اثر هیدروپرایمینگ و اسموپرایمینگ با GA3 و KNO3 بر جوانه‌زنی بذر ناترک (Dodonaea viscosa) تحت شرایط شوری

نوع مقاله : مقالات پژوهشی

نویسندگان

دانشگاه شهید چمران اهواز

چکیده

ناترک (Dodonaea viscosa) درختچه‌ای زینتی، همیشه سبز دایمی و دارای گسترش وسیعی در سرتاسر مناطق گرم دنیا می‌باشد. به منظور بهبود جوانه‌زنی بذر ناترک، اثرات آماده‌سازی (پرایمینگ) و سطوح مختلف شوری بر شاخص‌های جوانه‌زنی بذر این گیاه مورد مطالعه قرار گرفت. در این بررسی دو آزمایش متوالی بصورت طرح کاملاً تصادفی با چهار تکرار انجام شد. در آزمایش اول تیمارها شامل هیدروپرایمینگ (تیمار با آب مقطر به عنوان شاهد) و اسموپرایمینگ شامل (نیترات پتاسیم 5/0 و 1 درصد و اسید جیبرلیک 50 و 100 میلی‌گرم در لیتر) و در آزمایش دوم بر اساس بهترین نتایج آزمایش اول (تیمار اسید جیبرلیک 50 میلی گرم در لیتر) تیمار شوری با کلرید سدیم (0، 3، 6، 9 و 12 دسی‌زیمنس بر متر) مورد مطالعه قرار گرفت. نتایج آزمایش اول نشان داد تیمار اسموپرایمینگ اثر معنی‌داری روی درصد جوانه‌زنی، طول ریشه‌چه و ساقه‌چه و وزن تر ریشه‌چه داشت. اسید جیبرلیک50 میلی‌گرم در لیتر نسبت به بقیه تیمارها موثرتر بود. حداکثر درصد جوانه زنی (68 درصد)، طول ریشه‌چه (25/41 میلی‌متر) و ساقه‌چه (50/71 میلیمتر) و وزن تر ریشه‌چه (12/0 گرم) تحت تاثیر جیبرلیک اسید 50 میلی‌گرم در لیتر بدست آمد. نیترات پتاسیم 1 درصد کمترین تاثیر را بر درصد جوانه‌زنی (50 درصد)، طول ریشه‌چه (75/21 میلی‌متر) و وزن تر ریشه‌چه (06/0) گرم داشت. در آزمایش دوم اسید جیبرلیک 50 میلی‌گرم در لیتر در سطح احتمال 1 درصد بر تمام شاخص‌های جوانه‌زنی اثر معنی‌دار مثبت داشت و در سطح شوری 3 و 12 دسی‌زیمنس بر متر به ترتیب بیشترین و کمترین تاثیر را داشت. بطور کلی اسید جیبرلیک 50 میلی‌گرم در لیتر در سطح شوری 3 دسی‌زیمنس بر متر برای تکثیر ناترک با بذر نتایج بهتری را نشان داد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Hydro and Osmo-Priming in Combination with GA3 and KNO3 on Seed Germination of Dodonaeaviscosaunder Salinity Conditions

نویسندگان [English]

  • S. Pour Mombeini
  • noorolah moalemi
Shahid Chamran University, Ahwaz
چکیده [English]

