ارزیابی پاسخ‌های رشدی، عملکردی و فیزیولوژیکی خیار شاخدار آفریقایی (Cucumis metuliferus L.) به تنش کم آبیاری

نوع مقاله : مقالات پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان- زنجان

2 گروه علوم باغبانی- دانشکده کشاورزی- دانشگاه زنجان

3 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

4 گروه مهندسی آب، دانشکده کشاورزی، دانشگاه زنجان، ایران

چکیده

تنش خشکی به­عنوان یک تنش غیرزیستی مهم رشد و عملکرد گیاهان را کاهش می­دهد. به منظور بررسی اثر تنش کم آبیاری بر رشد، عملکرد و شاخص­های فیزیولوژیکی خیار شاخدار آفریقایی، آزمایشی در قالب طرح بلوک­های کامل تصادفی در سه تکرار در مزرعه پژوهشی دانشگاه زنجان در سال 1398 انجام شد. سطوح آبیاری شامل سه سطح 100، 80 و 60 درصد نیاز آبی گیاه بود. نتایج نشان داد که تنش کم­آبیاری، رشد و عملکرد میوه را بطور معنی­داری کاهش داد. کمترین طول بوته، تعداد میوه (9/10) و عملکرد بوته (6/1 کیلوگرم) در تیمار کم آبیاری 60 درصد حاصل شد. وزن میوه تحت تاثیر تنش کم­آبیاری افزایش یافت و بیشترین وزن متوسط میوه (05/164 گرم) در تیمار کم آبیاری 80 درصد حاصل شد. کیفیت میوه به­طور معنی­داری تحت تاثیر تیمار آبیاری قرار گرفت. سفتی بافت میوه و مقدار ویتامین ث تحت تنش کم­آبیاری کاهش یافت و مواد جامد محلول کل، مقدار فنل کل و ظرفیت آنتی اکسیدانی میوه افزایش یافت به­طوری که بیشترین مقدار مواد جامد محلول (43/4 درصد بریکس)، فنل (6/7 میلی­گرم بر گرم وزن تر) و ظرفیت آنتی­اکسیدانی (78/48 درصد) و حداقل مقدار ویتامین ث (02/10 میلی­گرم بر 100 میلی­لیتر) و سفتی بافت میوه (73/2 کیلوگرم بر سانتی­متر مربع) در تیمار کم آبیاری 60 درصد مشاهده شد. با افزایش تنش کم­آبیاری، محتوای نسبی­آب برگ و محتوای کلروفیل، غلظت عناصر فسفر و پتاسیم برگ کاهش یافت. درصد نشت­یونی، محتوای کاروتنوئید و تجمع پرولین در پاسخ به افزایش تنش کم­آبیاری به طور معنی­داری افزایش یافت. در تیمار کم آبیاری 80 درصد، اگرچه عملکرد میوه 9/13 درصد کاهش یافت ولی در مصرف آب 20 درصد صرفه­جویی شد و با افزایش مواد جامد محلول و ظرفیت آنتی­اکسیدانی، کیفیت میوه و اندازه میوه بهبود یافت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Evaluation of Growth, Yield and Physiological Responses of African Horned Cucumber (Cucumis metuliferus L.) to Deficit Irrigation

نویسندگان [English]

  • M. Ghoreyshi 1
  • F. Nekounam 2
  • T. Barzegar 3
  • J. Nikbakht 4
1 Dept. of Horticulture Science, Faculty of Agriculture, University of Zanjan
2 Department of Horticulture, Faculty of Agriculture, University of Zanjan
3 Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
4 Water Engineering Department, Agricultural Faculty, University of Zanjan, Zanjan, Iran
چکیده [English]

Introduction
Water stress is the most prominent abiotic stress limiting agricultural crop growth and productivity. Deficit irrigation stress as a consequence of the progressive decrease in water availability has been a hot topic regarding food security during the last two decades. Growth and development of plants is influenced by reduction in turgor that results in decreased nutrient acquisition from dry soil. When water supply is limited, plant growth and yield is reduced and plant structure is modified by decreasing in leaf size. The effect of deficit irrigation on fruit yield and quality has been reported by numerous researchers with different results. In melon, deficit irrigation reduced marketable fruit number and yield, average fruit weight, fruit diameter and did not affect rind thickness and seed cavity, but increased total soluble solids content. Although the effects of water stress have been studied on growth and yield of different crops during the last years, recent information on the response of African horned cucumber yield and quality to deficit irrigation remains limited, particularly about the results of restricted water distributions in arid and sub-arid environments. The main goal of this study was to evaluate the effect of controlled deficit irrigation on growth, physiological parameters and yield and fruit quality of African horned cucumber.
 
