تاثیر حلال‌های مختلف بر میزان ترکیبات فنلی و فعالیت آنتی اکسیدانی میوه عناب (Ziziphus jujube Miller)

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 دانشگاه علوم پزشکی مشهد

چکیده

این تحقیق به منظور بررسی اثر تیمار حلال‌های متانول، اتانول، استون (50، 90 و 100 درصد) و آب مقطر بر روی میزان ترکیبات آنتی اکسیدانی (فنل کل، تانن ها، فلاونوئیدها، آنتوسیانین ها) میوه عناب (Ziziphus jujube Miller)، انجام شد. فعالیت آنتی اکسیدانی هر یک از عصاره‌ها نیز به روش‌های DPPH وFRAP مورد ارزیابی قرار گرفتند. نتایج نشان داد که استون 50 درصد در استخراج ترکیبات فنل و فلاونوئید کل به ترتیب با مقادیر (20/798 میلی گرم گالیک اسید در 100 گرم ماده خشک) و (73/1871 میلی گرم کوئرستین در 100 گرم ماده خشک)، بهترین عملکرد را در میان سایر حلال‌ها داشته است. استخراج آنتوسیانین، با متانول 50 درصد (31/6 میلی گرم سیانیدین 3- گلوکوزید در 100 گرم ماده خشک) و استخراج تانن با متانول 100 درصد (4/669 میلی گرم کاتکین در 100 گرم ماده خشک)، دارای بالاترین بازده استخراج بوده است. در تمامی موارد آب از حداقل قابلیت استخراج متابولیت‌های ثانویه در بین سایر حلال‌ها برخوردار بود. در بررسی خاصیت آنتی اکسیدانی میوه عناب به دو روش DPPH و FRAP مشخص گردید که خاصیت آنتی اکسیدانی آن‌ها مستقیما تحت تاثیر مقادیر ترکیبات فنلی قرار گرفته است. در میان حلال‌های مورد استفاده، استون 50 درصد بهترین بازده را در روش DPPH ( 02/29=IC50) و روش FRAP (11/5 میلی مول آهن II در 100 گرم ماده خشک) نسبت به سایر حلال‌ها داشت. بر اساس نتایج می‌توان گفت درصد مهار کنندگی DPPH و FRAP با کاهش غلظت حلال‌های متانول و استون رابطه مستقیم دارد. نتایج این تحقیق نشان داد که تفاوت در نوع، خلوص و قطبیت حلال‌ها و تفاوت در جزئیات روش استخراج عصاره (مانند زمان استخراج، سرعت اختلاط، نسبت میزان پودر به حلال و اندازه ذرات پودر) بر میزان استخراج متابولیت‌های ثانویه تاثیر می‌گذارند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Different Solvents on Total Phenolic Contents and Antioxidant Activity of Zizyphus jujube Miller Fruits

نویسندگان [English]

  • Gholamhossein Davarynejad 1
  • Seyedeh Faezeh Taghizadeh 1
  • Javad Asili 2
1 Ferdowsi University of Mashhad
2 Mashhad University of Medical Sciences
چکیده [English]