Introduction: Dodonaeaviscosa is an evergreen and perennial shrub which distributed widely in warm regions throughout the world. Untreated seeds germinatewell, but to shorten germination, seeds werefloated in hot water for 30 seconds, then cooled down rapidly. In addition, scarification as pre-treatment and dipping the seed in boiling water improve seed germination. Under salinity,the level of abscisic acid increases more than gibberellic acid which prevents seeds germination. Accumulation of Na+ and Cl- ions can lead to changes in water status in plant tissues and creating a series of second stress, such as oxidative stress. In priming, seeds are authorized to absorb some water, sothat, the early stages of germination are donewithout root emerging. The country of Iran and especiallyKhozestan province is located in arid and semi-arid region of the world, sounder such conditionand dueto high temperature and low rainfall, and salinity of water sources and media, D.viscosasexual reproduction is suffered by serious challenges. Consequently,the objective of this study was to investigate the effectsof hydro and osmo-priming in combining withgibberellic acid and potassium nitrate on seed germination of D.viscosaunder salinity stress.
Materials and Methods: Two experiments in 2010 wereconducted separately and consecutively in completely randomized design with five treatments and four replications in the plant propagation laboratory of faculty of Agriculture, ShahidChamran University of Ahvaz (latitude 31.33° N, longitude 48.69° E, altitude 23 m). In the first experiment, 500 seeds were selected from seed lot, and were disinfectedwith sodium hypochlorite 2.5%, afterwards, divided into five categories (each category consisted of 100 seeds in four replicates) and were treated as : 1) Osmo-priming with gibberellic acid 50 and 100 mg.l-1 and potassium nitrate 0.5 and 1 % for 12 h. 2) hydro-primed with distilled water (as control) for 12 h. A week later second experiment wascarried out (with 500 seeds from some initial seedlot) base of the best result obtained in the first experiment to study theeffects of salinityonseed germination. Seeds weretreated withgibberellic acid(50 mg.l-1)for 12 h. During germination, seeds were irrigated with different levels of salinity, including 0.0 (distilled water as control), 3, 6, 9 and 12 ds.m-1using NaCl salt (as halopriming). Analysis of data (ANOVA) wasperformed by procedure within thestatistical software MSTAT-C and means were separated by multiple-rangetest of Duncan’s at (p

کلیدواژه‌ها [English]