Material and Methods
Field experiment was performed based on a completely randomized block design with three irrigation regimes (60, 80 and 100 %ETc), whit three replications at Research Farm of University of Zanjan during the 2019. The African horned cucumber seeds were sown on 1th July 2020 at recommended spacing of 50 cm in row with 120 cm between rows. The irrigation system consisted of one drip line every crop row. The three irrigation levels were calculated based on actual evapotranspiration (ETc): (1) control, irrigated 100% crop water requirement, (2) deficit irrigation 80% ETc and (3) deficit irrigation 60% ETc. The Water requirement of the plant for control treatment was estimated using long-term average daily data of meteorological parameters recorded at Zanjan Meteorological Station and following relation. Before starting the differential irrigation at five-leaf stage, all treatments were supplied with similar amount of water to maximize stands and uniform crop establishment. During plant growth, the relative water content, proline content, electrolyte leakage, chlorophyll and carotenoids, P and K contents were measured. After fruit harvest, vine length of each plant, leaf dry weight and stem diameter were measured. The fruits were harvested when color changed from green to yellow. Fruit weight, fruit number per plant and fruit yield per plant was measured. Immediately after harvest, fruit firmness, total soluble solid, total phenols content, antioxidant capacity and vitamin C were determined.
 
 
Results and Discussion
As the results showed water deficit stress significantly reduced plant length, chlorophyll content, and increased carotenoids content. Based on the findings, deficit irrigation caused a significant reduction in leaf relative water content. According to the results, phosphorus and potassium contents in African horned cucumber leaves decreased with deficit irrigation treatments. The highest P and K contents were found under irrigation 100 %ETc treatment. Drought stress and associated reduction in soil moisture can decrease plant nutrient uptake by reducing nutrient supply through mineralization. The proline content increased with the deficit irrigation treatments; in particular with sever deficit irrigation (60 %ETc). Mean comparisons of data showed that deficit irrigation led to a significant increase in electrolyte leakage compared to control.
Water deficit stress caused significant reductions in yield. The highest fruit number per plant and yield were obtained under irrigation 100% ETc. The average fruit weigh significantly increased in response to increase water deficit stress. Deficit irrigation treatments significantly decreased vitamin C and fruit firmness. Significant differences among irrigation treatments were observed for total phenols and total soluble solid contents. The phenols and total soluble solid contents increased with the decrease of irrigation water applied. Antioxidant capacity was affected significantly by the irrigation treatments, and water deficit stress increased antioxidant capacity, which no significant difference was observed between irrigation 100 and 80 %ETc.
 
Conclusion
Water deficit has been shown to adversely affect plant growth, fruit yield, and leaf water status of African horned cucumber, but led to increase the TSS and antioxidant capacity. According to the results, fruit yield reduced 13.9 % under irrigation 80% ETc compared to irrigation 100% ETc, However, water consumption was saved by 20% and improved fruit weight and fruit quality with increasing soluble solids and antioxidant capacity.

کلیدواژه‌ها [English]

