 150 mM sodium chloride. F1 seeds were obtained from Pakan Bazr Company and planted in 1.5-liter pots, with each pot containing three plants. The copper nanoparticles were applied through foliar spraying twice on the shoot parts at the four-leaf and six-leaf stages. Salinity stress was introduced one week after the foliar application, implemented through irrigation once every three days at a level corresponding to 90% of the field capacity. The soil mixture comprised an equal ratio of agricultural soil, cow manure, and sand, maintaining a clay-sand loam texture. Following three months of applying salt stress, a comprehensive assessment of morphophysiological characteristics was carried out. This included the measurement of plant height, stem and bulb diameter, leaf count, fresh and dry weights of stem, root leaf, root volume and length, shoot/root ratio, dry matter (%), stress tolerance index, relative water content (RWC), electrolyte leakage, malondialdehyde content, photosynthetic pigments, chlorophyll stability index, as well as the activity of peroxidase and ascorbate peroxidase. Results and DiscussionThe results indicated that salinity stress had a detrimental impact on various aspects of plant growth, including a decrease in plant height, stem and bulb diameter, leaf number, and the fresh and dry weights of the stem, bulb, and root. Additionally, there was a reduction in root volume and length, along with decreased levels of photosynthetic pigments. The percentage of electrolyte leakage, malondialdehyde content, and the activity of antioxidant enzymes, namely peroxidase and ascorbate peroxidase, also increased, highlighting the adverse effects of salinity stress on plant development. The decline in plant growth can be attributed to multiple factors, including diminished cell division, ionic imbalance, reduced water absorption, impaired uptake of essential elements, and the impact of toxic ions, particularly sodium and chlorine. Other contributing factors include impaired absorption, regeneration, and metabolism of nitrogen and protein, as well as stomatal closure, collectively resulting in reduced photosynthetic efficiency. Salinity stress further leads to a reduction in soil water potential and an increase in the osmotic pressure of the soil solution. Consequently, the plant requires more energy to absorb water from the soil, leading to increased respiration and alterations in the hormonal balance of plant tissues, ultimately causing a decrease in growth and negative effects on the plant. The application of copper nanoparticles at both concentrations demonstrated positive effects on various growth components, including plant height, stem and bulb diameter, leaf count, and the fresh and dry weights of the stem, bulb, and root, as well as increased root volume and length. Additionally, the use of copper nanoparticles resulted in a decrease in the percentage of electrolyte leakage and malondialdehyde content, coupled with an increase in the concentration of photosynthetic pigments and the activity of antioxidant enzymes, including peroxidase and ascorbate peroxidase. Notably, the concentration of 150 mg/liter exhibited a more pronounced effect in enhancing plant growth, with a diminishing impact observed at higher concentrations. Copper \nanoparticles improve plant growth under stress conditions by influencing the content of cellular antioxidants and modulating the hormonal balance of plant tissues.ConclusionThe findings of this study indicated that increased salinity stress led to higher electrolyte leakage and malondialdehyde content, along with a reduction in RWC and photosynthetic pigments. These changes caused a decline in the morpho-physiological characteristics of Persian leek. However, salinity stress also increased the activity of peroxidase and ascorbate peroxidase enzymes. Foliar application of copper nanoparticles under these conditions had beneficial effects on the plants. Specifically, at a concentration of 150 mg/liter, the negative effects of salinity stress on the morpho-physiological indices of Persian leek were alleviated. This improvement was due to an increase in the activity of antioxidant enzymes, RWC, and the concentration of photosynthetic pigments.
Research Article
Growing vegetables
Fahimeh Yarmohammadi; Alireza Motallebiazar; Samaneh Kazemiani; Mina Amani
Abstract
AbstractIntroduction: Considering the sensitivity of potatoes to viruses, the production of virus-free plants through in vitro cultivation and their propagation leads to a reduction in costs and an increase in yield. One of the effective methods of reducing plant diseases and producing disease-free microtubers ...
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AbstractIntroduction: Considering the sensitivity of potatoes to viruses, the production of virus-free plants through in vitro cultivation and their propagation leads to a reduction in costs and an increase in yield. One of the effective methods of reducing plant diseases and producing disease-free microtubers is the use of in-vitro production methods. Considering the role and importance of macro elements and micro elements in the growth of microtubers, it is possible to change the composition of MS culture medium by changing the concentration of salts of macro elements and micro elements without disturbing the balance of elements. This experiment aims to investigate the effect of different concentrations of macro elements (2 Mac, Mac, ½ Mac) and micro elements (2 Mic, Mic, ½ Mic) of MS culture medium in combination with two concentrations of sucrose (80 and 160 g/liter) was performed on in vitro micronodulation of Agria potato.Materials and Methods: This experiment to investigate the effect of different concentrations of macro elements (2 Mac, Mac, ½ Mac) and micro elements (2Mic, Mic, ½ Mic) of MS culture medium in combination with two concentrations of sucrose (80 and 160 (g/liter) on in vitro microtuberation of Agria potato was carried out as a factorial experiment in the form of a completely randomized design with 3 replications in the plant tissue culture laboratory of the Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz. Lateral buds obtained from in-vitro shoots were used as explants and were cultured under sterile conditions on different culture mediums for the purpose of microtuberation, and the cultures were kept in continuous darkness and at a temperature of 18±2°C were kept in the growth room. During one month, Microtuber initiation rate and after two months, microtuber formation characteristics were measured.Results and Discussion: The results of the analysis of variance showed that the effect of the concentration of micro elements and the interaction effects of micro elements with different concentrations of sucrose and macro elements were significant only in the case of two traits, the percentage and the speed of microtuber initiation, while all microtuber traits productivity was significantly affected by the interaction of micro elements and macro elements. In all culture mediums with 8% sucrose, the initiation percentage of microtubers was 100% and the initiation rate was also maximum. However, the highest percentage of microtuber formation, weight, length, diameter and number of buds on microtuber was obtained in 2Mac culture medium with 16% sucrose. The results showed that the microtuber that had more weight and size had a higher percentage of dormancy and the buds on the microtuber were not able to germinate and produce microtuber during the stages of microtuber formation.Conclusions: In all traits related to microtubers, except for percentage and speed of microtubers initiation, the effects of micro elements, macro elements and sucrose elements were not significant, and this shows that the three investigated factors cannot independently improve microtubers formation is effective in Agria variety. In all traits of micronodulation, the interaction effect of low consumption elements with other two factors was not significant and this shows that the concentration of low consumption elements in Agria variety is not critical for micronodulation. In all culture mediums with 8 % sucrose, the initiation percentage of microtubers was 100 % and the initiation speed was also the maximum, but when double the concentration of macro elements and 16 % sucrose were used, the initiation percentage and the initiation speed of micro-glands in Agria variety showed a significant decrease. The percentage of micro tuber formation, weight, length, diameter and number of buds on the micro tuber in Agria cultivar were significantly affected by the mutual effect of the concentration of macro elements and sucrose, and the 2 Mac culture medium has 16 % sucrose in the first priority and the ½ culture medium Mac with 8 % sucrose in the second priority was better than the other treatments in terms of the investigated traits. In this research, it was found that the produced micro glands with greater weight and size had a higher percentage of dormancy and during the stages of micro glandogenesis, the buds on the microtubers were not able to germinate and produce micro tubers.
Research Article
Medicinal Plants
Mehrab Yadegari
Abstract
IntroductionSatureja bachtiarica is one of the important endemic species and uses in medicinal and food industries. This plant is rich in essential oil, flavonoids, monoterpene glycosides, trypanocidal terpenoids. The main compounds of the essential oil from S.bachtiarica L. collected from natural habitats ...
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IntroductionSatureja bachtiarica is one of the important endemic species and uses in medicinal and food industries. This plant is rich in essential oil, flavonoids, monoterpene glycosides, trypanocidal terpenoids. The main compounds of the essential oil from S.bachtiarica L. collected from natural habitats are alpha-pinene, p-cymene, carvacrol and gamma-terpinene. The environmental factors cause changes in the growth, quantity, and quality of active substances (such as alkaloids, glycosides, steroids and volatile oils) in the medicinal and aromatic plants. Among environmental stresses, water deficit stress plays an important role in the biosynthesis of secondary metabolites in medicinal and aromatic plants in arid and semi-arid regions like Iran. Drought stress has detrimental effects on growth and yield and can upset the balance between the antioxidant defense system and free radicals by stimulating a series of biochemical reactions, where free radicals cause cell damage. In sustainable production systems of medicinal and aromatic plants, stable quantitative and qualitative functions can be achieved under conditions of environmental stress and it is possible to improve the production of secondary metabolites in these plants under adverse environmental conditions. Therefore, this study was done to evaluate the amino acids on the physiological and phytochemical properties of S.bachtiarica to determine and introduce the best amino acids under water stress. Materials and MethodsThis investigation was done from spring (May) 2022 to fall (September) 2023 at the Research Farm (latitude. 32°20′ N, longitude. 50°51′ E, altitude. 2061 m). Based on the Köppen climate classification, the climate of the area of study is classified as cold and semiarid. This study was conducted in a randomized complete block design (RCBD) in split plot layout with three replications. Irrigation regimes (3, 6, 9 day interval) in main plots and foliar application of amino acids (methionine, lysine, phenylalanine and proline) in sub plots were done. In each year, treatments were applied in 3 stage (3 months after sowing, end of vegetation, before flowering, beginning of flowering and early flowering) and harvesting were done in complete flowering. Weed control was done manually during the entire experiment. During the experiment, no fertilizer and no systemic pesticide and herbicide were used. The harvested shoots were transferred to laboratory for extracting and analysis the leaves and stems of treated plants. For assessment of the total chlorophyll content of S.bachtiarica, the leaves were extracted by 80% acetone. The absorbance of the extraction was read at 470, 648, and 664 nm, and the content of total chlorophyll was calculated as mg per g leaf dry weight. Total phenol content was measured by the Folin-Ciocalteu reagent as reported by Singleton and Rossi. The total phenol content was expressed as mg of gallic acid equivalent per g leaf dry weight according to standard curves of gallic acid. The essential oils were extracted by hydro-distillation and analyzed using GC/MS. Results and DiscussionApplied amino acids significantly influenced the morpho-physiological and phytochemical characters of Satureja bachtiarica. In two years, the highest chlorophyll content (1.41-1.54 mg.g-1FW) and total phenol content (2.45-2.72 mg.g-1FW) were obtained in methionine (2.5 g.l-1) and phenylalanine (100 µM) treatments by irrigation regimes 3 day interval. The highest essential oil contents (1.09-1.61 %) were obtained from the plants treated by methionine (2.5 g.l-1) and phenylalanine (100 µM) treatments by irrigation regime 3 day interval. The main chemical compounds of essential oil were P-cymene (17.12-19.45 %), gamma-terpinene (18.14-16.87 %) and Carvacrol (45.12-51.24 %) belong of cyclic monoterpenes. The treatments had significant effectiveness on cyclic monoterpens such as alpha-terpinene, gamma-terpinene, germacren-d, carvacrol, thymol and p-cymene. Increasing of essential oil content/composition by use of amino acids was seen. The main components of essential oil in plants treated by proline lesser than other treated plants. In two years of this research amino acids of methionine and phenylalanine mad the most amounts of carvacrol and essential oil content. The monoterpene alcohols in volatile oils of the treated plants with amino acids elicitors considerably were higher than the sesquiterpenes. According to ratio of monoterpenes alcohol to sesquiterpenes in volatile oils of the treated S.bachtiarica with different elicitors, the foliar application of amino acids can improve the odor quality of S.bachtiarica volatile oil. ConclusionFoliar application of methionine (2.5 g.l-1) and phenylalanine (100 µM) treatments better than other treatments increased the morpho-physiological and essential oil attributes. Environmental condition plays such as drought is a key role in plant growth and biosynthesis of different metabolic compounds. The observed variation in shoot yield and phytochemical content of S.bachtiarica during the studied years may related to differences in photoperiod, temperature, solar radiation, precipitation and occurrence of biotic and abiotic stresses at different growing seasons. Also amino acids had very important effectiveness on content/composition of essential oil of S.bachtiarica. Generally, treats of methionine and phenylalanine can be a good strategy to improve morpho-physiological characters and essential oil quantity and quality of S.bachtiarica in same climate.