  • Germination
  • Osmotic stress
  • Priming
  • Plumuleand radicle
1- Agrawal R. L. 2004. Seed Technology. Oxford and IBH Pub. Co. LTD., New Dehli.
2- Ajmal khan B.G., and Weber D. 2004. Action of plant growth the regulators and salinity on seed germination of ceratoides lanata. Canadian Jurnale of Botany, 82(1) :37-42.
3. Anonymos. 1992. A selection of useful trees and shrobs for kenya: notes on their identification, propagation and management for use by agricultural and pastoral Communities. International Centre for Research in Agroforestry: Nairobi, Kenya, PP: 225.
4- AOSA (Association of Official Seed Analysis). 1991. Rules for testing seeds. Journal of Seed Technology,
12: 18-19.
5- Arin L.E., and Kirak D.Y. 2003. The effect of pre-sowing treatments on emergnce and seedling growth of tomato seed (Liycopersicon esculentum Mill.) under several stress conditions. Pakistan Journal of Biological Science, 6(11):990-994.
6- Artola A., Carrillo-Castaneda G., and Santos. G.D.L. 2003. Hydro priming: a strategy to increase Lotus
corniculatus L. seed vigor. Seed Science and Technology, 31: 455-463.
7- Atia A. 2006. Alleriation of salt induced seed dormancy in the Perennial halophyte Crithmum maritimuml, (Apiaceae). Pakistan Jurnale of Botany, 3815: 1367-1372
8- Baskin J.M., and Baskin C.C. 2000. Taxonomy, Anatomy and evolution of physical dormancy in seeds. Plant Species Biology, 15: 139-1520
9- Basra S.M.A., Ashraf M., Iqbal N., khaliq A., and Ahmad R. 2004. Physiological and biochemical as pects of pre-sowing heat stress of cottonseed. Seed Science and Technology, 32: 765-774
10- Boqumila B., Bert Van D., and Mieczuslaw G. 2005. Effect of water supply methods and seed moisture content on germination of China aster (Callistemon chinensis) and Tomato (Lycopersicom esculenton Mill). Seed Research Institue of Phonology and Floriculture, UL. Pomologicza, 18: 96-100.
11- Burnett S., Thomas P., and Van Iersel M. 2005. Post germination drenches with PEG-8000 reduce growth of salvia and marigolds. Horticulture Science, 40(3): 675-679.
12- Camberato J., and Mccarty B. 1999. Irrigation water quality :part I. salinity. South Carolina Turfgrass Foundation New, 6(2) :6-8.
13- Catalan I., Balzaring Z., Talesnik E., Sereno R., and karlin U. 1994. Effect of salinity on germination and seedling growth of prosopis flexuous (D.C.). Fores Ecology and Management, 63: 347-357
14- Chojonowski F.C., and Come D. 1997. Physiological and biochemical changes indoced in sunflower seed by osmopriming and subsequcnt drying, storage and aging. Seed Science Research, 7: 323-331.
15- Demir kaya M., Okcu G., Atak M., Cikili Y., and kolsaricl O. 2006. Seed treatment to overcome sait And drought stress during germination in sunflower (Helianthus annuus l.). European Journal of Agronomy, 24: 291-295.
16- Farooq M., Basra S.M., and Ahmad A.N. 2007. Improving the performance of transplanted rice
by seed priming. Plant Growth Regulation, 51(2): 129-137.
17- Francois- leland E. 1985. Salt Injury to ornamental shrubs and ground covers. Science and Education Administration Home and Garden Bulletin 231, USDA. PP.10
18- Gao Y.P., Young L., Bonham-Smith P., and Gusta L.V. 1999. Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions. Plant Molecular Biology, 40: 635-444
19- Ghassemi G., and Esmaeilpour B. 2008. The Effect of Salt Priming on the Performance of Differentially Matured Cucumber (Cucumis sativus) Seeds. Notulae Botanicae Horti Agrobotanici, Cluj-Napoca, 36 (2): 67-70.
20- Hamzai J., Shayanfar R. and Fotoohi K.1391. The effect of seed priming on some qualitative and quantitative characteristics two sugar beet (Beta vulgaris L.). Journal of Production and processing of agricultural and horticultural crops. Journal of production and processing of agricultural and horticultural productsm, 2(6).
21- Hanson B., Grattan S.R. and Fulton A. 1999. Agricultural salinity and Drainage, University of California, Davis, PP: 160.
22- Hardegree S.P., and Van Vactor S.S. 2000. Germination and emergence of primed grass seeds under field and simulated field temperature regimes. Annals of Botany, 85:379-390.
23- Hus J.l., and Sung J.M. 1997. Antioxidant role of glutatione associated with accelerated agina and hydration of triploid watermelon seeds. Physiologa plantarum, 100: 967-974.
24- ISTA (International Seed Testing Association). 2008. International rules for seed testing. Seed Science and Technology, 24:155- 202.