Introduction: Phenolic compounds have an ability to scavenge free radicals and cause the balance of reactive oxygen species (ROS) in our body. This balance prevents atherosclerosis, coronary heart and cancer diseases. Butylated hydroxyl toluene (BHT) is a well-known synthetic antioxidant, which is restricted to be used due to its probable toxic effects. Therefore, replacement of synthetic antioxidants with plant materials with high amounts of antioxidant activity, which protect the body from free radicals and many diseases caused by lipid peroxidation, is an appropriate option. ZiziphusjujubaMiller is one of the forty species belonging to Rhamnaceae family, which produces a great deal of industrial raw materials for horticultural, ornamental, food, and pharmaceutical industries. Antioxidants can be extracted by various solvents and extraction methods. Solvent extraction is the most common method used for separating natural antioxidants. Solvent properties undoubtedly play a key role in the extraction of antioxidative compounds. The type and yield of antioxidant extracted have been found to vary as affected by the solvent properties such as polarity, viscosity and vapor pressure. Therefore, it is difficult to develop a unified standard method for the extraction of antioxidants from all plant materials.
Materials and Methods:
Plant materials Fresh fruits were collected from Birjand, Iran, in late summer 2014. The samples were air dried under the shade at room temperature. Dried fruits were ground by using a mortar and pestle and were separately extracted by distilled water and organic solvents such as methanol, ethanol and acetone (50%, 90% and100% (v/v)). After filtering through the Whatman paper #3 and removing the solvents (using a rotary evaporator (BUCHI V-850)) and water (using a freeze dryer, (OPERON, FDB-5503, Korea)), the dried extracts were stored in refrigerator for further analysis.
Determination of Total Phenolic Content (TPC) Samples were measured for TPCs colorimetrically using the Folin-Ciocalteu method with modification. Absorbance was read at 725 nm against blank using UV-Visible spectrophotometer (Cecil. UK.). A calibration curve was prepared using a standard solution of Gallic acid (0.2-1mg/ml). Results were expressed as mg Gallic acid/g dry extract (mg GA/g DE).
Determination of Total Flavonoid Content (TFC). TFC was determined using the method of Huang et al. (13) with minor modifications. Absorption was measured at 430 nm using UV-VIS spectrophotometer (Cecil. UK.). TFC was determined using a standard curve with quercetin as the standard, and expressed as mg of quercetin equivalents (CE)/g dry extract (mg QE/g DE).
Determination of Total Anthocyanin Content (TAC). TAC was measured using a spectrophotometric differential pH method. Its absorbance was read at 510 and 700nm. Results were expressed as milligrams of cyanidin-3-glucoside (CY.) equivalents per g of dry extract.
Determination of Total Tannin Content. For determination of tannins in the sample extracts, vanillin–HCl method was used. The absorbance was read at 500 nm using UV–vis spectrophotometer. The content of tannins in the sample was expressed as mgcatechine equivalent (CE)/100g sample.
Determination of Antioxidant Activity. Antioxidant activity of the samples was determined using DPPH (2, 2-diphenyl-1-pic-rylhydrazyl) radical scavenging activity and ferric reducing antioxidant power (FRAP). In the presence of antioxidant, FRAP assay reduced Fe3+-TPTZ (2, 4, 6-tris (2-pyridyl)-5-triazine) complex to Fe2+ - TPTZ at low pH. The absorbance of the mixture was measured by using spectrophotometric ally at 595 nm. The effect of antioxidant on DPPH radical was thought to be due to their hydrogen donating ability or radical scavenging activity. DPPH assay expressed as IC50 and percentage inhibition. Lower IC50 value indicates higher antioxidant activity.
Results and Discussion: Efficiency of different solvent extractions depends on the matrix of plant materials as well as the type of extractable compounds. The correct selection of solvent can improve the extraction yield of antioxidants from plants matrices considerably. For this reason, in the present study, some selected types of solvent showed different results. For extraction of total phenol and flavonoid compound, acetone 50% was the best yield. In methanolic extract (50, 100%), the highest amounts of anthocyanin and total tannin were reported. In all extracts, water had the least efficiency in comparison with other solvents. High correlation was observed in total phenolic content and antioxidant activity which was determined by DPPH and FRAP assay. Acetone 50% was the most potent for scavenging free radicals and reducing a ferric-tripyridyltriazine, Fe (III)-TPTZ, complex to ferrous, Fe (II) in all extracts.
Conclusions: The results of the present study indicated that polarity, selectivity, viscosity, and vapor pressure are important physicochemical properties that should be considered when selecting a suitable solvent for the extraction of bioactive compounds from plant materials.

کلیدواژه‌ها [English]

  • Antioxidant
  • DPPH
  • Phenolic content
  • Zizyphus jujube Miller
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