Research Article
Postharvest physiology
Vahid Ajami; Seyyed Hossein Nemati
Abstract
Introduction Watermelon Citrullus lanatus (Thunb) is known as a rich source of various vitamins (such as vitamin A) and phytochemical compounds that have high antioxidant activity. Studies show that characteristics such as taste, nutritional value, cost, and convenience in consumption have a direct impact ...
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Introduction Watermelon Citrullus lanatus (Thunb) is known as a rich source of various vitamins (such as vitamin A) and phytochemical compounds that have high antioxidant activity. Studies show that characteristics such as taste, nutritional value, cost, and convenience in consumption have a direct impact on consumer choice, therefore, in recent decades, the desire to consume and buy cut products has been increasing. Also, due to the large size of the watermelon, to avoid waste, this product can be offered sliced. Damage caused by cutting not only increases respiration and ethylene production, but also increases other biochemical reactions that are responsible for changes in color, taste, and aroma, as well as in the texture and quality of nutrition. Also, cutting the products and removing the natural covering of the fruit creates the conditions for increasing the microbial load. In order to increase the natural resistance of fruits and vegetables as well as maintain the sensory and nutritional quality of fresh products, it is recommended to use environmentally friendly technologies such as salicylic acid (SA). Citric acid, like salicylic acid, is considered a safe compound, and as an organic acid, it can be used as an approved food additive.Due to the positive effects of salicylic acid and citric acid on the quality properties of fresh products, no information has been found regarding the post-harvest use of these compounds on sliced watermelon. Therefore, in the present study, our aim was to investigate the effects of citric acid and salicylic acid treatments on sensory properties, quality, microbial load and color changes of cut watermelon fruit during the storage period. Our findings can provide a new strategy for maintaining the quality of sliced watermelon fruit.The microbial load and fruit tissue softening resulting from fruit cutting lead to a decline in quality due to increased fruit respiration and water loss, which are limiting factors for the post-harvest shelf life of cut watermelon fruits. In the present study, the impact of citric acid and salicylic acid on some quality indices of cut watermelon pieces during the storage period was investigated. Materials and Methods The harvested fruits were transported to the laboratory and their external surface was disinfected with sodium hypochlorite (200 μL/L) and the skin of the fruits was removed by a sharp and sterile knife. The harvested fruits were separated from their peels, and then the fruit flesh was cut into 4 cm by 4 cm pieces. The pieces were immersed in salicylic acid solutions (1 or 2 mM) and citric acid solutions (0.5 and 1 mM) for 2 minutes. Immersion in water was also introduced as a control. Subsequently, the fruits were packaged in polyethylene coverings and stored for 14 days at a temperature of 4 degrees Celsius. Results and Discussion Based on the results of variance analysis, immersion of freshly cut watermelon fruits in different concentrations of citric acid and salicylic acid had a significant effect on the firmness of the fruit tissue at the 1% level. The interaction effects of measurement time and immersion in solutions were significant at the 1% probability level. The highest amount of tissue stiffness was related to the treatments of 1 mM citric acid (4.58 newtons) and 2 mM salicylic acid (4.69 newtons), and the lowest value was obtained from the control samples (3.54 newtons). The highest weight loss was related to the control samples and the lowest amount was obtained from 1 mM citric acid and 2 mM salicylic acid.During the maintenance period, the highest and lowest weight loss was obtained from the control and 2 mM salicylic acid treatments, respectively. The highest amount of soluble solids was obtained from the control treatment and the lowest amount was obtained from the 2 mM salicylic acid treatment. The highest amount of microbial load was obtained from the control samples (6.11), and the lowest amount was obtained from the 2 mM salicylic acid treatment (4.02), followed by the 1 mM salicylic acid treatment (4.17). Also, with the passage of storage time, the amount of microbial load increased significantlyThis study was conducted with the aim of investigating the effect of salicylic acid and citric acid on the quality and microbial characteristics of cut watermelon. This experiment included the use of different concentrations of salicylic acid and citric acid to evaluate their effect on quality parameters and microbial load in watermelon slices. Quality characteristics, including color, firmness, sweetness and overall visual appeal, were measured using standard methods. In addition, the microbial load, including both bacterial and fungal populations, was determined to evaluate the antimicrobial potential of the applied acids. The results showed significant effects of salicylic acid and citric acid on increasing some quality traits and reducing microbial contamination in watermelon slices. This research provides valuable insights into the use of salicylic acid and citric acid as potential agents to improve the quality and safety of cut watermelonConclusions The results indicated that salicylic acid at both concentrations (1 or 2 mM) and citric acid at 1 mM led to a significant reduction in microbial load and weight loss. Moreover, the mentioned treatments restrained the increase in soluble solids content resulting from the post-harvest handling of cut watermelon fruits, contributing to the preservation of fruit tissue strength. The results of evaluating color indices and organoleptic properties indicated that salicylic acid treatments at both concentrations (1 or 2 mM) and citric acid at 1 mM preserved the fruit quality to the best extent. And the best treatment included the application of salicylic acid with a concentration of 2 mM. In general, among the treatments used in this experiment, 2 mM salicylic acid yielded the best results in preserving the quality of cut watermelon fruits during cold storage. Subsequently,
Research Article
Ornamental plants
Sahar Mirzaei; Mehrangiz Chehrazi
Abstract
Introduction Narcissus (Daffodil) with the scientific name (Narcissus tazetta) is a perennial bulbous plant from the Amaryllidaceae family (Mozafarian, 1996; Ghahraman & Atar, 2000). Narcissus is one of the most important ornamental bulbous plants that is used as a cut flower and a potted plant. ...
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Introduction Narcissus (Daffodil) with the scientific name (Narcissus tazetta) is a perennial bulbous plant from the Amaryllidaceae family (Mozafarian, 1996; Ghahraman & Atar, 2000). Narcissus is one of the most important ornamental bulbous plants that is used as a cut flower and a potted plant. Our country has a huge source of native daffodils. Iran, with its climatic diversity, vast area of fertile land and abundant solar energy, is one of the centers of The propagation of plant species is important. Today, plant genetic resources are considered as the most valuable and vital resources of any country. Ornamental plants such as narcissus have been important for mankind since ancient times They are valuable for their beauty, but also for their medicinal properties and as a food source (Chehrazi et al., 2008; Farahmand et al., 2007). Considering the importance of the product in the mentioned cases, preserving the valuable native narcissus plant of our country has many economic and social benefits, also to prevent its extinction. Therefore, this project was carried out to collect and evaluate native daffodil genotypes and introduce superior genotypes.Materials and Methods In this research, the bulbs of narcissus native genotypes were collected from natural narcissus fields in different regions of the country (Khuzestan, Mazandaran, Fars, Ilam and Khorasan provinces). Then bulbs were planted in the research field of the Ornamental Plants Research Institute and the morphological and physiological characteristics of the daffodils were evaluated in the field, including the height of the flowering stem, number of leaves, number of flowers, flowering period, chlorophyll and flavonoid content, bulb size, number and fresh and dry weight were measured. Measuring methods are explained below.Flowering branch height: The height of the narcissus flower branch from the point of contact with the soil to the tip of the flower was measured by a ruler in centimeters.Number of leaves: The number of leaves in each narcissus plant was counted.Number of flowers in each branch: The number of flowers in each branch of narcissus was counted.Flowering period: the number of days from the appearance of the first flower to the time the flowers withered was counted.The size of daughter bulbs: The diameter of daughter bulbs in each narcissus plant was measured by calipers in millimeters.Number of daughter bulbs: The number of daughter bulbs in each narcissus plant was counted.Fresh and dry weight of daughter bulbs: The bulbs of each narcissus plant were removed from the soil. After cleaning the onions from the mud, the onions were weighed by an accurate digital scale, which was recorded as fresh weight, in grams. The onions were then placed in the oven for 72 hours and then weighed again by a precision digital balance, which was recorded as dry weight in grams.Amount of chlorophyll and carotenoids was measured by the method of Maxwell & Johnson, 2000 and amount of flavonoids was measured by the method of Chang et al., 2002.Results and Discussion According to the results of this experiment, Mazandaran and Ilam genotypes showed the highest values for morphological indices. By comparing different populations of Narcissus Shahla, it was observed that the population of Mazandaran with (16.38 cm) had the highest height of flowering stem, with (11.9) had the highest number of flowers, with (55.34) had the highest number of leaves, with (47.33 days) had the longest flowering period, with (8.53) had the largest number of girl onions, with (48.39 mm) had the largest size of girl onions, with (15.88 grams) had the highest fresh weight of girl onions, with (10.68 grams) had the highest dry weight of girl onions. Also, Khuzestan and Mazandaran genotypes showed the highest values for physiological indices. It was observed that the population of Khuzestan with (2.229 mg/g) had the highest amount of chlorophyll, with (1.594 mg/g) had the highest amount of carotenoids and with (1.525 mg/ml) had the highest amount of flavonoids.Conclusions Comparisons of morphological and physiological characteristics of native Iranian narcissus in the field and post-harvest period in different populations of native narcissus (Shahla and Porpar) showed that native Iranian narcissus is a plant suitable for planting in green spaces. The planting of these plants in the area of Mahalat has been successful and if cultivated, along with daily care, weeding and regular watering, it is completely suitable for surface production. Planting native narcissus can be recommended for cities with a climate similar to Mahalat. In order to achieve this, we can introduce the native daffodils of Mazandaran and Ilam regions as the best genotypes. Because in terms of morphological indicators, they have the highest stem height, number of flowers, number of leaves. Also, In order to use a flower pot in the home or office environment, the native daffodils of Khuzestan and Mazandaran regions can be introduced as the best genotypes.
Research Article
Pomology
Seyed Mahmud gholami; Hossein Moradi; hossein sadeghi
Abstract
Tarocco cultivar is one of the most popular cultivars of blood orange, which is propagated by grafting on the growing base of citronmelo, but stagnates in spring grafting. Considering the role of cytokinins in the awakening and growth of buds, the present study was conducted in order to investigate the ...
