قابلیت تلاقی سه گونه قارچ صدفی (Pleurotus spp.) و ارزیابی خصوصیات هیبریدهای حاصل

مقالات آماده انتشار

نوع مقاله : مقالات پژوهشی

نویسندگان

• مرتضی تقوی شربیانی ¹
• علیرضا مطلبی آذر ¹
• جابر پناهنده ²
• امین جهانیان ³

¹ 1- گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تبریز، ایران

² گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تبریز، ایران

³ دانشگاه تبریز

10.22067/jhs.2025.91744.1406

چکیده

چکیده

مهم‌ترین برنامه توسعه هر کشوری تأمین منابع غذایی کافی است و با توجه به افزایش بی رویه جمعیت، تنها با استفاده از روش‌های کشاورزی سنتی نمی‌توان بر مشکل کمبود مواد غذایی غلبه کرد. امروزه قارچ‌های خوراکی یکی از منابع مهم تأمین مواد غذایی در دنیا محسوب می شوند و کشت آنها با افزایش جمعیت به طور پیوسته در حال افزایش است. از این رو اهمیت کارهای اصلاحی بر روی قارچ های خوراکی روز به روز بیشتر احساس می‌شود در این تحقیق سه گونه قارچ صدفی (Pleurotus osturatus var florida, P. eryngii, P. corncopia) به منظور بهره‌مندی از صفات ویژه هر گونه و تجمع آنها در یک گونه، با همدیگر تلاقی داده شدند. منوکاریون هر یک از گونه‌ها تهیه شد. سپس منوکاریون دو گونه به فاصله 5 میلی‌متر از هم قرار داده شدند. برای کشت اسپان هیبریدها و والدین برای هر کدام سه بستر حاوی کلش گندم (به وزن 8/6 کیلوگرم) تهیه گردیده و استریل شدند. نتایج نشان داد از بین 300 تلاقی، فقط در 4 تلاقی، میسیلیوم منوکاریون‌های فلوریدا و ارنجی به‌طرف هم رشد کرده و با تشکیل قوص اتصال، دی کاریون تولید کردند و چهار هیبرید به‌نام‌های H1 ,وH40 ,H11 ,H32 به‌دست آمد. در حالی که از بین 300 تلاقی بین منوکاریون‌های فلوریدا با قارچ زرد و نیز 100 تلاقی بین منوکاریون‌های ارنجی با قارچ زرد به علت ناسازگاری بین گونه‌ای، هیچ دی کاریونی تولید نشد. اسپان هیبرید‌های تولیدی به همراه فلوریدا و ارنجی، تهیه شد و سپس در بسترهای استریل به‌صورت طرح کاملاً تصادفی در سه تکرار کشت شدند. از لحاظ پنجه دوانی بین ژنوتیپ‌ها اختلاف قابل ملاحظه‌ای وجود داشت و هیبریدها از این لحاظ به ارنجی شبیه بوده و در مدت زمان طولانی‌تری نسبت به فلوریدا پنجه دوانی کردند. هیبرید H40 و H32 به‌خاطر اینکه از تعداد روز تا ظهور اولین پین و نهایتاً از طول دوره کاشت کمتری برخوردار بودند، زودرس‌تر از والدین و سایر هیبریدها بودند. هیبرید H40 با دارا بودن وزن تر اندام خوراکی مشابه با ارنجی یک هیبرید مناسب محسوب می‌گردد. هیبرید H40 از عملکرد (وزن تر) و بیوماس بیشتری نسبت به هیبریدهای دیگر برخوردار بوده و مشابه ارنجی بود. حداقل اسپور زایی در هیبریدهای H1 وH11 مشاهده گردید که این مقدار اسپور زایی حتی کمتر از ارنجی بود. هیبرید H40 هر چند از برخی صفات هیبرید مطلوبی به نظر می‌رسد اما از اسپور زایی زیاد و مشابه فلوریدا بخوردار بود.

