تأثیر اسید‌‌ سالیسیلیک و تنش شوری بر جوانه‌زنی بذر و آنتی‌اکسیدان‌های فراسودمند در جوانه‌های کلم براکلی

نوع مقاله : مقالات پژوهشی

نویسندگان

1 دانشگاه تهران

2 پژوهشکده بیوتکنولوژی منطقه مرکزی کشور

چکیده

سولفورافان ماده‌ای بسیار قوی در کنترل سرطان می‌باشد. این ماده در کلم براکلی تولید می‌شود و به نظر می‌رسد تنش شوری از طریق فعال کردن مقاومت اکتسابی سیستمیک باعث بیش‌تر شدن تولید سولفورافان گردد. با هدف به‌دست آوردن غلظت مناسب شوری برای تولید حداکثر سولفورافان، به‌همراه کنترل درصد جوانه‌زنی، تیمار با NaCl در 5 سطح0، 50، 100، 150، 200 میلی‌مولار به‌همراه اسیدسالیسیلیک (0 ، 100،200 میکرومولار) مورد آزمایش قرار گرفت. برای بررسی تأثیر تیمارها بر حداکثر درصد جوانه‌زنی، سرعت افزایش و زمان لگ، مدل لجستیک استفاده شد و سولفورافان و ویتامین ث با استفاده از HPLC اندازه‌گیری شدند. اسید سالیسیلیک مقاومت به شوری را افزایش داد. ترکیب اسید سالیسیلیک و کلرید سدیم غلظت سولفورافان و ویتامین‌ث را افزایش داد. تیمار100 میکرومولار اسید سالیسیلیک به‌همراه 100 میلی‌مولار کلرید سدیم بالاترین غلظت سولفورافان و ویتامین ث، به‌همراه بهترین فاکتورهای رشدی را ایجاد نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Salicylic Acid and Salt Stress on Seed Germination and Functional Antioxidant in Broccoli Sprout

نویسندگان [English]

  • M. Babalar 1
  • J. Hashemi 2
  • Y. Mostufi 1
1 University of Tehran
2 Agriculture Biotechnology research institute of Iran
چکیده [English]

Sulforaphane is a strong anticarsenogen compound which is produced in broccoli. It seems salt stress improves sulforaphane production via systemic acquired resistance. To obtain suitable salt concentration and for having more content of sulforaphane with controlling germination percentage, NaCl in 5 levels 0, 50, 100, 150, 200 mM (0, 4.53, 9.125, 13.68, 18.25 dS/m ) and salicylic acid in 3 levels (0,100, 200 µM) were examined. A logistic regression model was used to assess the effects of treatments on the maximum cumulative germination percentages, rate of increase, and germination lag times. Sulphoraphan and vitamin C were determind by HPLC method. Combination between salicylic acid and chloride sodium improved sulforaphane concentration. The 100 µM salicylic acid with 100 mM chloride sodium was the best treatment as the highest concentration of sulforaphane and vitamin C with perfect growth factors achieved in the treatment.

کلیدواژه‌ها [English]

  • Hydroxy benzoic acid
  • Sodium Chloride
  • Germination factors
  • Sulforaphane
  • vitamin C
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