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Tarocco cultivar is one of the most popular cultivars of blood orange, which is propagated by grafting on the growing base of citronmelo, but stagnates in spring grafting. Considering the role of cytokinins in the awakening and growth of buds, the present study was conducted in order to investigate the effect of time (13, 15 and 17 days after grafting) and the concentration of cytokinin compounds (5000 mg/l benzyl adenine, 1000 mg/l kinetin and 50 mg/l thidiazuron) were carried out on the elimination of stagnation and some traits of tarocco spring grafted on citromelo rootstock. The results showed that the time and type of hormone have a significant effect on the elimination of stagnation and the growth of the spring shoots of taraco. So that the application of thidiazuron hormone treatment 13 days after grafting to the elimination of stagnation in more than 90% of grafts. It was also found that thidiazuron and benzyl adenine treatments had better results compared to quintine treatment on bud awakening and early growth of tarocco spring shoots. The treatment of 50 mg/liter of tidiazuron had the highest number of blossomed buds (91.67), leaf area (118.04 cm2) and number of leaves (16.50), especially when applied 13 days after transplanting. In general, according to the obtained results, the use of 50 mg/liter of tidiazuron 13 days after grafting can be considered as a practical and effective strategy to increase bud awakening and the growth and development of tarocco spring shoots on citronmelo for producers seedlings of this cultivar are recommended.Introduction: Bud dormancy is one of the important issues in planting and cultivation of fruit trees that needs to be addressed in many trees such as orange (Citrus sinensis). Bud dormancy involves cessation of horizontal and vertical growth, lack of budbreak, and reduction in plant activity during cold weather. One of the commercial orange cultivars is Tarocco blood orange which compared to older blood cultivars, is larger in size and with lower alternate bearing has higher marketability. The nursery trees of this cultivar are offered on sour orange, citrange, and vigorous rootstock of citrumelo (a hybrid between trifoliate orange and grapefruit) (Talon et al, 2020). One of the major problems of nurserymen in spring grafting of Tarocco cultivar on vigorous citrumelo rootstock is the failure of about 50 percent of buds to break compared to other cultivars on the same rootstock and other similar rootstocks. This unwanted dormancy leads to a one-year delay in the nursery tree production process and unnecessary occupation of nursery space. Given the commercial importance of blood orange and the adverse effects of bud dormancy on yield and fruit lifetime, solutions are used to control and overcome this problem. One of the effective solutions is the application of cytokinins which can stimulate the growth of graft buds (Yadav and Saini, 2018).Materials and Methods:This research was conducted in a citrus nursery at the University of Agricultural Sciences and Natural Resources of Sari, in late May 2022. Citrumelo seedlings were grown in 5/3 liter pots containing a loamy-silt soil mix in the nursery location. Tarocco cultivar buds were prepared from a seven-year-old mother orchard and T-budding was performed in June (during rootstock bark slipping). All hormone treatments were applied after graft union and before bending the branch using a soft brush on the graft buds. The applied treatments included hormone treatment (control, 5000 mg/L benzyladenine, 1000 mg/L kinetin, and 50 mg/L thidiazuron) and treatment time (13, 15, and 17 days after grafting). After two months, some traits related to budbreak and growth of the grafted buds were evaluated.Results and Discussion: The results showed that thidiazuron and benzyladenine treatments had better effects compared to kinetin treatment on spring budbreak and initial growth of Tarocco grafted buds. In a way that 50 mg/L thidiazuron treatment had the highest number of sprouted buds (67.91), largest leaf area (118.04 cm2), highest number of leaves (16.50), especially when applied 13 days after grafting. Also, in leaf size related traits, leaf area indices, graft growth rate as well as chlorophyll and carotenoid content of Tarocco graft leaves were significantly affected by different hormonal treatments and application times, with 50 mg/L thidiazuron being more effective than other treatments. Cytokinins can promote division and expansion of leaf cells and thereby result in increased cell numbers and improvement of different leaf parameters. Also, cytokinins regulate important physiological processes like photosynthesis. Application of these materials provides cell division especially in areas like buds and growth points and also possibly more buds may form on the spring graft by using these treatments during the grafting process which can lead to increased bud break and faster plant growth (Cook and Bahar, 2017). Increasing cytokinin levels can stimulate the photosynthesis process which results in increased food production, leaf growth and ultimately increased leaf area. On the other hand, cytokinins affect plant metabolism and can regulate production and accumulation of different growth factors. This may lead to a better balance in nutrient distribution and metabolic activities which in turn aids leaf area increase (Hodchek et al., 2023). Finally, according to the obtained results, it can be recommended to nurserymen of this cultivar to use 50 mg/L thidiazuron 13 days after grafting as a practical and effective strategy for increasing spring budbreak, growth and development of Tarocco buds grafted on citrumelo.
Research Article
Medicinal Plants
Samira Tandisseh; Ali Reza Astaraei; Amir Lakzian
Abstract
The effect of organic fertilizers application and humic acid foliar sprying on growth characteristics of stevia (Stevia rebaudiana Bertoni) in a saline soilAbstractSaline soils due to lack of organic matter and poor physicochemical properties and subsequent lack of moisture, usually having poor vegetation. ...
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The effect of organic fertilizers application and humic acid foliar sprying on growth characteristics of stevia (Stevia rebaudiana Bertoni) in a saline soilAbstractSaline soils due to lack of organic matter and poor physicochemical properties and subsequent lack of moisture, usually having poor vegetation. The use of organic fertilizers in saline soils, can improve the growth and tolerance of plants under salinity conditions by improving the physical and chemical properties of the soil. For this purpose, this research was carried out with two types of organic fertilizers to modify saline soil properties along with humic acid foliar application as a stimulant for the growth of the Stevia plant. The experimental factors included the first factor: 1) control (without organic fertilizer), 2) vermi compost (20 ton/ha), 3) vermi compost (40 ton/ha), 4) Spent mushroom compost (20 tons/ha), 5) Spent mushroom compost (40 ton/ha), and the second factor was foliar spraying of humic acid at two levels of 0 and 0.1% in a Completely Randomized Design (factorial) with three replications. Photosynthesis rate, leaf area, plant dry weight, pH, salinity, bulk density and soil organic carbon were investigated in order to determine the most effective organic matter treatment under soil salinity stress conditions. Results showed that spent mushroom compost fertilizer (SMC) significantly decreased soil pH, bulk density, but increased electrical conductivity and soil organic carbon. Leaf area was reduced but total phenol, chlorophyll a, b, total and Carotenoid were increased resulting in significant increase in the dry weight of Stevia plants in saline soil. The highest dry weight of the plant was obtained with the application of 40 tons/ha of SMC fertilizer and humic acid foliar application, which was 45.12% more than the control (without fertilizer). Therefore, SMC fertilizer can be a suitable remediator for saline soil, humic acid spray also had a significant positive effect if used along with SMC soil application.Key words: Stevia sweet leaf, plant biostimulants, humic acid, soil salinity Materials and methods:This experiment was conducted in a completely randomized design (factorial) in boxes (dimensions 30x40x30 cm) containing natural saline soil (EC=10.15 dS/m) under natural agronomic conditions in The Ferdowsi University of Mashhad with three replications from July to January 2018. Experimental factors were included the first factor: 1) control (without organic fertilizer), 2) vermicompost 20 tons/ha, 3) vermicompost 40 tons/ha, 4) Spent mushroom compost 20 tons/ha, 5) Spent mushroom compost 40 tons per hectare and the second factor: foliar spraying of humic acid at two levels of zero and 0.1%.A soil sample was collected from Kushk region of Neyshabur city) with desired salinity (ECe=10.15 dS/m) and some physical and chemical properties of soil and organic fertilizers were measured before the experiment. Organic fertilizers after mixing with the experimental soil, were added to the plastic boxes according to the amount of each treatment. 2 seedlings were planted in each plastic box at a distance of 20 cm. Irrigation was done with tap water daily and one month after the adaptation of the stevia plant to the climatic conditions of Mashhad, humic acid foliar spraying was done in three stages with 20 days intervals.After plant physiological stge some parameters including plant dry weight, leaf area, total plant phenol, amount of chlorophyll a, b, carotenoid, total plant chlorophyll, and soil organic carbon, bulk density, salinity, and soil pH , were determined after plant harvest. The dry weight of the stevia plant was obtained by weighing the plants after drying in an oven for 48 hours at 70°C. Statistical analysis of data was performed by JMP software version 0.8, drawing graphs using Excel software and comparing average data using LSD test at significance levels 1 and 5 percent.Conclusion:According to the results of this research, the application of SMC fertilizer with and without foliar application of humic acid was able to significantly increase the dry weight of the stevia plant compared to the control (without fertilizer) through modifying the physical and chemical properties of saline soil (pH, OC, bulk density) and some phytochemical properties of the plant (Photosynthetic pigments and total phenol). Application of VC fertilizer was not successful. Even though humic acid foliar application increased the total plant phenol, it could not improve the growth of the plant in saline soil alone, but it could increase the dry weight of the plant only with the application of SMC fertilizer. Thus, the highest dry weight of stevia plant was obtained with the application of 40 ton/ha of SMC fertilizer and humic acid spraying.Therefore, it is suggested that in order to achieve proper performance (≥80%) and improvement in the physiological and morphological characteristics of the stevia plant in very saline soil (ECe≥10 dS/m), it is necessary to use 40 tons/ha or more of mushroom compost waste. It should be used in saline soil three to six months before cultivation and 0.1% humic acid foliar spraying should be done three times with an interval of 20 days.
Research Article
Pomology
Saeid Fatahi Siahkamary; Vali Rabiei; Mahmood Shoor; silvana nicola
Abstract
The Lycium genus of the Solanaceae family has excellent nutritional and medicinal value. Two species of Lycium barbarum L. and Lycium chinense Mill. It is often called wolfberry or goji berry. The use of amino acids for horticultural crops is common worldwide, and many chemicals used as biostimulants ...