  • Antioxidant capacity
  • Electrolyte leakage
  • Fruit weight
  • Proline
  • Yield
  1. Abdalla, M.M., & El-Khoshiban, N.H. (2007). The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticum aestivum Journal of Applied Science Research, 3, 2062-2074.
  2. Al-Ghobari, H.M., Mohammad, F.S., & El-Marazky, M.S.A. (2013). Effect of intelligent irrigation on water use efficiency of wheat crop in arid region. Journal of Animal and Plant Sciences, 23(6), 1691-1699.
  3. Anjali, S., & Kale, P.B. (2007). Response and recovery of Coriandrum sativum variety indoor exposed to soil moisture stress. Indian Journal of Plant Physiology, 12, 266-270.
  4. (2000). Official method of analysis of the association of official analytical chemists. Washington D.C. 12: 377-378.
  5. Apel, K., & Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Plant Biology, 55, 373-399. http://dx.doi.org/10.1146/annurev.arplant.55.031903.141701
  6. Arnon, A.N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.
  7. Ashraf, M., & Foolad, M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206-216. http://dx.doi.org/10.1016/j.envexpbot.2005.12.006
  8. Babalar, M., Dolati Baneh, A., & Shraftyan, D. (1998). Effect of calcium chloride on the quality of post-harvest storage of two varieties of grape and currant Shahroudi. Iranian Journal of Horticultural Science 15(1), 31-40. (In Persian with English abstract)
  9. Barzegar, T., Fateh, M., & Razavi, F. (2018a). Enhancement of postharvest sensory quality and antioxidant capacity of sweet pepper fruits by foliar applying calcium lactate and ascorbic acid. Scientia Horticulturae, 241, 293–303. https://doi.org/10.1016/j.scienta.2018.07.011
  10. Barzegar, T., Heidaryan, N., Lotfi, H., & Ghahremani, Z. (2018b). Yield, fruit quality and physiological responses of melon cv. Khatooni under deficit irrigation. Advances in Horticultural Science, 32(4), 451-458. https://doi.org/10.13128/ahs-22456
  11. Barzegar, T., Moradi, P., Nikbakht, J., & Ghahremani, Z. (2016). Physiological response of okra cv. Kano to foliar application of putrescine and humic acid under water deficit stress. International Journal of Horticultural Science and Technology, 3(2), 187-197. https://doi.org/10.22059/ijhst.2017.213448.147
  12. Bates, L.W., Aldren, R.P., & Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207.
  13. Ben Hamed, K., Castagna, A., Salem, E., Ranieri, A., & Abdelly, C. (2007). Sea fennel (Crithmum maritimum) under salinity conditions: a comparison of leaf and root antioxidant responses. Plant Growth Regulators, 53, 185-194.
  14. Bista, D.R., Heckathorn, S.A., Jayawardena, D.M., Mishra, S., & Boldt, J.K. (2018). Effects of drought on nutrient uptake and the levels of nutrient-uptake proteins in roots of drought-sensitive and -tolerant grasses. Plants, 7(2), 1-16. https://doi.org/10.3390/plants7020028
  15. Dehghan, G., & Khoshkam, Z. (2012). Tin (II)–quercetin complex: Synthesis, spectral characterization and antioxidant activity. Food Chemistry, 131(2), 422-426. https://doi.org/1016/j.foodchem.2011.08.074
  16. Demiray, E., Tulek, Y., & Yilmaz, Y. 2013. Degradation kinetics of lycopene, β-carotene and ascorbic acid in tomatoes during hot air drying. LWT-Food Science and Technology, 50(1), 172-176. https://doi.org/10.1016/j.lwt.2012.06.001
  17. Dong, C.X., Zhou, J.M., Fan, X.H., Wang, H.Y., Duan, Z.Q., & Tang, C. (2005). Application methods of calcium supplements affect nutrient levels and calcium forms in mature tomato fruits. Journal of Plant Nutrition, 27, 1443-1455. https://doi.org/10.1081/PLN-200025861
  18. Edreva, A. (2005). The importance of non-photosynthetic pigments and cinnamic acid derivatives in photoprotection. Agriculture Ecosystems and Environment, 106(2), 135-146. https://doi.org/10.1016/j.agee.2004.10.002
  19. Erdem, Y., Shirali, S. Erdem, T., & Kenar, D. (2006). Determination or crop water stress index for irrigation scheduling of Bean (Phaseolus vulgaris). Journal Agriculture and Forest, 30, 195-202.
  20. Fabeiro, C., Martin de Santa Olalla, F., & De Juan, J.A. (2002). Production of muskmelon (Cucumis melo) under controlled deficit irrigation in a semi-arid climate. Agricultural Water Management, 54, 93-105. https://doi.org/10.1016/S0378-3774(01)00151-2