کلیدواژه‌ها

• عملکرد
• ارنجی
• فلوریدا
• قارچ زرد
• هیبرید بین گونه‌ای

موضوعات

• سبزیکاری

عنوان مقاله [English]

Cross compatibility of three oyster mushroom species (Pleurotus sp.) and evaluation of the characteristics of the resulting hybrids

نویسندگان [English]

• morteza taghavi sharabiani ¹
• alireza motallebiazar ¹
• jaber panahandeh ²
• amin jahanian ³

¹ Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

² Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

³ university of Tabriz

چکیده [English]

Abstract

Introduction: The most important development plan of any country is to provide sufficient food resources, and with uncontrolled population growth. Also, the problem of food shortage cannot be solved by traditional agricultural methods alone. Mushrooms are a very large group of organisms. The number of species of mushrooms is estimated at about 1.5 million, of which about 300 are edible, only 30 have been domesticated, and finally 10 species are cultivated commercially. Oyster mushrooms of the genus Pleurotus are the most important cultivated edible mushrooms in the world after button mushrooms. Edible mushrooms are now considered one of the most important food sources in the world, and their cultivation is constantly increasing with population growth. For this reason, the importance of cultivating edible mushrooms is increasing day by day. In edible mushrooms, the mycelium that emerges from a single spore is sterile and requires hybridization between compatible monokaryonic mycelia to complete the sexual cycle. The oyster mushroom P. eryngii has also been given the name of king oyster mushroom due to its large edible organ. The mushrooms grow in small groups. The spores of this mushroom are white, and its mycelium has a connecting arch. The king oyster mushroom has a firm texture and short radial blades.

Materials and methods: In this study, three species of oyster mushrooms (Pleurotus ostratus var florida, P. eryngii, P. corncopia) were crossed with each other in order to benefit from the special characteristics of each species and their aggregation into one species. Monokaryons of each species were prepared. Then, monokaryons of the two species were placed 5 mm from each other. For the cultivation of hybrid spawn and parents, three beds containing wheat straw (weighing 6.8 kg) were prepared for each and sterilized. After spawning, the beds were placed in a suitable environment and then the humidity of the environment was increased to 85-90% until the hybrids started to produce primary nodes and then pin and turn into mature heads. The heads at this stage were tested for spore production.

Results and discussion: The results showed that from 300 crosses, only in 4 crosses did the Florida and eryngii monokaryons grow together and form a clamp connection, producing dikaryons, and four hybrids named H1, H40, H11, and H32 were obtained. While from amoung 300 crosses between Florida monokaryons and yellow fungus and 100 crosses between eryngii monokaryons and yellow fungus, no dikaryons were produced due to interspecific incompatibility. Spawns of the produced hybrids were prepared with Florida and eryngii and then cultivated in sterile media in a completely randomized design with three replications. The average base diameter of eryngii was significantly greater than that of Florida. The average base diameter of eryngii was not significantly different from that of the H11 hybrid, while the average base diameter of eryngii was greater than that of the H32 and H11 hybrids and less than that of the H1 hybrid. There was also a significant difference in the average base diameter among the hybrids. Among the hybrids, H1 had a larger average base diameter than that of the H40, H32, and H11 hybrids. eryngii had the highest fresh weight of edible organs with an average fresh weight of 120.5 grams. The average fresh weight of edible organs of eryngii was not significantly different from the hybrid H40. In terms of yield (fresh and dry weight), the hybrids H11, H1 and H32 were very similar and were placed in a single cluster. On the other hand, the hybrid H40 was similar to eryngii. In general, the hybrids and eryngii were placed in the same group in terms of yield. This is while Florida occupied a separate group. The result is that in terms of yield, the hybrids were similar to eryngii and the high yield traits from Florida were not transferred to the hybrids.

Conclusion: There was a significant difference between genotypes in terms of spawn running, and the hybrids were similar to eryngii and spawn running for a longer period than Florida. Hybrids H40 and H32 were earlier than their parents and other hybrids because they had shorter days to first pin emergence and ultimately shorter planting period. Hybrid H40 is considered a suitable hybrid with similar edible organ fresh weight to eryngii. Hybrid H40 had higher yield (fresh weight) and biomass than other hybrids and was similar to eryngii. The least sporulation (spore germination) was observed in hybrids H1 and H11, which was even lower than eryngii. Hybrid H40, although it seems to be a desirable hybrid in some traits, had high spore germination and was similar to Florida.

کلیدواژه‌ها [English]

• Yield
• eryngii
• Florida
• Yellow Mushroom
• Interspecific Hybrid