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The Lycium genus of the Solanaceae family has excellent nutritional and medicinal value. Two species of Lycium barbarum L. and Lycium chinense Mill. It is often called wolfberry or goji berry. The use of amino acids for horticultural crops is common worldwide, and many chemicals used as biostimulants are mixtures of amino acids. The effect of amino acids on plants seems to depend on the type of amino acid supplied and the type of plant. Selenium (Se) is an important component of selenoproteins and seleno-amino acids. Therefore, it has played many roles in the growth and function of living cells and important biological functions in animals and humans. Also Se is very similar in properties to sulfur and can act as S in biochemical systems. Biological fertilizers are fertile materials that contain one or more beneficial soil organisms within a suitable carrier. Overall, these fertilizers contain different types of microorganisms that can convert nutrients from unavailable form to available form through a biological process. The application of supernitroxin fertilizer by stimulating the synthesis of plant hormones increased the growth indicators of sesame varieties.This experiment was conducted in the Research farm University of Mashhad, during 2021 and 2022 years. In early May, goji berry seedlings were planted in the field to check the effect of the L-phenylalanine (Phe), sodium selenate (Se), and Nitroxine were applied before harvesting and foliar spraying on the Goji berry plant during the growth stages. To conduct experiment, two-year-old seedlings of Goji berry cultivar GB1 were obtained from Mashhad Seedling Company located in Razavi Khorasan province, and after the seedlings were transferred to ground and established, initial foliar spraying was done. In order to evaluate the effect of L-phenylalanine, selenium, and nitroxine treatments, experiment were conducted as a randomized complete block design in 5 replications which factors include the amino acid L-phenylalanine (Phe: 0.5, 1, and 1.5 mM), sodium selenate (Se: 0.25, 0.5, and 1 mg.L-1) and nitroxin biological fertilizer (170, 330, and 500 μl/L) at three levels and distilled water was applied as a control. One pot was considered for each repetition and a total of 10 pots were considered along with the control. Plants were sprayed every 15 days after establishment. After the three stages of foliar spraying, the content of phenol and flavonoid content and antioxidant activity, PAL and anthocyanin were measured.The results showed that the treatments used in this experiment had a significant effect on the physiological and chemical characteristics of the goji berry plant. The results showed that the highest amount of titratable acidity (0.896%) was obtained in samples treated with nitroxine at a concentration of 333 microliters. The highest amount (18.56 mg/g) of this index was obtained in fruits treated with phenylalanine at a concentration of 1.5 mM. The highest amount of this index was obtained in the fruits treated with phenylalanine at a concentration of 1 mM, which was 61.98% higher than the amount of flavonoid recorded in goji berries under control conditions. Also, the results showed that despite the decrease in the activity of PAL enzyme during the increase of selenium concentration, the activity of this enzyme was 13.66% higher than the activity of PAL enzyme under the condition of using selenium at a concentration of 1 mg/liter. The increase in the functioning of the antioxidant system is determined by the total antioxidant, which is controlled by the content of low-molecular components and the activity of antioxidant enzymes. Compounds such as ascorbic acid, glutathione, tocopherol, carotenoids, anthocyanins, endogenous metal chelators, TPC, TFC and alkaloids are low molecular weight antioxidants. Also nitroxin, supernitroplus, phosphate and mycorrhizal fertilizers. Nitroxin binds N in the atmosphere and balances the absorption of nutrient element in the plant. Nitroxin is responsible for the secretion of amino acids, antibiotics, hydrogen cyanide and siderophores, which promote the growth and development of plant roots and shoots, protect the roots from pathogens, thereby increasing yield. A sufficient supply of phenylalanine through the use of exogenous Phe or endogenous Phe provided by the shikimic acid pathway may be required to stimulate the activity of the phenylpropanoid pathway, as shown by the high PAL activity responsible for the accumulation of phenols, flavocyanins and anthocyanins. This leads to the need for ROS accumulation. Anthocyanins are groups of flavonoids found in vacuoles epidermal and mesophyll cells of plants. Anthocyanins can protect chlorophyll from light oxidation and, compared to other components, is a better indicator of plant oxidative stress caused by external factors that accumulate in plantsThe present study was conducted with the aim of studying the effect of L-phenylalanine, selenium and nitroxin biofertilizer on improving the vegetative growth, yield and secondary metabolites of gojiberry during two crop years. To conduct the experiment, two-year-old seedlings of Goji berry variety GB1 were obtained from Mashhad Seedling Company located in Razavi Khorasan province. The results showed that the use of L-phenylalanine amino acid, selenium and nitroxin biofertilizer had significant effects on the investigated traits during the experiment. So that the use of nitroxin at a concentration of 166 microliters increased the amount of soluble solids and at a concentration of 333 the titratable acidity compared to the control samples. PAL enzyme was obtained in the conditions of using phenylalanine at a concentration of 1.5 mM, which was 52.17% more than the level of PAL enzyme activity recorded in control conditions.
Research Article
Medicinal Plants
Saeideh Mohtashami; Hossein Gholami; Askar Ghani; Mojahed Kamalizadeh
Abstract
Introduction: Optimum nutrition of plants and reducing the effect of lack of important nutrients is one of the most effective factors on the production of all plants; so, it is one of the main pillars in improving the quantitative and qualitative attributes of the product. In order to achieve optimal ...
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Introduction: Optimum nutrition of plants and reducing the effect of lack of important nutrients is one of the most effective factors on the production of all plants; so, it is one of the main pillars in improving the quantitative and qualitative attributes of the product. In order to achieve optimal yield with proper nutrition of the plant, each element must be provided to the plant sufficiently, and there must also be a balance between the absorbable amounts of elements in the soil. Securigera securidaca is one of the medicinal plants belonging to the Fabaceae family. There are many records about the traditional use of this plant and its seeds. Its seeds have antiparasitic, anticonvulsant, antihypertensive, antiulcer, analgesic, antioxidant, antiviral, antitumor and hypoglycemic effects have been reviewed. Considering the climatic conditions of Iran and its high potential in the production of medicinal plants and its economic justification,; it is very necessary to pay attention to the nutrition of medicinal plants due to their different growth conditions with other crops and its effect on their growth and effective substances.Materials and methods: In this research, in order to evaluate the response of the Hatchet vetch plant (Securigera securidaca) to different levels of phosphorus and zinc foliar application, a factorial experiment was conducted in a randomized complete block design (RCBD) with two factors and three replications. The treatments include four levels of phosphorus (0, 50, 100 and 150 kg/h) and three levels of zinc foliar application during the flowering period (no foliar application as a control, foliar application: each two weeks once and once a week). Zinc foliar spraying was done with a concentration of 4 g per liter during before flowering to the formation of the pods (about one month), which was done twice and four times for the two-weekly and once-a-week treatments, respectively. This research was carried out in Mohammad Abad area of Jahrom city located in Fars province. The most important indicators of growth and yield were measured including: shoot number, length of main stem, number of pods per plant, number of seeds per pod, pod length and seed yield. Also, the most important biochemical characteristics of seed extract (flavon and flavonol, total flavonoid, total phenolic compounds, tannin content and antioxidant activity), oil percentage and seed oil yield were also measured.Results and discussion: Based on the results obtained from the variance analysis of the data, the effect of phosphorus and zinc on all measured traits was significant except for the number of seeds in pods and pod length. The results showed that the application of phosphorus alone was more effective than the combined application of phosphorus and zinc in increasing the number of stems, main stem length, seed yield, oil yield, the amount of flavonoids and antioxidant activity of Hatchet vetch plant seeds. The application of 50 kg of phosphorus increased the number of stems, the length of the main stem, the number of pods per plant, the yield of seeds, amount of phenolic compounds and oil yield of seed. While the use of 100 kg of phosphorus without the use of zinc was more effective in increasing the amount of flavonoid and antioxidant activity. However, the application of zinc was effective in increasing phenolic compounds, tannin content, oil percentage and seed yield. The interaction effect of the treatments showed that the highest seed yield (61.03 g/m2) was related to 50 kg of phosphorus without zinc foliar spraying, while the lowest amount (32.01 g) was observed in 50 kg of phosphorus and zinc solution once a week treatment. The highest amount of total flavonoid (3.58 mg/g dry weight) was found in the treatment of 100 kg of phosphorus without the application of zinc. While the lowest amount (1.11 and 1.24 mg) was observed in treatments without phosphorus (spraying zinc solution once every two weeks and once a week), respectively. In the treatments of 100 and 150 kg of phosphorus, increasing the frequency of zinc foliar spraying has reduced the amount of phenolic compounds in the seeds of this plant. Although this decrease is not significant statistically. Also, in the control, 50 and 150 kg of phosphorus treatments, increasing the frequency of zinc foliar spraying had no effect on the change of its antioxidant activity, while in the treatment of 100 kg of phosphorus fertilizer, increasing the frequency of zinc foliar spraying decreased the antioxidant activity of the seed extract of this plant.Conclusion: In general, according to the conditions in this research, despite the differences that were seen in the measured traits in response to the amounts of phosphorus and zinc, the concentration of 50 kg of phosphorus and foliar spraying of zinc once every two weeks, in most of the traits were satisfactory compared to other treatments.
Research Article
Medicinal Plants
Mansoore Kermani; Shahram Amirmoradi
Abstract
IntroductionDrought stress is one of the most important and most common limiting factors for plant growth in arid and semiarid regions. Asparagus (Asparagus afficinalis) is the most important species of asparagus and the fresh stem of this plant is harvested as its edible part and used as a vegetable ...
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IntroductionDrought stress is one of the most important and most common limiting factors for plant growth in arid and semiarid regions. Asparagus (Asparagus afficinalis) is the most important species of asparagus and the fresh stem of this plant is harvested as its edible part and used as a vegetable and medicine. Asparagus is very rich in vitamins, flavonoids, saponins, and glutathione, which have anti-cancer properties, protect nerve cells, and protect the heart. Asparagus is a herbaceous, perennial plant growing to 100–150 cm tall, with two types of stems. One of the stems called spear, which is edible and has no leaves. The second, called fern which has needle leaves and is non-edible. This plant has thick and fleshy roots called rhizome (crown), which is resistant to winter cold and can remain in the ground for up to 25 years and give economic crops for up to 10-15 years. According to some studies, commercial cultivars of asparagus are sensitive to abiotic stresses such as drought stress. Drought stress during the growth of fern can reduce the production of stored carbohydrates in the crown roots and lead to a decrease in yield in the next season. Also, drought stress increases the fiber and reduces the quality of asparagus. Mulch is any material that is spread or laid over the surface of the soil and used for reasons such as saving water consumption by reducing evaporation from the soil surface, controlling soil erosion, adjusting soil temperature, controlling the growth of weeds, and improving the absorption of soil nutrients. Several researches have reported that the use of mulch is effective in increasing the resistance of plants to drought stress and also reducing the negative effects of drought stress on yield and its components.Materials and MethodsThis research was conducted as a factorial experiment based on a randomized complete block design in three replications in the crop year of 2021-2022 in a two-year asparagus field near Mashhad city. The experimental factors were the types of mulch (barley straw, asparagus straw and plastic) and drought stress levels including irrigation after 50 mm (no stress), 70 mm (mild stress), 100 mm (moderate stress) and 150 mm (severe stress) evaporation from pan class A. Planting of transplants in the field was done manually on April 9 2021. Each experimental plot with dimensions of 4 x 10 meters included 4 rows with a distance of 100 cm and a distance of 50 cm between plants. Weeding was done manually. Fertilization was done according to the soil analysis and the recommendations of the soil science laboratory. The date of applying water stress was from April 4 to September 1 2022. The average of traits recorded in the first harvest (April 21) and the second harvest (September 6) were used for variance analysis. Data analysis was done using MSTAT-C software and mean comparisons were done using LSD method at 5% probability level. The graphs were drawn using Excel software.Results and DiscussionThe results showed that fresh stem yield, number of harvestable stems, stem length, stem diameter, stem fresh and dry weight, and total leaf chlorophyll content decreased and leaf proline content increased significantly at different levels of water stress. The content of leaf carotenoids also decreased significantly compared to the control only in severe water stress. The use of mulch in non-stressed conditions caused a significant increase in all studied traits except proline, so that asparagus straw with a 76% increase in stem yield was in the first place, and barley straw and plastic mulch were in the second and third places. Also, mulching reduced the negative effects of water stress on all traits, so that the non-use of mulch in mild, moderate and severe stress caused a 21%, 36% and 46% reduction in shoot yield, respectively, and the use of asparagus straw caused 10 %, 13% and 16% decrease in the yield of stems.ConclusionWater stress, as the most important factor of physiological limitation in plants, negatively affects growth, development and yield of plants. The use of plant residue mulches, especially asparagus straw, between the rows of asparagus cultivation can increase the yield in conditions without drought stress, and in drought stress conditions. the yield can be protected from the negative effects of stress to a large extent.
Research Article
Ornamental plants
rasoul abaszadeh faruji; abdollah hatam zadeh; Ahmad Sharifi; Mahdiyeh Kharrazi
Abstract
IntroductionLight is recognized as a vital factor for plant growth and development. Plants convert light energy into chemical energy through photosynthesis, which is then used for growth and development. Quality, intensity, and photoperiod are among the factors that directly affect plant growth and development ...