  21. Farooq, M., Somasundaram, R., & Panneerselvam, R. (2012). Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agricultural and Biological Engineering, 11, 100-105.
  22. Ferrara, L. (2018). A fruit to discover: Cucumis metuliferusMey Ex Naudin (Kiwano). Journal of Clinical Nutrition and Metabolism, 5, 1–2. https://doi.org/10.15761/CNM.1000109
  23. Ge, T.D., Sun, N.B., Bai, L.P., Tong, C.L., & Sui, F.G. (2012). Effects of drought stress on phosphorus and potassium uptake dynamics in summer maize (Zea mays) throughout the growth cycle. Acta Physiology of Plantarum, 34, 2179–2186. https://doi.org/10.1007/s11738-012-1018-7
  24. Ghahremani, Z., Mikaealzadeh, M., Barzegar, T., & Ranjbar, M.E. (2021). Foliar application of ascorbic acid and gamma aminobutyric acid can improve important properties of deficit irrigated cucumber plants (Cucumis sativus Us). Gesunde Pflanzen, 73, 77–84. https://doi.org/10.1007/s10343-020-00530-6
  25. Ghorbanli, M., Gafarabad, M., Amirkian, T., & Allahverdi Mamaghani, B. (2013). Investigation of proline, total protein, chlorophyll, ascorbate and dehydroascorbate changes under drought stress in Akira and Mobil tomato cultivars. Iranian Journal of Plant Physiology, 3, 651-658.
  26. Jackson, M.L. (1958). Soil chemical analysis, Prentice Hall Inc, Englewood Cliffs, New Jersey. United States of America, 498.
  27. Harb, A., Krishnan, A., Ambavaram, M.M., & Pereira, A. (2010). Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiology, 154(3), 1254-1271. https://doi.org/10.1104/pp.110.161752
  28. Hong, W., & Jj-yun, J. (2007). Effects of zinc deficiency and drought on plant growth and metabolism of reactive oxygen species in maize (Zea mays). Agricultural Sciences in China, 6, 988-995. https://doi.org/10.1016/S1671-2927(07)60138-2
  29. Hoseini, S.Z., Barzegar, T., Nikbakht, J., & Ghahremani, Z. (2018). Growth and physiological reactions of common bean cv. Sanry in response to salicylic acid and biostimulants under different irrigation regimes. Journal of Plant Ecophysiology, 10(35), 73-87.
  30. Ivan Garcia, T., Victor Hugo, D.Z., & Jose Luis, M.F. (2011). Long-term impact of sustained deficit irrigation on yield and fruit quality in sweet orange cv. Salustiana (SW Spain). Comunicata Scientiae, 2(2), 76-84. https://doi.org/10.14295/cs.v2i2.41
  31. Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A., & Nabati, J. (2009). Physiology of environmental stresses in plants. Publication of Ferdowsi University. 502 pp. (in Persian).
  32. Kavas, M., Cengiz, M., & Akca, O. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings'. Turkish Journal of Biology, 37, 491-498. https://doi.org/10.3906/biy-1210-55
  33. Khani, A., Barzegar, T., Nikbakht, J., & Ghahremani, Z. (2020) Effect of foliar spray of calcium lactate on the growth, yield and biochemical attribute of lettuce (Lactuca sativa) under water deficit stress. Advances in Horticultural Science, 34(1), 11-24. https://doi.org/10.13128/ahsc-8252
  34. Kusvuran, S., Dasgan, H.Y., & Abak, K. (2011). Responses of different melon genotypes to drought stress. Journal of Agriculture Science, 21, 209-219.
  35. Liu, J.J., Lin, S.H., Xu, P.L., Wang, X.J., & Bai, J.G. (2009). Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. Agricultural Sciences in China, 8(9), 1075-1086. https://doi.org/10.1016/S1671-2927(08)60315-6
  36. Lum, M., Hanafi, M., Rafii, Y., & Akmar, A. (2014). Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice. Journal of Animal and Plant Sciences, 24, 1487–1493.
  37. Mahmoudnia, M.M., Farsi, M., Marashi S., & Ebadi, P. (2013). Physiological response to drought stress in four species of tomato. Journal of Horticultural Science, 26(4), 409-416. https://doi.org/10.22067/jhorts4.v0i0.18252
  38. Maluleke, M.K., Moja, S.J., Nyathi, M., & Modise, D.M. (2021). Nutrient concentration of african horned cucumber (Cucumis metuliferus L) fruit under different soil types, environments, and varying irrigation water levels. Horticulturae, 7(4), 76. https://doi.org/10.3390/horticulturae7040076