25- Jamil M., and Rha E.S. 2007. Gibberllic acid (GA3) enhance seed water uptake, germination and early seedling growth in sugar beet under salt stress. Pakistan Journal of Biological Science, 10: 654-658.
26- Khaleghi E., and Ramin A. 2005. The effect of salinity on growth and development indicators grass (cynodon dactylon, Lolium perenne and Festuca arundinacea). Journal of Agricultural Sciences and Natural Resources, 9 (3). (In persian with english abstract).
27- Lafond G.P., and Baker R .J. 1986. Effects of temperature, moisture stress, and seed size on germination of nine spring wheat culturar. Crop Science, 26: 563-567.
28- Lee S.S., kim J.H., Hong S.B., Yun S.H., and Park E.H. 1999. Priming effect of rice seed on seedling establishment under adverse soil conditions. korean Journal of Crop Science, 44: 194-198.
29- Lemaire S.F., Maupas P., Cournede H., and. Reffye P. 2008. A morphogenetic crop model for sugar-beet (Beta vulgaris L.). International Symposium on Crop Modeling and Decision Support: ISCMDS 2008, Nanjing, China, pp. 19-22.
30- Little E.L., and Skolmen R.G. 1989. Common Forest trees of Hawaii (Native and Introduced ). Agriculture hand book, 679.U.S. Department of Agriculture, Washington, District of Columbia, P: 32L.
31- Mc-Donald M.B. 1999. Seed deterioration: physiology, repair and assessment., Seed Science and Technology, 27: 177 -237.
32- Mauromicale G., and Cavallaro V. 2000. Effects of seed osmopriming on the harvest time and yield of Cucurbita pepo L. Acta Horticulture (ISHS) 533: 83-88.
33- Murungu F.S., Nyamugafata P., Chiduza C., Clark L J. and Whalley W.R. 2003. Effects of seed priming, aggregate size and soil water potential on emergence of cotton (Gossypium hirsutum L.) and maize (Zea mays L.). Soil and Tillage Research, 74(2): 161-168.
34- Pill W.G., and kilian E. 2000. Germination and emergence of parsley in response to osmotic or matric seed priming and treatment with gibberellins. Horticultural science, 35 (5): 907- 909.
35- Ralph M. 1994. Seed collection of Australian native plants: For revegetation, tree planting and direct seedling (2nd ededition)., Bushland Horticulture, Fitzroy, VIC.
36- Ralph M. 2003. Growing Australian native plants from seed: for revegetation tree planting and direct seedling (2nd edition). Bushland Horticulture, Fitzroy, Victoria.
37- Rani S., Pippalla R., and Mohan K. 2009. Dodonaea Viscosa., Anoverview Journal of Pharmaceutical Research and Health Care (JPRHC), 1:97-112.
38- Ren J., and Taoa L. 2004. Effects of different pre-sowing seed treatments on germination of 10 Calligonum species. Forest Ecology and Management, 195: 291–300.
39- Shaddal M.A. 1990. Response of seeds of lupinus termis and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxin, Plant and Soil, 122: 177-183.
40-Shim S.L., Cheol M.J., Jang Ch.S., Paul R., and Wook K. 2008. Effect of potassium Nitrate priming on seed Germination of seashore paspalum. Horticultural Science, PP: 2259-2262.
41- Sivritepe N.O., Sivritepe H.O., and Eris A. 2003. The effects of Nacl priming on salt to lerance in Melon seedling grown under saline condition. Scientia Horticulture, 97: 229-237.
42- Smok M.A., Chojnowski M., Corbineau F., and Come D. 1993. Effect of osmotic treatment on sunflwer seed germination in relation with temperature and oxygen. In: Come , D. and Corbineau, F. (Eds). Fourth International workshop on seeds basic and aspects of Seed Biology, 3: 1033-1038.
43- Sunchez J.A., Munoz B.C., and Fresneda. 2001. Combine effects of hardening hydration – dehydration and heat shock treatments on the germination of tomato, Pepper and Cucumber. Seed Science and Technology, 29: 691-697.
44- Temesagdie P., and Takano T. 1992. Effect of treated seeds white H2BO3, GA3, KNO3 and ZNSO4 of seedling growth of Sweet Corn and yard long Bean. Natural Science Supplement, 26: 34-40.
45- Tylkowsa K., and Van den balk R.W. 2001. Effects of osmo- and hydropriming on fungal infestation levels and germination of carrot (Daucus carota L.) seeds cantaminated with alternaria spp. Seed Science and Technology, 29: 365-375.
46- Venkatesh S., Reddy Y.S.R., Ramesh M., Swamy M.M., Mahadevan N., and Suresh B. 2008. Pharmacolognostical studies on Dodonaea viscosa leaves. African Journal Of Pharmacy and Pharmacology, 2(4): 83-88.
47- Wahid A., Noreen A., Basra SH.M.A., Gelani S., and Farooq M. 2008. Priming induced metabolic changes in sunflower (Helianthus annuus) achenes improve Germination and seedling growth. Botanical studies, 49: 343-350.