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IntroductionLight is recognized as a vital factor for plant growth and development. Plants convert light energy into chemical energy through photosynthesis, which is then used for growth and development. Quality, intensity, and photoperiod are among the factors that directly affect plant growth and development processes. In recent years, Light-Emitting Diode (LED) technology has gained significant popularity in agriculture due to its numerous advantages over traditional light sources. These advantages include the ability to produce various light spectra, low energy consumption, long lifespan, and reduced heat emission. These characteristics have made LEDs an ideal light source for cultivating plants in controlled environments such as greenhouses and growth chambers. The primary objective of this study was to investigate the effects of different LED light qualities on the morphological, physiological, and germination traits of marigold (Tagetes erecta) seeds. Given the importance of light in plant growth and the benefits of LED technology, this study can provide valuable insights for improving crop cultivation and production.Materials and MethodsThis experiment was conducted in the Biotechnology Laboratory of Horticultural Plants in the Academic Center for Education, Culture and Research of Khorasan Razavi. F1 hybrid seeds were used in this study. The experimental treatments consisted of five light qualities: white light (100%), blue light (100%), red light (100%), 30% blue light + 70% red light, and 70% blue light + 30% red light. All treatments were subjected to a 16-hour light and 8-hour dark photoperiod using LED grow lights. The photosynthetic photon flux density (PPFD) was maintained at a constant 100 μmol.m⁻².s⁻¹ for all light treatments. Seed germination parameters (Seed Germination percentage, Mean germination Time, Germination rate, Radicle length, Plumule length, lateral roots number, Plumule fresh weight, Radicle fresh weight, Plumule dry weight and Radicle dry) were initially measured in Petri dishes under the growth panels. Subsequently, seeds were sown and grown under the growth panels, and physiological and morphological parameters including plant height, first internode length, stem diameter, node number, leaf area, leaf length, leaf number, lateral shoot number, shoot fresh weight, shoot dry weight, shoot fresh/ dry weight ratio, root fresh weight, root dry weight, root fresh/ dry weight ratio, dry matter, root length, electrolyte leakage, relative leaf water content and chlorophyll content were measured every 15 days for a total of four measurements. Results and DiscussionSeed germination indices showed that the lowest mean germination time, highest germination rate, longest radicle length, hypocotyl length, number of lateral roots, and fresh weight of radicles were observed under 100% red light treatment, followed by the 70% red + 30% blue light treatment. The application of 100% red light improved germination rate, radicle length, hypocotyl length, number of lateral roots, and fresh weight of radicles by approximately 14%, 29%, 48%, 100%, and 67%, respectively, compared to the control. Plants grown under 100% red light exhibited the greatest plant height at both the beginning and end of the growth period. At the end of the growth stage (75 days after sowing), plants under 100% red light showed increases of approximately 37%, 6%, 33%, and 31% in stem diameter, length of the largest compound leaf, number of leaves, and number of branches, respectively, compared to the white light treatment at the same growth stage. Additionally, the fresh and dry weights of plants increased by approximately 56% and 9%, respectively, compared to the control at the same growth stage. A study of the fresh and dry weights of roots showed that the application of 100% red light increased these two indices by nearly 3 times compared to the control. The lowest fresh and dry root weights were observed under 100% blue light treatment, followed by the 30% red + 70% blue light treatment. Furthermore, plants grown under 100% red light exhibited higher relative water content and lower electrolyte leakage in leaves compared to plants grown under other light treatments.ConclusionsThe research findings indicated that the application of light-emitting diodes (LEDs) with various light qualities enhanced the growth conditions of Tagetes erecta Antigua orange. Comparisons among the light treatments showed that the use of 100% red light resulted in increased germination percentage and rate in marigold seeds. Furthermore, the application of red light under controlled conditions led to an increase in plant growth indices compared to other experimental treatments. Therefore, the use of red light at different growth stages of marigold under controlled conditions is recommended
Research Article
Pomology
Soraya Karami; Sakine Faraji
Abstract
IntroductionMineral nutrients (macro and micro) and polyphenolic compounds are natural components of many fruits and play an important role in maintaining the quality and nutritional value of the fruit. Therefore, optimal management of plant nutrition in order to increase the quantity and quality of ...
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IntroductionMineral nutrients (macro and micro) and polyphenolic compounds are natural components of many fruits and play an important role in maintaining the quality and nutritional value of the fruit. Therefore, optimal management of plant nutrition in order to increase the quantity and quality of the product and improve the synthesis of secondary metabolites, especially during fruit growth, is necessary and unavoidable. Nevertheless, in the last decade, the occurrence of pomegranate aril paleness has been reported as a new and pervasive factor in reducing the quality of pomegranate fruit in many countries, including Iran. Therefore, the present research was conducted with the aim of comparing mineral (concentration of iron, zinc and calcium) and biochemical (anthocyanin content) in pomegranates affected by aril paleness and healthy pomegranates of "Malas Saveh" cultivar (year 2022) and then investigating the effect of foliar application of the mineral elements mentioned on the incidence and severity of the aril paleness complication (year 2023). Materials and MethodsThis study was carried out during two years (2022 and 2023) and two independent trials. First, based on the introduced factors affecting the occurrence of pomegranate paleness (temperature, irrigation water and soil salinity), two orchards with medium and high percentage of pomegranate aril paleness (orchard number 9 and 17, respectively) were selected based on the results of Faraji & Karami (2024a). The first trial in 2022 (orchard No. 9): At harvest and after splitting fruits, twenty healthy fruits and affected fruits by the aril paleness disorder were randomly selected and were used for determination of mineral nutrient (Fe, Zn and Ca) and anthocyanin content in the laboratory. The second trial in 2023 (orchard No. 17): In the first phase, 70 trees were selected and labeled, then at the end of September (2022), percent and severity aril paleness of each tree was calculated. In the second phase (2023), based on the results of the first year, 54 trees as experimental unit (with aril paleness percentage>85 and paleness severity of high/very high) were selected from previous trees. Afterwards, a factorial experiment (3×3×2) in a randomized complete block design with three replications and two stages (the beginning of fruit set and the beginning of fruit ripening) were implemented. Experimental treatments included foliar spraying with three concentrations of iron sulfate (0, 2 and 4 per thousand respectively F0, F2 and F4), three concentrations of zinc sulfate (0, 3 and 6 per thousand respectively Zn0, Zn3 and Zn6) and two concentrations of calcium chloride (0 and 4 per thousand respectively Ca0 and Ca4). Then at harvest, the percentage and severity of aril paleness each treatment was calculated. Results and DiscussionData analysis using the independent t-test(α=0.05, df=38) for the first trial (2022) showed that, the nutritional value of the affected fruit by aril paleness is anticipated to be far less than that of the healthy fruit; so that except for the Zn concentration, the concentration of Fe, Ca and anthocyanin content in affected fruits were lower than in healthy fruits. Besides, the results of variance analysis for the second trial (2023) showed that foliar spraying with iron sulfate, zinc sulfate and calcium chloride individually or in combination was effective in reducing the occurrence and severity of aril paleness. Comparison the means percentage of aril paleness between the two groups (before and after foliar spraying regardless of the type of treatment combination) based on the dependent t-test (α=0.05, df=16) also showed that foliar spraying was effective in reducing the percentage of aril paleness. On the other hand, the effect of the participation of calcium element in reducing the incidence and severity of aril paleness was significant. In the absence of calcium element, the incidence of paleness was recorded in the range of 60.17-75.00%, and with the inclusion of calcium in the treatment compounds, the percentage of paleness was recorded in the range of 21.83-0.53%. Also, the mean comparison of the double interaction and main effects of elements indicated the synergistic effect of calcium element with Fe and Zn element in reducing the aril paleness disorder. So that, the combination of Fe-Ca and Zn-Ca has been more successful in reducing the aril paleness disorder than the pure application of each element of iron, zinc and calcium.ConclusionOverall, the simultaneous application of Zn-Fe in combination with Ca was more effective in reducing of the mentioned traits than other treatments. Therefore, in order to simultaneously reduce the occurrence and severity of aril paleness, spraying with Fe4Zn6Ca4 and Fe4Zn3Ca4 is recommended in two stages of pomegranate fruit development, including the beginning of fruit set and the beginning of fruit ripening.
Research Article
Growing vegetables
Mohsen Mohammad Rezaei; Moazzam Hassanpour Asil; Jamal-Ali Olfati; Mohammad Mehdi Gheisari
Abstract
IntroductionThe Persian Shallot (Allium hirtifolium Boiss) is a valuable medicinal plant native to Iran, known for its significant economic value and medicinal properties. Its rich phytochemical composition makes it useful in treating various conditions such as rheumatism, stomach ulcers, and microbial ...
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IntroductionThe Persian Shallot (Allium hirtifolium Boiss) is a valuable medicinal plant native to Iran, known for its significant economic value and medicinal properties. Its rich phytochemical composition makes it useful in treating various conditions such as rheumatism, stomach ulcers, and microbial infections, while also showing potential as an aquaculture regulator. However, the overexploitation of natural habitats poses a threat to its biodiversity and survival. Sustainable practices in collection and cultivation are essential to address the increasing global demand while preserving genetic and chemical diversity. Iran's favorable climate and plant diversity position it well for the production and export of high-quality medicinal plants, including the Persian Shallot. Research on the nutritional value of native plants can further contribute to their recognition and utilization.Material and MethodsThis study was carried out in six habitats of Allium hirtifolium Boiss in Isfahan province in 2022. The research aimed to assess the morphophysiological and phytochemical characteristics of Persian shallot plants by collecting samples from six natural habitats at different altitudes. Each habitat yielded 50 samples during the growing season, which were then evaluated for traits such as number of leaf, leaf area surface, fresh and dry weight of onion of Persian shallot. Phytochemical analysis involved grinding fresh plant leaves with acetone, followed by spectrophotometric readings to determine chlorophyll, carotenoid concentration, and antioxidant activity. Data analysis was carried out using variance analysis to compare means and cluster analysis to group habitats based on their traits. The study employed SAS, SPSS and Excel software for statistical analysis and visualization. Phytochemical analysis involved grinding fresh plant leaves with acetone, followed by spectrophotometric readings to determine chlorophyll, carotenoid concentration, and antioxidant activity. Data analysis was carried out using variance analysis to compare means and cluster analysis to group habitats based on their traits. The study employed SAS, SPSS and Excel software for statistical analysis and visualization. The study employed SAS, SPSS and Excel software for statistical analysis and visualization. The study employed SAS, SPSS and Excel software for statistical analysis and visualization.Results and DiscussionThe research revealed significant diversity among Iranian shallot plants collected from different habitats, indicating the presence of substantial biodiversity due to environmental factors and genetics. The study found variations in morphological and phytochemical traits, such as fresh and dry weight, leaf area, chlorophyll and carotenoid content, and antioxidant activity among different plant stands. These differences were linked to factors like altitude, temperature, and soil conditions. Altitude was particularly influential, with higher altitudes leading to increased leaf surface and chlorophyll content. Furthermore, cluster analysis showed distinct groupings among the populations based on their traits, highlighting the impact of genetic factors and climatic conditions on the plants. The findings underscore the importance of understanding plant responses to natural growth conditions and environmental factors for breeding programs. Altitude gradients were identified as crucial in influencing plant characteristics and species distribution.Altitude was particularly influential, with higher altitudes leading to increased leaf surface and chlorophyll content. Furthermore, cluster analysis showed distinct groupings among the populations based on their traits, highlighting the impact of genetic factors and climatic conditions on the plants. The findings underscore the importance of understanding plant responses to natural growth conditions and environmental factors for breeding programs. Altitude gradients were identified as crucial in influencing plant characteristics and species distribution.Altitude was particularly influential, with higher altitudes leading to increased leaf surface and chlorophyll content. Furthermore, cluster analysis showed distinct groupings among the populations based on their traits, highlighting the impact of genetic factors and climatic conditions on the plants. The findings underscore the importance of understanding plant responses to natural growth conditions and environmental factors for breeding programs. Altitude gradients were identified as crucial in influencing plant characteristics and species distribution.ConclusionThe study confirms the presence of biodiversity among shallot populations in six habitats, indicating the potential for selecting suitable populations and genotypes. Results suggest that altitude variation has influenced genetic diversity and phytochemical composition, highlighting the impact of climatic and geographical factors on population diversity. climatic and geographical factors on population diversity. climatic and geographical factors on population diversity. climatic and geographical factors on population diversity. Further research is needed to identify specific factors contributing to diversity in Isfahan province and molecular markers can enhance understanding of population diversity.