  39. Najarian, M., Mohammadi-Ghehsareh, A., Fallahzade, J., & Peykanpour, E. (2018). Responses of cucumber (Cucumis sativus) to ozonated water under varying drought stress intensities. Journal of Plant Nutrition, 41(1), 1-9. https://doi.org/10.1080/01904167.2017.1346665
  40. Naz, H., Akram, N.A., & Ashraf, M. (2016). Impact of ascorbic acid on growth and physiological attributes of cucumber (Cucumis sativus) plants under water-deficit condition. Pakistan Journal of Botany, 48(3): 877-883.
  41. Orabi, S.A., Salman, S.R., & Shalaby, M.A. (2010). Increasing resistance to oxidative damage in cucumber (Cucumis sativus) plants by exogenous application of salicylic acid and paclobutrazol. World Journal of Agricultural Sciences, 6, 252-259.
  42. Parkhideh, P., Barzegar, T., Nikbakht, J., & Nekonam, F. (2018a). The evaluate of growth, yield and physiological responses of bitter apple (Citrullus colocynthis) under deficit irrigation stress condition. Crops Improvement (Journal of Agricultural Crops Production), 20(2), 357-369. (In Persian with English abstract). https://doi.org/10.22059/jci.2018.225366.1641
  43. Parkhideh, P., Barzegar, T., & Nekonam, F. (2018b). Growth, yield and physiological responses of watermelon cv. Charleston Gray grafted on bitter apple (Citrullus colocynthis ) rootstock under deficit irrigation stress. Iranian Journal of Horticultural Science, 49(2), 539-550. (In Persian with English abstract). https://doi.org/10.22059/ijhs.2017.233823.1258
  44. Ramroudi, M., & Khomr, A.R. (2013). Interaction effects of salicylic acid spraying and different irrigation levels on some quantity and quality traits, and osmoregulators in basil (Ocimum basilicum). Applied Research of Plant Ecophysiology, 1(1), 19-31.
  45. Ritchie, S.W., Nguyen, H.T., & Holaday, A.S. (1990). Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30(1), 105-111. https://doi.org/10.2135/cropsci1990.0011183X003000010025x
  46. Shao, H.B., Chu, L.Y., Jaleel, C.A., & Zhao, C.X. (2008). Water-deficit stress induced anatomical changes in higher plants. Comptes Rendus Biologies, 331, 215-225. https://doi.org/10.1016/j.crvi.2008.01.002
  47. Singleton, V., & Rossi, J. (1965). Colorimetry of total phenolic compounds with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
  48. Sharma, S.P., Leskovar, D.I., Crosby, K.M., Volder, A., & Ibrahim, A.M.H. (2014). Root growth, yield, and fruit quality responses of reticulatus and inodorus melons (Cucumis melo) to deficit subsurface drip irrigation. Agricultural Water Management, 136, 75-85. https://doi.org/10.1016/j.agwat.2014.01.008
  49. Shaw, B., Thomas, T.H., & Cooke, D.T. (2002). Responses of sugar beet (Beta vulgaris) to drought and nutrient deficiency stress. Plant Growth Regulation, 37, 77-83. https://doi.org/10.1023/A:1020381513976
  50. Shi, J., Zuo, J., Zhou, F., Gao, L., Wang Q., & Aili Jiang, A. (2018). Low-temperature conditioning enhances chilling tolerance and reduces damage in cold-stored eggplant (Solanum melongena) fruit. Postharvest Biology and Technology, 141, 33–38. https://doi.org/10.1016/j.postharvbio.2018.03.007
  51. Suyum, K., Dasgan, H.Y., Sari, N., & Kusvuran, S. (2012). Genotypic variation in the response of watermelon genotypes to salinity and drought stresses. In: Proceedings of the 15th National Vegetable Symposium, Konya-Turkey. pp.225-230.
  52. Vaziri, Z.H., Salamat, A., Ansari, M., Meschi, M., Heidari, N., & Dehqany Sanych, H. (2009). Evapotranspiration plant (water consumption guidelines for plants) (Translation). Publications of the National Committee of Irrigation and Drainage, printing, Tehran. (In Persian)
  53. Wang, J., Huang, G., Li, J., Zheng, J., Huang, Q., & Liu, H. (2017). Effect of soil moisture-based furrow irrigation scheduling on melon (Cucumis melo) yield and quality in an arid region of Northwest China. Agricultural Water Management, 179, 167-176. https://doi.org/10.1016/j.agwat.2016.04.023
  54. Zulu, N.S. (2009). Wild watermelon (Citrullus lanatus L.) landrace production in response to three seedling growth media and field planting dates. M.Sc. thesis. Faculty of Agriculture KwaZulu-Nata University, Pietermaritzburg.
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