Research Article
Medicinal Plants
Faeze Rezvaninia; Sahar Zamani; Mohammad-Taghi Ebadi
Abstract
AbstractIntroductionThe increasing use of medicinal, aromatic plants and spices has increased the economic production, value and trade of these plants. Ginger (Zingiber officinale Roscoe.) is a perennial rhizome plant which is considered as the most important spice in the global trade. Ginger rhizome ...
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AbstractIntroductionThe increasing use of medicinal, aromatic plants and spices has increased the economic production, value and trade of these plants. Ginger (Zingiber officinale Roscoe.) is a perennial rhizome plant which is considered as the most important spice in the global trade. Ginger rhizome has bioactive compounds such as phenolic compounds and essential oil. Essential oil (including zingiberene, α and β-pinene, camphene etc.) is responsible for the aroma of ginger. Important phenolic compounds of ginger include Gingerol, Shogaol, Paradol, and Zingerone, which are responsible for the spicy taste of ginger. Ginger has important effects such as anti-inflammatory, antioxidant, antimicrobial, antilipemic, anti-obesity, anti-diabetes, anti-nausea, and liver protective activities, improving digestive problems, etc. In addition to medicine, ginger rhizome is widely used as a spice and flavoring in foods, drinks, etc., and is also defined as a dietary supplement by the World Health Organization (WHO) and the Food and Agriculture Organization (FAO). In this study, while examining and analyzing the world market of this valuable spice, Iran's production potentials were examined to supply internal needs and enter to the export section.Material and Method In this research, the data on the production and trade of ginger was evaluated using the codes determined in the Harmonized System (HS codes 091010, 091011 and 091012). The area harvest, production amount, yield and the top producing countries were extracted from the statistics section of the Food and Agriculture Organization of the United Nations (FAO). The export and import trade data of ginger in Iran and the world, the top exporting and importing countries during 2010-2022 years were evaluated using the World Trade Center data.Results and Discussion According to the obtained assessments, the total production of ginger in the world has increased from 1.718.571 tons in 2010 with an increase of 183% to 4.874.216 tons in 2022, and India, Nigeria, China, Nepal, and Indonesia are among the main ginger producer countries in the world during these years. Also, the investigation of the global trade of ginger showed that the export of this product increased from about 655.9 million dollars in 2010 with a growth of 37% to 900.5 million dollars in 2022, and the import value also increased by 97%, from 568.7 million dollars in 2010 to 1.125 billion dollars, which indicates an increase in the global trade of this product. China, Netherlands, India, Peru, and Thailand were the top exporters while America, Japan, Netherlands, Germany, and Pakistan as the top importers of ginger. The analysis of Iran statistics showed that the total export value of ginger was 15.000 dollars in 2022, while the amount of import reached 36.6 million dollars from 1.35 million dollars in 2010, which shows an increase of 4.7 folds the import volume of this product. Iraq and Azerbaijan were the destinations of ginger exported from Iran, and the origins of ginger imported to Iran were China, United Arab Emirates, and India. In Iran, Tehran province was the only ginger-producer province in the country, which produced 23 tons of ginger with 0.3 ha of area in greenhouse conditions. Price fluctuations of ginger produced in countries such as the USA, China, Brazil and Peru and offered in the USA market in 2022 were investigated. The price of ginger varies throughout the year due to factors such as supply, demand, weather conditions and market dynamics. The highest ginger price ($9.19) was recorded for American ginger in the USA market from August 19 to September 14, and it still had a high price in the cold season. As mentioned, ginger is one of the most important spices in global trade and has a high value in global export and import. In Iran, production less than the internal demand has caused a significant increase in imports compared to exports. Therefore, according to the evaluation and success of the cultivation of similar products in the country, the existence of wide areas harvested and favorable weather conditions, especially in provinces such as Sistan & Baluchestan, and Hormozgan, it is suggested to pay attention to the cultivation of ginger to supply internal markets and achieve extensive exports. Also, according to the area under greenhouses cultivation, which is 24,000 hectares, taking into account the average production of 10 tons of ginger per hectare, if only 10% of the existing greenhouse cultivation is dedicated to ginger production, it is possible to produce 24,000 tons of fresh ginger, which will fully meet domestic needs and provide the export.
Research Article
Growing vegetables
Fahimeh Ghaemizadeh; Farshad Dashti
Abstract
Introduction At present, garlic production is completely dependent on asexual propagation, but a wide diversity of bolting and scape formation is observed in garlic. Based on their ability to produce flowering stem (scape), garlic clones are classified into non-bolting, semi-bolting, and bolting clones. ...
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Introduction At present, garlic production is completely dependent on asexual propagation, but a wide diversity of bolting and scape formation is observed in garlic. Based on their ability to produce flowering stem (scape), garlic clones are classified into non-bolting, semi-bolting, and bolting clones. In non-bolting clones, scape is not formed or abort at early stages. In semi-bolting clones, cessation of scape development often results in the formation of very short scape between the leaves and development of the elongated leaf-like bracts in the center of the inflorescence. In bolting clones after exposure to low temperature during autumn and winter (vernalization) and long day during spring, long and thick scape is formed which contains inflorescence with flowers. Transition from vegetative to reproductive phase and formation of scape, inflorescences and flowers in plants includes a series of continuous stages which control by several gene groups. Vernalization reduces the expression of the flowering inhibitors like FLOWERING LOCUC C (FLC), resulting in increased flowering integrators expression like SUPPRESSOR OF OVER EXPRESSON OF CONSTANT 1 (SOC1), APETALA 1 (AP1) and APETALA 1 (AP2). So, a correct understanding of the vernalization control genes expression pattern will improve garlic flowering and breeding programs. The aim of this study is to investigate the relative expression of AsFLC, AsSOC1, AsAP1 and AsAP2 before and after vernalization in Iranian bolting, semi-bolting and non-bolting garlic clones.Material methodsIn this study, three garlic clones including, bolting (Mazand Zabol), semi-bolting (Langrud), and non-bolting (Hamedan) garlic clones were selected from vegetable collection of Bu-Ali Sina university (Hamedan, Iran). At first, RNA extracted from meristems of three clones monthly, from 4 to 20 weeks after planting (for AsFLC, AsSOC1, AsAP1 and AsAP2 expression analysis) and from inflorescence of semi-bolting and bolting clones at 24 weeks after planting (for AsSOC1, AsAP1 and AsAP2 expression analysis) at 2 biological replicates. Then, cDNA synthesized using Oligo d(T) primer and relative expression pattern of the mentioned genes were analyzed using quantitative Real time- PCR. Results and dissection The highest expression of the AsFLC in all three clones were observed at 4 weeks after culture (before vernalization). Its expression in non-bolting clone at 4 weeks after planting was 2.03 and 1.13 times more than bolting and semi-bolting garlic, respectively. After vernalization AsFLC expression decreased in the meristem of the all three clones. The decrease in the relative expression of AsFLC in bolting garlic occurred at a faster rate compared to the other two garlic clones. Then the relative expression of the AsSOC1 was increased in the meristem during vegetative to reproductive transition phase (12 weeks after planting). The highest AsSOC1 expression was observed in the meristem of bolting garlic at 12 weeks after planting which was 10.98 and 18.41 times more than the meristem of semi-bolting and non-bolting garlic, respectively. AsAP1 was expressed in the meristem of three clones in the vegetative to reproductive phase, but its highest expression was in bolting clone at 12 weeks after planting and was 1.22 and 3.64 times more than the meristem of semi-bolting and non-bolting clone respectively. AsAP2 was just expressed in the meristem of semi-bolting and bolting clones and after reproductive transition. The highest expression of the AsAP2 was observed at 16 weeks after planting in the meristem of semi-bolting and bolting garlic, which was higher in bolting garlic (2.33 times) in comparison to semi–bolting garlic. Decreases in the expression of the AsFLC during vernalization and increases in the expression of the AsSOC1 and AsAP1 during vegetative to reproductive phase in the meristem may lead to scape formation in bolting garlic. But the higher AsFLC and the lower AsSOC1 and AsAP1 expression in the meristems of non- and semi bolting garlics in comparison to bolting garlic inhibit scape formation. So that in non-bolting garlic scape aborts and in semi-bolting garlic short and thin scape formed in the middle of leaves. According to the results AsSOC1 and AsAP1 were expressed in the inflorescence of semi-bolting and bolting garlic. AsSOC1 and AsAP1 relative expression in the inflorescence of bolting garlic were 4.28 and 11.25 times more than semi-bolting garlic, respectively. AsAP2 was just expressed in the inflorescence of the bolting clone but wasn’t expressed in the inflorescence of semi-bolting garlic. The differences in the expression pattern of AsSOC1, AsAP1 and AsAP2 in the inflorescence of the garlic clones could be the reason of the abnormal inflorescence in semi-bolting garlic.Conclusion:Finally, it seems that AsFLC is as a flowering inhibitor and AsSOC1 and AsAP1 are as flowering integrators in bolting garlic. As AsFLC expression decreased after vernalization and AsSOC1 and AsAP2 were expressed highly in the time of vegetative to reproductive transition in the meristem of all clones, and there was difference in the bolting of clones, it is suggested that these genes may influence flower induction but their low relative expression cause incomplete bolting in semi-bolting garlic and forbid bolting in non-bolting garlic.
Research Article
Postharvest physiology
mina bahrami; Mehdi Jahani; mohmmad Hossein Aminifard
Abstract
IntroductionLong term storage of pomegranate fruit is compromised bypathogens that cause postharvest rots and decay. Pomegranatesare predisposed to attack from various pathogens at pre- and/orpostharvest stage, which has a significant impact on fruit qual-ity and storage life. These pathogens often cause ...
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IntroductionLong term storage of pomegranate fruit is compromised bypathogens that cause postharvest rots and decay. Pomegranatesare predisposed to attack from various pathogens at pre- and/orpostharvest stage, which has a significant impact on fruit qual-ity and storage life. These pathogens often cause damage to thetissues, thereby making the fruit unsaleable. A significant portionof pomegranate pre- and postharvest losses is attributed to dis-eases associated with various bacterial and fungal species. The use of plant essential oils is considered as a new idea in reducing post-harvest waste and increasing the storage life of horticultural products and controlling fungal contamination in developed agriculture. This study was conducted with the aim of determining the antifungal effect of some herbal essential oils in different concentrations on pomegranate fruit to prevent the growth of Penicillium fungus in vitro and in vivo.Materials and Methods In order to investigate the effect of the essential oils of some medicinal plants on the fungus Penicillium sp. in pomegranate fruit in In vivo and In vitro conditions, a factorial experiment was conducted in the form of a completely randomized design with three replications at the Faculty of Agriculture of Birjand University in 2022. The first factor included the type of essential oil: Ginger, Zenian, Tarragon and Cardamom essential oils, and the second factor included the concentration of essential oil at five levels (0, 200, 400, 600, and 800 microliters/liter). The index measured in in vitro conditions was the radial growth of the fungus. The indicators measured in vivo conditions include appearance of the fruit, TSS, pH, total acidity (meq/g), total phenol (mg galic acid/100 f FW), antioxidant (%), antocyanin (mg/g) and tissue firmness. Finally, the experimental data were statistically analyzed using SAS program and comparison of means was performed by LSD test at 5% probability level.Results and Discussion The results showed that the increase in the concentration of essential oils also increases their antifungal activity, so that the highest inhibition rate was observed in Ginger essential oil and the lowest in Cardamom essential oil on the growth of the fungus colony. The most inhibitory effect of fungi included the essential oil of Ginger, Zenian, Tarragon and Cardamom, respectively. Also, lack of growth in essential oil of Ginger was observed in all samplings. The above results showed that the essential oils were able to stop the growth of the fungus colony and show a favorable inhibitory effect. In In vivo conditions, the best fruit appearance was obtained in pomegranates impregnated with Zenian and Ginger essential oils, and the least was observed in Cardamom essential oil. Ginger essential oil had the highest amount of soluble solids. The highest pH value of fruit juice was obtained from Ginger essential oil and the lowest pH value was obtained from Tarragon and Cardamom essential oils. The fruits treated with Tarragon essential oil had the highest total acidity. The fruits treated with Ginger essential oil had the highest amount of total phenol. The statistical comparison of the averages showed that the highest percentage of antioxidants was observed in the Ginger essential oil treatment and the lowest antioxidant percentage was found in the Tarragon essential oil treatment. The results showed that the infected fruits treated with Zenian essential oil with a concentration of 800 microliters/liter had the highest antioxidant. The results of the comparison of the averages related to the effect of the type of essential oil show that Cardamom and Ginger essential oils had the highest and lowest anthocyanin content, respectively. In this experiment, Ginger essential oil had the highest hardness of the fruit texture and the lowest index was obtained from the essential oil of Zenian.ConclusionsIn general, the results of this research showed that essential oils have antifungal properties in the control of fungal disease after harvest; In-vitro tests showed that Cardamom essential oil had a very low fungicidal effect and in the control of Penicillium sp. Ginger essential oil had the most fungicidal effect. Antifungal properties increased with increasing the concentration of essential oil, but the antifungal property of Ginger essential oil was 100% in 400-800 concentrations used. In natural conditions, Ginger and Zenian essential oil has good antifungal activity against Penicillium sp. Considering the biodegradability and low toxicity of plant essential oils, the use of Ginger and Zenian essential oil is recommended to increase the shelf life of food products and can be used as disease controllers in agriculture.
Research Article
Medicinal Plants
Mahdi moradi; Hossein Nastari Nasr-abadi; Mohammadnaser Modoodi; vahid shamsabadi
Abstract
Introduction Moldavian balm (Dracocephalum moldavica L.) is an annual herbaceous plant that belongs to the Lamiaceae family. It is known for its production of essential oils and its medicinal-aromatic properties. Moldavian balm’s essential oil is used for food, cosmetics, flavorings, and pharmaceutical ...
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Introduction Moldavian balm (Dracocephalum moldavica L.) is an annual herbaceous plant that belongs to the Lamiaceae family. It is known for its production of essential oils and its medicinal-aromatic properties. Moldavian balm’s essential oil is used for food, cosmetics, flavorings, and pharmaceutical purposes. Abiotic stresses include drought, soil salinity, flooding, extremes of temperature, and contamination with organic pollutants and heavy metals hamper plant growth and productivity. In recent decades, heavy metal (HM) pollution has spread across natural and anthropic ecosystems posing inevitable, serious health risks. Soil microbiota plays an important role in the sustainable production of the different types of agrosystems. Mycorrhizae (a combination of mycelium of the fungus and the roots of the plant) form networks that capture water and nutrients from the soil, which facilitate the acquisition of the plant. Arbuscular Mycorrhizal (AM) Fungi play a crucial role in mitigating the oxidative damage caused by heavy metal stress in different plant species. Thus, interaction between mycorrhizae, and plants can be an excellent strategy for sustainable agricultural production The aim of this study was to determine the effects of mycorrhizal arbuscular on improving heavy metal tolerance in moldavian balm, a medicinal and aromatic plant.Material and MethodsAn experiment was conducted to study the effect of mycorrhizal arbuscular fungi on growth characteristicsas, quantitative and qualitative yield of Moldavian Balm, under heavy metals stress at Research Greenhouses of College of Agriculture, University of Torbat-e Jam. Treatment included mycorrhiza (inoculated and non-inoculated) and heavy metals including lead (0, 150 and 300 mg/kg) and cadmium (0, 40 and 80 mg/kg) Which arranged in factorial based on completely randomized design whit 4 replications. Growth criteria, yield components, essential oil content and photosynthetic pigments were evaluated. In this study, different morphological traits (plant height, root length, root volume, stem diameter, Leaf length, Leaf width, Leaf area, stem diameter, Number of branches), vegetative parameters (Biomass fresh and dry weight, root fresh and dry weight), photosynthetic pigment concentrations and essential oil content were measured analyzed. Data analysis of variance (ANOVA) was performed using IBM SAS software (Version 9.1) and the differences between means were assessed using Duncan’s multiple range tests at p≤ 0.05.Results and DiscussionResults showed that with increasing the concentration of heavy metals in the soil especially that of Cd, most of the growth characteristics and yield components of these plants were significantly reduced as compared to those of controls. Mycorrhizal inoculation improved these traits where plants were grown under heavy metals stress. The highest biomass fresh and dry weight was observed in mycorrhizal plants grown in non-contaminated medium, which was significantly higher than those of other treatments. The highest biomass fresh weight was measured in non-stressed mycorrhizal plants. Biomass fresh weight in non-mycorrhizal plants of Pb150 did not differ significantly from that of mycorrhizal plants of Pb150. biomass fresh weight in mycorrhizal and nonmycorrhizal plants stressed by Cd40 or Cd80 was lower compared to mycorrhizal and non-mycorrhizal plants grown in non-contaminated media or contaminated media with Pb150 or Pb300. Furthermore, the lowest biomass fresh weight was observed in non-mycorrhizal plants stressed by Cd80. Root fresh and dry weight of mycorrhizal plants was significantly higher than that of non-mycorrhizal plants. With increasing Cd or Pb concentration in soil, Root fresh and dry weight was decreased as compared to that of controls. Inoculation with mycorrhizal improved the photosynthetic pigment concentrations under heavy metals stress. The highest percentage of essential oil content (1.3% v/w) was observed in mycorrhizal plants stressed by Pb150, while the lowest percentage (0.53% v/w) was evident in non-mycorrhizal plants polluted with Cd80, With increasing concentration of the heavy metals, essential oil of moldavian balm was significantly decreased, but the essential oil content in mycorrhizal plants was significantly higher than that measured in non-mycorrhizals. ConclusionArbuscular Mycorrhizal (AM) Fungi are widely believed to support plant establishment in soils contaminated with heavy metals, because of their potential to strengthen defense system of the AM mediated plants to promote growth and development. Overall, mycorrhizal inoculation of moldavian balm has promoted plant growth and, in addition, mycorrhization has enhanced yield as well as active substances in moldavian balm grown in the heavy metals stress condition. However, these approaches show promise in mitigating the adverse effects of heavy metals stress and improving the overall health and productivity of plants. Based on the enhanced physiological and biochemical responses, as well as increased essential oil content, it is recommended to use arbuscular mycorrhizal fungi fertilization under heavy metals.
Research Article
Postharvest physiology
Leila Taghipour; Mehdi Hosseinifarahi; Parisa Hayati; Pedram Assar
Abstract
Extended Abstract IntroductionThe physalis (Physalis peruviana L.) is one of the most important members of the Solanaceae family. It is cultivated as a perennial in tropical regions and as an annual in temperate regions. The fruit is a round berry that can be consumed fresh, dried, or used in desserts ...
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Extended Abstract IntroductionThe physalis (Physalis peruviana L.) is one of the most important members of the Solanaceae family. It is cultivated as a perennial in tropical regions and as an annual in temperate regions. The fruit is a round berry that can be consumed fresh, dried, or used in desserts and jams. Physalis fruits are rich in minerals, vitamins, and phytochemicals with anti-tumor and anti-inflammatory properties, making them a popular "superfood." Globally, there is increasing demand for this crop as a healthy and beneficial food, including in Iran, although detailed statistics on its cultivation in Iran are lacking. Physalis is a climacteric fruit with a very short postharvest life, typically lasting a maximum of 5 days. Therefore, there is a need for safe postharvest treatments to maintain high quality and extend its shelf life.Materials and MethodsFully orange-colored physalis fruits with completely yellow calyxes were harvested from a commercial greenhouse in Pasargad, Fars province. The fruits were quickly transported to the lab, visually evaluated, washed with deionized water, and air-dried. Melatonin solutions at concentrations of 100, 200, and 300 μM were prepared. Sixty fruits were dipped in each melatonin solution for 5 minutes, with distilled water used as the control. The treated fruits were then air-dried for 30 minutes, packaged in polyethylene bags with 3% perforation, and stored at 10 °C with 90 ± 5% relative humidity for 21 days. Evaluations were conducted weekly.Results and DiscussionOverall, postharvest treatment with melatonin led to a reduction in respiration rate and polyphenol oxidase (PPO) activity in the juice, as well as an improvement or maintenance of skin carotenoid content, total soluble solids (TSS), titratable acidity (TA), ascorbic acid, total phenols, phenylalanine ammonia-lyase (PAL) enzyme activity, and total antioxidant activity in the juice. After 21 days of storage and at the end of the experiment, the assessment of all these attributes revealed that fruits treated with 300 μM melatonin were superior in terms of nutritional value, appearance, and postharvest oxidative stress response mechanisms compared to the other experimental groups. There was no significant difference in total soluble solids and titratable acidity among the fruits treated with different concentrations of melatonin; however, fruits treated with the two higher concentrations of melatonin showed the lowest respiration rate and the highest ascorbic acid content in the juice. Furthermore, fruits treated with 300 μM melatonin exhibited higher levels of total phenols, PAL enzyme activity, total antioxidant activity, and skin carotenoids compared to all other experimental groups, while also showing the lowest PPO enzyme activity.ConclusionTreating physalis fruits with exogenous melatonin, especially at concentration of 300 μM, can significantly enhance their postharvest quality and storability by modulating various physiological and biochemical processes. This approach has the potential to improve the marketability and economic value of harvested physalis as a high-value horticultural crop.Extended Abstract IntroductionThe physalis (Physalis peruviana L.) is one of the most important members of the Solanaceae family. It is cultivated as a perennial in tropical regions and as an annual in temperate regions. The fruit is a round berry that can be consumed fresh, dried, or used in desserts and jams. Physalis fruits are rich in minerals, vitamins, and phytochemicals with anti-tumor and anti-inflammatory properties, making them a popular "superfood." Globally, there is increasing demand for this crop as a healthy and beneficial food, including in Iran, although detailed statistics on its cultivation in Iran are lacking. Physalis is a climacteric fruit with a very short postharvest life, typically lasting a maximum of 5 days. Therefore, there is a need for safe postharvest treatments to maintain high quality and extend its shelf life.Materials and MethodsFully orange-colored physalis fruits with completely yellow calyxes were harvested from a commercial greenhouse in Pasargad, Fars province. The fruits were quickly transported to the lab, visually evaluated, washed with deionized water, and air-dried. Melatonin solutions at concentrations of 100, 200, and 300 μM were prepared. Sixty fruits were dipped in each melatonin solution for 5 minutes, with distilled water used as the control. The treated fruits were then air-dried for 30 minutes, packaged in polyethylene bags with 3% perforation, and stored at 10 °C with 90 ± 5% relative humidity for 21 days. Evaluations were conducted weekly.Results and DiscussionOverall, postharvest treatment with melatonin led to a reduction in respiration rate and polyphenol oxidase (PPO) activity in the juice, as well as an improvement or maintenance of skin carotenoid content, total soluble solids (TSS), titratable acidity (TA), ascorbic acid, total phenols, phenylalanine ammonia-lyase (PAL) enzyme activity, and total antioxidant activity in the juice. After 21 days of storage and at the end of the experiment, the assessment of all these attributes revealed that fruits treated with 300 μM melatonin were superior in terms of nutritional value, appearance, and postharvest oxidative stress response mechanisms compared to the other experimental groups. There was no significant difference in total soluble solids and titratable acidity among the fruits treated with different concentrations of melatonin; however, fruits treated with the two higher concentrations of melatonin showed the lowest respiration rate and the highest ascorbic acid content in the juice. Furthermore, fruits treated with 300 μM melatonin exhibited higher levels of total phenols, PAL enzyme activity, total antioxidant activity, and skin carotenoids compared to all other experimental groups, while also showing the lowest PPO enzyme activity.ConclusionTreating physalis fruits with exogenous melatonin, especially at concentration of 300 μM, can significantly enhance their postharvest quality and storability by modulating various physiological and biochemical processes. This approach has the potential to improve the marketability and economic value of harvested physalis as a high-value horticultural crop.
Research Article
Ornamental plants
Maryam Ayaseh; Mohsen Kafi; Mahdi Khansefid; Majeed Shokrpour; Rohangiz Naderi
Abstract
Title
Physico-chemical response and seedling growth of three aromatic plants, Tanacetum parthenium, Achillea millefolium, and Zygophyllum eurypterum to salinity stress
2. Introduction
Today, a significant factor limiting plant growth is the rising salinity of soil and water, which poses ...
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Title
Physico-chemical response and seedling growth of three aromatic plants, Tanacetum parthenium, Achillea millefolium, and Zygophyllum eurypterum to salinity stress
2. Introduction
Today, a significant factor limiting plant growth is the rising salinity of soil and water, which poses challenges to food security, ecological stability, and agricultural systems. Numerous studies indicate that salinity hampers seed germination and early plant development by inducing osmotic stress, ion toxicity, and oxidative stress. Utilizing salinity-tolerant aromatic plants in saline areas can effectively enhance landscapes. Therefore, cultivating valuable plant species that meet high ecological demands may be the best strategy to mitigate salinity stress in agriculture and natural resources. These species can serve as alternative crops for oil seed production, food products, fodder, and both medicinal and ornamental uses. Tanacetum parthenium, Achillea millefolium, and Zygophyllum eurypterum are examples of medicinal and aromatic herbaceous perennials with low input requirements. They can be grown for various purposes, including ornamental use in dry and saline climates, as well as in the food, perfumery, cosmetics, and medicinal industries. Successful cultivation of these valuable plants in stressful ecosystems necessitates investigating their early growth responses to salinity stress. This research aims to identify suitable conditions for the successful establishment of these plants, ultimately leading to increased production.
3. Materials and Methods
In order to investigate the physicochemical responses and the early growth of seedlings in the shoot and root of three plants, T. parthenium, A. millefolium and Z. eurypterum to different levels of zero salinity stress (control), 1.5, 3 and 6 dS/m, an experiment was conducted as factorial based on completely random design with three replications using a completely randomized design in a greenhouse under pot conditions in Department of Horticultural Science and Green Space Engineering at the University of Tehran. Seeds from three plant species—T. parthenium, A. millefolium, and Z. eurypterum—were obtained from the Research Institute of Forests and Rangelands (RIFR) in Karaj province, Iran. Prior to planting, the seeds were sterilized for one minute in a 2% sodium hypochlorite solution and then washed three times with sanitized water. The seeds were then planted in plastic pots (45, 66, and 23 cm) filled with a mixture of clay, sand, and perlite, at a depth of three centimeters, under greenhouse conditions. Sodium chloride was used to create salinity stress, exposing all studied plants to four salinity levels for a duration of six weeks. The greenhouse maintained a temperature of 20°C at night and 25°C during the day, with a relative humidity of 60-80%. All physiological and biochemical measurements were conducted after six weeks, once the plants were fully established. It is important to note that all samples were washed with distilled water post-sampling, the surface water was removed using filter paper, and samples were immediately stored at -80°C until measurements were taken
4. Results and Discussion
The results of this study indicated that the concentration of photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll) decreased with increasing salinity levels, which correlated with a reduction in the growth length of both shoots and roots. The results indicated that under severe salinity stress, oxidative damage increased, leading to higher levels of fat peroxidation (MDA) and reduced cell membrane stability. This decrease in stability was linked to a reduction in photosynthetic pigments, ultimately disrupting optimal plant early growth. At all salinity levels, the total antioxidant capacity of A. millefolium leaves was greater than that of its roots. Among all three plants and salinity levels, the highest total antioxidant capacity of 78.57% was observed in the roots of Z. eurypterum under a salinity condition of 3 dS/m. Conversely, the lowest total antioxidant capacity, at 25.38%, was found in the leaves of T. parthenium under non-saline conditions. The results indicated that the potassium to sodium ratio at equilibrium varied significantly depending on the plant species, plant organ, and salinity concentration and the highest ratio was found in the aerial parts of T. parthenium.
5. Conclusion
Overall, based on the biochemical and physiological responses to salinity stress, all three plants exhibited a relatively high tolerance. However, the T. parthenium plant demonstrated the greatest tolerance to salinity stress among them. Therefore, the cultivation of these three species, which possess ornamental, aromatic, and medicinal value, can serve as a viable solution for the ecological sustainability of saline ecosystems and their integration into urban landscaping.
6. Acknowledgement
The authors are grateful for the scientific assistance of Dr. Behrouz Malekpour
7. Keywords: Antioxidant Capacity, Aromatic Plants, Ion homeostasis, Salinity Stress
Research Article
Pomology
Seyed Asghar mousavi; Maryam Tatari
Abstract
Evaluation of Drought Tolerance in Some Almond Commercial Cultivars (Prunus dulcis Mill.) on GN Vegetative Rootstock
Introduction
Peach×almond (GN) hybrid rootstocks have favorable characteristics such as ease of rooting, favorable growth vigour, resistance to nematodes, calcareous and dry soil (Babadaei ...
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Evaluation of Drought Tolerance in Some Almond Commercial Cultivars (Prunus dulcis Mill.) on GN Vegetative Rootstock
Introduction
Peach×almond (GN) hybrid rootstocks have favorable characteristics such as ease of rooting, favorable growth vigour, resistance to nematodes, calcareous and dry soil (Babadaei et al., 2018). Currently, water deficiency because of the decrease in rainfall is one of the important concerns and a key limitation in the production of agricultural products (Hass et al, 2021). Under drought stress, plant cell membranes are sensitive to electrolyte leakage. Membrane leakage is caused by uncontrolled free radicals and leads to lipid peroxidation (Cheng et al. 2018). Since tolerance to drought stress is the result of the interaction of morphological and physiological traits of plant, therefore, a combination of different traits that have a direct relationship with drought tolerance can be used as selection criteria to screen the ideal cultivar (Karimi et al., 2015).
Material and methods
This research carried out during the years 2020 and 2021 at the Chahartakhteh station affiliated to the Center for Research and Education of Agriculture and Natural Resources in Chaharmahal and Bakhtiari Province. The almond seedlings included Shahroud 6, 7, 8, 10, 12, 13 and 21, that all of them grafted on the GN rootstock, along with GN rootstock, subjected to different drought stress treatments in June for four months. Drought stress treatments included 70% of field capacity (control or no drought stress), 50% field capacity (mild stress), 30% field capacity (moderate stress) and 10% field capacity (severe stress). A counter determined the amount of irrigation in each treatment, and a Time-Domain Reflectometry (TDR) used to measure the soil moisture. Before the experiment, the physicochemical properties of the soil measured. The measured morphological traits included leaf area, percentage of leaf abscission, and shoot fresh and dry weight. These traits measured four months after subjecting to water stress. The evaluated physiological traits included electrolyte leakage (EL), relative leaf water content (RWC), leaf chlorophyll, proline and malondialdehyde (MDA). These traits also measured four months after subjecting to water stress. The experiment conducted in split plots based on a randomized complete block design in three replications and four seedlings in each experimental unit. The main plot included water drought treatments and the sub plot included almond cultivars. Data analysis carried out using SAS software version 9.2 and comparison of mean data conducted based on LSD test at a five percent probability level.
Results and discussion
As the intensity of drought stress increased, the fresh and dry weight of shoot decreased in almond cultivars and GN rootstock. At soil humidity of 10% field capacity, Shahroud 8 showed higher amounts of shoot fresh weight (785 g). The lowest fresh and dry weight of the shoot and the highest leaf abscission were observed under severe drought stress (10% of the field capacity) in Shahroud 13. The increase in drought levels led to a decrease in the leaf area in the studied cultivars and rootstock of almond. Shahroud 8 had more chlorophyll content than other cultivars at the most severe stress level, and Shahroud 13 showed the lowest chlorophyll b content at 10% humidity of field capacity. A significant decrease in leaf chlorophyll concentration under drought stress has also been reported in previous research (Schlemmer et al., 2005; Gohari et al., 2023). A further decrease in chlorophyll could be due to a drastic decrease in RWC under severe stress conditions. According to Ranjbar et al. (2022), the amount of RWC decreased by 32 to 44% under stress conditions in the K13-40 grafted cultivar on the rootstock of bitter almond No. 32. In the current research, the biggest decrease in RWC was in Shahroud 13 with a decrease of 36.85%. The lowest decrease in RWC was also observed in the GN rootstock with a decrease of 20.94%. Shahroud 13 and GN rootstock showed the highest and lowest electrolyte leakage at the highest stress level, respectively. Karimi et al. (2023) also found a significant increase in EL in White, Mamai and Ferragnes cultivars due to the higher sensitivity of these cultivars to water loss. The most difference in MDA value was observed in Shahroud 13 with an increase of 186.35% and the lowest difference was found in Shahroud 10 with an increase of 84.58%. Shahroud 6 produced the highest content of proline under severe water stress. According to the results, Shahroud 13 and then Shahroud 6 were recognized as the most sensitive cultivars. Shahroud 8 and 12 were among the tolerant cultivars. Other cultivars were also between these two groups.
Conclusion
Shahroud 13 was recognized as the most sensitive cultivar with the lowest fresh and dry weight, RWC and the highest ion leakage and MDA at the most severe stress level. After that, Shahroud 6 had high sensitivity. Shahroud 8 was recognized as the most drought tolerant cultivar due to its lowest MDA content, the highest chlorophyll a and b and RWC in the most level of drought. After Shahroud 8, Shahroud 12 was including the tolerant cultivars. Tolerant cultivars can be used in future studies to evaluate the possibility of planting these cultivars in areas with water shortage problems
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